ATE371027T1 - Expressions- und sekretionsvektor für menschliches interferon alpha und verfahren zur herstellung von interferon alpha durch anwendung desselben - Google Patents

Expressions- und sekretionsvektor für menschliches interferon alpha und verfahren zur herstellung von interferon alpha durch anwendung desselben

Info

Publication number
ATE371027T1
ATE371027T1 AT01949005T AT01949005T ATE371027T1 AT E371027 T1 ATE371027 T1 AT E371027T1 AT 01949005 T AT01949005 T AT 01949005T AT 01949005 T AT01949005 T AT 01949005T AT E371027 T1 ATE371027 T1 AT E371027T1
Authority
AT
Austria
Prior art keywords
interferon alpha
human interferon
hifnalpha
same
producing
Prior art date
Application number
AT01949005T
Other languages
English (en)
Inventor
Se Kwon
Sung Youb Jung
Ki Doo Choi
Cha Soon Kim
Sung Bae
Gwan Sun Lee
Original Assignee
Hanmi Pharm Ind Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hanmi Pharm Ind Co Ltd filed Critical Hanmi Pharm Ind Co Ltd
Application granted granted Critical
Publication of ATE371027T1 publication Critical patent/ATE371027T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/56—IFN-alpha
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AT01949005T 2000-01-19 2001-01-19 Expressions- und sekretionsvektor für menschliches interferon alpha und verfahren zur herstellung von interferon alpha durch anwendung desselben ATE371027T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1020000002434A KR100360594B1 (ko) 2000-01-19 2000-01-19 인간 인터페론 알파의 발현 분비벡터 및 이를 이용한인터페론 알파의 생산 방법

Publications (1)

Publication Number Publication Date
ATE371027T1 true ATE371027T1 (de) 2007-09-15

Family

ID=19639684

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01949005T ATE371027T1 (de) 2000-01-19 2001-01-19 Expressions- und sekretionsvektor für menschliches interferon alpha und verfahren zur herstellung von interferon alpha durch anwendung desselben

Country Status (14)

Country Link
US (1) US7052867B2 (de)
EP (1) EP1250439B1 (de)
JP (1) JP3751883B2 (de)
KR (1) KR100360594B1 (de)
CN (1) CN100436581C (de)
AT (1) ATE371027T1 (de)
AU (1) AU762053B2 (de)
BR (1) BRPI0107708A8 (de)
CA (1) CA2397527C (de)
DE (1) DE60130100T2 (de)
ES (1) ES2290156T3 (de)
NZ (1) NZ520076A (de)
RU (1) RU2230116C2 (de)
WO (1) WO2001057217A1 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SI1656455T1 (sl) * 2003-08-13 2012-12-31 Sandoz Ag Postopek za čiščenje rekombinantnih polipeptidov
DE602004004796T2 (de) 2003-08-13 2007-12-06 Sandoz Ag Expressionsvektoren, transformierte wirtszellen und fermentationsverfahren zur herstellung rekombinanter polypeptide
GB0319601D0 (en) * 2003-08-20 2003-09-24 Sandoz Ag Production process
ES2383300T3 (es) 2003-11-13 2012-06-20 Hanmi Holdings Co., Ltd Fragmento Fc de IgG para un vehículo de fármacos y procedimiento para su preparación
US8110665B2 (en) 2003-11-13 2012-02-07 Hanmi Holdings Co., Ltd. Pharmaceutical composition comprising an immunoglobulin FC region as a carrier
RU2303063C2 (ru) * 2005-09-29 2007-07-20 ООО "Цитокин" ШТАММ Escherichia coli BL21 (DE3)[pAYC-ET-(hIFN-α2b)-IacI]-ПРОДУЦЕНТ РЕКОМБИНАНТНОГО ЧЕЛОВЕЧЕСКОГО АЛЬФА-2b ИНТЕРФЕРОНА И СПОСОБ ЕГО КУЛЬТИВИРОВАНИЯ
WO2009046080A1 (en) 2007-10-01 2009-04-09 Pharmaessentia Corp. N-terminal modified interferon-alpha
FR2964434B1 (fr) 2010-09-07 2012-08-24 Walden Associates Ltd S A Amortisseur a haut pouvoir dissipatif et pratiquement sans huile
CN111053918A (zh) 2011-09-05 2020-04-24 韩美科学株式会社 一种包含干扰素α-缀合物的抗癌用药物组合物
US11471497B1 (en) 2019-03-13 2022-10-18 David Gordon Bermudes Copper chelation therapeutics
US10973908B1 (en) 2020-05-14 2021-04-13 David Gordon Bermudes Expression of SARS-CoV-2 spike protein receptor binding domain in attenuated salmonella as a vaccine
US12537071B1 (en) 2020-07-22 2026-01-27 David Gordon Bermudes Bacteria having boolean control pathways expressing therapeutic proteins including immunotherapeutic cytotoxins

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0177343B1 (de) * 1984-10-05 1992-07-22 Genentech, Inc. DNA, Zellkulturen und Verfahren zur Sekretion von heterologen Proteinen und periplasmische Proteinrückgewinnung
JPS63230089A (ja) 1987-03-18 1988-09-26 Takeda Chem Ind Ltd 分泌発現による蛋白質の製造法
EP0626448A3 (de) * 1993-05-26 1998-01-14 BOEHRINGER INGELHEIM INTERNATIONAL GmbH Verfahren zur Herstellung und Reinigung von alpha-Interferon
US5470719A (en) 1994-03-18 1995-11-28 Meng; Shi-Yuan Modified OmpA signal sequence for enhanced secretion of polypeptides
RU2097428C1 (ru) * 1996-12-23 1997-11-27 Государственный научный центр прикладной микробиологии Рекомбинантная плазмидная днк рттg кm2, кодирующая синтез рекомбинантного иммунного интерферона человека, штамм escherichia coli t3g - продуцент рекомбинантного иммунного интерферона человека и способ получения рекомбинантного иммунного интерферона человека
KR100316347B1 (ko) * 1998-09-15 2002-08-27 한미약품(주) 대장균엔테로톡신ⅱ신호펩티드의변형체와인체성장호르몬의융합단백질을발현하는재조합미생물및그를이용한인체성장호르몬의제조방법

Also Published As

Publication number Publication date
WO2001057217A9 (en) 2002-12-27
EP1250439B1 (de) 2007-08-22
BR0107708A (pt) 2002-11-19
KR100360594B1 (ko) 2002-11-13
JP3751883B2 (ja) 2006-03-01
BRPI0107708A8 (pt) 2016-06-07
AU2890901A (en) 2001-08-14
NZ520076A (en) 2003-07-25
CN100436581C (zh) 2008-11-26
KR20010073658A (ko) 2001-08-01
RU2230116C2 (ru) 2004-06-10
EP1250439A4 (de) 2004-04-07
WO2001057217A1 (en) 2001-08-09
US7052867B2 (en) 2006-05-30
ES2290156T3 (es) 2008-02-16
DE60130100T2 (de) 2008-05-15
AU762053B2 (en) 2003-06-19
CA2397527A1 (en) 2001-08-09
JP2003521925A (ja) 2003-07-22
EP1250439A1 (de) 2002-10-23
US20040151695A1 (en) 2004-08-05
CA2397527C (en) 2010-10-19
CN1418251A (zh) 2003-05-14
DE60130100D1 (de) 2007-10-04

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