ATE389718T1 - Isolierung von nukleinsäuren unter verwendung eines polykationischen polymers als fällungsmittel - Google Patents
Isolierung von nukleinsäuren unter verwendung eines polykationischen polymers als fällungsmittelInfo
- Publication number
- ATE389718T1 ATE389718T1 AT03761887T AT03761887T ATE389718T1 AT E389718 T1 ATE389718 T1 AT E389718T1 AT 03761887 T AT03761887 T AT 03761887T AT 03761887 T AT03761887 T AT 03761887T AT E389718 T1 ATE389718 T1 AT E389718T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acid
- precipitating agent
- polycationic
- agent
- preciticating
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title abstract 6
- 102000039446 nucleic acids Human genes 0.000 title abstract 6
- 108020004707 nucleic acids Proteins 0.000 title abstract 6
- 239000003795 chemical substances by application Substances 0.000 title abstract 5
- 238000002955 isolation Methods 0.000 title 1
- 229920002851 polycationic polymer Polymers 0.000 title 1
- 230000001376 precipitating effect Effects 0.000 abstract 4
- 238000000034 method Methods 0.000 abstract 2
- 238000001556 precipitation Methods 0.000 abstract 2
- 125000003277 amino group Chemical group 0.000 abstract 1
- 229920000642 polymer Polymers 0.000 abstract 1
- 239000002244 precipitate Substances 0.000 abstract 1
- 150000003839 salts Chemical class 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Saccharide Compounds (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE0202074A SE0202074D0 (sv) | 2002-06-28 | 2002-06-28 | Isolation of nucleic acids |
| SE0301034A SE0301034D0 (sv) | 2002-06-28 | 2003-04-08 | Improved isolation of nucleic acids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE389718T1 true ATE389718T1 (de) | 2008-04-15 |
Family
ID=26655712
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT03761887T ATE389718T1 (de) | 2002-06-28 | 2003-06-26 | Isolierung von nukleinsäuren unter verwendung eines polykationischen polymers als fällungsmittel |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20050222404A1 (de) |
| EP (1) | EP1517990B1 (de) |
| JP (1) | JP2005531329A (de) |
| AT (1) | ATE389718T1 (de) |
| AU (1) | AU2003243108A1 (de) |
| CA (1) | CA2488616A1 (de) |
| DE (1) | DE60319825T2 (de) |
| ES (1) | ES2301823T3 (de) |
| SE (2) | SE0202074D0 (de) |
| WO (1) | WO2004003200A1 (de) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AT501380B1 (de) * | 2005-02-07 | 2007-11-15 | Vitali Dipl Ing Dshemelinski | Verfahren zur reinigung der dna/rna von proteinen und zellulären reststoffen im zellenlysat |
| DE102005015005A1 (de) * | 2005-04-01 | 2006-10-05 | Qiagen Gmbh | Verfahren zur Behandlung einer Biomoleküle enthaltenden Probe |
| US20060234251A1 (en) * | 2005-04-19 | 2006-10-19 | Lumigen, Inc. | Methods of enhancing isolation of RNA from biological samples |
| US9347055B2 (en) | 2007-11-05 | 2016-05-24 | Eiken Kagaku Kabushiki Kaisha | Method and kit for preparation of sample for use in nucleic acid amplification |
| WO2011151428A1 (en) | 2010-06-01 | 2011-12-08 | Qiagen Gmbh | Method for isolating and/or purifying nucleic acid(s) |
| US11648561B2 (en) | 2012-02-13 | 2023-05-16 | Neumodx Molecular, Inc. | System and method for processing and detecting nucleic acids |
| US9604213B2 (en) | 2012-02-13 | 2017-03-28 | Neumodx Molecular, Inc. | System and method for processing and detecting nucleic acids |
| US9738887B2 (en) | 2012-02-13 | 2017-08-22 | Neumodx Molecular, Inc. | Microfluidic cartridge for processing and detecting nucleic acids |
| US9637775B2 (en) | 2012-02-13 | 2017-05-02 | Neumodx Molecular, Inc. | System and method for processing biological samples |
| US11485968B2 (en) | 2012-02-13 | 2022-11-01 | Neumodx Molecular, Inc. | Microfluidic cartridge for processing and detecting nucleic acids |
| WO2014066376A1 (en) | 2012-10-25 | 2014-05-01 | Neumodx Molecular, Inc. | Method and materials for isolation of nucleic acid materials |
| WO2019013991A2 (en) | 2017-07-12 | 2019-01-17 | Illumina, Inc. | METHODS, SYSTEMS AND MATERIALS FOR EXTRACTING NUCLEIC ACIDS |
| CN114133991A (zh) * | 2021-08-27 | 2022-03-04 | 深圳市朗司医疗科技有限公司 | 一种核酸清洗组合试剂及使用方法 |
| WO2025047408A1 (ja) | 2023-09-01 | 2025-03-06 | 栄研化学株式会社 | 核酸精製方法及び核酸精製のためのキット |
| WO2025047407A1 (ja) | 2023-09-01 | 2025-03-06 | 栄研化学株式会社 | 核酸精製方法及び核酸精製のためのキット |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4055469A (en) * | 1976-12-10 | 1977-10-25 | Eastman Kodak Company | Purification of microbial enzyme extracts using synthetic polyelectrolytes |
| EP0281390B1 (de) * | 1987-03-02 | 1994-06-22 | Gen-Probe Incorporated | Polykationische Träger zur Reinigung, Trennung und Hybridisierung von Nukleinsäure |
| US5599667A (en) * | 1987-03-02 | 1997-02-04 | Gen-Probe Incorporated | Polycationic supports and nucleic acid purification separation and hybridization |
| US5010183A (en) * | 1989-07-07 | 1991-04-23 | Macfarlane Donald E | Process for purifying DNA and RNA using cationic detergents |
| US7683035B1 (en) * | 1999-02-23 | 2010-03-23 | Qiagen, Gmbh | Method of stabilizing and/or isolating nucleic acids |
| US20020010145A1 (en) * | 1999-07-12 | 2002-01-24 | Willson Richard C. | Apparatus, methods and compositions for biotechnical separations |
-
2002
- 2002-06-28 SE SE0202074A patent/SE0202074D0/xx unknown
-
2003
- 2003-04-08 SE SE0301034A patent/SE0301034D0/xx unknown
- 2003-06-26 ES ES03761887T patent/ES2301823T3/es not_active Expired - Lifetime
- 2003-06-26 EP EP03761887A patent/EP1517990B1/de not_active Expired - Lifetime
- 2003-06-26 CA CA002488616A patent/CA2488616A1/en not_active Abandoned
- 2003-06-26 DE DE60319825T patent/DE60319825T2/de not_active Expired - Fee Related
- 2003-06-26 JP JP2004548907A patent/JP2005531329A/ja active Pending
- 2003-06-26 AU AU2003243108A patent/AU2003243108A1/en not_active Abandoned
- 2003-06-26 AT AT03761887T patent/ATE389718T1/de not_active IP Right Cessation
- 2003-06-26 WO PCT/SE2003/001127 patent/WO2004003200A1/en not_active Ceased
- 2003-06-26 US US10/517,227 patent/US20050222404A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| AU2003243108A1 (en) | 2004-01-19 |
| EP1517990B1 (de) | 2008-03-19 |
| SE0202074D0 (sv) | 2002-07-02 |
| CA2488616A1 (en) | 2004-01-08 |
| JP2005531329A (ja) | 2005-10-20 |
| SE0301034D0 (sv) | 2003-04-08 |
| EP1517990A1 (de) | 2005-03-30 |
| US20050222404A1 (en) | 2005-10-06 |
| ES2301823T3 (es) | 2008-07-01 |
| DE60319825D1 (de) | 2008-04-30 |
| DE60319825T2 (de) | 2009-03-05 |
| WO2004003200A1 (en) | 2004-01-08 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |