ATE394473T1 - FERMENTATION PROCESS WITH LOW CELL DENSITY FOR THE PRODUCTION OF HETEROLOGUE RECOMBINANT PROTEINS IN MICROORGANISMS - Google Patents
FERMENTATION PROCESS WITH LOW CELL DENSITY FOR THE PRODUCTION OF HETEROLOGUE RECOMBINANT PROTEINS IN MICROORGANISMSInfo
- Publication number
- ATE394473T1 ATE394473T1 AT04821402T AT04821402T ATE394473T1 AT E394473 T1 ATE394473 T1 AT E394473T1 AT 04821402 T AT04821402 T AT 04821402T AT 04821402 T AT04821402 T AT 04821402T AT E394473 T1 ATE394473 T1 AT E394473T1
- Authority
- AT
- Austria
- Prior art keywords
- production
- fermentation
- cell density
- feed medium
- microorganisms
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
A low cell density fermentation process for the production of heterologous proteins in microorganisms. The cell culture obtained by cultivating host microorganisms transformed with a vector carrying genetic material for the said proteins and an inducible promoter under batch fermentation conditions is fed with a feed medium after an OD600 of 0.16 to 8 has been achieved or after 0 to 4 hrs from the start of the fermentation process. The feed medium comprises 5 to 30% of carbon source and 1 to 30% of nitrogen source and 0 mg to 400 mg antibiotics and 2.5 to 4.25% inorganic phosphates and trace elements. The concentration of the carbon source in the feed medium is 10 to 30 and the amino acid content in nitrogen source is 45 to 95%. The initial feed rate is in the range of 0 ml/hr to 12 ml/hr and is raised exponentially by an exponent in the range of 0.1 to 0.4 and/or linearly with the slope of the curve in the range of 0.5 to 3. The production of the heterologous proteins is induced with 0.01-4% inducer at a cell density of OD600 0.1-OD600 50. The feeding of the cell culture with the feed medium and the feed rate described above is continued after production has been induced. The pO2 is adjusted between 10% to 60% by passing sterile air into the fermentation broth and the temperature and pH of the fermentation broth are maintained at 33° C.-41° C. and 6.9-8.5, respectively during the entire fermentation.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/IN2004/000393 WO2006067793A1 (en) | 2004-12-21 | 2004-12-21 | Low cell density fermentation process for the production of heterologous recombinant proteins in microorganisms |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE394473T1 true ATE394473T1 (en) | 2008-05-15 |
Family
ID=36601439
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT04821402T ATE394473T1 (en) | 2004-12-21 | 2004-12-21 | FERMENTATION PROCESS WITH LOW CELL DENSITY FOR THE PRODUCTION OF HETEROLOGUE RECOMBINANT PROTEINS IN MICROORGANISMS |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US20100047870A1 (en) |
| EP (1) | EP1828375B1 (en) |
| AT (1) | ATE394473T1 (en) |
| AU (1) | AU2004325822A1 (en) |
| BR (1) | BRPI0419235A (en) |
| CA (1) | CA2589716A1 (en) |
| DE (1) | DE602004013648D1 (en) |
| WO (1) | WO2006067793A1 (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE515566T1 (en) | 2005-05-26 | 2011-07-15 | Cytos Biotechnology Ag | SCALABLE FERMENTATION PROCESS |
| MX339605B (en) | 2010-09-21 | 2016-06-02 | Ferring Bv | Improved process for production of recombinant human growth hormone. |
| KR101602286B1 (en) | 2011-07-13 | 2016-03-10 | 푸드체크 시스템스, 아이엔씨. | Culture medium, method for culturing listeria, and method for detecting listeria |
| CN109504714B (en) * | 2017-09-15 | 2022-04-12 | 北京蓝晶微生物科技有限公司 | A kind of method for producing polyhydroxyalkanoate by fermentation without sterilization |
| CN114015628A (en) * | 2021-12-17 | 2022-02-08 | 河南兴华生物技术有限公司 | Culture medium and culture method for expressing PMT protein |
| CN120699876B (en) * | 2025-08-14 | 2026-03-06 | 天津中合基因科技有限公司 | Culture medium for fermenting terminal deoxynucleotidyl transferase and application thereof |
| CN121227671A (en) * | 2025-09-10 | 2025-12-30 | 江苏卫生健康职业学院 | Fermentation preparation process of an immunoglobulin G degrading enzyme |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5595905A (en) * | 1992-03-12 | 1997-01-21 | G.D. Searle & Co. | Process control system for fed-batch fermentation using a computer to predict nutrient consumption |
| DE4337787A1 (en) * | 1993-11-05 | 1995-05-11 | Hoechst Ag | Oxygen-dependent fermentation of microorganisms |
| SE9904502D0 (en) * | 1999-12-09 | 1999-12-09 | Pharmacia & Upjohn Ab | Production of peptides |
| US6955892B2 (en) * | 2002-11-12 | 2005-10-18 | Akzo Nobel N.V. | Feeding processes for fermentation |
-
2004
- 2004-12-21 WO PCT/IN2004/000393 patent/WO2006067793A1/en not_active Ceased
- 2004-12-21 CA CA002589716A patent/CA2589716A1/en not_active Abandoned
- 2004-12-21 US US11/722,504 patent/US20100047870A1/en not_active Abandoned
- 2004-12-21 BR BRPI0419235-4A patent/BRPI0419235A/en not_active IP Right Cessation
- 2004-12-21 AU AU2004325822A patent/AU2004325822A1/en not_active Abandoned
- 2004-12-21 EP EP04821402A patent/EP1828375B1/en not_active Expired - Lifetime
- 2004-12-21 DE DE602004013648T patent/DE602004013648D1/en not_active Expired - Lifetime
- 2004-12-21 AT AT04821402T patent/ATE394473T1/en not_active IP Right Cessation
-
2011
- 2011-02-07 US US13/022,617 patent/US20110189733A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| AU2004325822A1 (en) | 2006-06-29 |
| BRPI0419235A (en) | 2007-12-18 |
| US20100047870A1 (en) | 2010-02-25 |
| US20110189733A1 (en) | 2011-08-04 |
| EP1828375A1 (en) | 2007-09-05 |
| DE602004013648D1 (en) | 2008-06-19 |
| CA2589716A1 (en) | 2006-06-29 |
| EP1828375B1 (en) | 2008-05-07 |
| WO2006067793A1 (en) | 2006-06-29 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |