ATE375510T1 - Screening nach bindepartnern und deren design durch röntgenkristallographie - Google Patents

Screening nach bindepartnern und deren design durch röntgenkristallographie

Info

Publication number
ATE375510T1
ATE375510T1 AT99911174T AT99911174T ATE375510T1 AT E375510 T1 ATE375510 T1 AT E375510T1 AT 99911174 T AT99911174 T AT 99911174T AT 99911174 T AT99911174 T AT 99911174T AT E375510 T1 ATE375510 T1 AT E375510T1
Authority
AT
Austria
Prior art keywords
biomolecule
crystal
ligand
test samples
target
Prior art date
Application number
AT99911174T
Other languages
English (en)
Inventor
Vicki Nienaber
Jonathan Greer
Celerino Abad-Zapatero
Daniel Norbeck
Original Assignee
Abbott Lab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Lab filed Critical Abbott Lab
Application granted granted Critical
Publication of ATE375510T1 publication Critical patent/ATE375510T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • C—CHEMISTRY; METALLURGY
    • C30—CRYSTAL GROWTH
    • C30B—SINGLE-CRYSTAL GROWTH; UNIDIRECTIONAL SOLIDIFICATION OF EUTECTIC MATERIAL OR UNIDIRECTIONAL DEMIXING OF EUTECTOID MATERIAL; REFINING BY ZONE-MELTING OF MATERIAL; PRODUCTION OF A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; SINGLE CRYSTALS OR HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; AFTER-TREATMENT OF SINGLE CRYSTALS OR A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; APPARATUS THEREFOR
    • C30B7/00—Single-crystal growth from solutions using solvents which are liquid at normal temperature, e.g. aqueous solutions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N23/00—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00
    • G01N23/20—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by using diffraction of the radiation by the materials, e.g. for investigating crystal structure; by using scattering of the radiation by the materials, e.g. for investigating non-crystalline materials; by using reflection of the radiation by the materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2299/00—Coordinates from 3D structures of peptides, e.g. proteins or enzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/972—Plasminogen activators
    • G01N2333/9723—Urokinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Materials Engineering (AREA)
  • Metallurgy (AREA)
  • Organic Chemistry (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT99911174T 1998-03-06 1999-03-05 Screening nach bindepartnern und deren design durch röntgenkristallographie ATE375510T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US3618498A 1998-03-06 1998-03-06

Publications (1)

Publication Number Publication Date
ATE375510T1 true ATE375510T1 (de) 2007-10-15

Family

ID=21887121

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99911174T ATE375510T1 (de) 1998-03-06 1999-03-05 Screening nach bindepartnern und deren design durch röntgenkristallographie

Country Status (18)

Country Link
US (1) US6297021B1 (de)
EP (1) EP1068531B1 (de)
JP (1) JP2002506206A (de)
KR (1) KR20010041653A (de)
CN (2) CN100354627C (de)
AT (1) ATE375510T1 (de)
AU (3) AU2887099A (de)
BG (1) BG104802A (de)
BR (1) BR9908563A (de)
CA (1) CA2321968A1 (de)
DE (1) DE69937292T2 (de)
HU (1) HUP0101959A3 (de)
IL (1) IL137758A0 (de)
NO (1) NO20004433L (de)
PL (1) PL343264A1 (de)
SK (1) SK13242000A3 (de)
TR (4) TR200101127T2 (de)
WO (2) WO1999045389A2 (de)

Families Citing this family (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030049678A1 (en) * 1999-03-05 2003-03-13 Nienaber Vicki L. Ligand screening and design by X-ray crystallography
US20030022383A1 (en) * 1999-04-06 2003-01-30 Uab Research Foundation Method for screening crystallization conditions in solution crystal growth
US7247490B2 (en) 1999-04-06 2007-07-24 Uab Research Foundation Method for screening crystallization conditions in solution crystal growth
US7214540B2 (en) * 1999-04-06 2007-05-08 Uab Research Foundation Method for screening crystallization conditions in solution crystal growth
US7244396B2 (en) 1999-04-06 2007-07-17 Uab Research Foundation Method for preparation of microarrays for screening of crystal growth conditions
US7250305B2 (en) 2001-07-30 2007-07-31 Uab Research Foundation Use of dye to distinguish salt and protein crystals under microcrystallization conditions
US6630006B2 (en) * 1999-06-18 2003-10-07 The Regents Of The University Of California Method for screening microcrystallizations for crystal formation
US20040252299A9 (en) 2000-01-07 2004-12-16 Lemmo Anthony V. Apparatus and method for high-throughput preparation and spectroscopic classification and characterization of compositions
US7108970B2 (en) 2000-01-07 2006-09-19 Transform Pharmaceuticals, Inc. Rapid identification of conditions, compounds, or compositions that inhibit, prevent, induce, modify, or reverse transitions of physical state
GB0008563D0 (en) 2000-04-07 2000-05-24 Cambridge Discovery Chemistry Investigating different physical and/or chemical forms of materials
WO2002022154A2 (en) * 2000-09-13 2002-03-21 Praecis Pharmaceuticals Incorporated Pharmaceutical compositions for sustained drug delivery
US7806980B2 (en) 2000-12-23 2010-10-05 Elan Pharmaceuticals, Inc. Method for crystallizing human beta secretase in complex with an inhibitor
US7601528B1 (en) 2000-12-23 2009-10-13 Elan Pharmaceuticals, Inc. Crystallization and structure determination of glycosylated human beta secretase, an enzyme implicated in alzheimer's disease
US7217556B1 (en) 2000-12-23 2007-05-15 Pfizer Inc Crystallization and structure determination of glycosylated human beta secretase, an enzyme implicated in Alzheimer's disease
US7670429B2 (en) 2001-04-05 2010-03-02 The California Institute Of Technology High throughput screening of crystallization of materials
WO2002083938A2 (en) * 2001-04-11 2002-10-24 Emerald Biostructures, Inc. Screening methods for identifying ligands
US7384773B1 (en) * 2001-05-10 2008-06-10 Pfizer Inc Crystal of HIV protease-cleaved human beta secretase and method for crystallization thereof
US7524668B1 (en) * 2001-05-10 2009-04-28 Elan Pharmaceuticals, Inc. Crystal of human beta secretase having monoclinic space group symmetry C2 and methods for crystallization thereof
US7130747B1 (en) * 2001-05-31 2006-10-31 Los Alamos National Security, Llc High throughput screening of ligand binding to macromolecules using high resolution powder diffraction
IL151012A0 (en) * 2001-08-03 2003-02-12 Ribosomes Structure And Protei Ribosomes structure and protein synthesis inhibitors
AU2002333508A1 (en) * 2001-08-22 2003-03-10 Novartis Ag Method to identify modulators for human 3alpha-hydroxysteroid dehydrogenase
US20030211538A1 (en) * 2001-12-03 2003-11-13 Ming Luo CAP-Gly domain structure and uses thereof
US20040171062A1 (en) * 2002-02-28 2004-09-02 Plexxikon, Inc. Methods for the design of molecular scaffolds and ligands
US7469036B2 (en) * 2002-04-19 2008-12-23 Los Alamos National Security, Llc Analysis of macromolecules, ligands and macromolecule-ligand complexes
US7442537B1 (en) 2002-05-10 2008-10-28 Elan Pharmaceuticals, Inc. Crystals of unliganded beta secretase and/or beta secretase-like proteins and the use thereof
US6989392B2 (en) * 2002-06-18 2006-01-24 Abbott Laboratories 2-Aminoquinolines as melanin concentrating hormone receptor antagonists
US8019550B2 (en) * 2002-07-24 2011-09-13 Keddem Bioscience Ltd. Drug discovery method
AU2003272548A1 (en) * 2002-09-16 2004-04-30 Plexxikon, Inc. Crystal structure of pim-1 kinase
WO2006078228A1 (en) * 2002-09-16 2006-07-27 Plexxikon, Inc. Methods for the design of molecular scaffolds and ligands
JP2006516095A (ja) * 2002-11-14 2006-06-22 ザ スクリップス リサーチ インスティテュート 脂肪酸アミドヒドロラーゼ（ｆａａｈ）の結晶形
ATE354096T1 (de) * 2002-12-23 2007-03-15 Astex Therapeutics Ltd Synthese und screening von liganden mit hilfe von röntgenkristallographie
JP2007524374A (ja) * 2003-02-28 2007-08-30 プレキシコン，インコーポレーテッド Ｐｙｋ２結晶構造および使用
WO2004092727A2 (en) * 2003-04-11 2004-10-28 Structural Genomix, Inc. Compound libraries and methods for drug discovery
US20050079548A1 (en) * 2003-07-07 2005-04-14 Plexxikon, Inc. Ligand development using PDE4B crystal structures
WO2005017533A1 (de) * 2003-08-06 2005-02-24 Proteros Biostructures Gmbh Verfahren zur identifizierung von schwach bindenden molekülfragmenten mit ligandeneigenschaften, wobei die molekülfragmente als mikrotropfen einer entsprechenden lösung auf den kristall aufgebracht werden
DE10336110B4 (de) * 2003-08-06 2008-01-03 Proteros Biostructures Gmbh Vorrichtung und Verfahren zum Behandeln eines Proteinkristalls
WO2005028624A2 (en) * 2003-09-15 2005-03-31 Plexxikon, Inc. Molecular scaffolds for kinase ligand development
DE10343522B4 (de) * 2003-09-19 2008-09-18 Proteros Biostructures Gmbh Verfahren und Speichermedium zur Steuerung der Behandlung eines Kristalls mit einer Flüssigkeit
EP1899370A4 (de) * 2005-05-12 2009-11-11 Cytopia Res Pty Ltd Kristallstruktur und anwendungen davon
US20090317850A1 (en) * 2008-06-20 2009-12-24 Tomoko Sunami Crystal Structure of Human 70KD Ribosomal Protein S6 Kinase 1 Kinase Domain
CN101339167B (zh) * 2008-08-27 2011-12-14 中国药科大学 基于靶蛋白亲和选择的活性成分高通量筛选方法
DE102008049675A1 (de) 2008-09-30 2010-04-01 Markus Dr. Heinrich Verfahren zur Herstellung von 3-Aminobiphenylen
WO2010100847A1 (ja) * 2009-03-03 2010-09-10 独立行政法人国立高等専門学校機構 生体高分子の結晶化装置、生体高分子の結晶化溶液セル、生体高分子の配向制御方法、生体高分子の結晶化方法、及び生体高分子の結晶
CN102101886A (zh) 2009-12-18 2011-06-22 四川辉阳生命工程股份有限公司 构象改变的重组干扰素晶体、其三维结构及应用
JP5598238B2 (ja) * 2010-10-08 2014-10-01 株式会社リコー 画像形成装置
CN106033064B (zh) * 2015-03-10 2019-05-07 上海上药信谊药厂有限公司 胆维丁测定方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5112755A (en) 1982-04-15 1992-05-12 Genentech, Inc. Preparation of functional human urokinase proteins

Also Published As

Publication number Publication date
TR200101129T2 (tr) 2002-06-21
CN100354627C (zh) 2007-12-12
AU2987899A (en) 1999-09-20
HUP0101959A3 (en) 2003-10-28
AU2003255167A1 (en) 2003-11-13
EP1068531A2 (de) 2001-01-17
TR200002572T2 (tr) 2000-11-21
IL137758A0 (en) 2001-10-31
DE69937292D1 (de) 2007-11-22
AU2887099A (en) 1999-09-20
WO1999045389A2 (en) 1999-09-10
NO20004433L (no) 2000-11-06
WO1999045389A3 (en) 2000-02-10
US6297021B1 (en) 2001-10-02
WO1999045379A3 (en) 1999-11-25
JP2002506206A (ja) 2002-02-26
TR200101200T2 (tr) 2002-02-21
CN101221138A (zh) 2008-07-16
WO1999045379A2 (en) 1999-09-10
NO20004433D0 (no) 2000-09-05
HUP0101959A2 (hu) 2001-09-28
TR200101127T2 (tr) 2002-06-21
EP1068531B1 (de) 2007-10-10
BG104802A (bg) 2001-04-30
CA2321968A1 (en) 1999-09-10
KR20010041653A (ko) 2001-05-25
CN1299467A (zh) 2001-06-13
DE69937292T2 (de) 2008-07-03
PL343264A1 (en) 2001-07-30
AU767991B2 (en) 2003-11-27
BR9908563A (pt) 2000-11-21
SK13242000A3 (sk) 2001-03-12

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