ATE374822T1 - Mutanter apre promoter - Google Patents
Mutanter apre promoterInfo
- Publication number
- ATE374822T1 ATE374822T1 AT00990354T AT00990354T ATE374822T1 AT E374822 T1 ATE374822 T1 AT E374822T1 AT 00990354 T AT00990354 T AT 00990354T AT 00990354 T AT00990354 T AT 00990354T AT E374822 T1 ATE374822 T1 AT E374822T1
- Authority
- AT
- Austria
- Prior art keywords
- mutant
- apre promoter
- promoter
- mutant apre
- apre
- Prior art date
Links
- 101150009206 aprE gene Proteins 0.000 abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 241000193830 Bacillus <bacterium> Species 0.000 abstract 1
- 244000063299 Bacillus subtilis Species 0.000 abstract 1
- 235000014469 Bacillus subtilis Nutrition 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/479,494 US6509185B1 (en) | 2000-01-07 | 2000-01-07 | Mutant aprE promotor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE374822T1 true ATE374822T1 (de) | 2007-10-15 |
Family
ID=23904251
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT00990354T ATE374822T1 (de) | 2000-01-07 | 2000-12-21 | Mutanter apre promoter |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US6509185B1 (de) |
| EP (1) | EP1244794B1 (de) |
| JP (1) | JP4870306B2 (de) |
| KR (1) | KR20020073497A (de) |
| CN (2) | CN1313615C (de) |
| AT (1) | ATE374822T1 (de) |
| AU (1) | AU2739001A (de) |
| CA (1) | CA2396250C (de) |
| DE (1) | DE60036641T2 (de) |
| DK (1) | DK1244794T3 (de) |
| MX (1) | MXPA02006614A (de) |
| WO (1) | WO2001051643A1 (de) |
Families Citing this family (48)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6509185B1 (en) * | 2000-01-07 | 2003-01-21 | Genencor International, Inc. | Mutant aprE promotor |
| ATE495245T1 (de) * | 2002-02-15 | 2011-01-15 | Danisco Us Inc | Verbesserte proteinexpression in bacillus subtilis |
| EP1495128B1 (de) * | 2002-03-29 | 2014-05-07 | Genencor International, Inc. | Verstärkte proteinexpression in bacillus |
| US8343735B2 (en) * | 2007-05-10 | 2013-01-01 | Danisco Us Inc. | Modified secretion system to increase expression of polypeptides in bacteria |
| US20090048136A1 (en) * | 2007-08-15 | 2009-02-19 | Mcdonald Hugh C | Kappa-carrageenase and kappa-carrageenase-containing compositions |
| DK2195435T3 (da) | 2007-09-12 | 2012-12-10 | Danisco Us Inc | Trichoderma-promoter |
| JP5629584B2 (ja) | 2007-12-21 | 2014-11-19 | ダニスコ・ユーエス・インク | バチルス内での促進されたタンパク質生成 |
| AR076941A1 (es) | 2009-06-11 | 2011-07-20 | Danisco Us Inc | Cepa de bacillus para una mayor produccion de proteina |
| US20140329309A1 (en) | 2011-12-09 | 2014-11-06 | Danisco Us Inc. | Ribosomal Promoters for Production in Microorganisms |
| US10683528B2 (en) * | 2014-12-16 | 2020-06-16 | Danisco Us Inc | Enhanced protein expression |
| KR20180069057A (ko) | 2015-10-30 | 2018-06-22 | 다니스코 유에스 인크. | 향상된 단백질 발현 및 이의 방법 |
| US20180371445A1 (en) | 2015-12-23 | 2018-12-27 | Danisco Us Inc. | Enhanced protein production and methods thereof |
| KR20180117166A (ko) | 2016-03-04 | 2018-10-26 | 다니스코 유에스 인크. | 미생물에서의 단백질 생산을 위한 유전자 조작 리보솜 프로모터 |
| EP4644560A3 (de) | 2016-12-21 | 2026-04-08 | International N&H Denmark ApS | Verfahren zur verwendung von thermostabilen serinproteasen |
| WO2018136459A1 (en) | 2017-01-23 | 2018-07-26 | Danisco Us Inc. | Methods and compositions for obtaining natural competence in bacillus host cells |
| EP3585910B1 (de) | 2017-02-24 | 2024-03-20 | Danisco US Inc. | Zusammensetzungen und verfahren zur erhöhung der proteinproduktion in bacillus licheniformis |
| WO2018169780A1 (en) | 2017-03-15 | 2018-09-20 | Dupont Nutrition Biosciences Aps | Methods of using an archaeal serine protease |
| WO2018169784A1 (en) | 2017-03-15 | 2018-09-20 | Dupont Nutrition Biosciences Aps | Trypsin-like serine proteases and uses thereof cross-reference to related application |
| CN110621778A (zh) | 2017-03-15 | 2019-12-27 | 丹尼斯科美国公司 | 胰蛋白酶样丝氨酸蛋白酶及其用途 |
| FI3607097T3 (fi) | 2017-04-07 | 2023-09-11 | Dupont Nutrition Biosci Aps | BEETA-GALAKTOSIDAASEJA JA LAKTAASEJA ILMAN p-NITROBENTSYYLIESTERASISIVUAKTIIVISUUTTA TUOTTAVIA BACILLUS-ISÄNTÄSOLUJA |
| CN107142259A (zh) * | 2017-05-25 | 2017-09-08 | 中国科学院上海高等研究院 | 一种表达外源基因的启动子及其应用 |
| US11879127B2 (en) | 2017-08-23 | 2024-01-23 | Danisco Us Inc. | Methods and compositions for efficient genetic modifications of Bacillus licheniformis strains |
| US20210032639A1 (en) | 2017-09-13 | 2021-02-04 | Danisco Us Inc. | Modified 5'-untranslated region (utr) sequences for increased protein production in bacillus |
| CN108118058B (zh) | 2017-12-29 | 2021-06-29 | 苏州金唯智生物科技有限公司 | 一种改进的启动子及其应用 |
| CN108118059B (zh) | 2017-12-30 | 2021-03-19 | 苏州金唯智生物科技有限公司 | 一种改进的启动子及其组成的载体和应用 |
| CN111670244B (zh) | 2018-01-03 | 2023-08-01 | 丹尼斯科美国公司 | 突变的和遗传修饰的芽孢杆菌属细胞及其用于增加蛋白质产生的方法 |
| BR112021000116A2 (pt) | 2018-07-06 | 2021-04-06 | Dupont Nutrition Biosciences Aps | Aditivos de alimento contendo xilanase para alimento de animal à base de cereal |
| US11781147B2 (en) * | 2018-11-28 | 2023-10-10 | Danisco Us Inc. | Promoter sequences and methods thereof for enhanced protein production in Bacillus cells |
| WO2020169563A1 (en) | 2019-02-20 | 2020-08-27 | Basf Se | Industrial fermentation process for bacillus using defined medium and magnesium feed |
| US20220186234A1 (en) | 2019-02-20 | 2022-06-16 | Basf Se | Industrial Fermentation Process for Bacillus Using Defined Medium and Trace Element Feed |
| CA3136113A1 (en) | 2019-04-05 | 2020-10-08 | Danisco Us Inc. | Methods for polynucleotide integration into the genome of bacillus using dual circular recombinant dna constructs and compositions thereof |
| JP2022526982A (ja) | 2019-04-05 | 2022-05-27 | ダニスコ・ユーエス・インク | 線状組換えＤＮＡコンストラクトを使用してバチルス（Ｂａｃｉｌｌｕｓ）のゲノムにドナーＤＮＡ配列を組み込むための方法及びその組成物 |
| KR20220041928A (ko) | 2019-08-14 | 2022-04-01 | 다니스코 유에스 인크. | 바실러스 리체니포르미스에서 단백질 생산을 증가시키기 위한 조성물 및 방법 |
| WO2021086606A1 (en) | 2019-10-28 | 2021-05-06 | Danisco Us Inc | Microbial host cells for the production of heterologous cyanuric acid hydrolases and biuret hydrolases |
| JP7656603B2 (ja) | 2019-11-11 | 2025-04-03 | ダニスコ・ユーエス・インク | バチルス属（ｂａｃｉｌｌｕｓ）細胞における強化されたタンパク質産生のための組成物及び方法 |
| WO2021101950A1 (en) | 2019-11-19 | 2021-05-27 | Danisco Us Inc | Selection marker free methods for modifying the genome of bacillus and compositions thereof |
| JP7787077B2 (ja) | 2020-01-15 | 2025-12-16 | ダニスコ・ユーエス・インク | バチルス・リケニフォルミス（ｂａｃｉｌｌｕｓ ｌｉｃｈｅｎｉｆｏｒｍｉｓ）における強化したタンパク質産生のための組成物及び方法 |
| WO2021224152A1 (en) | 2020-05-05 | 2021-11-11 | Basf Se | Improving expression in fermentation processes |
| CN113755492B (zh) * | 2020-07-20 | 2023-05-30 | 中国科学院天津工业生物技术研究所 | 丙酮酸羧化酶基因启动子的突变体及其应用 |
| CN116897160A (zh) | 2021-02-22 | 2023-10-17 | 丹尼斯科美国公司 | 在色素缺陷型芽孢杆菌属细胞中产生目的蛋白的方法和组合物 |
| CN117769597A (zh) | 2021-05-24 | 2024-03-26 | 丹尼斯科美国公司 | 用于增强芽孢杆菌属细胞中蛋白质产生的组合物和方法 |
| CN117813317A (zh) | 2021-06-24 | 2024-04-02 | 巴斯夫欧洲公司 | 改良的芽孢杆菌生产宿主 |
| US20240294894A1 (en) | 2021-06-24 | 2024-09-05 | Basf Se | Improved bacillus host cell with altered rema/remb protein |
| KR20240099301A (ko) | 2021-11-16 | 2024-06-28 | 다니스코 유에스 인크. | 바실러스 세포에서 단백질 생산을 향상시키기 위한 조성물 및 방법 |
| JPWO2023233996A1 (de) * | 2022-05-31 | 2023-12-07 | ||
| CN121889413A (zh) | 2023-08-09 | 2026-04-17 | 丹尼斯科美国公司 | 用于增强革兰氏阳性细菌细胞中的蛋白质产生的组合物和方法 |
| KR20250148458A (ko) * | 2024-04-01 | 2025-10-14 | 씨제이제일제당 (주) | 신규한 바실러스 프로모터 변이체 및 이를 이용한 단백질 생산 방법 |
| WO2026090396A1 (en) | 2024-10-24 | 2026-04-30 | Danisco Us Inc. | Compositions and methods for enhanced protein production in gram‑positive bacterial cells |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4828994A (en) * | 1984-09-21 | 1989-05-09 | Genex Corporation | Bacillus strains with reduced extracellular protease levels |
| DK87092D0 (da) * | 1992-07-02 | 1992-07-02 | Novo Nordisk As | Nyt enzym |
| US6509185B1 (en) * | 2000-01-07 | 2003-01-21 | Genencor International, Inc. | Mutant aprE promotor |
-
2000
- 2000-01-07 US US09/479,494 patent/US6509185B1/en not_active Expired - Lifetime
- 2000-12-21 DE DE60036641T patent/DE60036641T2/de not_active Expired - Lifetime
- 2000-12-21 EP EP00990354A patent/EP1244794B1/de not_active Expired - Lifetime
- 2000-12-21 CN CNB008186197A patent/CN1313615C/zh not_active Expired - Fee Related
- 2000-12-21 CN CNA2007100862906A patent/CN101054588A/zh active Pending
- 2000-12-21 AU AU27390/01A patent/AU2739001A/en not_active Abandoned
- 2000-12-21 MX MXPA02006614A patent/MXPA02006614A/es unknown
- 2000-12-21 AT AT00990354T patent/ATE374822T1/de not_active IP Right Cessation
- 2000-12-21 DK DK00990354T patent/DK1244794T3/da active
- 2000-12-21 KR KR1020027008769A patent/KR20020073497A/ko not_active Withdrawn
- 2000-12-21 JP JP2001551217A patent/JP4870306B2/ja not_active Expired - Fee Related
- 2000-12-21 WO PCT/US2000/035312 patent/WO2001051643A1/en not_active Ceased
- 2000-12-21 CA CA002396250A patent/CA2396250C/en not_active Expired - Fee Related
-
2002
- 2002-12-03 US US10/308,368 patent/US6911322B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| MXPA02006614A (es) | 2004-09-10 |
| EP1244794B1 (de) | 2007-10-03 |
| EP1244794A1 (de) | 2002-10-02 |
| JP2003519492A (ja) | 2003-06-24 |
| CA2396250A1 (en) | 2001-07-19 |
| DK1244794T3 (da) | 2008-01-28 |
| KR20020073497A (ko) | 2002-09-26 |
| CA2396250C (en) | 2009-02-24 |
| US20030148461A1 (en) | 2003-08-07 |
| CN101054588A (zh) | 2007-10-17 |
| AU2739001A (en) | 2001-07-24 |
| DE60036641D1 (de) | 2007-11-15 |
| JP4870306B2 (ja) | 2012-02-08 |
| CN1425071A (zh) | 2003-06-18 |
| CN1313615C (zh) | 2007-05-02 |
| WO2001051643A1 (en) | 2001-07-19 |
| DE60036641T2 (de) | 2008-07-03 |
| US6509185B1 (en) | 2003-01-21 |
| US6911322B2 (en) | 2005-06-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |