ATE455125T1 - Tool für den transfer und die herstelung von proteinen mit dem pseudomonas-typ-iii- sekretionssystem - Google Patents

Tool für den transfer und die herstelung von proteinen mit dem pseudomonas-typ-iii- sekretionssystem

Info

Publication number
ATE455125T1
ATE455125T1 AT04805807T AT04805807T ATE455125T1 AT E455125 T1 ATE455125 T1 AT E455125T1 AT 04805807 T AT04805807 T AT 04805807T AT 04805807 T AT04805807 T AT 04805807T AT E455125 T1 ATE455125 T1 AT E455125T1
Authority
AT
Austria
Prior art keywords
terminus
sequence
exos
proteins
tool
Prior art date
Application number
AT04805807T
Other languages
English (en)
Inventor
Benoit Polack
Bertrand Toussaint
Lauriane Quenee
Original Assignee
Univ Joseph Fourier
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Joseph Fourier filed Critical Univ Joseph Fourier
Application granted granted Critical
Publication of ATE455125T1 publication Critical patent/ATE455125T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/78—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/21—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pseudomonadaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT04805807T 2003-11-13 2004-11-04 Tool für den transfer und die herstelung von proteinen mit dem pseudomonas-typ-iii- sekretionssystem ATE455125T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR0313286A FR2862312B1 (fr) 2003-11-13 2003-11-13 Outil de transfert et de production de proteines mettant en oeuvre le systeme de secretion de type iii de pseudomonas
PCT/FR2004/050564 WO2005049644A2 (fr) 2003-11-13 2004-11-04 Outil de transfert et de production de proteines mettant en oeuvre le systeme de secretion de type iii de pseudomonas

Publications (1)

Publication Number Publication Date
ATE455125T1 true ATE455125T1 (de) 2010-01-15

Family

ID=34508435

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04805807T ATE455125T1 (de) 2003-11-13 2004-11-04 Tool für den transfer und die herstelung von proteinen mit dem pseudomonas-typ-iii- sekretionssystem

Country Status (7)

Country Link
US (1) US7939319B2 (de)
EP (1) EP1692162B1 (de)
AT (1) ATE455125T1 (de)
DE (1) DE602004025139D1 (de)
ES (1) ES2339118T3 (de)
FR (1) FR2862312B1 (de)
WO (1) WO2005049644A2 (de)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080124355A1 (en) 2006-09-22 2008-05-29 David Gordon Bermudes Live bacterial vaccines for viral infection prophylaxis or treatment
ES2331271B1 (es) * 2007-06-29 2010-10-14 Universidad Del Pais Vasco Metodo para la internalizacion de bacterias no invasivas en celulas eucariotas.
US8617888B2 (en) * 2010-07-23 2013-12-31 University Of Florida Research Foundation, Inc. Bacterial mediated delivery of nuclear protein into pluripotent and differentiated cells
EP2604682A1 (de) 2011-12-12 2013-06-19 Universite Joseph Fourier - Grenoble 1 Abgeschwächter und angepasster Stamm aus Pseudomonen zur Lieferung von Antigenen
WO2014150179A1 (en) * 2013-03-15 2014-09-25 Rush University Medical Center Pseudomonas exotoxins for cancer treatment
CN107001431B (zh) * 2014-05-21 2022-11-01 巴塞尔大学 基于细菌的蛋白质递送
US9616114B1 (en) 2014-09-18 2017-04-11 David Gordon Bermudes Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
EP3101134A1 (de) 2015-06-05 2016-12-07 Apcure SAS Therapeutischer impfstoff zur behandlung oder prävention von tumoren im zusammenhang mit merkelzell-polyomavirus
SMT202000620T1 (it) * 2015-11-19 2021-01-05 Univ Basel Distribuzione di proteine basata su batteri
EA201890868A1 (ru) 2015-11-19 2018-12-28 Университет Базель Бактерии с ослабленной вирулентностью для лечения злокачественных солидных опухолей
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
JP6976351B2 (ja) 2016-12-20 2021-12-08 ウニヴェルズィテート・バーゼルUniversitat Basel 弱毒化細菌に基づくタンパク質送達
EP3363460A1 (de) 2017-02-16 2018-08-22 Université Grenoble Alpes Abgeschwächter stamm von pseudomonas als impfstoff gegen pseudomonas-infektionen
US11471497B1 (en) 2019-03-13 2022-10-18 David Gordon Bermudes Copper chelation therapeutics
CN110922454B (zh) * 2019-12-05 2020-11-06 中国科学院深圳先进技术研究院 绿脓杆菌毒素ExoS和ExoT的免疫用途及其制备方法
US10973908B1 (en) 2020-05-14 2021-04-13 David Gordon Bermudes Expression of SARS-CoV-2 spike protein receptor binding domain in attenuated salmonella as a vaccine
US12537071B1 (en) 2020-07-22 2026-01-27 David Gordon Bermudes Bacteria having boolean control pathways expressing therapeutic proteins including immunotherapeutic cytotoxins
CN112237623B (zh) * 2020-10-30 2023-10-03 中国人民解放军陆军军医大学第二附属医院 铜绿假单胞菌III型分泌型蛋白PcrV及其诱导极化的巨噬细胞的应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6306387B1 (en) 1997-05-29 2001-10-23 The Research Foundation Of State University Of New York Antigen delivery system
US5965381A (en) 1998-03-06 1999-10-12 Ludwig Institute For Cancer Research Delivery of proteins into eukaryotic cells with recombinant yersinia

Also Published As

Publication number Publication date
WO2005049644A2 (fr) 2005-06-02
FR2862312A1 (fr) 2005-05-20
US20080187520A1 (en) 2008-08-07
ES2339118T3 (es) 2010-05-17
US7939319B2 (en) 2011-05-10
EP1692162B1 (de) 2010-01-13
EP1692162A2 (de) 2006-08-23
WO2005049644A3 (fr) 2005-11-17
DE602004025139D1 (de) 2010-03-04
FR2862312B1 (fr) 2006-02-17

Similar Documents

Publication Publication Date Title
DE602004025139D1 (de) Tool für den transfer und die herstelung von proteinen mit dem pseudomonas-typ-iii-sekretionssystem
US11214609B2 (en) Recombinant collagen and elastin molecules and uses thereof
DE69634640D1 (de) Mutierte alpha-amylase enzyme mit erhöhter stabilität
Brown et al. Expression in bacteria of functional inhibitory subunit of retinal rod cGMP phosphodiesterase.
AU6860396A (en) Purified coprinus laccases and nucleic acids encoding same
DE60033138D1 (de) Polypeptide mit galaktoseoxidaseaktivität und dafür kodierende nukleisäuren
CA2400295A1 (en) Modified factor viii
WO1992006211A1 (en) Process for producing peptide or protein
Baramova et al. Identification of the cleavage sites by a hemorrhagic metalloproteinase in type IV collagen
CN101250547A (zh) 人成纤维细胞生长因子-21的重组表达
MX2025009545A (es) Metodos y composiciones para la administracion de sistemas de vector dual otoferlin
ATE450603T1 (de) Polypeptide mit verzweigungsenzym-aktivität und für diese kodierende nukleinsäuren
DK1792982T3 (da) Codon-optimeret phosphatase og ekspression heraf i gær
BR9802403A (pt) Proteìna tendo atividade transglutaminase, dna, transformante, e, processo para produzir uma proteìna tendo uma atividade transglutaminase.
PE20020857A1 (es) Proteinas de fusion cuya parte del n-terminal es un derivado de hirudina para la produccion de proteinas recombinantes a traves de la secresion por levaduras
EP0422049A4 (en) Vector for secretion of proteins directly into periplasm or culture medium
ATE334195T1 (de) Proteine mit zellulase-aktivitäten und prozess für ihre herstellung
EP0528686B8 (de) Verfahren zur Herstellung von Peptiden
WO2005001099A3 (en) Protein expression system
Ruoppolo et al. Slow folding of three-fingered toxins is associated with the accumulation of native disulfide-bonded intermediates
ATE202798T1 (de) D-aminosäure-oxidase kodierendes dns-fragment
EP2177606A3 (de) N-Desoxyribosyltransferasen von Laktobazillen, entsprechende Nukleotidsequenzen und ihre Anwendungen
NO326247B1 (no) Fremgangsmate for fremstilling av modent insulin eller et modent insulin derivat.
DK0828004T3 (da) Proteindisulfidisomerase-gen afledt af stamme af methylotrof gær
SE0303093D0 (sv) Expression vector, host cell and method for producing fusion proteins

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties