ATE400650T1 - Verfahren zur herstellung von nukleinsäuremolekülen - Google Patents
Verfahren zur herstellung von nukleinsäuremolekülenInfo
- Publication number
- ATE400650T1 ATE400650T1 AT02023385T AT02023385T ATE400650T1 AT E400650 T1 ATE400650 T1 AT E400650T1 AT 02023385 T AT02023385 T AT 02023385T AT 02023385 T AT02023385 T AT 02023385T AT E400650 T1 ATE400650 T1 AT E400650T1
- Authority
- AT
- Austria
- Prior art keywords
- oligonucleotide
- recognition site
- stranded
- restriction enzyme
- ligation product
- Prior art date
Links
- 238000004519 manufacturing process Methods 0.000 title abstract 3
- 108020004707 nucleic acids Proteins 0.000 title abstract 3
- 150000007523 nucleic acids Chemical class 0.000 title abstract 3
- 102000039446 nucleic acids Human genes 0.000 title abstract 3
- 108091034117 Oligonucleotide Proteins 0.000 abstract 13
- 108091008146 restriction endonucleases Proteins 0.000 abstract 6
- 230000004048 modification Effects 0.000 abstract 5
- 238000012986 modification Methods 0.000 abstract 5
- 230000003100 immobilizing effect Effects 0.000 abstract 2
- 230000000295 complement effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP02023385A EP1411122B1 (de) | 2002-10-18 | 2002-10-18 | Verfahren zur Herstellung von Nukleinsäuremolekülen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE400650T1 true ATE400650T1 (de) | 2008-07-15 |
Family
ID=32039153
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT02023385T ATE400650T1 (de) | 2002-10-18 | 2002-10-18 | Verfahren zur herstellung von nukleinsäuremolekülen |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US7695906B2 (de) |
| EP (1) | EP1411122B1 (de) |
| AT (1) | ATE400650T1 (de) |
| AU (1) | AU2003278103A1 (de) |
| CA (1) | CA2502431C (de) |
| DE (1) | DE60227525D1 (de) |
| DK (1) | DK1411122T3 (de) |
| ES (1) | ES2310197T3 (de) |
| WO (1) | WO2004035781A1 (de) |
Families Citing this family (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9425138D0 (en) | 1994-12-12 | 1995-02-08 | Dynal As | Isolation of nucleic acid |
| US8137906B2 (en) | 1999-06-07 | 2012-03-20 | Sloning Biotechnology Gmbh | Method for the synthesis of DNA fragments |
| ATE414767T1 (de) | 2001-11-22 | 2008-12-15 | Sloning Biotechnology Gmbh | Nukleinsäure-linker und deren verwendung in der gensynthese |
| CA2473376A1 (en) | 2002-01-16 | 2003-07-31 | Invitrogen Dynal As | Method for isolating nucleic acids and protein from a single sample |
| GB0229287D0 (en) * | 2002-12-16 | 2003-01-22 | Dna Res Innovations Ltd | Polyfunctional reagents |
| EP1557464B1 (de) * | 2004-01-23 | 2010-09-29 | Sloning BioTechnology GmbH | Enzymatische Herstellung von Nukleinsäuremolekülen |
| US8053191B2 (en) | 2006-08-31 | 2011-11-08 | Westend Asset Clearinghouse Company, Llc | Iterative nucleic acid assembly using activation of vector-encoded traits |
| US8735064B2 (en) | 2007-12-24 | 2014-05-27 | Bergenbio As | Methods for creating and identifying functional RNA interference elements |
| US9115352B2 (en) | 2008-03-31 | 2015-08-25 | Sloning Biotechnology Gmbh | Method for the preparation of a nucleic acid library |
| WO2010070295A1 (en) | 2008-12-18 | 2010-06-24 | Iti Scotland Limited | Method for assembly of polynucleic acid sequences |
| JP2013504602A (ja) | 2009-09-14 | 2013-02-07 | ダイアックス コーポレーション | 新しく設計したｈｃｃｄｒ３を含む遺伝子パッケージライブラリー |
| US9187777B2 (en) | 2010-05-28 | 2015-11-17 | Gen9, Inc. | Methods and devices for in situ nucleic acid synthesis |
| GB2481425A (en) | 2010-06-23 | 2011-12-28 | Iti Scotland Ltd | Method and device for assembling polynucleic acid sequences |
| CN103249834B (zh) | 2010-08-26 | 2016-10-26 | 纳幕尔杜邦公司 | 生产高水平二十碳五烯酸的重组微生物宿主细胞 |
| US8980589B2 (en) | 2010-08-26 | 2015-03-17 | E I Du Pont De Nemours And Company | Mutant delta-9 elongases and their use in making polyunsaturated fatty acids |
| US9295965B2 (en) * | 2010-11-12 | 2016-03-29 | Gen9, Inc. | Methods and devices for nucleic acid synthesis |
| US10457935B2 (en) | 2010-11-12 | 2019-10-29 | Gen9, Inc. | Protein arrays and methods of using and making the same |
| WO2013017950A1 (en) * | 2011-07-29 | 2013-02-07 | Cellectis | High throughput method for assembly and cloning polynucleotides comprising highly similar polynucleotidic modules |
| ES2737957T3 (es) | 2011-08-26 | 2020-01-17 | Gen9 Inc | Composiciones y métodos para el ensamblaje de alta fidelidad de ácidos nucleicos |
| CN102584955A (zh) * | 2011-12-31 | 2012-07-18 | 北京唯尚立德生物科技有限公司 | 一种转录激活子样效应因子的固相合成方法 |
| DE102012101347B4 (de) * | 2012-02-20 | 2014-01-16 | Markus Fuhrmann | Verfahren zur Herstellung einer Nukleinsäure- Bibliothek mit mindestens zwei benachbarten variablen Codon- Tripletts |
| US9150853B2 (en) | 2012-03-21 | 2015-10-06 | Gen9, Inc. | Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis |
| EP2841601B1 (de) | 2012-04-24 | 2019-03-06 | Gen9, Inc. | Verfahren zum sortieren von nukleinsäuren und zur multiplexierten, vorbereitenden in-vitro-klonung |
| CA2877823A1 (en) | 2012-06-25 | 2014-01-03 | Gen9, Inc. | Methods for nucleic acid assembly and high throughput sequencing |
| GB201612011D0 (en) | 2016-07-11 | 2016-08-24 | Glaxosmithkline Ip Dev Ltd | Novel processes for the production of oligonucleotides |
| GB2566986A (en) | 2017-09-29 | 2019-04-03 | Evonetix Ltd | Error detection during hybridisation of target double-stranded nucleic acid |
| CN108913736B (zh) * | 2018-07-10 | 2021-08-24 | 中国海洋大学 | 单链寡核苷酸的制备方法 |
| US20230075082A1 (en) * | 2021-03-09 | 2023-03-09 | Smobio Technology, Inc. | Method for synthesizing dna |
| WO2022221853A1 (en) | 2021-04-13 | 2022-10-20 | Elegen Corp. | Methods and compositions for cell-free cloning |
| WO2022232134A1 (en) * | 2021-04-26 | 2022-11-03 | Camena Bioscience Limited | Compositions and methods for phosphoramedite-free enzymatic synthesis of nucleic acids |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5273879A (en) * | 1987-07-23 | 1993-12-28 | Syntex (U.S.A.) Inc. | Amplification method for polynucleotide assays |
| DE4343591A1 (de) * | 1993-12-21 | 1995-06-22 | Evotec Biosystems Gmbh | Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes |
| DE19812103A1 (de) | 1998-03-19 | 1999-09-23 | Bernauer Annette | Verfahren zur Synthese von Nucleinsäuremolekülen |
| US6333149B1 (en) | 1999-06-04 | 2001-12-25 | Triad Biotechnology, Inc. | NMR-solve method for rapid identification of bi-ligand drug candidates |
| DE19925862A1 (de) * | 1999-06-07 | 2000-12-14 | Diavir Gmbh | Verfahren zur Synthese von DNA-Fragmenten |
| JP2001127864A (ja) | 1999-10-29 | 2001-05-11 | Suriibii Enterprises Japan:Kk | 携帯電話機用イヤホンマイク及び携帯電話機 |
-
2002
- 2002-10-18 ES ES02023385T patent/ES2310197T3/es not_active Expired - Lifetime
- 2002-10-18 EP EP02023385A patent/EP1411122B1/de not_active Expired - Lifetime
- 2002-10-18 AT AT02023385T patent/ATE400650T1/de active
- 2002-10-18 DE DE60227525T patent/DE60227525D1/de not_active Expired - Lifetime
- 2002-10-18 DK DK02023385T patent/DK1411122T3/da active
-
2003
- 2003-10-17 US US10/531,556 patent/US7695906B2/en not_active Expired - Lifetime
- 2003-10-17 WO PCT/EP2003/011551 patent/WO2004035781A1/en not_active Ceased
- 2003-10-17 CA CA2502431A patent/CA2502431C/en not_active Expired - Lifetime
- 2003-10-17 AU AU2003278103A patent/AU2003278103A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US7695906B2 (en) | 2010-04-13 |
| EP1411122A1 (de) | 2004-04-21 |
| EP1411122B1 (de) | 2008-07-09 |
| CA2502431C (en) | 2013-04-02 |
| DK1411122T3 (da) | 2008-11-03 |
| US20060194202A1 (en) | 2006-08-31 |
| CA2502431A1 (en) | 2004-04-29 |
| AU2003278103A1 (en) | 2004-05-04 |
| DE60227525D1 (de) | 2008-08-21 |
| WO2004035781A1 (en) | 2004-04-29 |
| ES2310197T3 (es) | 2009-01-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE60227525D1 (de) | Verfahren zur Herstellung von Nukleinsäuremolekülen | |
| ATE483020T1 (de) | Enzymatische herstellung von nukleinsäuremolekülen | |
| ATE451342T1 (de) | Verfahren zur herstellung von 1-olefinen durch katalytische spaltung von 1-alkoxyalkanen | |
| ATE483032T1 (de) | Verfahren zur herstellung von oligonukleotiden | |
| ATE464381T1 (de) | Rna-amplifikation auf ligationsbasis | |
| WO2007010251A3 (en) | Preparation of templates for nucleic acid sequencing | |
| ATE414767T1 (de) | Nukleinsäure-linker und deren verwendung in der gensynthese | |
| SI2258847T1 (sl) | Nadaljnje nove oblike molekul interferenčne RNA | |
| ATE551352T1 (de) | Peptidakzeptorbindungsverfahren | |
| DE60002812D1 (de) | Verfahren zur Herstellung atomar scharfer Schneidklingen. | |
| JP2022543778A5 (de) | ||
| DE60213803D1 (en) | Happier mapping | |
| WO2007017229A3 (de) | Verfahren für die kontinuierliche zielgerichtete evolution von proteinen in vitro | |
| WO2005021786A8 (en) | A method of sequencing nucleic acids by ligation of labelled oligonucleotides | |
| ATE431407T1 (de) | Verfahren zur herstellung von dna | |
| WO2003102232A3 (de) | Vermehrung von ribonukleinsäuren | |
| WO2005054270A3 (en) | Identification and design of sirna molecules | |
| WO2002083943A3 (de) | Mikroarray-verfahren zur anreicherung von dna-fragmenten aus komplexen mischungen | |
| ATE360065T1 (de) | Verfahren zur herstellung von matrizen-dna und verfahren zur proteinherstellung in einem zellfreien proteinsynthesesystem unter verwendung davon | |
| ATE358172T1 (de) | Verfahren zur herstellung von olefinen. | |
| ATE538200T2 (de) | Verfahren zur herstellung einer nukleinsäurenbibliothek | |
| ATE361312T1 (de) | Verfahren zur herstellung von glycopeptid- phosphonat-derivaten | |
| DE60123334D1 (de) | Methode für die Produktion von Nukleotiden durch Fermentierung | |
| DE60117525D1 (de) | Verfahren zur herstellung von laktonen | |
| ATE223499T1 (de) | Von kreisförmiger matrize abhängige kettenreaktion |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |
Ref document number: 1411122 Country of ref document: EP |