ATE95189T1 - PROCEDURE FOR PURIFICATION OF ANNEXINS. - Google Patents

PROCEDURE FOR PURIFICATION OF ANNEXINS.

Info

Publication number
ATE95189T1
ATE95189T1 AT90113187T AT90113187T ATE95189T1 AT E95189 T1 ATE95189 T1 AT E95189T1 AT 90113187 T AT90113187 T AT 90113187T AT 90113187 T AT90113187 T AT 90113187T AT E95189 T1 ATE95189 T1 AT E95189T1
Authority
AT
Austria
Prior art keywords
annexines
cell residue
chelating agent
homogenized
washing
Prior art date
Application number
AT90113187T
Other languages
German (de)
Inventor
Christiaan Dr Reutelingsperger
Gerhard Prof-Dr Bodo
Original Assignee
Boehringer Ingelheim Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim Int filed Critical Boehringer Ingelheim Int
Application granted granted Critical
Publication of ATE95189T1 publication Critical patent/ATE95189T1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4721—Lipocortins

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Zoology (AREA)
  • Toxicology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Detergent Compositions (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)
  • Amplifiers (AREA)
  • Bidet-Like Cleaning Device And Other Flush Toilet Accessories (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
  • Steroid Compounds (AREA)

Abstract

The present invention relates to processes for purifying annexines. The overall process includes the steps of preparing a homogenized cell preparation and adjusting the pH to about 8.0 to 10.0, adding at least one bivalent cation, adding a phospholipid, washing the insoluble cell residue to remove the soluble constituents, and extracting the annexines from the cell residue with a chelating agent. This process initially promotes the adsorption of the annexines onto the insoluble cell residue or membrane of the host organism. The adsorption step makes it possible to eliminate unwanted soluble matter from the homogenized material by simple washing. Desorption is accomplished by using a chelating agent.
AT90113187T 1989-07-15 1990-07-11 PROCEDURE FOR PURIFICATION OF ANNEXINS. ATE95189T1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE3923501 1989-07-15
EP90113187A EP0409053B1 (en) 1989-07-15 1990-07-11 Process for purifying annexins

Publications (1)

Publication Number Publication Date
ATE95189T1 true ATE95189T1 (en) 1993-10-15

Family

ID=6385155

Family Applications (1)

Application Number Title Priority Date Filing Date
AT90113187T ATE95189T1 (en) 1989-07-15 1990-07-11 PROCEDURE FOR PURIFICATION OF ANNEXINS.

Country Status (18)

Country Link
US (1) US5258497A (en)
EP (1) EP0409053B1 (en)
JP (1) JPH03175992A (en)
KR (1) KR0159275B1 (en)
AT (1) ATE95189T1 (en)
AU (1) AU628604B2 (en)
CA (1) CA2021147A1 (en)
DD (1) DD297977A5 (en)
DE (2) DE4021979A1 (en)
DK (1) DK0409053T3 (en)
ES (1) ES2045669T3 (en)
FI (1) FI95136C (en)
IE (1) IE65195B1 (en)
IL (1) IL95082A (en)
NO (1) NO177787C (en)
NZ (1) NZ234492A (en)
PT (1) PT94674B (en)
ZA (1) ZA905490B (en)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4022010A1 (en) * 1989-07-15 1991-01-17 Boehringer Ingelheim Int Anticoagulant compsn. for treatment and prophylaxis of thrombosis - contains vascular anti-coagulant protein (annexine) and divalent salt of calcium, cadmium, zinc, manganese or cobalt
US5627036A (en) * 1989-12-27 1997-05-06 Boehringer Ingelheim International Gmbh Use of an anticoagulant as a diagnostic agent
DE9110757U1 (en) * 1991-08-30 1992-02-13 Klein, Rainer, 5840 Schwerte Integrated optical fabric sensor
WO1993006230A1 (en) * 1991-09-23 1993-04-01 The General Hospital Corporation Annexin xi
JP3664727B2 (en) * 1994-01-24 2005-06-29 ネオルクス コーポレイション Radiolabeled annexin
US20030220233A1 (en) 1994-01-24 2003-11-27 Neorx Corporation Radiolabeled annexins
US5968477A (en) * 1994-01-24 1999-10-19 Neorx Corporation Radiolabeled annexin conjugates with hexose and a chelator
DE69533366T2 (en) * 1994-12-07 2005-08-04 Neorx Corp., Seattle RADIOACTIVELY MARKED ANNEXIN GALACTOS-CLUSTER CONJUGATES
US6204035B1 (en) 1996-04-02 2001-03-20 The Blood Center Research Foundation Methods and compositions to alter the cell surface expression of phosphatidylserine and other clot-promoting plasma membrane phospholipids
US6172210B1 (en) 1996-04-02 2001-01-09 Blood Center Research Foundation DNA encoding phospholipid scramblase
CN1250567C (en) * 2000-07-21 2006-04-12 财团法人化学及血清疗法研究所 Method of purifying calcium ion-binding protein
US7635676B2 (en) * 2001-02-21 2009-12-22 Alavita Pharmaccuticals, Inc. Modified annexin proteins and methods for their use in organ transplantation
US7635680B2 (en) * 2001-02-21 2009-12-22 Alavita Pharmaceuticals, Inc. Attenuation of reperfusion injury
US20050222030A1 (en) * 2001-02-21 2005-10-06 Anthony Allison Modified annexin proteins and methods for preventing thrombosis
AU2002252035A1 (en) * 2001-02-21 2002-09-12 Surromed, Inc. Modified annexin proteins and methods for preventing thrombosis
US7645739B2 (en) * 2001-02-21 2010-01-12 Alavita Pharmaceuticals, Inc. Modified annexin compositions and methods of using same
US20090291086A1 (en) * 2001-02-21 2009-11-26 Alavita Pharmaceuticals, Inc. Compositions and Methods for Treating Cerebral Thrombosis and Global Cerebral Ischemia
DE10162434A1 (en) * 2001-12-18 2003-09-25 November Ag Molekulare Medizin Expression vector and method for producing an expression vector
AU2003237870A1 (en) * 2002-06-07 2003-12-22 The Brigham & Women's Hospital, Inc. Method and composition for inhibing or slowing blood coagulation
US7771956B2 (en) * 2003-06-30 2010-08-10 Brigham & Women's Hospital, Inc. Method for detecting the presence of a phospholipid
KR102597544B1 (en) * 2021-04-02 2023-11-03 주식회사 디에스시동탄 Support driver and support assembly including the same

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3315000A1 (en) * 1983-04-26 1984-10-31 Behringwerke Ag, 3550 Marburg TISSUE PROTEIN PP (DOWN ARROW) 4 (DOWN ARROW), METHOD FOR ITS RECOVERY AND USE
KR930007434B1 (en) * 1984-09-21 1993-08-10 베링거 잉겔하임 인터내셔날 게엠베하 Manufacturing method of anticoagulant protein
US4683294A (en) * 1985-04-03 1987-07-28 Smith Kline Rit, S.A. Process for the extraction and purification of proteins from culture media producing them
US4937324A (en) * 1987-02-06 1990-06-26 Zymogenetics, Inc. Chromatographic purification of human proteins having anticoagulant and anti-inflammatory activity

Also Published As

Publication number Publication date
KR910002893A (en) 1991-02-26
IL95082A (en) 1995-03-30
DK0409053T3 (en) 1993-11-08
DE4021979A1 (en) 1991-01-31
ZA905490B (en) 1992-03-25
NZ234492A (en) 1991-10-25
KR0159275B1 (en) 1998-11-16
US5258497A (en) 1993-11-02
EP0409053B1 (en) 1993-09-29
FI903508A0 (en) 1990-07-11
PT94674A (en) 1991-04-18
NO903149D0 (en) 1990-07-13
NO177787C (en) 1995-11-22
EP0409053A1 (en) 1991-01-23
PT94674B (en) 1997-03-31
IE902563A1 (en) 1991-02-27
FI95136C (en) 1995-12-27
AU5901590A (en) 1991-01-17
DD297977A5 (en) 1992-01-30
IE65195B1 (en) 1995-10-04
JPH03175992A (en) 1991-07-31
CA2021147A1 (en) 1991-01-16
DE59002895D1 (en) 1993-11-04
IL95082A0 (en) 1991-06-10
NO903149L (en) 1991-01-16
FI95136B (en) 1995-09-15
NO177787B (en) 1995-08-14
ES2045669T3 (en) 1994-01-16
AU628604B2 (en) 1992-09-17

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Legal Events

Date Code Title Description
EELA Cancelled due to lapse of time