ATE369439T1 - Markierter primer, geeignet für die detektion von nukleinsäuren - Google Patents
Markierter primer, geeignet für die detektion von nukleinsäurenInfo
- Publication number
- ATE369439T1 ATE369439T1 AT98123569T AT98123569T ATE369439T1 AT E369439 T1 ATE369439 T1 AT E369439T1 AT 98123569 T AT98123569 T AT 98123569T AT 98123569 T AT98123569 T AT 98123569T AT E369439 T1 ATE369439 T1 AT E369439T1
- Authority
- AT
- Austria
- Prior art keywords
- labeled
- primer
- dna sequence
- detection
- nucleic acids
- Prior art date
Links
- 238000001514 detection method Methods 0.000 title 1
- 150000007523 nucleic acids Chemical class 0.000 title 1
- 102000039446 nucleic acids Human genes 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 title 1
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 3
- 239000000975 dye Substances 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 238000003752 polymerase chain reaction Methods 0.000 abstract 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 abstract 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 abstract 1
- 108091034117 Oligonucleotide Proteins 0.000 abstract 1
- 230000003321 amplification Effects 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
- 239000007850 fluorescent dye Substances 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 230000001915 proofreading effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6846—Common amplification features
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6823—Release of bound markers
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19755642A DE19755642B4 (de) | 1997-12-15 | 1997-12-15 | Markierter Primer für die Polymerasekettenreaktion |
| DE19806850A DE19806850A1 (de) | 1998-02-19 | 1998-02-19 | Markierter Primer für die Polymerasekettenreaktion |
| DE19834913A DE19834913A1 (de) | 1998-08-03 | 1998-08-03 | Markierter Primer zum Nachweis amplifizierter Nukleinsäuren |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE369439T1 true ATE369439T1 (de) | 2007-08-15 |
Family
ID=27218019
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT98123569T ATE369439T1 (de) | 1997-12-15 | 1998-12-10 | Markierter primer, geeignet für die detektion von nukleinsäuren |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US6248526B1 (de) |
| EP (1) | EP0930370B1 (de) |
| JP (1) | JP4331812B2 (de) |
| KR (1) | KR100702338B1 (de) |
| AT (1) | ATE369439T1 (de) |
| AU (1) | AU741053B2 (de) |
| CA (1) | CA2255531C (de) |
| DE (1) | DE69838210T2 (de) |
| ES (1) | ES2290979T3 (de) |
Families Citing this family (65)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020188268A1 (en) * | 1999-06-30 | 2002-12-12 | Mark James Kline | Elastomeric side panel for use with convertible absorbent articles |
| WO2000079009A2 (en) | 1999-06-22 | 2000-12-28 | Invitrogen Corporation | Improved primers and methods for the detection and discrimination of nucleic acids |
| US20070134686A1 (en) * | 1999-10-29 | 2007-06-14 | Stratagene California | Methods and compositions for detection of a target nucleic acid sequence utilizing a probe with a 3' flap |
| WO2001048246A1 (en) * | 1999-12-29 | 2001-07-05 | Vanderbilt University | Genetic mutation underlying orthostatic intolerance and diagnostic and therapeutic methods relating thereto |
| FI20000333A0 (fi) | 2000-02-16 | 2000-02-16 | Jussi Nurmi | Homogeeninen menetelmä polynukleotidin havaitsemiseksi |
| US7198924B2 (en) | 2000-12-11 | 2007-04-03 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
| WO2002057479A2 (en) * | 2000-12-27 | 2002-07-25 | Invitrogen Corporation | Primers and methods for the detection and discrimination of nucleic acids |
| KR20020089746A (ko) * | 2001-05-24 | 2002-11-30 | 주식회사 커벡스 | 크기변형유도 앵커 시발체, 이를 이용한 알티-피씨알 방법및 이를 이용한 진단 방법 |
| WO2003008643A2 (en) * | 2001-07-20 | 2003-01-30 | Cancer Research Technology Limited | Methods and polynukleotides for assaying the activity of a dna modifying enzyme |
| FR2829148B1 (fr) * | 2001-09-06 | 2004-10-15 | Univ Aix Marseille Ii | Identification moleculaire des bacteries du genre staphylococcus |
| US20030165859A1 (en) * | 2001-10-23 | 2003-09-04 | Invitrogen Corporation | Primers and methods for the detection and discrimination of nucleic acids |
| DE60235491D1 (de) * | 2001-11-28 | 2010-04-08 | Bio Rad Laboratories | Paralleles scoring von polymorphismen mittels amplifikation und fehlerkorrektur |
| GB0200526D0 (en) * | 2002-01-11 | 2002-02-27 | Science And Technology The | Methods |
| JP4481656B2 (ja) * | 2002-02-27 | 2010-06-16 | ライフ テクノロジーズ コーポレーション | 3’→5’エキソヌクレアーゼ耐性クエンチャードメインを含むfet標識オリゴヌクレオチドを使用する方法およびそれを実施するための組成物 |
| US6818420B2 (en) * | 2002-02-27 | 2004-11-16 | Biosource International, Inc. | Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same |
| CN1414110A (zh) * | 2002-08-19 | 2003-04-30 | 张旭 | 利用具有3'至5'外切酶活性的多聚酶进行基因序列分析 |
| EP1611254B1 (de) | 2003-03-31 | 2014-09-10 | Roche Diagnostics GmbH | Zusammensetzungen und verfahren zum nachweis bestimmter flaviviren, einschliesslich mitgliedern der japanese-encephalitis-virus-serogruppe |
| EP1502958A1 (de) * | 2003-08-01 | 2005-02-02 | Roche Diagnostics GmbH | Neues Format zur Detektion von Heissstart- und Echtzeit-Polymerasekettenreaktion |
| WO2005054438A2 (en) | 2003-12-01 | 2005-06-16 | Invitrogen Corporation | Nucleic acid molecules containing recombination sites and methods of using the same |
| GB0406015D0 (en) * | 2004-03-17 | 2004-04-21 | Dynal Biotech Asa | Improvements in magnetic polymer particles |
| US7445898B2 (en) | 2004-04-01 | 2008-11-04 | Bio-Rad Laboratories, Inc. | Quantitative amplification with a labeled probe and 3′ to 5′ exonuclease activity |
| CA2502549C (en) * | 2004-04-23 | 2016-02-16 | Becton, Dickinson And Company | Use of an extraction control in a method of extracting nucleic acids |
| US20060105348A1 (en) * | 2004-11-15 | 2006-05-18 | Lee Jun E | Compositions and methods for the detection and discrimination of nucleic acids |
| US20060275792A1 (en) * | 2004-11-15 | 2006-12-07 | Lee Jun E | Enhancement of nucleic acid amplification using double-stranded DNA binding proteins |
| EP1892302A1 (de) * | 2005-06-16 | 2008-02-27 | BIOTOOLS BIOTECHNOLOGICAL & MEDICAL LABORATORIES, S.A. | Nukleinsäurenachweisverfahren mit direkter erzeugung eines messbaren signals |
| DK2302070T3 (da) | 2005-06-23 | 2012-11-26 | Keygene Nv | Strategier til identifikation og detektion af polymorfismer med højt gennemløb |
| US10316364B2 (en) | 2005-09-29 | 2019-06-11 | Keygene N.V. | Method for identifying the source of an amplicon |
| WO2007037678A2 (en) | 2005-09-29 | 2007-04-05 | Keygene N.V. | High throughput screening of mutagenized populations |
| US7781165B2 (en) | 2005-10-19 | 2010-08-24 | Roche Diagnostics Operations, Inc. | Benzimidazolium compounds and salts of benzimidazolium compounds for nucleic acid amplification |
| CN103937899B (zh) | 2005-12-22 | 2017-09-08 | 凯津公司 | 用于基于aflp的高通量多态性检测的方法 |
| CN101421417B (zh) | 2006-02-27 | 2013-04-03 | 霍夫曼-拉罗奇有限公司 | 经由镁螯合的pcr热启动 |
| GB0605584D0 (en) * | 2006-03-20 | 2006-04-26 | Olink Ab | Method for analyte detection using proximity probes |
| EP3239304B1 (de) | 2006-04-04 | 2020-08-19 | Keygene N.V. | Nachweis von molekularmarkern mit hohem durchsatz auf der basis von aflp und mit hoher durchsatzsequenzierung |
| EP2284262A4 (de) * | 2008-05-28 | 2012-02-22 | Wako Pure Chem Ind Ltd | Primer und sonde für den nachweis von mycobacterium intracellulare und verfahren für den nachweis von mycobacterium intracellulare mit dem primer oder der sonde |
| US9447457B2 (en) * | 2009-09-24 | 2016-09-20 | Seegene, Inc. | Detection of target nucleic acid sequences by cyclic exonucleolytic reactions |
| WO2011056687A2 (en) * | 2009-10-27 | 2011-05-12 | Swift Biosciences, Inc. | Polynucleotide primers and probes |
| KR20110050327A (ko) | 2009-11-07 | 2011-05-13 | 주식회사 씨젠 | Thd 프라이머 타겟 검출 |
| JP5822843B2 (ja) * | 2009-12-21 | 2015-11-24 | シージーン アイエヌシー | Tsgプライマーターゲット検出 |
| GB201004292D0 (en) * | 2010-03-15 | 2010-04-28 | Olink Ab | Assay for localised detection of analytes |
| GB201011971D0 (en) | 2010-07-15 | 2010-09-01 | Olink Ab | Methods and product |
| US10669569B2 (en) | 2010-10-15 | 2020-06-02 | Navinci Diagnostics Ab | Dynamic range methods |
| GB201101621D0 (en) | 2011-01-31 | 2011-03-16 | Olink Ab | Method and product |
| GB201107863D0 (en) | 2011-05-11 | 2011-06-22 | Olink Ab | Method and product |
| EP2559774A1 (de) | 2011-08-17 | 2013-02-20 | Roche Diagniostics GmbH | Verbessertes Verfahren zur Amplifikation von Target-Nucleinsäuren mithilfe eines Multi-Primer-Ansatzes |
| GB201201547D0 (en) | 2012-01-30 | 2012-03-14 | Olink Ab | Method and product |
| US20150232940A1 (en) * | 2012-03-09 | 2015-08-20 | Insight Genetics, Inc | Methods and compositions relating to diagnosing and treating receptor tyrosine kinase related cancers |
| EP2920324B1 (de) | 2012-11-14 | 2017-12-27 | Olink Bioscience AB | Lokalisiertes rca-basiertes verstärkungsverfahren |
| US20160369321A1 (en) | 2012-11-14 | 2016-12-22 | Olink Ab | RCA Reporter Probes and Their Use in Detecting Nucleic Acid Molecules |
| US20140341884A1 (en) * | 2012-12-04 | 2014-11-20 | Roche Molecular Systems, Inc. | Novel Complex Mutations in the Epidermal Growth Factor Receptor Kinase Domain |
| EP4570924A3 (de) | 2013-01-24 | 2025-09-03 | California Institute of Technology | Chromophorbasierte charakterisierungs- und nachweisverfahren |
| WO2014165753A1 (en) * | 2013-04-05 | 2014-10-09 | The Wistar Institute Of Anatomy And Biology | Methods and compositions for diagnosis of glioblastoma or a subtype thereof |
| GB201320145D0 (en) | 2013-11-14 | 2014-01-01 | Olink Ab | Localised RCA-based amplification method using a padlock-probe |
| US20150153346A1 (en) * | 2013-11-15 | 2015-06-04 | The Regents Of The University Of Michigan | Lung cancer signature |
| GB201401885D0 (en) | 2014-02-04 | 2014-03-19 | Olink Ab | Proximity assay with detection based on hybridisation chain reaction (HCR) |
| CN107109497A (zh) * | 2014-12-31 | 2017-08-29 | 豪夫迈·罗氏有限公司 | 通过实时PCR从细胞裂解物定量HBV cccDNA的高通量新方法 |
| GB201518655D0 (en) | 2015-10-21 | 2015-12-02 | Olink Ab | Method for generating proximity probes |
| US11279980B2 (en) * | 2016-01-25 | 2022-03-22 | University Of Utah Research Foundation | Methods and compositions for predicting a colon cancer subtype |
| GB201621514D0 (en) | 2016-12-16 | 2017-02-01 | Q-Linea Ab | Padlock probe detection method |
| CN120210336A (zh) | 2017-10-06 | 2025-06-27 | 10X基因组学有限公司 | Rna模板化连接 |
| US20190112636A1 (en) * | 2017-10-16 | 2019-04-18 | ChromaCode, Inc. | Methods and compositions for nucleic acid detection |
| US12454720B2 (en) | 2018-04-17 | 2025-10-28 | ChromaCode, Inc. | Methods and systems for multiplex analysis |
| US12203129B2 (en) | 2018-07-03 | 2025-01-21 | ChromaCode, Inc. | Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays |
| CN109694905A (zh) * | 2019-01-29 | 2019-04-30 | 深圳市亚辉龙生物科技股份有限公司 | 检测待测基因甲基化的核酸组合物及其试剂盒和待测基因甲基化的检测方法 |
| GB201919029D0 (en) | 2019-12-20 | 2020-02-05 | Cartana Ab | Method of detecting an analyte |
| GB201919032D0 (en) | 2019-12-20 | 2020-02-05 | Cartana Ab | Method of detecting an analyte |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU5640090A (en) | 1989-03-21 | 1990-11-05 | Collaborative Research Inc. | A dna diagnostic test using an exonuclease activity |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| US5804380A (en) * | 1993-11-12 | 1998-09-08 | Geron Corporation | Telomerase activity assays |
| US5716784A (en) * | 1996-02-05 | 1998-02-10 | The Perkin-Elmer Corporation | Fluorescence detection assay for homogeneous PCR hybridization systems |
-
1998
- 1998-12-10 EP EP98123569A patent/EP0930370B1/de not_active Expired - Lifetime
- 1998-12-10 AT AT98123569T patent/ATE369439T1/de active
- 1998-12-10 DE DE69838210T patent/DE69838210T2/de not_active Expired - Lifetime
- 1998-12-10 ES ES98123569T patent/ES2290979T3/es not_active Expired - Lifetime
- 1998-12-14 US US09/210,657 patent/US6248526B1/en not_active Expired - Lifetime
- 1998-12-14 AU AU97088/98A patent/AU741053B2/en not_active Ceased
- 1998-12-14 CA CA002255531A patent/CA2255531C/en not_active Expired - Fee Related
- 1998-12-15 KR KR1019980054960A patent/KR100702338B1/ko not_active Expired - Fee Related
- 1998-12-15 JP JP35573398A patent/JP4331812B2/ja not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP0930370A1 (de) | 1999-07-21 |
| JP4331812B2 (ja) | 2009-09-16 |
| ES2290979T3 (es) | 2008-02-16 |
| KR100702338B1 (ko) | 2008-03-19 |
| US6248526B1 (en) | 2001-06-19 |
| AU741053B2 (en) | 2001-11-22 |
| CA2255531C (en) | 2007-11-13 |
| EP0930370B1 (de) | 2007-08-08 |
| AU9708898A (en) | 1999-07-01 |
| KR19990063053A (ko) | 1999-07-26 |
| JPH11313685A (ja) | 1999-11-16 |
| CA2255531A1 (en) | 1999-06-15 |
| DE69838210D1 (de) | 2007-09-20 |
| DE69838210T2 (de) | 2008-05-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |
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