ATE386126T1 - Verbesserte expressionsvektoren - Google Patents

Verbesserte expressionsvektoren

Info

Publication number
ATE386126T1
ATE386126T1 AT97947444T AT97947444T ATE386126T1 AT E386126 T1 ATE386126 T1 AT E386126T1 AT 97947444 T AT97947444 T AT 97947444T AT 97947444 T AT97947444 T AT 97947444T AT E386126 T1 ATE386126 T1 AT E386126T1
Authority
AT
Austria
Prior art keywords
expression vectors
improved expression
nucleic acid
constructs
encodes
Prior art date
Application number
AT97947444T
Other languages
English (en)
Inventor
Jody Schultz
Gary Hermanson
Original Assignee
Neose Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Neose Technologies Inc filed Critical Neose Technologies Inc
Application granted granted Critical
Publication of ATE386126T1 publication Critical patent/ATE386126T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/72—Expression systems using regulatory sequences derived from the lac-operon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/582—Recycling of unreacted starting or intermediate materials

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Pulse, Heart Rate, Blood Pressure Or Blood Flow (AREA)
  • Measurement And Recording Of Electrical Phenomena And Electrical Characteristics Of The Living Body (AREA)
  • Image Generation (AREA)
AT97947444T 1996-11-08 1997-11-07 Verbesserte expressionsvektoren ATE386126T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US2954596P 1996-11-08 1996-11-08

Publications (1)

Publication Number Publication Date
ATE386126T1 true ATE386126T1 (de) 2008-03-15

Family

ID=21849585

Family Applications (1)

Application Number Title Priority Date Filing Date
AT97947444T ATE386126T1 (de) 1996-11-08 1997-11-07 Verbesserte expressionsvektoren

Country Status (13)

Country Link
US (1) US6117651A (de)
EP (1) EP0946711B1 (de)
JP (1) JP2001503274A (de)
KR (1) KR20000068934A (de)
CN (1) CN1244214A (de)
AT (1) ATE386126T1 (de)
AU (1) AU718382B2 (de)
CA (1) CA2271230A1 (de)
DE (1) DE69738514D1 (de)
HU (1) HUP0001650A3 (de)
IL (1) IL129843A0 (de)
NZ (1) NZ335628A (de)
WO (1) WO1998020111A1 (de)

Families Citing this family (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1360310A2 (de) * 2001-02-13 2003-11-12 University Of Florida Bidirektionaler dualer promotorkomplex mit verstärkter promotoraktivität zur transgenen expression in eukaroynten
US7172865B2 (en) * 2001-08-13 2007-02-06 Saint Louis University Rapid and sensitive assay for the detection and quantification of coregulators of nucleic acid binding factors
US7157277B2 (en) 2001-11-28 2007-01-02 Neose Technologies, Inc. Factor VIII remodeling and glycoconjugation of Factor VIII
US8008252B2 (en) 2001-10-10 2011-08-30 Novo Nordisk A/S Factor VII: remodeling and glycoconjugation of Factor VII
US7214660B2 (en) 2001-10-10 2007-05-08 Neose Technologies, Inc. Erythropoietin: remodeling and glycoconjugation of erythropoietin
US7173003B2 (en) 2001-10-10 2007-02-06 Neose Technologies, Inc. Granulocyte colony stimulating factor: remodeling and glycoconjugation of G-CSF
CN1314810C (zh) * 2002-01-31 2007-05-09 圣路易大学 一种用来检测和定量核酸结合因子辅调节物的快速灵敏测定法
DE60336555D1 (de) 2002-06-21 2011-05-12 Novo Nordisk Healthcare Ag Pegylierte glykoformen von faktor vii
EP1539794B1 (de) * 2002-07-03 2008-12-31 Dow Global Technologies Inc. Benzoat- und antranilatinduzierbare promotoren
ATE506450T1 (de) 2002-07-23 2011-05-15 Biogenerix Ag Synthese von oligosacchariden, glykolipiden und glykoproteinen unter verwendung bakterieller glykosyltransferasen
NZ542094A (en) 2003-03-14 2008-12-24 Neose Technologies Inc Branched polymer conjugates comprising a peptide and water-soluble polymer chains
PL1615945T3 (pl) 2003-04-09 2012-03-30 Ratiopharm Gmbh Sposoby glikopegylacji i białka/peptydy wytwarzane tymi sposobami
US8791070B2 (en) 2003-04-09 2014-07-29 Novo Nordisk A/S Glycopegylated factor IX
JP2007523630A (ja) 2003-05-09 2007-08-23 ネオス テクノロジーズ インコーポレイテッド ヒト成長ホルモングリコシル化突然変異体の組成と調合法
US9005625B2 (en) 2003-07-25 2015-04-14 Novo Nordisk A/S Antibody toxin conjugates
WO2005040388A2 (en) * 2003-08-22 2005-05-06 Nucleonics Inc. Eukariotic expression systems for expression of inhibitory rna in multiple intracellular compartments
US8633157B2 (en) 2003-11-24 2014-01-21 Novo Nordisk A/S Glycopegylated erythropoietin
US7842661B2 (en) 2003-11-24 2010-11-30 Novo Nordisk A/S Glycopegylated erythropoietin formulations
US20080305992A1 (en) 2003-11-24 2008-12-11 Neose Technologies, Inc. Glycopegylated erythropoietin
JP2007515410A (ja) * 2003-12-03 2007-06-14 ネオス テクノロジーズ インコーポレイテッド ＧｌｙｃｏＰＥＧ化卵胞刺激ホルモン
US7956032B2 (en) 2003-12-03 2011-06-07 Novo Nordisk A/S Glycopegylated granulocyte colony stimulating factor
US20060040856A1 (en) 2003-12-03 2006-02-23 Neose Technologies, Inc. Glycopegylated factor IX
NZ548123A (en) 2004-01-08 2010-05-28 Novo Nordisk As O-linked glycosylation of peptides
US20080058245A1 (en) * 2004-01-09 2008-03-06 Johnson Karl F Vectors for Recombinant Protein Expression in E. Coli
AU2005222782B2 (en) 2004-02-04 2010-06-17 Ratiopharm Gmbh Methods of refolding mammalian glycosyltransferases
US20080300173A1 (en) 2004-07-13 2008-12-04 Defrees Shawn Branched Peg Remodeling and Glycosylation of Glucagon-Like Peptides-1 [Glp-1]
DE602005024015D1 (de) * 2004-08-23 2010-11-18 Alnylam Pharmaceuticals Inc Expressionskonstrukte mit mehreren rna-polymerase-iii-promotoren
WO2006031811A2 (en) 2004-09-10 2006-03-23 Neose Technologies, Inc. Glycopegylated interferon alpha
WO2006050247A2 (en) 2004-10-29 2006-05-11 Neose Technologies, Inc. Remodeling and glycopegylation of fibroblast growth factor (fgf)
US9029331B2 (en) 2005-01-10 2015-05-12 Novo Nordisk A/S Glycopegylated granulocyte colony stimulating factor
WO2006121569A2 (en) 2005-04-08 2006-11-16 Neose Technologies, Inc. Compositions and methods for the preparation of protease resistant human growth hormone glycosylation mutants
EP2975135A1 (de) 2005-05-25 2016-01-20 Novo Nordisk A/S Glykopegylierter Faktor IX
US20070105755A1 (en) 2005-10-26 2007-05-10 Neose Technologies, Inc. One pot desialylation and glycopegylation of therapeutic peptides
WO2007056191A2 (en) 2005-11-03 2007-05-18 Neose Technologies, Inc. Nucleotide sugar purification using membranes
US8198045B2 (en) 2006-04-19 2012-06-12 Biogenerix Ag Expression of O-glycosylated therapeutic proteins in prokaryotic microorganisms
EP2049144B8 (de) 2006-07-21 2015-02-18 ratiopharm GmbH Glycosylierung von peptiden über o-verbundene glycosylierungssequenzen
EP2054521A4 (de) 2006-10-03 2012-12-19 Novo Nordisk As Verfahren zur reinigung von polypeptid-konjugate
HRP20130382T1 (hr) 2007-04-03 2013-05-31 Biogenerix Ag Postupci lijeäśenja pomoä†u glikopegiliranog g-csf
UA100692C2 (ru) 2007-05-02 2013-01-25 Мериал Лимитед Днк-плазмиды, имеющие повышенную экспрессию и стабильность
CA2690611C (en) 2007-06-12 2015-12-08 Novo Nordisk A/S Improved process for the production of nucleotide sugars
US8207112B2 (en) 2007-08-29 2012-06-26 Biogenerix Ag Liquid formulation of G-CSF conjugate
PL2257311T3 (pl) 2008-02-27 2014-09-30 Novo Nordisk As Koniugaty cząsteczek czynnika VIII
US9616114B1 (en) 2014-09-18 2017-04-11 David Gordon Bermudes Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
EP3938518A4 (de) 2019-03-12 2023-01-11 Terra Bioworks, Inc. Expressionsvektor
US12398397B2 (en) 2022-04-19 2025-08-26 Worcester Polytechnic Institute Inducible promoters

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4565785A (en) * 1978-06-08 1986-01-21 The President And Fellows Of Harvard College Recombinant DNA molecule
US4673641A (en) * 1982-12-16 1987-06-16 Molecular Genetics Research And Development Limited Partnership Co-aggregate purification of proteins
US4710473A (en) * 1983-08-10 1987-12-01 Amgen, Inc. DNA plasmids
US4738921A (en) * 1984-09-27 1988-04-19 Eli Lilly And Company Derivative of the tryptophan operon for expression of fused gene products
US4795706A (en) * 1985-01-31 1989-01-03 Eli Lilly And Company Novel expression control sequences
US5304472A (en) * 1992-11-20 1994-04-19 Genentech, Inc. Method of controlling polypeptide production in bacterial cells
US5342763A (en) * 1992-11-23 1994-08-30 Genentech, Inc. Method for producing polypeptide via bacterial fermentation
US5545553A (en) * 1994-09-26 1996-08-13 The Rockefeller University Glycosyltransferases for biosynthesis of oligosaccharides, and genes encoding them

Also Published As

Publication number Publication date
CN1244214A (zh) 2000-02-09
HUP0001650A2 (hu) 2000-09-28
EP0946711A1 (de) 1999-10-06
KR20000068934A (ko) 2000-11-25
HUP0001650A3 (en) 2001-09-28
WO1998020111A1 (en) 1998-05-14
DE69738514D1 (de) 2008-03-27
US6117651A (en) 2000-09-12
JP2001503274A (ja) 2001-03-13
NZ335628A (en) 2000-10-27
EP0946711B1 (de) 2008-02-13
IL129843A0 (en) 2000-02-29
CA2271230A1 (en) 1998-05-14
EP0946711A4 (de) 2004-10-27
AU718382B2 (en) 2000-04-13
AU5252498A (en) 1998-05-29

Similar Documents

Publication Publication Date Title
ATE386126T1 (de) Verbesserte expressionsvektoren
BR9914278A (pt) Cepa de chrysosporium mutante, construção de ácido nucléico, cepa microbiana recombinante, métodos para produzir um polipeptìdeo de interesse, e para produzir uma cepa de chrysosporium mutante, e, xilanase de chrysosporium da famìlia de xilanases f,
ATE384798T1 (de) Polypeptide mit phytase-aktivität und sie kodierende nukleinsäuren
AU2590901A (en) Il-17 homologous polypeptides and therapeutic uses thereof
WO1998056927A3 (en) Nucleic acids encoding polypeptide having protease activity
WO1999055868A3 (en) Fizz proteins
AP9901541A0 (en) Broad-spectrum delta-endotoxins.
ATE447583T1 (de) Polypeptide und dafür kodierende nukleinsäure
US6114148C1 (en) High level expression of proteins
EP1798239A3 (de) G-Protein-gekoppelte Rezeptoren und deren Verwendung
ATE426024T1 (de) C-c chemokin rezeptor 3: ckr-3 oder eos-l2
ATE414775T1 (de) Polypeptide mit prolyldipeptidylaminopeptidase- aktivität und dafür kodierende nukleinsäuren
EP0963435A4 (de) Verfahren zur herstellung von proteinen
BR0009393A (pt) Polipeptìdeo, sequência de ácido nucleico isolada, construção de ácido nucleico, vetor de expressão recombinante, célula hospedeira recombinante, método para a produção do polipeptìdeo, uso de um polipeptìdeo, e, composição detergente
ATE384787T1 (de) Tripeptidyl-aminopeptidase
DE69837475D1 (de) Polypeptide mit aminopeptidaseaktivität und dafür kodierende nukleinsäuren
CA2252493A1 (en) .beta.-galactoside - .alpha. 2,6-sialyltransferase gene
DK0724637T3 (da) Kortikotropinfrigørende faktor2-receptorer
CA2238662A1 (en) Antioxidant responsive element
ES2134760T3 (es) Polipeptido saf, gen, promotor y uso para la expresion en streptomyces.
WO2001042286A3 (en) Human leucine rich repeat-containing polypeptide and uses therefor
EP1241186A3 (de) Menschliches gas-6 (growth arrest-specific gene 6) Protein und dafür kodierende Nukleinsäure
KR100515855B1 (en) PRO351 Polypeptides
EP1241180A3 (de) Menschliches A33-Antigen-ähnliches Protein und dafür kodierende Nukleinsäure
ECSP993285A (es) Sintesis recombinante de beta lipotropina y otros peptidos

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties