ATE401422T1 - Verfahren zur erzeugung einer bibliothek aus bakterienklonen mit unterschiedlichen genexpressionsniveaus - Google Patents

Verfahren zur erzeugung einer bibliothek aus bakterienklonen mit unterschiedlichen genexpressionsniveaus

Info

Publication number
ATE401422T1
ATE401422T1 AT03724100T AT03724100T ATE401422T1 AT E401422 T1 ATE401422 T1 AT E401422T1 AT 03724100 T AT03724100 T AT 03724100T AT 03724100 T AT03724100 T AT 03724100T AT E401422 T1 ATE401422 T1 AT E401422T1
Authority
AT
Austria
Prior art keywords
library
expression levels
generating
gene expression
bacterial clones
Prior art date
Application number
AT03724100T
Other languages
English (en)
Inventor
Philippe Soucaille
Marguerite A Cervin
Fernando Valle
Original Assignee
Genencor Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Int filed Critical Genencor Int
Application granted granted Critical
Publication of ATE401422T1 publication Critical patent/ATE401422T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT03724100T 2002-04-22 2003-04-18 Verfahren zur erzeugung einer bibliothek aus bakterienklonen mit unterschiedlichen genexpressionsniveaus ATE401422T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US37462702P 2002-04-22 2002-04-22

Publications (1)

Publication Number Publication Date
ATE401422T1 true ATE401422T1 (de) 2008-08-15

Family

ID=29251213

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03724100T ATE401422T1 (de) 2002-04-22 2003-04-18 Verfahren zur erzeugung einer bibliothek aus bakterienklonen mit unterschiedlichen genexpressionsniveaus

Country Status (9)

Country Link
US (1) US20060014146A1 (de)
EP (1) EP1501947B1 (de)
JP (1) JP2005523015A (de)
AT (1) ATE401422T1 (de)
AU (1) AU2003230982A1 (de)
CA (1) CA2483338C (de)
DE (1) DE60322210D1 (de)
DK (1) DK1501947T3 (de)
WO (1) WO2003089605A2 (de)

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RU2396336C2 (ru) 2007-09-27 2010-08-10 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) СПОСОБ ПОЛУЧЕНИЯ АМИНОКИСЛОТ С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ, ПРИНАДЛЕЖАЩЕЙ К РОДУ Escherichia
EP2227553A4 (de) * 2007-11-30 2011-01-19 Scarab Genomics Llc Lac-expressionssystem
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WO2011053957A2 (en) * 2009-11-02 2011-05-05 Gen9, Inc. Compositions and methods for the regulation of multiple genes of interest in a cell
CA2817697C (en) 2010-11-12 2021-11-16 Gen9, Inc. Methods and devices for nucleic acids synthesis
WO2012064975A1 (en) 2010-11-12 2012-05-18 Gen9, Inc. Protein arrays and methods of using and making the same
JP2014526899A (ja) 2011-08-26 2014-10-09 ジェン９・インコーポレイテッド 核酸の高忠実度アセンブリのための組成物および方法
EP2573172A1 (de) 2011-09-21 2013-03-27 Heinrich-Heine-Universität Düsseldorf Mittel und Verfahren zur Rhamnolipidherstellung
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
CA2871505C (en) 2012-04-24 2021-10-12 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
WO2014004393A1 (en) 2012-06-25 2014-01-03 Gen9, Inc. Methods for nucleic acid assembly and high throughput sequencing
KR102160389B1 (ko) 2013-08-05 2020-09-28 트위스트 바이오사이언스 코포레이션 드 노보 합성된 유전자 라이브러리
CA2975855C (en) 2015-02-04 2025-09-23 Twist Bioscience Corporation SYNTHETIC GENE COMPOSITIONS AND ASSEMBLY METHODS
CA2975852A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
WO2016172377A1 (en) 2015-04-21 2016-10-27 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
US10907180B2 (en) 2015-07-08 2021-02-02 Rheinisch-Westfaelische Technische Hochshule Aachen (RWTH) Extracellular production of designer hydroxyalkanoyloxy alkanoic acids with recombinant bacteria
KR20180050411A (ko) 2015-09-18 2018-05-14 트위스트 바이오사이언스 코포레이션 올리고핵산 변이체 라이브러리 및 그의 합성
WO2017053450A1 (en) 2015-09-22 2017-03-30 Twist Bioscience Corporation Flexible substrates for nucleic acid synthesis
EP3384077A4 (de) 2015-12-01 2019-05-08 Twist Bioscience Corporation Funktionalisierte oberflächen und herstellung davon
WO2018038772A1 (en) 2016-08-22 2018-03-01 Twist Bioscience Corporation De novo synthesized nucleic acid libraries
EP4664304A3 (de) 2016-09-21 2026-01-21 Atlas Data Storage, Inc. Nukleinsäurebasierte datenspeicherung
GB2573069A (en) 2016-12-16 2019-10-23 Twist Bioscience Corp Variant libraries of the immunological synapse and synthesis thereof
WO2018156792A1 (en) 2017-02-22 2018-08-30 Twist Bioscience Corporation Nucleic acid based data storage
CA3056388A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
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WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
CN111566125A (zh) 2017-09-11 2020-08-21 特韦斯特生物科学公司 Gpcr结合蛋白及其合成
CN111565834B (zh) 2017-10-20 2022-08-26 特韦斯特生物科学公司 用于多核苷酸合成的加热的纳米孔
EP3728596A4 (de) 2017-12-21 2021-08-25 Glycom A/S Nukleinsäurekonstrukt für in-vitro- und in-vivo-genexpression
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CN110317807A (zh) * 2018-03-29 2019-10-11 中国科学院天津工业生物技术研究所 一种谷氨酸棒杆菌人工启动子文库
EP3814497A4 (de) 2018-05-18 2022-03-02 Twist Bioscience Corporation Polynukleotide, reagenzien und verfahren zur nukleinsäurehybridisierung
EP3902816A4 (de) 2018-12-26 2022-09-14 Twist Bioscience Corporation Hochgenaue de-novo-polynukleotidsynthese
CA3131689A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for glp1 receptor
KR20210144698A (ko) 2019-02-26 2021-11-30 트위스트 바이오사이언스 코포레이션 항체 최적화를 위한 변이 핵산 라이브러리
EP3987019A4 (de) 2019-06-21 2023-04-19 Twist Bioscience Corporation Barcode-basierte nukleinsäuresequenzanordnung
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DE69739121D1 (de) * 1996-08-23 2009-01-02 Peter Ruhdal Jensen Künstliche promotorbibliotheken für ausgewählte organismen sowie daraus entstammende promotoren
US5851808A (en) * 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination

Also Published As

Publication number Publication date
CA2483338A1 (en) 2003-10-30
CA2483338C (en) 2014-10-14
AU2003230982A1 (en) 2003-11-03
JP2005523015A (ja) 2005-08-04
EP1501947B1 (de) 2008-07-16
AU2003230982A8 (en) 2003-11-03
DK1501947T3 (da) 2008-11-17
EP1501947A2 (de) 2005-02-02
WO2003089605A3 (en) 2004-12-02
EP1501947A4 (de) 2006-04-26
DE60322210D1 (de) 2008-08-28
WO2003089605A2 (en) 2003-10-30
US20060014146A1 (en) 2006-01-19

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