ATE377647T1 - Durch kälte induzierbarer expressionsvektor - Google Patents

Durch kälte induzierbarer expressionsvektor

Info

Publication number
ATE377647T1
ATE377647T1 AT98953080T AT98953080T ATE377647T1 AT E377647 T1 ATE377647 T1 AT E377647T1 AT 98953080 T AT98953080 T AT 98953080T AT 98953080 T AT98953080 T AT 98953080T AT E377647 T1 ATE377647 T1 AT E377647T1
Authority
AT
Austria
Prior art keywords
region
cold
expression vector
promoter
codes
Prior art date
Application number
AT98953080T
Other languages
English (en)
Inventor
Masanori Takayama
Yoshiko Nomura
Ikunoshin Kato
Original Assignee
Takara Bio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Takara Bio Inc filed Critical Takara Bio Inc
Application granted granted Critical
Publication of ATE377647T1 publication Critical patent/ATE377647T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/72—Expression systems using regulatory sequences derived from the lac-operon
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
    • C12N9/2471—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Pulse, Heart Rate, Blood Pressure Or Blood Flow (AREA)
  • Non-Portable Lighting Devices Or Systems Thereof (AREA)
  • Eye Examination Apparatus (AREA)
AT98953080T 1997-11-20 1998-11-17 Durch kälte induzierbarer expressionsvektor ATE377647T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP33479297 1997-11-20

Publications (1)

Publication Number Publication Date
ATE377647T1 true ATE377647T1 (de) 2007-11-15

Family

ID=18281290

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98953080T ATE377647T1 (de) 1997-11-20 1998-11-17 Durch kälte induzierbarer expressionsvektor

Country Status (9)

Country Link
US (2) US6479260B1 (de)
EP (1) EP1033408B1 (de)
JP (1) JP4057237B2 (de)
KR (2) KR100596071B1 (de)
AT (1) ATE377647T1 (de)
AU (1) AU1054699A (de)
CA (1) CA2309600C (de)
DE (1) DE69838680T2 (de)
WO (1) WO1999027117A1 (de)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5981280A (en) 1996-03-22 1999-11-09 The University Of Medicine And Denistry Of New Jersey Method and constructs for inhibiting protein expression in bacteria
KR100596071B1 (ko) 1997-11-20 2006-07-03 다카라 바이오 가부시키가이샤 저온 유도성 발현벡터
EP1053310A1 (de) * 1998-08-20 2000-11-22 University Of Medicine And Dentistry Of New Jersey Kälteschockprotein regulatorische elemente, konstrukte davon, und methoden zu deren anwendung
US6448007B1 (en) * 1999-07-02 2002-09-10 Message Pharmaceuticals Functional genomic screen for post-transcriptional 5′ and 3′ regulatory elements
CN100385004C (zh) * 2002-03-01 2008-04-30 宝生物工程株式会社 冷休克诱导的异源多肽的表达和生产
TW200510534A (en) * 2002-12-11 2005-03-16 Takara Bio Inc Cold-induced expression vector
WO2005017144A1 (ja) * 2003-08-14 2005-02-24 Takara Bio Inc. ｄｓＲＮＡ分解およびＲＮＡ合成方法
US20070218524A1 (en) * 2003-09-30 2007-09-20 Jun Tomono Polypeptide Having Rnase III Activity
WO2005113768A1 (ja) * 2004-05-21 2005-12-01 Takara Bio Inc. ポリペプチドの製造方法
US8034597B2 (en) 2005-03-08 2011-10-11 Takara Bio Inc. Microorganism-derived psychrophilic endonuclease
US20080307549A1 (en) 2005-08-03 2008-12-11 Adelaide Research & Innovation Pty Ltd. Polysaccharide Synthases
US20090215120A1 (en) * 2006-03-20 2009-08-27 University Of Medicine And Dentistry Of New Jersey The Use of Protein S Fusion for Protein Solubilization
EP2028266A4 (de) 2006-06-09 2010-08-11 Takara Bio Inc Verfahren zur herstellung von lymphozyten
CN101157729B (zh) * 2007-10-23 2011-01-12 南京大学 一种肿瘤坏死因子相关凋亡配体变体及其应用
US9145562B2 (en) * 2009-11-20 2015-09-29 Alberta Innovates—Technology Futures Variegation in plants

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990009447A1 (en) * 1989-02-13 1990-08-23 University Of Medicine And Dentistry Of New Jersey Recombinant cold shock protein, production and use in agriculture
US5714575A (en) 1989-02-13 1998-02-03 The University Of Medicine And Dentistry Of New Jersey Nucleic acids sequence, stress-induced proteins and uses thereof
US5981280A (en) * 1996-03-22 1999-11-09 The University Of Medicine And Denistry Of New Jersey Method and constructs for inhibiting protein expression in bacteria
US5726039A (en) 1994-07-21 1998-03-10 Yissum Research Development Co. Of The Hebrew University Of Jerusalem Vectors and transformed host cells for recombinant protein production at reduced temperatures
US5654169A (en) * 1994-07-21 1997-08-05 Yissum Research & Development Company Of The Hebrew University Vectors and transformed host cells for recombinant protein production at reduced temperatures
KR100596071B1 (ko) 1997-11-20 2006-07-03 다카라 바이오 가부시키가이샤 저온 유도성 발현벡터

Also Published As

Publication number Publication date
DE69838680D1 (de) 2007-12-20
EP1033408B1 (de) 2007-11-07
WO1999027117A1 (en) 1999-06-03
DE69838680T2 (de) 2008-08-28
JP4057237B2 (ja) 2008-03-05
KR20060032223A (ko) 2006-04-14
AU1054699A (en) 1999-06-15
US20030082799A1 (en) 2003-05-01
KR100596071B1 (ko) 2006-07-03
CA2309600C (en) 2009-08-25
EP1033408A1 (de) 2000-09-06
KR20010032249A (ko) 2001-04-16
KR100596070B1 (ko) 2006-07-03
EP1033408A4 (de) 2004-08-25
US6479260B1 (en) 2002-11-12
US6897042B2 (en) 2005-05-24
CA2309600A1 (en) 1999-06-03

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