ATE374822T1 - Mutanter apre promoter - Google Patents

Mutanter apre promoter

Info

Publication number
ATE374822T1
ATE374822T1 AT00990354T AT00990354T ATE374822T1 AT E374822 T1 ATE374822 T1 AT E374822T1 AT 00990354 T AT00990354 T AT 00990354T AT 00990354 T AT00990354 T AT 00990354T AT E374822 T1 ATE374822 T1 AT E374822T1
Authority
AT
Austria
Prior art keywords
mutant
apre promoter
promoter
mutant apre
apre
Prior art date
Application number
AT00990354T
Other languages
English (en)
Inventor
Eugenio Ferrari
Fernando Valle
Original Assignee
Genencor Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Int filed Critical Genencor Int
Application granted granted Critical
Publication of ATE374822T1 publication Critical patent/ATE374822T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
AT00990354T 2000-01-07 2000-12-21 Mutanter apre promoter ATE374822T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/479,494 US6509185B1 (en) 2000-01-07 2000-01-07 Mutant aprE promotor

Publications (1)

Publication Number Publication Date
ATE374822T1 true ATE374822T1 (de) 2007-10-15

Family

ID=23904251

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00990354T ATE374822T1 (de) 2000-01-07 2000-12-21 Mutanter apre promoter

Country Status (12)

Country Link
US (2) US6509185B1 (de)
EP (1) EP1244794B1 (de)
JP (1) JP4870306B2 (de)
KR (1) KR20020073497A (de)
CN (2) CN101054588A (de)
AT (1) ATE374822T1 (de)
AU (1) AU2739001A (de)
CA (1) CA2396250C (de)
DE (1) DE60036641T2 (de)
DK (1) DK1244794T3 (de)
MX (1) MXPA02006614A (de)
WO (1) WO2001051643A1 (de)

Families Citing this family (47)

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US6509185B1 (en) * 2000-01-07 2003-01-21 Genencor International, Inc. Mutant aprE promotor
WO2003070963A2 (en) * 2002-02-15 2003-08-28 Genencor International, Inc. Improved protein expression in bacillus subtilis
EP2341070A3 (de) 2002-03-29 2011-10-19 Genencor International, Inc. Verstärkte Proteinexpression in Bacillus
CN101679489B (zh) 2007-05-10 2014-05-28 丹尼斯科美国公司 增加细菌内多肽表达的修饰的分泌系统
US20090048136A1 (en) * 2007-08-15 2009-02-19 Mcdonald Hugh C Kappa-carrageenase and kappa-carrageenase-containing compositions
EP2195435B1 (de) 2007-09-12 2012-10-10 Danisco US Inc. Trichoderma-promotor
KR20100098652A (ko) 2007-12-21 2010-09-08 다니스코 유에스 인크. 바실루스 내에서의 향상된 단백질 생산
AR076941A1 (es) 2009-06-11 2011-07-20 Danisco Us Inc Cepa de bacillus para una mayor produccion de proteina
EP2788491B1 (de) 2011-12-09 2019-01-23 Danisco US Inc. Ribosomale promotoren aus b. subtilis zur proteinproduktion in mikroorganismen
KR102588719B1 (ko) * 2014-12-16 2023-10-12 다니스코 유에스 인크. 향상된 단백질 발현
JP7025325B2 (ja) 2015-10-30 2022-02-24 ダニスコ・ユーエス・インク タンパク質発現の増強およびその方法
JP6993339B2 (ja) 2015-12-23 2022-02-21 ダニスコ・ユーエス・インク タンパク質産生の強化およびその方法
EP4095152A3 (de) 2016-03-04 2022-12-28 Danisco US Inc. Manipulierte ribosomale promotoren zur proteinerzeugung in mikroorganismen
MX2019007335A (es) 2016-12-21 2019-09-09 Dupont Nutrition Biosci Aps Métodos para usar serina proteasas termoestables.
US20190367932A1 (en) 2017-01-23 2019-12-05 Danisco Us Inc. Methods and compositions for obtaining natural comptence in bacillus host cells
FI3585910T3 (fi) 2017-02-24 2024-06-19 Danisco Us Inc Koostumukset ja menetelmät proteiinin tuotannon lisäämiseksi bacillus licheniformiksessa
WO2018169750A1 (en) 2017-03-15 2018-09-20 Danisco Us Inc Trypsin-like serine proteases and uses thereof
US20200015499A1 (en) 2017-03-15 2020-01-16 Dupont Nutrition Biosciences Aps Trypsin-like serine proteases and uses thereof
MX386582B (es) 2017-03-15 2025-03-19 Int N&H Denmark Aps Métodos para usar una serina proteasa de arqueas.
EP3607097B1 (de) 2017-04-07 2023-06-21 DuPont Nutrition Biosciences ApS Bacillus-wirtszellen, welche beta-galactosidasen und lactasen in abwesenheit von p-nitrobenzylesterase nebenaktivität produzieren
CN107142259A (zh) * 2017-05-25 2017-09-08 中国科学院上海高等研究院 一种表达外源基因的启动子及其应用
EP3655537A1 (de) 2017-08-23 2020-05-27 Danisco US Inc. Verfahren und zusammensetzungen zur effizienten genetischen modifikation von bacillus-licheniformis-stämmen
WO2019055261A1 (en) 2017-09-13 2019-03-21 Danisco Us Inc MODIFIED 5 'NON-TRANSLATED REGION (UTR) SEQUENCES FOR INCREASED PROTEIN PRODUCTION IN BACILLUS
CN108118058B (zh) 2017-12-29 2021-06-29 苏州金唯智生物科技有限公司 一种改进的启动子及其应用
CN108118059B (zh) * 2017-12-30 2021-03-19 苏州金唯智生物科技有限公司 一种改进的启动子及其组成的载体和应用
WO2019135868A1 (en) 2018-01-03 2019-07-11 Danisco Us Inc Mutant and genetically modified bacillus cells and methods thereof for increased protein production
CN112654703A (zh) 2018-07-06 2021-04-13 杜邦营养生物科学有限公司 用于基于谷类的动物饲料的含木聚糖酶饲料添加剂
US11781147B2 (en) * 2018-11-28 2023-10-10 Danisco Us Inc. Promoter sequences and methods thereof for enhanced protein production in Bacillus cells
WO2020169564A1 (en) 2019-02-20 2020-08-27 Basf Se Industrial fermentation process for bacillus using defined medium and trace element feed
MX2021010110A (es) 2019-02-20 2021-09-21 Basf Se Proceso de fermentacion industrial para bacillus mediante el uso de medio definido y alimentacion de magnesio.
CA3136114A1 (en) 2019-04-05 2020-10-08 Danisco Us Inc. Methods for integrating a donor dna sequence into the genome of bacillus using linear recombinant dna constructs and compositions thereof
EP3947656A1 (de) 2019-04-05 2022-02-09 Danisco US Inc. Verfahren zur integration von polynukleotiden in das genom von bacillus unter verwendung von zwei zirkulären rekombinanten dna-konstrukten und zusammensetzungen davon
JP7676362B2 (ja) 2019-08-14 2025-05-14 ダニスコ・ユーエス・インク バチルス・リケニフォルミス（ｂａｃｉｌｌｕｓ ｌｉｃｈｅｎｉｆｏｒｍｉｓ）における増加したタンパク質産生のための組成物及び方法
CA3155372A1 (en) 2019-10-28 2021-05-06 Sharief Barends Microbial host cells for the production of heterologous cyanuric acid hydrolases and biuret hydrolases
EP4058553A1 (de) 2019-11-11 2022-09-21 Danisco US Inc. Zusammensetzungen und verfahren zur verbesserten proteinproduktion in bacilluszellen
WO2021101950A1 (en) 2019-11-19 2021-05-27 Danisco Us Inc Selection marker free methods for modifying the genome of bacillus and compositions thereof
WO2021146411A1 (en) 2020-01-15 2021-07-22 Danisco Us Inc Compositions and methods for enhanced protein production in bacillus licheniformis
WO2021224152A1 (en) 2020-05-05 2021-11-11 Basf Se Improving expression in fermentation processes
CN113755492B (zh) * 2020-07-20 2023-05-30 中国科学院天津工业生物技术研究所 丙酮酸羧化酶基因启动子的突变体及其应用
CN116897160A (zh) 2021-02-22 2023-10-17 丹尼斯科美国公司 在色素缺陷型芽孢杆菌属细胞中产生目的蛋白的方法和组合物
EP4347812A1 (de) 2021-05-24 2024-04-10 Danisco US Inc. Zusammensetzungen und verfahren zur verbesserten proteinproduktion in bacillus-zellen
CN117813317A (zh) 2021-06-24 2024-04-02 巴斯夫欧洲公司 改良的芽孢杆菌生产宿主
WO2022269084A1 (en) 2021-06-24 2022-12-29 Basf Se Improved bacillus host cell with altered rema/remb protein
JP2024543466A (ja) 2021-11-16 2024-11-21 ダニスコ・ユーエス・インク バチルス属（ｂａｃｉｌｌｕｓ）細胞における強化されたタンパク質産生のための組成物及び方法
JPWO2023233996A1 (de) * 2022-05-31 2023-12-07
WO2025034713A2 (en) 2023-08-09 2025-02-13 Danisco Us Inc. Compositions and methods for enhanced protein production in gram‑positive bacterial cells
KR20250148458A (ko) * 2024-04-01 2025-10-14 씨제이제일제당 (주) 신규한 바실러스 프로모터 변이체 및 이를 이용한 단백질 생산 방법

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4828994A (en) * 1984-09-21 1989-05-09 Genex Corporation Bacillus strains with reduced extracellular protease levels
DK87092D0 (da) * 1992-07-02 1992-07-02 Novo Nordisk As Nyt enzym
US6509185B1 (en) * 2000-01-07 2003-01-21 Genencor International, Inc. Mutant aprE promotor

Also Published As

Publication number Publication date
CA2396250C (en) 2009-02-24
WO2001051643A1 (en) 2001-07-19
EP1244794A1 (de) 2002-10-02
CN1313615C (zh) 2007-05-02
US6911322B2 (en) 2005-06-28
US20030148461A1 (en) 2003-08-07
KR20020073497A (ko) 2002-09-26
JP2003519492A (ja) 2003-06-24
CN1425071A (zh) 2003-06-18
US6509185B1 (en) 2003-01-21
MXPA02006614A (es) 2004-09-10
JP4870306B2 (ja) 2012-02-08
DK1244794T3 (da) 2008-01-28
AU2739001A (en) 2001-07-24
CN101054588A (zh) 2007-10-17
CA2396250A1 (en) 2001-07-19
DE60036641D1 (de) 2007-11-15
EP1244794B1 (de) 2007-10-03
DE60036641T2 (de) 2008-07-03

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