ATE384T1 - PROCESS FOR THE PREPARATION OF FIBRINOGEN, A PROTHROMBIN COMPLEX CONTAINING COLOTTING FACTORS II, VII, IX AND X, ANTITHROMBIN III AND A SOLUTION OF STABLE SERUM PROTEINS. - Google Patents

PROCESS FOR THE PREPARATION OF FIBRINOGEN, A PROTHROMBIN COMPLEX CONTAINING COLOTTING FACTORS II, VII, IX AND X, ANTITHROMBIN III AND A SOLUTION OF STABLE SERUM PROTEINS.

Info

Publication number
ATE384T1
ATE384T1 AT80100164T AT80100164T ATE384T1 AT E384 T1 ATE384 T1 AT E384T1 AT 80100164 T AT80100164 T AT 80100164T AT 80100164 T AT80100164 T AT 80100164T AT E384 T1 ATE384 T1 AT E384T1
Authority
AT
Austria
Prior art keywords
antithrombin iii
vii
solution
fibrinogen
adsorbed
Prior art date
Application number
AT80100164T
Other languages
German (de)
Inventor
Ronald Dr.Phil.Nat. Dipl. Kotitschke
Wolfgang Dr.Phil.Nat. Dipl.- Stephan
Original Assignee
Biotest-Serum-Institut Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biotest-Serum-Institut Gmbh filed Critical Biotest-Serum-Institut Gmbh
Application granted granted Critical
Publication of ATE384T1 publication Critical patent/ATE384T1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6429—Thrombin (3.4.21.5)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C07K14/75—Fibrinogen
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C07K14/8121—Serpins
    • C07K14/8128—Antithrombin III
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21005—Thrombin (3.4.21.5)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/827—Proteins from mammals or birds
    • Y10S530/829—Blood
    • Y10S530/83—Plasma; serum

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention relates to a method for making fibrinogen, a prothrombin complex containing the coagulation factors II, VII, IX and X that can contain antithrombin III, antithrombin III and a solution of stable serum proteins from a blood plasma stabilized with citrate which is characterized in that from the plasma, by adsorption on colloidal silica of a specific surface of 50 to 400 m2/g and a concentration of 50 to 400 mg per g plasma protein, fibrinogen is isolated; and in that thereupon (a) citrate and calcium ions are removed by ultrafiltration or dialysis and then from the protein solution, over anion exchangers or tricalcium phosphate that adsorb proteins, the coagulation factors II, VII, IX and X and antithrombin III are adsorbed, or (b) the coagulation factors II, VII, IX and X are adsorbed prior to the ultrafiltration or dialysis, antithrombin III then not being simultaneously adsorbed, and antithrombin III is adsorbed on the said adsorbents after the removal of the citrate and calcium ions by ultrafiltration or dialysis; and in that thereupon from the remaining plasma fluid further unstable proteins are removed by another adsorption on colloidal silica and a solution of stable serum proteins is obtained.
AT80100164T 1979-01-20 1980-01-14 PROCESS FOR THE PREPARATION OF FIBRINOGEN, A PROTHROMBIN COMPLEX CONTAINING COLOTTING FACTORS II, VII, IX AND X, ANTITHROMBIN III AND A SOLUTION OF STABLE SERUM PROTEINS. ATE384T1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19792902158 DE2902158A1 (en) 1979-01-20 1979-01-20 METHOD FOR THE PRODUCTION OF FIBRINOGEN, A PROTHROMINE BIN COMPLEX CONTAINING THE COAGINING FACTORS II, VII, IX AND X, AND ANTITHROMINE III, AND A SOLUTION OF STORAGE-STABLE SERUM PROTEINS
EP80100164A EP0014333B1 (en) 1979-01-20 1980-01-14 Process for the preparation of fibrinogene, a prothrombine complex containing the coagulation factors ii, vii, ix, and x, antithrombine iii, and a solution containing storable serum proteins

Publications (1)

Publication Number Publication Date
ATE384T1 true ATE384T1 (en) 1981-11-15

Family

ID=6061004

Family Applications (1)

Application Number Title Priority Date Filing Date
AT80100164T ATE384T1 (en) 1979-01-20 1980-01-14 PROCESS FOR THE PREPARATION OF FIBRINOGEN, A PROTHROMBIN COMPLEX CONTAINING COLOTTING FACTORS II, VII, IX AND X, ANTITHROMBIN III AND A SOLUTION OF STABLE SERUM PROTEINS.

Country Status (5)

Country Link
US (1) US4272523A (en)
EP (1) EP0014333B1 (en)
JP (1) JPS55100319A (en)
AT (1) ATE384T1 (en)
DE (2) DE2902158A1 (en)

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WO1981002105A1 (en) * 1980-01-28 1981-08-06 Baxter Travenol Lab Therapeutic compositions & methods for manufacture and use
US4404132A (en) * 1980-05-27 1983-09-13 Cutter Laboratories, Inc. Blood coagulation promoting product
US4361510A (en) * 1980-05-27 1982-11-30 Cutter Laboratories, Inc. Blood coagulation promoting product
US4391746A (en) * 1980-05-27 1983-07-05 Cutter Laboratories, Inc. Blood-coagulation-promoting products and methods of preparing them
DE3172210D1 (en) * 1980-05-27 1985-10-17 Miles Lab Blood coagulation promoting product and process of preparing same
US4364861A (en) * 1980-05-27 1982-12-21 Cutter Laboratories, Inc. Blood-coagulation-promoting products and methods of preparing them
DE3033932C2 (en) * 1980-09-10 1984-05-24 Biotest-Serum-Institut Gmbh, 6000 Frankfurt Process for the cold sterilization of preparations containing blood coagulation factor VIII
US4412985A (en) * 1980-10-06 1983-11-01 Edward Shanbrom Depyrogenation process
DE3101752A1 (en) * 1981-01-21 1982-08-26 Behringwerke Ag, 3550 Marburg "METHOD FOR PURIFYING THE BLOOD COAGINING FACTORS II, VII, IX AND / OR X AND PREPARATIONS PRODUCED THEREFORE"
US4459288A (en) * 1981-05-11 1984-07-10 Baxter Travenol Laboratories, Inc. Therapeutic blood clotting factor compositions and their use
US4391801A (en) * 1981-10-29 1983-07-05 Cutter Laboratories, Inc. Plasma protein fraction substantially free of acetate ions
DE3247150A1 (en) * 1982-12-21 1984-06-28 Biotest-Serum-Institut Gmbh, 6000 Frankfurt CLOTHING ACTIVE PLASMA PROTEIN REDUCTION, METHOD FOR THE PRODUCTION THEREOF AND THEIR USE FOR TREATING DISORDERS OF THE HAEMOSTASE SYSTEM
US5165938A (en) * 1984-11-29 1992-11-24 Regents Of The University Of Minnesota Wound healing agents derived from platelets
DE3612137A1 (en) * 1986-04-10 1987-10-15 Biotest Pharma Gmbh STERILE PLASMA REPLACEMENT
JPS6317899A (en) * 1986-07-09 1988-01-25 Green Cross Corp:The Purification of haptoglobin
US4725673A (en) * 1986-08-29 1988-02-16 Alpha Therapeutic Corporation Plasma fraction purification using silica resin bound to a ligand
DE3734923C1 (en) * 1987-10-15 1989-01-26 Biotest Pharma Gmbh Process for the preparation of a sterile plasma protein solution containing fibrinogen and coagulation factor XIII
CA1341379C (en) * 1988-04-28 2002-07-23 Welfide Corporation Purified antithrombin-iii and methods of producing the same
US4963265A (en) * 1988-05-06 1990-10-16 Applied Immunesciences, Inc. Plasma processing device with anaphylatoxin remover
DE3843126C3 (en) * 1988-12-22 1994-10-06 Octapharma Ag Process for the production of a high-purity thrombin concentrate
DE3904354A1 (en) * 1989-02-14 1990-08-16 Behringwerke Ag PASTEURIZED, PURIFIED OF WILLEBRAND FACTOR CONCENTRATE AND METHOD FOR THE PRODUCTION THEREOF
DE3926034C3 (en) * 1989-08-07 1996-11-21 Behringwerke Ag Process for the preparation of a stable factor VIII
AT398079B (en) * 1991-11-04 1994-09-26 Immuno Ag PREPARATION WITH THROMBINE ACTIVITY AND METHOD FOR THEIR PRODUCTION
DE4142908C2 (en) * 1991-12-24 1994-02-10 Octapharma Ag Glarus Method for producing a virus-inactivated prothrombin complex concentrate (PPSB)
DE4202667C1 (en) * 1992-01-29 1993-05-13 Behringwerke Ag, 3550 Marburg, De
US5659017A (en) * 1995-11-07 1997-08-19 Alpha Therapeutic Corporation Anion exchange process for the purification of Factor VIII
US20080199513A1 (en) * 1997-06-24 2008-08-21 Cascade Medical Enterprises, Llc Systems and methods for preparing autologous fibrin glue
US7745106B2 (en) * 1997-06-24 2010-06-29 Cascade Medical Enterprises, Llc Methods and devices for separating liquid components
US6979307B2 (en) * 1997-06-24 2005-12-27 Cascade Medical Enterprises Llc Systems and methods for preparing autologous fibrin glue
MXPA00000009A (en) 1998-06-22 2005-09-08 Autologous Wound Therapy Inc Enriched platelet wound healant.
US6858147B2 (en) * 2001-08-03 2005-02-22 Dispersion Technology, Inc. Method for the removal of heavy metals from aqueous solution by means of silica as an adsorbent in counter-flow selective dialysis
DE102004009400A1 (en) 2004-02-24 2005-09-08 Zlb Behring Gmbh Fibrinogen purification
US20060019234A1 (en) * 2004-07-22 2006-01-26 Shanbrom Technologies, Llc Modern blood banking employing improved cell preservation composition
EP1890820A4 (en) * 2005-03-22 2012-04-11 Cascade Medical Entpr Llc Systems and methods of producing membranes
EP2337793A4 (en) * 2008-10-08 2013-04-10 Thrombodyne Inc Methods of making concentrated fibrinogen- and platelet containing compositions
FR2946348B1 (en) 2009-06-05 2011-08-05 Lab Francais Du Fractionnement PROCESS FOR PREPARING A HIGH-DEPTH PURITY PROTHROMBIC COMPLEX COMPOSITION
AU2010202125B1 (en) 2010-05-26 2010-09-02 Takeda Pharmaceutical Company Limited A method to produce an immunoglobulin preparation with improved yield
US8772462B2 (en) 2010-05-26 2014-07-08 Baxter International Inc. Removal of serine proteases by treatment with finely divided silicon dioxide
US20220380439A1 (en) * 2020-11-09 2022-12-01 Takeda Pharmaceutical Company Limited Purification of fviii from plasma using silicon oxide adsorption
EP4518875A1 (en) * 2022-05-02 2025-03-12 Takeda Pharmaceutical Company Limited Methods of preparing cohn pool concentrate from blood plasma through ultrafiltration

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DE1617335B2 (en) * 1966-11-05 1977-02-24 Biotest-Serum-Institut Gmbh, 6000 Frankfurt PROCESS FOR MANUFACTURING LIPOPROTEIN-FREE, STABLE AND STERILE SERUM
US3652530A (en) * 1967-08-28 1972-03-28 American Nat Red Cross Antihemophilic factor prepared from blood plasma using polyethylene glycol
US3916026A (en) * 1968-09-19 1975-10-28 Biotest Serum Institut Gmbh Method for the preparation of gamma-globulin suitable for intravenous use
US3560475A (en) * 1969-06-19 1971-02-02 Baxter Laboratories Inc Prothrombin complex prepared by precipitation with polyethylene glycol
US3682881A (en) * 1970-10-02 1972-08-08 Baxter Laboratories Inc Fractionation of plasma using glycine and polyethylene glycol
US3869436A (en) * 1971-06-01 1975-03-04 Statens Bakteriologiska Lab Method for fractionating plasma proteins using peg and ion-exchangers
US3920625A (en) * 1973-06-19 1975-11-18 Kabi Ab Isolation of coagulation factors from biological material using cross linked sulfated, sulfonated carbohydrates
US3973002A (en) * 1974-04-12 1976-08-03 E. R. Squibb & Sons, Inc. Antihemophilic factor
DE2459291C3 (en) * 1974-12-14 1981-06-04 Biotest-Serum-Institut Gmbh, 6000 Frankfurt Process for the production of a concentrate containing antihemophilic globulin A in addition to a prothrombin complex and a solution of storage-stable serum proteins
US4087415A (en) * 1976-06-09 1978-05-02 William L. Wilson Antithrombin III
AT350726B (en) * 1976-08-30 1979-06-11 Immuno Ag PROCESS FOR THE PRODUCTION OF A BLOOD CLOTIFYING PREPARATION FROM HUMAN BLOOD PLASMA

Also Published As

Publication number Publication date
US4272523A (en) 1981-06-09
DE2902158A1 (en) 1980-07-31
DE3060075D1 (en) 1982-01-14
EP0014333A1 (en) 1980-08-20
JPS55100319A (en) 1980-07-31
EP0014333B1 (en) 1981-11-11

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