ATE393219T1 - Verfahren zur analyse von nicht-protein komponenten mittels verwendung einer protease aus einem bacillus-stamm - Google Patents

Verfahren zur analyse von nicht-protein komponenten mittels verwendung einer protease aus einem bacillus-stamm

Info

Publication number
ATE393219T1
ATE393219T1 AT01125322T AT01125322T ATE393219T1 AT E393219 T1 ATE393219 T1 AT E393219T1 AT 01125322 T AT01125322 T AT 01125322T AT 01125322 T AT01125322 T AT 01125322T AT E393219 T1 ATE393219 T1 AT E393219T1
Authority
AT
Austria
Prior art keywords
protease
bacillus strain
mixture
proteinaceous
protein components
Prior art date
Application number
AT01125322T
Other languages
English (en)
Inventor
Eberhard Russmann
Rainer Schmuck
Thomas Meier
Johnny Staepels
Uwe Wehnes
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP00123728A external-priority patent/EP1201752A1/de
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of ATE393219T1 publication Critical patent/ATE393219T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21062—Subtilisin (3.4.21.62)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/32—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Bacillus (G)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
AT01125322T 2000-10-31 2001-10-26 Verfahren zur analyse von nicht-protein komponenten mittels verwendung einer protease aus einem bacillus-stamm ATE393219T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP00123728A EP1201752A1 (de) 2000-10-31 2000-10-31 Verfahren zur Analyse von nicht-proteischen Bestandteilen mittels einer Protease aus einem Bacillus-Stamm
EP01106308 2001-03-15

Publications (1)

Publication Number Publication Date
ATE393219T1 true ATE393219T1 (de) 2008-05-15

Family

ID=26071562

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01125322T ATE393219T1 (de) 2000-10-31 2001-10-26 Verfahren zur analyse von nicht-protein komponenten mittels verwendung einer protease aus einem bacillus-stamm

Country Status (6)

Country Link
US (2) US6727067B2 (de)
JP (1) JP3621673B2 (de)
AT (1) ATE393219T1 (de)
AU (1) AU758744B2 (de)
CA (1) CA2360012C (de)
DE (1) DE60133708T2 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2005200670B2 (en) * 2004-02-20 2007-05-03 F. Hoffmann-La Roche Ag Adsorption of nucleic acids to a solid phase
US20060188892A1 (en) * 2005-02-18 2006-08-24 Ambion, Inc. Enzymatic digestion of tissue
US9933445B1 (en) 2016-05-16 2018-04-03 Hound Labs, Inc. System and method for target substance identification
US20200245898A1 (en) 2019-01-31 2020-08-06 Hound Labs, Inc. Single-use Microfluidic Cartridge for Detection of Target Chemical Presence in Human Breath
US11977086B2 (en) 2019-03-21 2024-05-07 Hound Labs, Inc. Biomarker detection from breath samples
US11933731B1 (en) 2020-05-13 2024-03-19 Hound Labs, Inc. Systems and methods using Surface-Enhanced Raman Spectroscopy for detecting tetrahydrocannabinol
US12392769B1 (en) 2021-01-12 2025-08-19 Hound Labs, Inc. Ambient contamination in breath analyte detection and measurement
WO2024254620A2 (en) * 2024-08-01 2024-12-12 Novozymes A/S Protease for diagnostics
CN121347798B (zh) * 2025-12-19 2026-03-24 嘉兴科瑞迪医疗器械有限公司 一种曲霉半乳甘露聚糖的检测用联合试剂、试剂盒及其检测方法

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1234445A (de) * 1967-10-03 1971-06-03
DE3608453A1 (de) * 1986-03-14 1987-09-17 Boehringer Mannheim Gmbh Verfahren zur enzymatischen bestimmung von bilirubin im serum
AU619170B2 (en) * 1987-01-09 1992-01-23 Abbott Laboratories Diagnostic assays using nucleic acid probes
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer
JP3633932B2 (ja) * 1992-04-01 2005-03-30 ザ ジョーンズ ホプキンズ ユニバーシティー スクール オブ メディシン 糞便試料から単離した哺乳類の核酸を検出する方法、およびその検出用試薬
US6403367B1 (en) * 1994-07-07 2002-06-11 Nanogen, Inc. Integrated portable biological detection system
US5834181A (en) * 1994-07-28 1998-11-10 Genzyme Corporation High throughput screening method for sequences or genetic alterations in nucleic acids
US5589330A (en) * 1994-07-28 1996-12-31 Genzyme Corporation High-throughput screening method for sequence or genetic alterations in nucleic acids using elution and sequencing of complementary oligonucleotides
US5849483A (en) * 1994-07-28 1998-12-15 Ig Laboratories, Inc. High throughput screening method for sequences or genetic alterations in nucleic acids
DE4431432A1 (de) 1994-09-03 1996-03-28 Juergen Weidner Isolierung und Amplifizierung von DNA
CN1157553A (zh) * 1994-09-09 1997-08-20 诺沃挪第克公司 清洁、消毒和防护隐形眼镜的方法
JP4044143B2 (ja) 1996-11-04 2008-02-06 ノボザイムス アクティーゼルスカブ ズブチラーゼ変異体及び組成物
AU1705400A (en) * 1998-10-15 2000-05-01 Sarawak Medichem Pharmaceuticals, Inc. Method and composition for treating and preventing tuberculosis
AU2200100A (en) * 1998-12-21 2000-07-12 Genencor International, Inc. Chemically modified enzymes with multiple charged variants
JP2004508011A (ja) * 2000-04-03 2004-03-18 マキシジェン，　インコーポレイテッド スブチリシン変異体

Also Published As

Publication number Publication date
US20030165855A1 (en) 2003-09-04
JP3621673B2 (ja) 2005-02-16
CA2360012C (en) 2008-09-02
DE60133708D1 (de) 2008-06-05
JP2002355096A (ja) 2002-12-10
US6727067B2 (en) 2004-04-27
DE60133708T2 (de) 2009-07-02
US20040063155A1 (en) 2004-04-01
AU758744B2 (en) 2003-03-27
AU8361901A (en) 2002-05-02
CA2360012A1 (en) 2002-04-30

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