ATE514775T1 - Endenmodifikation zur verhinderung einer überrepräsentierung von fragmenten - Google Patents
Endenmodifikation zur verhinderung einer überrepräsentierung von fragmentenInfo
- Publication number
- ATE514775T1 ATE514775T1 AT07705158T AT07705158T ATE514775T1 AT E514775 T1 ATE514775 T1 AT E514775T1 AT 07705158 T AT07705158 T AT 07705158T AT 07705158 T AT07705158 T AT 07705158T AT E514775 T1 ATE514775 T1 AT E514775T1
- Authority
- AT
- Austria
- Prior art keywords
- representation
- fragments
- prevent over
- modified library
- end modification
- Prior art date
Links
- 239000012634 fragment Substances 0.000 title 1
- 230000004048 modification Effects 0.000 title 1
- 238000012986 modification Methods 0.000 title 1
- 238000000034 method Methods 0.000 abstract 3
- 102000040430 polynucleotide Human genes 0.000 abstract 3
- 108091033319 polynucleotide Proteins 0.000 abstract 3
- 239000002157 polynucleotide Substances 0.000 abstract 3
- 230000003321 amplification Effects 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 239000007790 solid phase Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US77135806P | 2006-02-08 | 2006-02-08 | |
| PCT/GB2007/000427 WO2007091064A1 (en) | 2006-02-08 | 2007-02-07 | End modification to prevent over-representation of fragments |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE514775T1 true ATE514775T1 (de) | 2011-07-15 |
Family
ID=37963897
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT07705158T ATE514775T1 (de) | 2006-02-08 | 2007-02-07 | Endenmodifikation zur verhinderung einer überrepräsentierung von fragmenten |
Country Status (4)
| Country | Link |
|---|---|
| US (2) | US9012184B2 (de) |
| EP (1) | EP1991675B1 (de) |
| AT (1) | ATE514775T1 (de) |
| WO (1) | WO2007091064A1 (de) |
Families Citing this family (51)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11111544B2 (en) | 2005-07-29 | 2021-09-07 | Natera, Inc. | System and method for cleaning noisy genetic data and determining chromosome copy number |
| US10083273B2 (en) | 2005-07-29 | 2018-09-25 | Natera, Inc. | System and method for cleaning noisy genetic data and determining chromosome copy number |
| US10081839B2 (en) | 2005-07-29 | 2018-09-25 | Natera, Inc | System and method for cleaning noisy genetic data and determining chromosome copy number |
| US9424392B2 (en) | 2005-11-26 | 2016-08-23 | Natera, Inc. | System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals |
| US11111543B2 (en) | 2005-07-29 | 2021-09-07 | Natera, Inc. | System and method for cleaning noisy genetic data and determining chromosome copy number |
| GB0522310D0 (en) * | 2005-11-01 | 2005-12-07 | Solexa Ltd | Methods of preparing libraries of template polynucleotides |
| US9012184B2 (en) * | 2006-02-08 | 2015-04-21 | Illumina Cambridge Limited | End modification to prevent over-representation of fragments |
| EP2049682A2 (de) * | 2006-07-31 | 2009-04-22 | Illumina Cambridge Limited | Verfahren zur herstellung von bibliotheken unter vermeidung der bildung von adaptordimeren |
| WO2008093098A2 (en) | 2007-02-02 | 2008-08-07 | Illumina Cambridge Limited | Methods for indexing samples and sequencing multiple nucleotide templates |
| US20110178719A1 (en) | 2008-08-04 | 2011-07-21 | Gene Security Network, Inc. | Methods for Allele Calling and Ploidy Calling |
| EP2473638B1 (de) | 2009-09-30 | 2017-08-09 | Natera, Inc. | Verfahren zur nichtinvasiven pränatalen ploidiezuordnung |
| US11326208B2 (en) | 2010-05-18 | 2022-05-10 | Natera, Inc. | Methods for nested PCR amplification of cell-free DNA |
| US11408031B2 (en) | 2010-05-18 | 2022-08-09 | Natera, Inc. | Methods for non-invasive prenatal paternity testing |
| US11339429B2 (en) | 2010-05-18 | 2022-05-24 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US11322224B2 (en) | 2010-05-18 | 2022-05-03 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US12221653B2 (en) | 2010-05-18 | 2025-02-11 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US10316362B2 (en) | 2010-05-18 | 2019-06-11 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US11939634B2 (en) | 2010-05-18 | 2024-03-26 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US12152275B2 (en) | 2010-05-18 | 2024-11-26 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US11332793B2 (en) | 2010-05-18 | 2022-05-17 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US12545960B2 (en) | 2010-05-18 | 2026-02-10 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US11332785B2 (en) | 2010-05-18 | 2022-05-17 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US8825412B2 (en) | 2010-05-18 | 2014-09-02 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US20190010543A1 (en) | 2010-05-18 | 2019-01-10 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US9677118B2 (en) | 2014-04-21 | 2017-06-13 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| EP2656263B1 (de) | 2010-12-22 | 2019-11-06 | Natera, Inc. | Verfahren für nichtinvasiven pränatalen vaterschaftstest |
| JP6153874B2 (ja) | 2011-02-09 | 2017-06-28 | ナテラ， インコーポレイテッド | 非侵襲的出生前倍数性呼び出しのための方法 |
| CN102876658A (zh) * | 2011-07-12 | 2013-01-16 | 北京唯尚立德生物科技有限公司 | 一种大规模合成长链核酸分子的方法 |
| CN107055468A (zh) * | 2012-06-01 | 2017-08-18 | 欧洲分子生物学实验室 | Dna中数字信息的高容量存储 |
| US20140051585A1 (en) * | 2012-08-15 | 2014-02-20 | Natera, Inc. | Methods and compositions for reducing genetic library contamination |
| US20140100126A1 (en) | 2012-08-17 | 2014-04-10 | Natera, Inc. | Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data |
| US9499870B2 (en) | 2013-09-27 | 2016-11-22 | Natera, Inc. | Cell free DNA diagnostic testing standards |
| US10262755B2 (en) | 2014-04-21 | 2019-04-16 | Natera, Inc. | Detecting cancer mutations and aneuploidy in chromosomal segments |
| US10577655B2 (en) | 2013-09-27 | 2020-03-03 | Natera, Inc. | Cell free DNA diagnostic testing standards |
| WO2015113463A1 (zh) | 2014-01-28 | 2015-08-06 | 广东欧珀移动通信有限公司 | 电源适配器、终端和处理充电回路阻抗异常的方法 |
| US12492429B2 (en) | 2014-04-21 | 2025-12-09 | Natera, Inc. | Detecting mutations and ploidy in chromosomal segments |
| CN113774132A (zh) | 2014-04-21 | 2021-12-10 | 纳特拉公司 | 检测染色体片段中的突变和倍性 |
| US20180173846A1 (en) | 2014-06-05 | 2018-06-21 | Natera, Inc. | Systems and Methods for Detection of Aneuploidy |
| DK3294906T3 (en) | 2015-05-11 | 2024-08-05 | Natera Inc | Methods for determining ploidy |
| US11248262B2 (en) * | 2015-08-24 | 2022-02-15 | Qiagen Gmbh | Method for generating a RNA-sequencing library |
| DK3443119T3 (da) | 2016-04-15 | 2022-05-23 | Natera Inc | Fremgangsmåde til bestemmelse af lunge cancer |
| WO2018067517A1 (en) | 2016-10-04 | 2018-04-12 | Natera, Inc. | Methods for characterizing copy number variation using proximity-litigation sequencing |
| GB201618485D0 (en) | 2016-11-02 | 2016-12-14 | Ucl Business Plc | Method of detecting tumour recurrence |
| US10011870B2 (en) | 2016-12-07 | 2018-07-03 | Natera, Inc. | Compositions and methods for identifying nucleic acid molecules |
| WO2018156418A1 (en) | 2017-02-21 | 2018-08-30 | Natera, Inc. | Compositions, methods, and kits for isolating nucleic acids |
| WO2019118926A1 (en) | 2017-12-14 | 2019-06-20 | Tai Diagnostics, Inc. | Assessing graft suitability for transplantation |
| WO2019161244A1 (en) | 2018-02-15 | 2019-08-22 | Natera, Inc. | Methods for isolating nucleic acids with size selection |
| DK3781714T3 (da) | 2018-04-14 | 2026-03-30 | Natera Inc | Fremgangsmåder til cancerpåvisning og -overvågning ved personlig påvisning af cirkulerende tumor-dna |
| US12234509B2 (en) | 2018-07-03 | 2025-02-25 | Natera, Inc. | Methods for detection of donor-derived cell-free DNA |
| EP3980559A1 (de) | 2019-06-06 | 2022-04-13 | Natera, Inc. | Verfahren zur detektion von immunzellen-dna und zur überwachung des immunsystems |
| CN117597453A (zh) | 2021-06-23 | 2024-02-23 | 伊鲁米纳公司 | 用于测序试剂的组合物、方法、试剂盒、料筒和系统 |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5817797A (en) * | 1988-06-01 | 1998-10-06 | The United States Of America As Represented By The Department Of Health And Human Services | Sequencing DNA; a modification of the polymerase chain reaction |
| DE19518505A1 (de) * | 1995-05-19 | 1996-11-21 | Max Planck Gesellschaft | Verfahren zur Genexpressionsanalyse |
| DE19849348A1 (de) * | 1998-10-26 | 2000-04-27 | Univ Ludwigs Albert | Linear Amplification mediated PCR (=LAM PCR) |
| US20030049657A1 (en) * | 1998-11-13 | 2003-03-13 | Cherry Joshua L. | Use of primers containing non-replicatable residues for improved cycle-sequencing of nucleic acids |
| AU782676B2 (en) * | 1999-09-30 | 2005-08-18 | Corixa Corporation | Immunologically significant herpes simplex virus antigens |
| EP1451365A4 (de) * | 2001-11-13 | 2006-09-13 | Rubicon Genomics Inc | Dna-amplifikation und -sequenzierung mit durch zufallsfragmentierung erzeugten dna-molekülen |
| CA2513889A1 (en) * | 2003-01-29 | 2004-08-19 | 454 Corporation | Double ended sequencing |
| EP1606417A2 (de) * | 2003-03-07 | 2005-12-21 | Rubicon Genomics Inc. | In-vitro-dna-immortalisierung und gesamtgenomamplifikation unter verwendung von aus willkürlich fragmentierter dna erzeugten bibliotheken |
| EP1682680B2 (de) * | 2003-10-31 | 2018-03-21 | AB Advanced Genetic Analysis Corporation | Verfahren zur herstellung eines gepaarten tag aus einer nukleinsäuresequenz sowie verfahren zur verwendung davon |
| US7867703B2 (en) * | 2004-08-26 | 2011-01-11 | Agilent Technologies, Inc. | Element defined sequence complexity reduction |
| US20090233291A1 (en) * | 2005-06-06 | 2009-09-17 | 454 Life Sciences Corporation | Paired end sequencing |
| GB0522310D0 (en) * | 2005-11-01 | 2005-12-07 | Solexa Ltd | Methods of preparing libraries of template polynucleotides |
| US20070172839A1 (en) * | 2006-01-24 | 2007-07-26 | Smith Douglas R | Asymmetrical adapters and methods of use thereof |
| US9012184B2 (en) * | 2006-02-08 | 2015-04-21 | Illumina Cambridge Limited | End modification to prevent over-representation of fragments |
| US20080194414A1 (en) * | 2006-04-24 | 2008-08-14 | Albert Thomas J | Enrichment and sequence analysis of genomic regions |
-
2007
- 2007-02-07 US US12/223,761 patent/US9012184B2/en active Active
- 2007-02-07 EP EP07705158A patent/EP1991675B1/de active Active
- 2007-02-07 WO PCT/GB2007/000427 patent/WO2007091064A1/en not_active Ceased
- 2007-02-07 AT AT07705158T patent/ATE514775T1/de not_active IP Right Cessation
-
2015
- 2015-03-24 US US14/666,839 patent/US10006081B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US20090176662A1 (en) | 2009-07-09 |
| WO2007091064A8 (en) | 2011-02-17 |
| US20150197789A1 (en) | 2015-07-16 |
| EP1991675B1 (de) | 2011-06-29 |
| EP1991675A1 (de) | 2008-11-19 |
| WO2007091064A1 (en) | 2007-08-16 |
| US10006081B2 (en) | 2018-06-26 |
| US9012184B2 (en) | 2015-04-21 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |