ATE401422T1 - Verfahren zur erzeugung einer bibliothek aus bakterienklonen mit unterschiedlichen genexpressionsniveaus - Google Patents

Verfahren zur erzeugung einer bibliothek aus bakterienklonen mit unterschiedlichen genexpressionsniveaus

Info

Publication number
ATE401422T1
ATE401422T1 AT03724100T AT03724100T ATE401422T1 AT E401422 T1 ATE401422 T1 AT E401422T1 AT 03724100 T AT03724100 T AT 03724100T AT 03724100 T AT03724100 T AT 03724100T AT E401422 T1 ATE401422 T1 AT E401422T1
Authority
AT
Austria
Prior art keywords
library
expression levels
generating
gene expression
bacterial clones
Prior art date
Application number
AT03724100T
Other languages
English (en)
Inventor
Philippe Soucaille
Marguerite A Cervin
Fernando Valle
Original Assignee
Genencor Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Int filed Critical Genencor Int
Application granted granted Critical
Publication of ATE401422T1 publication Critical patent/ATE401422T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT03724100T 2002-04-22 2003-04-18 Verfahren zur erzeugung einer bibliothek aus bakterienklonen mit unterschiedlichen genexpressionsniveaus ATE401422T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US37462702P 2002-04-22 2002-04-22

Publications (1)

Publication Number Publication Date
ATE401422T1 true ATE401422T1 (de) 2008-08-15

Family

ID=29251213

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03724100T ATE401422T1 (de) 2002-04-22 2003-04-18 Verfahren zur erzeugung einer bibliothek aus bakterienklonen mit unterschiedlichen genexpressionsniveaus

Country Status (9)

Country Link
US (1) US20060014146A1 (de)
EP (1) EP1501947B1 (de)
JP (1) JP2005523015A (de)
AT (1) ATE401422T1 (de)
AU (1) AU2003230982A1 (de)
CA (1) CA2483338C (de)
DE (1) DE60322210D1 (de)
DK (1) DK1501947T3 (de)
WO (1) WO2003089605A2 (de)

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EP2227553A4 (de) * 2007-11-30 2011-01-19 Scarab Genomics Llc Lac-expressionssystem
US20110111977A1 (en) * 2008-07-03 2011-05-12 Pfenex, Inc. High throughput screening method and use thereof to identify a production platform for a multifunctional binding protein
WO2011053957A2 (en) * 2009-11-02 2011-05-05 Gen9, Inc. Compositions and methods for the regulation of multiple genes of interest in a cell
EP3000883B8 (de) 2010-11-12 2018-02-28 Gen9, Inc. Verfahren und vorrichtungen für nukleinsäuresynthese
US10457935B2 (en) 2010-11-12 2019-10-29 Gen9, Inc. Protein arrays and methods of using and making the same
IL280334B2 (en) 2011-08-26 2023-09-01 Gen9 Inc Preparations and methods for high-fidelity assembly of nucleic acids
EP2573172A1 (de) 2011-09-21 2013-03-27 Heinrich-Heine-Universität Düsseldorf Mittel und Verfahren zur Rhamnolipidherstellung
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
CN104603286B (zh) 2012-04-24 2020-07-31 Gen9股份有限公司 在体外克隆中分选核酸和多重制备物的方法
WO2014004393A1 (en) 2012-06-25 2014-01-03 Gen9, Inc. Methods for nucleic acid assembly and high throughput sequencing
TWI805996B (zh) 2013-08-05 2023-06-21 美商扭轉生物科技有限公司 重新合成之基因庫
WO2016126987A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Compositions and methods for synthetic gene assembly
WO2016126882A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
US10907180B2 (en) 2015-07-08 2021-02-02 Rheinisch-Westfaelische Technische Hochshule Aachen (RWTH) Extracellular production of designer hydroxyalkanoyloxy alkanoic acids with recombinant bacteria
HK1258869A1 (zh) 2015-09-18 2019-11-22 Twist Bioscience Corporation 寡核酸变体文库及其合成
CN108698012A (zh) 2015-09-22 2018-10-23 特韦斯特生物科学公司 用于核酸合成的柔性基底
CN108603307A (zh) 2015-12-01 2018-09-28 特韦斯特生物科学公司 功能化表面及其制备
WO2018038772A1 (en) 2016-08-22 2018-03-01 Twist Bioscience Corporation De novo synthesized nucleic acid libraries
EP3516528B1 (de) 2016-09-21 2025-10-15 Atlas Data Storage, Inc. Nukleinsäurebasierte datenspeicherung
WO2018112426A1 (en) 2016-12-16 2018-06-21 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
KR20240158370A (ko) 2017-02-22 2024-11-04 트위스트 바이오사이언스 코포레이션 핵산 기반 데이터 저장
CA3056388A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
KR102628876B1 (ko) 2017-06-12 2024-01-23 트위스트 바이오사이언스 코포레이션 심리스 핵산 어셈블리를 위한 방법
CN111566125A (zh) 2017-09-11 2020-08-21 特韦斯特生物科学公司 Gpcr结合蛋白及其合成
US10894242B2 (en) 2017-10-20 2021-01-19 Twist Bioscience Corporation Heated nanowells for polynucleotide synthesis
US11608504B2 (en) 2017-12-21 2023-03-21 Glycom A/S Nucleic acid construct for in vitro and in vivo gene expression
EP3735459B1 (de) 2018-01-04 2026-03-04 Atlas Data Storage, Inc. Digitale informationsspeicherung auf dna-basis
CN110317807A (zh) * 2018-03-29 2019-10-11 中国科学院天津工业生物技术研究所 一种谷氨酸棒杆菌人工启动子文库
SG11202011467RA (en) 2018-05-18 2020-12-30 Twist Bioscience Corp Polynucleotides, reagents, and methods for nucleic acid hybridization
CN113692409B (zh) 2018-12-26 2025-01-10 特韦斯特生物科学公司 高度准确的从头多核苷酸合成
EP3930753A4 (de) 2019-02-26 2023-03-29 Twist Bioscience Corporation Variante nukleinsäurebibliotheken für glp1-rezeptor
US11492728B2 (en) 2019-02-26 2022-11-08 Twist Bioscience Corporation Variant nucleic acid libraries for antibody optimization
CA3144644A1 (en) 2019-06-21 2020-12-24 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
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US12570750B2 (en) 2019-12-09 2026-03-10 Twist Bioscience Corporation Antibodies that bind adenosine A2A receptors and methods of use thereof to treat cancer and neurological diseases
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US5851808A (en) * 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination

Also Published As

Publication number Publication date
AU2003230982A8 (en) 2003-11-03
WO2003089605A2 (en) 2003-10-30
DE60322210D1 (de) 2008-08-28
EP1501947B1 (de) 2008-07-16
CA2483338A1 (en) 2003-10-30
WO2003089605A3 (en) 2004-12-02
DK1501947T3 (da) 2008-11-17
JP2005523015A (ja) 2005-08-04
AU2003230982A1 (en) 2003-11-03
US20060014146A1 (en) 2006-01-19
EP1501947A4 (de) 2006-04-26
EP1501947A2 (de) 2005-02-02
CA2483338C (en) 2014-10-14

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