ATE381595T1 - Verfahren zur optischen bestimmung von mehrsträngigen nucleinsäuren mit hilfe von cyaninfarbstoffen - Google Patents

Verfahren zur optischen bestimmung von mehrsträngigen nucleinsäuren mit hilfe von cyaninfarbstoffen

Info

Publication number
ATE381595T1
ATE381595T1 AT03003488T AT03003488T ATE381595T1 AT E381595 T1 ATE381595 T1 AT E381595T1 AT 03003488 T AT03003488 T AT 03003488T AT 03003488 T AT03003488 T AT 03003488T AT E381595 T1 ATE381595 T1 AT E381595T1
Authority
AT
Austria
Prior art keywords
stranded nucleic
nucleic acid
compound
nucleic acids
cyanine dyes
Prior art date
Application number
AT03003488T
Other languages
English (en)
Inventor
Kouki Nakamura
Kazuya Takeuchi
Original Assignee
Fujifilm Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP2002036474A external-priority patent/JP3899373B2/ja
Priority claimed from JP2002036473A external-priority patent/JP3899372B2/ja
Application filed by Fujifilm Corp filed Critical Fujifilm Corp
Application granted granted Critical
Publication of ATE381595T1 publication Critical patent/ATE381595T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
    • C09B23/04—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups one >CH- group, e.g. cyanines, isocyanines, pseudocyanines
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
    • C09B23/06—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups three >CH- groups, e.g. carbocyanines
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
    • C09B23/08—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups more than three >CH- groups, e.g. polycarbocyanines
    • C09B23/083—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups more than three >CH- groups, e.g. polycarbocyanines five >CH- groups
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B69/00—Dyes not provided for by a single group of this subclass
    • C09B69/001—Dyes containing an onium group attached to the dye skeleton via a bridge
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
AT03003488T 2002-02-14 2003-02-14 Verfahren zur optischen bestimmung von mehrsträngigen nucleinsäuren mit hilfe von cyaninfarbstoffen ATE381595T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2002036474A JP3899373B2 (ja) 2002-02-14 2002-02-14 核酸染色用色素
JP2002036473A JP3899372B2 (ja) 2002-02-14 2002-02-14 多重鎖核酸の光学的測定方法

Publications (1)

Publication Number Publication Date
ATE381595T1 true ATE381595T1 (de) 2008-01-15

Family

ID=29272299

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03003488T ATE381595T1 (de) 2002-02-14 2003-02-14 Verfahren zur optischen bestimmung von mehrsträngigen nucleinsäuren mit hilfe von cyaninfarbstoffen

Country Status (4)

Country Link
US (2) US20050112770A1 (de)
EP (2) EP1362894B1 (de)
AT (1) ATE381595T1 (de)
DE (1) DE60318150T2 (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2005215881B2 (en) * 2004-02-13 2010-11-11 Dsm Ip Assets B.V. Ionic UV-A sunscreens and compositions containing them
US7354720B2 (en) * 2004-12-30 2008-04-08 Affymetrix, Inc. Label free analysis of nucleic acids
WO2012039422A1 (ja) * 2010-09-22 2012-03-29 株式会社カネカ 核酸の検出方法及びデバイス、キット
WO2012121225A1 (ja) 2011-03-04 2012-09-13 株式会社カネカ 核酸の検出方法及びその方法に用いるデバイス、キット
US10260089B2 (en) 2012-10-29 2019-04-16 The Research Foundation Of The State University Of New York Compositions and methods for recognition of RNA using triple helical peptide nucleic acids

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19818176A1 (de) * 1998-04-23 1999-10-28 Basf Ag Verfahren zur Markierung von Flüssigkeiten mit mindestens zwei Markierstoffen und Verfahren zu deren Detektion
DE518291C (de) 1927-01-15 1931-02-18 Schering Kahlbaum Ag Verfahren zur Darstellung von 4-Alkylchinolinen und deren Derivaten
GB870753A (en) * 1957-07-04 1961-06-21 Ilford Ltd Improvements in or relating to methine dyes
US4313951A (en) 1979-11-26 1982-02-02 Sterling Drug Inc. 3-Substituted-6-(lower-alkyl)-5-(pyridinyl)-2(1H)-pyridinones, their cardiotonic use and intermediates therefor
NZ196573A (en) 1980-03-28 1984-03-16 Sterling Drug Inc 6-pyrid-(3 or 4)-yl-(1h or 3h)-imidazo (4,5-b)pyridines
JP2573443B2 (ja) 1990-09-28 1997-01-22 株式会社東芝 遺伝子検出法
US5321130A (en) * 1992-02-10 1994-06-14 Molecular Probes, Inc. Unsymmetrical cyanine dyes with a cationic side chain
US5410030A (en) * 1993-04-05 1995-04-25 Molecular Probes, Inc. Dimers of unsymmetrical cyanine dyes containing pyridinium moieties
US5658751A (en) * 1993-04-13 1997-08-19 Molecular Probes, Inc. Substituted unsymmetrical cyanine dyes with selected permeability
US5656449A (en) * 1995-03-06 1997-08-12 Molecular Probes, Inc. Neutral unsymmetrical cyanine dyes
US6664047B1 (en) * 1999-04-30 2003-12-16 Molecular Probes, Inc. Aza-benzazolium containing cyanine dyes
JP3342695B2 (ja) 1999-12-06 2002-11-11 富士写真フイルム株式会社 反応性固相担体及びｄｎａ断片検出用具
JP2001163895A (ja) 1999-12-07 2001-06-19 Fuji Photo Film Co Ltd 蛍光ヌクレオチド
JP2001270864A (ja) 2000-03-27 2001-10-02 Fuji Photo Film Co Ltd インドレニン誘導体の製造方法
JP2001270957A (ja) 2000-03-27 2001-10-02 Jsr Corp スポンジ用ゴム組成物およびスポンジ

Also Published As

Publication number Publication date
EP1889880A3 (de) 2008-06-18
EP1889880A2 (de) 2008-02-20
DE60318150T2 (de) 2008-12-04
EP1362894B1 (de) 2007-12-19
US20080124732A1 (en) 2008-05-29
EP1362894A3 (de) 2004-03-10
DE60318150D1 (de) 2008-01-31
EP1362894A2 (de) 2003-11-19
US20050112770A1 (en) 2005-05-26

Similar Documents

Publication Publication Date Title
ATE243759T1 (de) Verfahren zur dämpfung der fluoreszene in lösung von fluoreszenzmarkierten oligonucleotidsonden
ATE377093T1 (de) Sequenzbestimmung in echtzeit
ATE148788T1 (de) Verfahren zur aktivierung von polyanionischen,fluoreszierenden farbstoffen in schwach dielektrischen medien mit quaternären oniumverbindungen
DE60042738D1 (de) Verfahren zum nachweis von analyten mit hilfe von halbleiternanokrystallen
FI900602A0 (fi) Bestaemning.
DE60218268D1 (de) Verfahren zur Analyse der Blutkoagluationsreaktion
EP1046717A3 (de) Verfahren und Sonden zur Bestimmung der Konzentration von Nukleinsäure-Molekülen und Verfahren zur Analyse der gewonnenen Daten
DK1042508T3 (da) Fremgangsmåder til forbedrede luminescensassays under anvendelse af mikropartikler
DE69333991D1 (de) Verfahren und Sonde zum Nachweis von Nukleinsäuren
DE3486478D1 (de) Zusammensetzung zur Anwendung in einem Bestimmungsverfahren unter Verwendung von Polynukleotidsequenzen
DK0407789T3 (da) Fremgangsmåde til detektering af nukleinsyrer
DE60115205D1 (de) Nitroreduktase reporter-gen assay mit fluoreszenz erhöhung
ATE373235T1 (de) Fluoreszensanalyseelement mit der anwendung eines metallischen nanolochs und verfahren zur herstellung desselben
DE60213256D1 (de) Verfahren zur bestimmung von mehrere analyten
EP1489418A4 (de) Neue fluoreszenzmarkierungsverbindungen
WO2004062338A3 (en) Method for determining the amount of template nucleic acid present in a sample
ATE219148T1 (de) Methode zum nachweis von bioindividuen mittels einer nukleinsäureprobe nicht-natürlichen typs und probe zur verwendung hierin
DE60318150D1 (de) Verfahren zur optischen Bestimmung von mehrsträngigen Nucleinsäuren mit Hilfe von Cyaninfarbstoffen
EP1321520A4 (de) Neuartige nukleinsäuresonde und neuartiges verfahren zur analyse von nukleinsäuren unter verwendung derselben
ATE532071T1 (de) Verfahren zur verminderung von eiweiss in proben
ATE464756T1 (de) Verfahren zur bereitstellung von ortsinformationen
SE8801011L (sv) Foerfarande foer bestaemning av koncentrationen av en luminiscerande
DE60139333D1 (de) Verfahren zum nachweis von norwalk-ähnlichem virus (gii)
DE60142066D1 (de) Verfahren zum nachweis von norwalk-ähnlichem virus (gi)
EA200000279A1 (ru) Способ повышения оптической чистоты 2r-[1-гидрокси-1-трифторметил-3-циклопропилпропин-2-ил]-4-хлоранилина

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties