ATE455125T1 - Tool für den transfer und die herstelung von proteinen mit dem pseudomonas-typ-iii- sekretionssystem - Google Patents

Tool für den transfer und die herstelung von proteinen mit dem pseudomonas-typ-iii- sekretionssystem

Info

Publication number
ATE455125T1
ATE455125T1 AT04805807T AT04805807T ATE455125T1 AT E455125 T1 ATE455125 T1 AT E455125T1 AT 04805807 T AT04805807 T AT 04805807T AT 04805807 T AT04805807 T AT 04805807T AT E455125 T1 ATE455125 T1 AT E455125T1
Authority
AT
Austria
Prior art keywords
terminus
sequence
exos
proteins
tool
Prior art date
Application number
AT04805807T
Other languages
English (en)
Inventor
Benoit Polack
Bertrand Toussaint
Lauriane Quenee
Original Assignee
Univ Joseph Fourier
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Joseph Fourier filed Critical Univ Joseph Fourier
Application granted granted Critical
Publication of ATE455125T1 publication Critical patent/ATE455125T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/78—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/21—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pseudomonadaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT04805807T 2003-11-13 2004-11-04 Tool für den transfer und die herstelung von proteinen mit dem pseudomonas-typ-iii- sekretionssystem ATE455125T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0313286A FR2862312B1 (fr) 2003-11-13 2003-11-13 Outil de transfert et de production de proteines mettant en oeuvre le systeme de secretion de type iii de pseudomonas
PCT/FR2004/050564 WO2005049644A2 (fr) 2003-11-13 2004-11-04 Outil de transfert et de production de proteines mettant en oeuvre le systeme de secretion de type iii de pseudomonas

Publications (1)

Publication Number Publication Date
ATE455125T1 true ATE455125T1 (de) 2010-01-15

Family

ID=34508435

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04805807T ATE455125T1 (de) 2003-11-13 2004-11-04 Tool für den transfer und die herstelung von proteinen mit dem pseudomonas-typ-iii- sekretionssystem

Country Status (7)

Country Link
US (1) US7939319B2 (de)
EP (1) EP1692162B1 (de)
AT (1) ATE455125T1 (de)
DE (1) DE602004025139D1 (de)
ES (1) ES2339118T3 (de)
FR (1) FR2862312B1 (de)
WO (1) WO2005049644A2 (de)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080124355A1 (en) 2006-09-22 2008-05-29 David Gordon Bermudes Live bacterial vaccines for viral infection prophylaxis or treatment
ES2331271B1 (es) * 2007-06-29 2010-10-14 Universidad Del Pais Vasco Metodo para la internalizacion de bacterias no invasivas en celulas eucariotas.
US8617888B2 (en) * 2010-07-23 2013-12-31 University Of Florida Research Foundation, Inc. Bacterial mediated delivery of nuclear protein into pluripotent and differentiated cells
EP2604682A1 (de) 2011-12-12 2013-06-19 Universite Joseph Fourier - Grenoble 1 Abgeschwächter und angepasster Stamm aus Pseudomonen zur Lieferung von Antigenen
WO2014150179A1 (en) * 2013-03-15 2014-09-25 Rush University Medical Center Pseudomonas exotoxins for cancer treatment
BR112016027196B1 (pt) * 2014-05-21 2023-05-02 Universität Basel Vetor e cepa bacteriana gram-negativa recombinante
US9616114B1 (en) 2014-09-18 2017-04-11 David Gordon Bermudes Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
EP3101134A1 (de) 2015-06-05 2016-12-07 Apcure SAS Therapeutischer impfstoff zur behandlung oder prävention von tumoren im zusammenhang mit merkelzell-polyomavirus
SG11201803643TA (en) 2015-11-19 2018-06-28 Univ Basel Virulence attenuated bacteria for treatment of malignant solid tumors
HUE051184T2 (hu) * 2015-11-19 2021-03-01 Univ Basel Baktériumalapú fehérjeszállítás
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
EA201991157A1 (ru) 2016-12-20 2020-01-16 Университет Базель Доставка белков на основе бактерий, аттенуированных по вирулентности
EP3363460A1 (de) 2017-02-16 2018-08-22 Université Grenoble Alpes Abgeschwächter stamm von pseudomonas als impfstoff gegen pseudomonas-infektionen
US11471497B1 (en) 2019-03-13 2022-10-18 David Gordon Bermudes Copper chelation therapeutics
CN110922454B (zh) * 2019-12-05 2020-11-06 中国科学院深圳先进技术研究院 绿脓杆菌毒素ExoS和ExoT的免疫用途及其制备方法
US10973908B1 (en) 2020-05-14 2021-04-13 David Gordon Bermudes Expression of SARS-CoV-2 spike protein receptor binding domain in attenuated salmonella as a vaccine
US12537071B1 (en) 2020-07-22 2026-01-27 David Gordon Bermudes Bacteria having boolean control pathways expressing therapeutic proteins including immunotherapeutic cytotoxins
CN112237623B (zh) * 2020-10-30 2023-10-03 中国人民解放军陆军军医大学第二附属医院 铜绿假单胞菌III型分泌型蛋白PcrV及其诱导极化的巨噬细胞的应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6306387B1 (en) 1997-05-29 2001-10-23 The Research Foundation Of State University Of New York Antigen delivery system
US5965381A (en) 1998-03-06 1999-10-12 Ludwig Institute For Cancer Research Delivery of proteins into eukaryotic cells with recombinant yersinia

Also Published As

Publication number Publication date
WO2005049644A3 (fr) 2005-11-17
EP1692162B1 (de) 2010-01-13
ES2339118T3 (es) 2010-05-17
DE602004025139D1 (de) 2010-03-04
FR2862312B1 (fr) 2006-02-17
WO2005049644A2 (fr) 2005-06-02
FR2862312A1 (fr) 2005-05-20
US20080187520A1 (en) 2008-08-07
EP1692162A2 (de) 2006-08-23
US7939319B2 (en) 2011-05-10

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