ATE374822T1 - Mutanter apre promoter - Google Patents

Mutanter apre promoter

Info

Publication number
ATE374822T1
ATE374822T1 AT00990354T AT00990354T ATE374822T1 AT E374822 T1 ATE374822 T1 AT E374822T1 AT 00990354 T AT00990354 T AT 00990354T AT 00990354 T AT00990354 T AT 00990354T AT E374822 T1 ATE374822 T1 AT E374822T1
Authority
AT
Austria
Prior art keywords
mutant
apre promoter
promoter
mutant apre
apre
Prior art date
Application number
AT00990354T
Other languages
English (en)
Inventor
Eugenio Ferrari
Fernando Valle
Original Assignee
Genencor Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Int filed Critical Genencor Int
Application granted granted Critical
Publication of ATE374822T1 publication Critical patent/ATE374822T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
AT00990354T 2000-01-07 2000-12-21 Mutanter apre promoter ATE374822T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/479,494 US6509185B1 (en) 2000-01-07 2000-01-07 Mutant aprE promotor

Publications (1)

Publication Number Publication Date
ATE374822T1 true ATE374822T1 (de) 2007-10-15

Family

ID=23904251

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00990354T ATE374822T1 (de) 2000-01-07 2000-12-21 Mutanter apre promoter

Country Status (12)

Country Link
US (2) US6509185B1 (de)
EP (1) EP1244794B1 (de)
JP (1) JP4870306B2 (de)
KR (1) KR20020073497A (de)
CN (2) CN1313615C (de)
AT (1) ATE374822T1 (de)
AU (1) AU2739001A (de)
CA (1) CA2396250C (de)
DE (1) DE60036641T2 (de)
DK (1) DK1244794T3 (de)
MX (1) MXPA02006614A (de)
WO (1) WO2001051643A1 (de)

Families Citing this family (47)

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US6509185B1 (en) * 2000-01-07 2003-01-21 Genencor International, Inc. Mutant aprE promotor
ATE495245T1 (de) * 2002-02-15 2011-01-15 Danisco Us Inc Verbesserte proteinexpression in bacillus subtilis
DK1495128T3 (da) * 2002-03-29 2014-08-11 Genencor Int Forstærket proteinekspression i Bacillus
DK2152732T3 (da) 2007-05-10 2012-06-04 Danisco Us Inc Modificeret sekretionssystem til at øge ekspression af polypeptider i bakterier
US20090048136A1 (en) * 2007-08-15 2009-02-19 Mcdonald Hugh C Kappa-carrageenase and kappa-carrageenase-containing compositions
DK2195435T3 (da) 2007-09-12 2012-12-10 Danisco Us Inc Trichoderma-promoter
AU2008348270A1 (en) 2007-12-21 2009-07-30 Danisco Us Inc. Enhanced protein production in bacillus
AR076941A1 (es) 2009-06-11 2011-07-20 Danisco Us Inc Cepa de bacillus para una mayor produccion de proteina
WO2013086219A1 (en) 2011-12-09 2013-06-13 Danisco Us Inc. Ribosomal promotors from b. subtilis for protein production in microorganisms
KR102588719B1 (ko) * 2014-12-16 2023-10-12 다니스코 유에스 인크. 향상된 단백질 발현
JP7025325B2 (ja) 2015-10-30 2022-02-24 ダニスコ・ユーエス・インク タンパク質発現の増強およびその方法
EP3380496B1 (de) 2015-12-23 2020-05-20 Danisco US Inc. Erhöhte proteinproduktion und verfahren dafür
CN109071615A (zh) 2016-03-04 2018-12-21 丹尼斯科美国公司 用于在微生物中产生蛋白质的工程化核糖体启动子
DK3558026T3 (da) 2016-12-21 2026-01-12 Int N&H Denmark Aps Fremgangsmåder til anvendelse af termostabile serinproteaser
US20190367932A1 (en) 2017-01-23 2019-12-05 Danisco Us Inc. Methods and compositions for obtaining natural comptence in bacillus host cells
JP7231228B2 (ja) 2017-02-24 2023-03-01 ダニスコ・ユーエス・インク バチルス・リケニフォルミスにおける増加したタンパク質産生のための組成物及び方法
BR112019018969A2 (pt) 2017-03-15 2020-04-22 Dupont Nutrition Biosci Aps serina proteases similar à tripsina e usos das mesmas
US11453871B2 (en) 2017-03-15 2022-09-27 Danisco Us Inc. Trypsin-like serine proteases and uses thereof
EP3596211B1 (de) 2017-03-15 2021-06-02 DuPont Nutrition Biosciences ApS Verwendungen einer archaealen serinprotease
DK3607097T3 (da) 2017-04-07 2023-09-18 Dupont Nutrition Biosci Aps Bacillus-værtsceller, der danner beta-galactosidaser og lactaser i fravær af p-nitrobenzylesterase-sideaktivitet
CN107142259A (zh) * 2017-05-25 2017-09-08 中国科学院上海高等研究院 一种表达外源基因的启动子及其应用
WO2019040412A1 (en) 2017-08-23 2019-02-28 Danisco Us Inc METHODS AND COMPOSITIONS FOR EFFICIENT GENETIC MODIFICATION OF BACILLUS LICHENIFORMIS STRAINS
KR20200047668A (ko) 2017-09-13 2020-05-07 다니스코 유에스 인크. 바실러스에서 증가된 단백질 생산을 위한 변형된 5'-비번역 영역(utr) 서열
CN108118058B (zh) * 2017-12-29 2021-06-29 苏州金唯智生物科技有限公司 一种改进的启动子及其应用
CN108118059B (zh) 2017-12-30 2021-03-19 苏州金唯智生物科技有限公司 一种改进的启动子及其组成的载体和应用
KR102715197B1 (ko) 2018-01-03 2024-10-08 다니스코 유에스 인크. 증가된 단백질 생산을 위한 돌연변이체 및 유전자 변형된 바실러스 세포 및 이의 방법
US20210277374A1 (en) 2018-07-06 2021-09-09 Dupont Nutrition Biosciences Aps Xylanase-containing feed additives for cereal-based animal feed
WO2020112609A1 (en) * 2018-11-28 2020-06-04 Danisco Us Inc Novel promoter sequences and methods thereof for enhanced protein production in bacillus cells
WO2020169564A1 (en) 2019-02-20 2020-08-27 Basf Se Industrial fermentation process for bacillus using defined medium and trace element feed
CN114096676A (zh) 2019-02-20 2022-02-25 巴斯夫欧洲公司 用确定成分培养基和镁补料的芽孢杆菌工业发酵工艺
KR102931179B1 (ko) 2019-04-05 2026-02-25 다니스코 유에스 인크. 선형 재조합 dna 작제물 및 이의 조성물을 이용하여 공여 dna 서열을 바실러스 게놈 내에 통합시키기 위한 방법
EP3947656A1 (de) 2019-04-05 2022-02-09 Danisco US Inc. Verfahren zur integration von polynukleotiden in das genom von bacillus unter verwendung von zwei zirkulären rekombinanten dna-konstrukten und zusammensetzungen davon
EP4031560A1 (de) 2019-08-14 2022-07-27 Danisco US Inc Zusammensetzungen und verfahren zur erhöhung der proteinproduktion in bacillus licheniformis
WO2021086606A1 (en) 2019-10-28 2021-05-06 Danisco Us Inc Microbial host cells for the production of heterologous cyanuric acid hydrolases and biuret hydrolases
WO2021096857A1 (en) 2019-11-11 2021-05-20 Danisco Us Inc Compositions and methods for enhanced protein production in bacillus cells
US12612649B2 (en) 2019-11-19 2026-04-28 Danisco Us Inc. Selection marker free methods for modifying the genome of bacillus and compositions thereof
CN114945665A (zh) 2020-01-15 2022-08-26 丹尼斯科美国公司 用于增强地衣芽孢杆菌中蛋白产生的组合物和方法
WO2021224152A1 (en) 2020-05-05 2021-11-11 Basf Se Improving expression in fermentation processes
CN113755492B (zh) * 2020-07-20 2023-05-30 中国科学院天津工业生物技术研究所 丙酮酸羧化酶基因启动子的突变体及其应用
WO2022178432A1 (en) 2021-02-22 2022-08-25 Danisco Us Inc. Methods and compositions for producing proteins of interest in pigment deficient bacillus cells
US20240263185A1 (en) 2021-05-24 2024-08-08 Danisco Us Inc. Compositions and methods for enhanced protein production in bacillus cells
US20240317820A1 (en) 2021-06-24 2024-09-26 Basf Se Improved bacillus production host
CN117693587A (zh) 2021-06-24 2024-03-12 巴斯夫欧洲公司 具有改变的RemA/RemB蛋白的改良芽孢杆菌宿主细胞
KR20240099301A (ko) 2021-11-16 2024-06-28 다니스코 유에스 인크. 바실러스 세포에서 단백질 생산을 향상시키기 위한 조성물 및 방법
CN119278264A (zh) * 2022-05-31 2025-01-07 松下知识产权经营株式会社 改造启动子、表达载体、微生物、物质的生产方法、改造蓝细菌和改造启动子的制作方法
WO2025034713A2 (en) 2023-08-09 2025-02-13 Danisco Us Inc. Compositions and methods for enhanced protein production in gram‑positive bacterial cells
KR20250148458A (ko) * 2024-04-01 2025-10-14 씨제이제일제당 (주) 신규한 바실러스 프로모터 변이체 및 이를 이용한 단백질 생산 방법

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4828994A (en) * 1984-09-21 1989-05-09 Genex Corporation Bacillus strains with reduced extracellular protease levels
DK87092D0 (da) * 1992-07-02 1992-07-02 Novo Nordisk As Nyt enzym
US6509185B1 (en) * 2000-01-07 2003-01-21 Genencor International, Inc. Mutant aprE promotor

Also Published As

Publication number Publication date
MXPA02006614A (es) 2004-09-10
CA2396250C (en) 2009-02-24
WO2001051643A1 (en) 2001-07-19
US6509185B1 (en) 2003-01-21
JP2003519492A (ja) 2003-06-24
CN101054588A (zh) 2007-10-17
AU2739001A (en) 2001-07-24
CN1313615C (zh) 2007-05-02
CA2396250A1 (en) 2001-07-19
EP1244794B1 (de) 2007-10-03
EP1244794A1 (de) 2002-10-02
JP4870306B2 (ja) 2012-02-08
DK1244794T3 (da) 2008-01-28
DE60036641T2 (de) 2008-07-03
KR20020073497A (ko) 2002-09-26
CN1425071A (zh) 2003-06-18
US20030148461A1 (en) 2003-08-07
DE60036641D1 (de) 2007-11-15
US6911322B2 (en) 2005-06-28

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