ATE365203T1 - Hefe-extraktlösung, ihre herstellung und verwendung zur zellfreien proteinsynthese - Google Patents

Hefe-extraktlösung, ihre herstellung und verwendung zur zellfreien proteinsynthese

Info

Publication number
ATE365203T1
ATE365203T1 AT03029972T AT03029972T ATE365203T1 AT E365203 T1 ATE365203 T1 AT E365203T1 AT 03029972 T AT03029972 T AT 03029972T AT 03029972 T AT03029972 T AT 03029972T AT E365203 T1 ATE365203 T1 AT E365203T1
Authority
AT
Austria
Prior art keywords
cell
protein synthesis
free protein
extract solution
yeast extract
Prior art date
Application number
AT03029972T
Other languages
English (en)
Inventor
Takashi Suzuki
Toru Ezure
Masaaki Ito
Original Assignee
Shimadzu Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shimadzu Corp filed Critical Shimadzu Corp
Application granted granted Critical
Publication of ATE365203T1 publication Critical patent/ATE365203T1/de

Links

Classifications

    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04M—TELEPHONIC COMMUNICATION
    • H04M1/00—Substation equipment, e.g. for use by subscribers
    • H04M1/60—Substation equipment, e.g. for use by subscribers including speech amplifiers
    • H04M1/6033—Substation equipment, e.g. for use by subscribers including speech amplifiers for providing handsfree use or a loudspeaker mode in telephone sets
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C12N1/063—Lysis of microorganisms of yeast
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04R—LOUDSPEAKERS, MICROPHONES, GRAMOPHONE PICK-UPS OR LIKE ACOUSTIC ELECTROMECHANICAL TRANSDUCERS; ELECTRIC HEARING AIDS; PUBLIC ADDRESS SYSTEMS
    • H04R1/00—Details of transducers, loudspeakers or microphones
    • H04R1/10—Earpieces; Attachments therefor ; Earphones; Monophonic headphones
    • H04R1/1041—Mechanical or electronic switches, or control elements
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04R—LOUDSPEAKERS, MICROPHONES, GRAMOPHONE PICK-UPS OR LIKE ACOUSTIC ELECTROMECHANICAL TRANSDUCERS; ELECTRIC HEARING AIDS; PUBLIC ADDRESS SYSTEMS
    • H04R2499/00—Aspects covered by H04R or H04S not otherwise provided for in their subgroups
    • H04R2499/10—General applications
    • H04R2499/11—Transducers incorporated or for use in hand-held devices, e.g. mobile phones, PDA's, camera's
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04R—LOUDSPEAKERS, MICROPHONES, GRAMOPHONE PICK-UPS OR LIKE ACOUSTIC ELECTROMECHANICAL TRANSDUCERS; ELECTRIC HEARING AIDS; PUBLIC ADDRESS SYSTEMS
    • H04R3/00—Circuits for transducers

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Signal Processing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT03029972T 2003-01-07 2003-12-30 Hefe-extraktlösung, ihre herstellung und verwendung zur zellfreien proteinsynthese ATE365203T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2003001317A JP2004208640A (ja) 2003-01-07 2003-01-07 無細胞系タンパク質合成用酵母抽出液およびその調製方法、ならびにそれを用いた無細胞系タンパク質合成方法

Publications (1)

Publication Number Publication Date
ATE365203T1 true ATE365203T1 (de) 2007-07-15

Family

ID=32501191

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03029972T ATE365203T1 (de) 2003-01-07 2003-12-30 Hefe-extraktlösung, ihre herstellung und verwendung zur zellfreien proteinsynthese

Country Status (7)

Country Link
US (1) US7387884B2 (de)
EP (1) EP1437399B1 (de)
JP (1) JP2004208640A (de)
KR (1) KR20040063809A (de)
CN (1) CN1530373A (de)
AT (1) ATE365203T1 (de)
DE (1) DE60314491T2 (de)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4091901B2 (ja) * 2003-11-13 2008-05-28 株式会社島津製作所 無細胞系タンパク質合成におけるミクロソーム膜添加による翻訳後修飾方法
WO2014144583A2 (en) 2013-03-15 2014-09-18 Northwestern University Methods for cell- free protein synthesis
BR112016024635A2 (pt) * 2014-04-24 2017-08-15 Rapid Micro Biosystems Inc meios de crescimento microbiológico e métodos de uso do mesmo
WO2017117539A1 (en) 2015-12-30 2017-07-06 Northwestern University Cell-free glycoprotein synthesis (cfgps) in prokaryotic cell lysates enriched with components for glycosylation
US10829795B2 (en) 2016-07-14 2020-11-10 Northwestern University Method for rapid in vitro synthesis of glycoproteins via recombinant production of N-glycosylated proteins in prokaryotic cell lysates
CN106978349B (zh) * 2016-09-30 2018-06-22 康码（上海）生物科技有限公司 一种体外蛋白质合成的试剂盒及其制备方法
CN108535489B (zh) * 2017-03-04 2019-04-19 康码（上海）生物科技有限公司 一种用于体外蛋白质合成的蛋白合成体系、试剂盒及其制备方法
JP2020127364A (ja) * 2017-04-28 2020-08-27 Ｓｐｉｂｅｒ株式会社 無細胞タンパク質合成用反応混合物、これを用いた無細胞タンパク質合成方法、及び無細胞タンパク質合成用キット
CN107299058A (zh) * 2017-07-21 2017-10-27 天津大学 一种用于无细胞蛋白质合成的细胞提取物及其制备方法
CN109321620A (zh) * 2017-07-31 2019-02-12 康码（上海）生物科技有限公司 一种蛋白合成冻干制剂及其制备方法和应用
WO2019035916A1 (en) 2017-08-15 2019-02-21 Northwestern University DESIGN OF PROTEIN GLYCOSYLATION SITES BY RAPID EXPRESSION AND CHARACTERIZATION OF N-GLYCOSYLTRANSFERASES
US11051532B2 (en) 2017-09-22 2021-07-06 Impossible Foods Inc. Methods for purifying protein
US11530432B2 (en) 2018-03-19 2022-12-20 Northwestern University Compositions and methods for rapid in vitro synthesis of bioconjugate vaccines in vitro via production and N-glycosylation of protein carriers in detoxified prokaryotic cell lysates
WO2019204346A1 (en) 2018-04-16 2019-10-24 Northwestern University METHODS FOR CO-ACTIVATING IN VITRO NON-STANDARD AMINO ACID (nsAA) INCORPORATION AND GLYCOSYLATION IN CRUDE CELLLYSATES
WO2020040840A2 (en) 2018-06-01 2020-02-27 Northwestern University Expanding the chemical substrates for genetic code reprogramming
US12421537B2 (en) 2018-07-06 2025-09-23 Northwestern University Ribosome variants for sequence defined polymer synthesis
US12098433B2 (en) 2018-08-03 2024-09-24 Northwestern University On demand, portable, cell-free molecular sensing platform
CN109321547B (zh) * 2018-10-08 2021-01-15 广东菲鹏生物有限公司 一种RNasin的制备方法
EP3670646A1 (de) * 2018-12-21 2020-06-24 Ohly GmbH Funktionelles hefeproteinkonzentrat
CN113646438A (zh) 2019-01-11 2021-11-12 西北大学 在原核生物细胞裂解物中合成生物缀合物疫苗
US12325884B2 (en) 2019-03-04 2025-06-10 Northwestern University Riboswitch-based fluoride sensing in cell-free extract
US12226410B2 (en) 2019-10-18 2025-02-18 Northwestern University Methods for enhancing cellular clearance of pathological molecules via activation of the cellular protein ykt6
CN115135771A (zh) 2019-10-25 2022-09-30 西北大学 用于富集膜泡和增加糖蛋白产量的无细胞提取物制备方案
US12011016B2 (en) 2020-09-14 2024-06-18 Impossible Foods Inc. Protein methods and compositions

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE424467T1 (de) * 1999-05-11 2009-03-15 Cellfree Sciences Co Ltd Zubereitung, die einen zellextrakt zur herstellung eines zellfreien proteins enthalten und vorrichtung zur herstellung eines zellfreien proteins
JP2002125693A (ja) * 2000-10-19 2002-05-08 Toyobo Co Ltd 無細胞タンパク質合成用細胞抽出液組成物
JP4750299B2 (ja) * 2001-03-08 2011-08-17 独立行政法人理化学研究所 無細胞タンパク質合成系によるタンパク質の製造方法

Also Published As

Publication number Publication date
KR20040063809A (ko) 2004-07-14
JP2004208640A (ja) 2004-07-29
US20040137560A1 (en) 2004-07-15
DE60314491D1 (de) 2007-08-02
US7387884B2 (en) 2008-06-17
EP1437399A1 (de) 2004-07-14
EP1437399B1 (de) 2007-06-20
DE60314491T2 (de) 2008-02-21
CN1530373A (zh) 2004-09-22

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