ATE405677T1 - Verfahren zur kontrollierten freisetzung enzymatischer reaktionskomponenten - Google Patents

Verfahren zur kontrollierten freisetzung enzymatischer reaktionskomponenten

Info

Publication number
ATE405677T1
ATE405677T1 AT04758773T AT04758773T ATE405677T1 AT E405677 T1 ATE405677 T1 AT E405677T1 AT 04758773 T AT04758773 T AT 04758773T AT 04758773 T AT04758773 T AT 04758773T AT E405677 T1 ATE405677 T1 AT E405677T1
Authority
AT
Austria
Prior art keywords
controlled release
enzymatic reaction
reaction components
compositions
water
Prior art date
Application number
AT04758773T
Other languages
English (en)
Inventor
Konstantin Ignatov
Vladimir Kramarov
Dimitrij Plachov
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Promega Corp filed Critical Promega Corp
Application granted granted Critical
Publication of ATE405677T1 publication Critical patent/ATE405677T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT04758773T 2003-04-04 2004-04-01 Verfahren zur kontrollierten freisetzung enzymatischer reaktionskomponenten ATE405677T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE10315640A DE10315640A1 (de) 2003-04-04 2003-04-04 Verfahren zur kontrollierten Freisetzung von Komponenten in eine Lösung

Publications (1)

Publication Number Publication Date
ATE405677T1 true ATE405677T1 (de) 2008-09-15

Family

ID=32981089

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04758773T ATE405677T1 (de) 2003-04-04 2004-04-01 Verfahren zur kontrollierten freisetzung enzymatischer reaktionskomponenten

Country Status (9)

Country Link
US (2) US7306929B2 (de)
EP (1) EP1611257B1 (de)
JP (1) JP2006521827A (de)
AT (1) ATE405677T1 (de)
AU (1) AU2004227368B2 (de)
CA (1) CA2521476A1 (de)
DE (2) DE10315640A1 (de)
ES (1) ES2310745T3 (de)
WO (1) WO2004090169A2 (de)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60322044D1 (de) 2002-02-21 2008-08-21 Asm Scient Inc Rekombinase-polymerase-amplifikation
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
US7399590B2 (en) 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
WO2006119419A2 (en) * 2005-05-03 2006-11-09 Geunsook Jeon Materials and kits for use in hot-start pcr, and methods of amplifying nucleic acids in a polymerase chain reaction
JP5027126B2 (ja) 2005-07-25 2012-09-19 アリーア サン ディエゴ， インコーポレイテッド リコンビナーゼポリメラーゼ増幅を多重化するための方法
JP2009535053A (ja) 2006-05-04 2009-10-01 エーエスエム サイエンティフィック， インコーポレイテッド レコンビナーゼポリメラーゼ増幅
WO2011120020A1 (en) 2010-03-25 2011-09-29 Quantalife, Inc. Droplet transport system for detection
CN102405402A (zh) 2008-09-23 2012-04-04 阔达生命有限公司 基于液滴的测定系统
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US12090480B2 (en) 2008-09-23 2024-09-17 Bio-Rad Laboratories, Inc. Partition-based method of analysis
US9194861B2 (en) 2009-09-02 2015-11-24 Bio-Rad Laboratories, Inc. Method of mixing fluids by coalescence of multiple emulsions
US10512910B2 (en) 2008-09-23 2019-12-24 Bio-Rad Laboratories, Inc. Droplet-based analysis method
US9492797B2 (en) 2008-09-23 2016-11-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US9598725B2 (en) 2010-03-02 2017-03-21 Bio-Rad Laboratories, Inc. Emulsion chemistry for encapsulated droplets
US12162008B2 (en) 2008-09-23 2024-12-10 Bio-Rad Laboratories, Inc. Partition-based method of analysis
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US8633015B2 (en) 2008-09-23 2014-01-21 Bio-Rad Laboratories, Inc. Flow-based thermocycling system with thermoelectric cooler
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
US9399215B2 (en) 2012-04-13 2016-07-26 Bio-Rad Laboratories, Inc. Sample holder with a well having a wicking promoter
US9764322B2 (en) 2008-09-23 2017-09-19 Bio-Rad Laboratories, Inc. System for generating droplets with pressure monitoring
US11130128B2 (en) 2008-09-23 2021-09-28 Bio-Rad Laboratories, Inc. Detection method for a target nucleic acid
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
JP5847076B2 (ja) 2009-05-20 2016-01-20 バイオサイト インコーポレイテッド Ｄｎａグリコシラーゼ／リアーゼおよびａｐエンドヌクレアーゼ基質
EP3360974B1 (de) * 2009-06-05 2025-04-09 Abbott Diagnostics Scarborough, Inc. Reagentien zur rekombinase polymerase amplifikation
CA2767113A1 (en) 2010-03-25 2011-09-29 Bio-Rad Laboratories, Inc. Detection system for droplet-based assays
EP2550528B1 (de) 2010-03-25 2019-09-11 Bio-Rad Laboratories, Inc. Tropfenerzeugung für tropfenbasierte prüfungen
CA2816712C (en) 2010-11-01 2018-12-11 Donald A. Masquelier System for forming emulsions
US12097495B2 (en) 2011-02-18 2024-09-24 Bio-Rad Laboratories, Inc. Methods and compositions for detecting genetic material
JP2014509865A (ja) 2011-03-18 2014-04-24 バイオ−ラッド・ラボラトリーズ・インコーポレーテッド シグナルの組合せ使用による多重化デジタルアッセイ
EP2702175B1 (de) 2011-04-25 2018-08-08 Bio-Rad Laboratories, Inc. Verfahren und zusammensetzungen zur nukleinsäureanalyse
WO2013019751A1 (en) 2011-07-29 2013-02-07 Bio-Rad Laboratories, Inc., Library characterization by digital assay
JPWO2014103980A1 (ja) * 2012-12-28 2017-01-12 和光純薬工業株式会社 Ｄｎａ中のヒドロキシメチル化シトシンの検出方法及び検出用試薬キット

Family Cites Families (113)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US419909A (en) * 1890-01-21 Post-hole auger
US3761437A (en) * 1969-09-26 1973-09-25 American Optical Corp Laser energy absorbing composition containing a copper salt
DE2029419C3 (de) * 1969-09-26 1974-02-07 American Optical Corp., Southbridge, Mass. (V.St.A.) Durch Laserstrahlen löschbare Druckfarbe
US4098728A (en) * 1976-01-02 1978-07-04 Solomon Rosenblatt Medical surgical sponge and method of making same
US4247498A (en) * 1976-08-30 1981-01-27 Akzona Incorporated Methods for making microporous products
US4519909A (en) 1977-07-11 1985-05-28 Akzona Incorporated Microporous products
US4186189A (en) * 1977-09-28 1980-01-29 Ethicon, Inc. Absorbable pharmaceutical compositions based on poly(alkylene oxalates)
US4384975A (en) * 1980-06-13 1983-05-24 Sandoz, Inc. Process for preparation of microspheres
US4507414A (en) * 1982-05-14 1985-03-26 The B. F. Goodrich Company Smoke retardant vinyl halide polymer compositions
DK151608C (da) * 1982-08-13 1988-06-20 Benzon As Alfred Fremgangsmaade til fremstilling af et farmaceutisk peroralt polydepotpraeparat med kontrolleret afgivelse
HU187215B (en) * 1983-01-26 1985-11-28 Egyt Gyogyszervegyeszeti Gyar Method for producing pharmaceutical product of high actor content and prolonged effect
US4469743A (en) * 1983-03-14 1984-09-04 E. I. Du Pont De Nemours And Company Polyvinyl butyral laminates
US5135477A (en) * 1984-10-29 1992-08-04 Medtronic, Inc. Iontophoretic drug delivery
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4789630A (en) 1985-10-04 1988-12-06 Cetus Corporation Ionic compounds containing the cationic meriquinone of a benzidine
US5455170A (en) * 1986-08-22 1995-10-03 Hoffmann-La Roche Inc. Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05
US5466591A (en) * 1986-08-22 1995-11-14 Hoffmann-La Roche Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US5374553A (en) 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US5079352A (en) * 1986-08-22 1992-01-07 Cetus Corporation Purified thermostable enzyme
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5283189A (en) * 1987-02-09 1994-02-01 Research Development Corporation Of Japan β-galactosidase from Thermus sp
US4830855A (en) * 1987-11-13 1989-05-16 Landec Labs, Inc. Temperature-controlled active agent dispenser
JP2531246B2 (ja) * 1988-08-26 1996-09-04 東洋紡績株式会社 耐熱性ｄｎａポリメラ―ゼおよびその製法
US5215907A (en) * 1989-02-24 1993-06-01 Oklahoma Medical Research Foundation Thermostable acid protease from sulfolobus acidocaldarius
GB8905269D0 (en) * 1989-03-08 1989-04-19 Univ Bristol Novel modified nad-dependent dehydrogenases
HU203041B (en) * 1989-03-14 1991-05-28 Egyt Gyogyszervegyeszeti Gyar Process for producing pharmaceutical compositions of controlled releasing factor containing nifedipin
US5071648A (en) 1989-04-06 1991-12-10 Merocel Corporation Polymeric broad-spectrum antimicrobial materials
DE69131891T2 (de) * 1990-02-16 2000-06-15 F. Hoffmann-La Roche Ag, Basel Verbesserungen in der spezifität und zweckmässigkeit der polymerase-kettenreaktion
JP3078859B2 (ja) * 1990-02-23 2000-08-21 武田薬品工業株式会社 安定な放出制御性製剤用コーティング剤
US5192675A (en) * 1990-03-20 1993-03-09 Life Technologies, Inc. Cloned KpnI restriction-modification system
US5091187A (en) * 1990-04-26 1992-02-25 Haynes Duncan H Phospholipid-coated microcrystals: injectable formulations of water-insoluble drugs
US5500363A (en) * 1990-04-26 1996-03-19 New England Biolabs, Inc. Recombinant thermostable DNA polymerase from archaebacteria
US5246707A (en) * 1990-04-26 1993-09-21 Haynes Duncan H Sustained release delivery of water-soluble bio-molecules and drugs using phospholipid-coated microcrystals, microdroplets and high-concentration liposomes
US5494810A (en) * 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US6787338B2 (en) * 1990-06-04 2004-09-07 The University Of Utah Method for rapid thermal cycling of biological samples
US5643777A (en) * 1990-06-15 1997-07-01 Novo Nordisk A/S Thermostable protease from thermococcus
DK146090D0 (da) * 1990-06-15 1990-06-15 Novo Nordisk As Thermostabil protease
DK145990D0 (da) * 1990-06-15 1990-06-15 Novo Nordisk As Thermostabil protease
US5283179A (en) 1990-09-10 1994-02-01 Promega Corporation Luciferase assay method
WO1992006188A2 (en) * 1990-10-05 1992-04-16 Barnes Wayne M Thermostable dna polymerase
US5545552A (en) * 1990-12-03 1996-08-13 Stratagene Purified thermostable pyrococcus furiosus DNA polymerase I
US5120349A (en) * 1990-12-07 1992-06-09 Landec Labs, Inc. Microcapsule having temperature-dependent permeability profile
US5580573A (en) 1991-02-01 1996-12-03 E. R. Squibb And Sons, Inc. Temperature activated controlled release
CH680325B5 (de) * 1991-02-25 1993-02-15 Ebauchesfabrik Eta Ag
US5229285A (en) * 1991-06-27 1993-07-20 Kikkoman Corporation Thermostable luciferase of firefly, thermostable luciferase gene of firefly, novel recombinant dna, and process for the preparation of thermostable luciferase of firefly
US5459055A (en) * 1991-12-27 1995-10-17 Epicentre Technologies Corporation Thermostable ribonuclease H isolated from Thermus flavus
US5413924A (en) 1992-02-13 1995-05-09 Kosak; Kenneth M. Preparation of wax beads containing a reagent for release by heating
EP0572057A1 (de) 1992-05-11 1993-12-01 Johnson & Johnson Clinical Diagnostics, Inc. PCR-Reagenzien- und Testsatz und Verfahren für die Amplifikation und Detektion mit verringerter, nicht-spezifischer Amplifikation von Nukleinsäuren
US5700672A (en) * 1992-07-23 1997-12-23 Stratagene Purified thermostable pyrococcus furiousus DNA ligase
US5744345A (en) * 1992-10-05 1998-04-28 Takara Shuzo Co., Ltd. Hyperthermostable β-galactosidase gene, enzyme encoded thereby, and process for production
US5565339A (en) * 1992-10-08 1996-10-15 Hoffmann-La Roche Inc. Compositions and methods for inhibiting dimerization of primers during storage of polymerase chain reaction reagents
US5332774A (en) * 1992-10-16 1994-07-26 Arco Chemical Technology, L.P. Polyvinyl acetal resins based on hydroxyaldehydes and use of the resins in laminar structures
US5614402A (en) * 1992-12-07 1997-03-25 Third Wave Technologies, Inc. 5' nucleases derived from thermostable DNA polymerase
CA2151396C (en) * 1992-12-23 2006-08-22 I.M.A. Industria Macchine Automatiche S.P.A. Process for preparing controlled release pharmaceutical forms and the forms thus obtained
US5436149A (en) * 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US5616402A (en) * 1993-03-19 1997-04-01 Ncr Corporation Printing ribbon for printing red scannable bar codes
US5427928A (en) * 1993-03-24 1995-06-27 Slesarev; Alexei I. Thermostable DNA Topoisomerase V
DE69414921T3 (de) * 1993-06-29 2004-04-15 Hewlett-Packard Co. (N.D.Ges.D.Staates Delaware), Palo Alto Vernetztes Polyvinylbutyral Bindemittel für organische Photoleiter
KR100262258B1 (ko) 1994-04-01 2000-07-15 다니엘 엘. 캐시앙, 헨리 엘. 노르호프, 피터 알. 쉬어리 고도로 정제된 재조합 역전사효소
US5939257A (en) * 1994-04-18 1999-08-17 Amersham Life Science, Inc. Thermostable alkaline phosphatases
WO1995030756A1 (en) * 1994-04-18 1995-11-16 United States Biochemical Corporation Thermostable alkaline phosphatase of thermus thermophilus
FR2719417B1 (fr) 1994-04-28 1996-07-19 Person Henri Le Composant à hétérostructure semi-conductrice, commande par la lumière pour la génération d'oscillations hyperfréquences.
US5643754A (en) * 1994-05-25 1997-07-01 The Regents Of The University Of California Nucleic acids encoding a leptospira outer membrane protein
JP3557289B2 (ja) * 1994-07-21 2004-08-25 株式会社林原生物化学研究所 非還元性糖質からトレハロースを遊離する組換え型耐熱性酵素
US5660854A (en) * 1994-11-28 1997-08-26 Haynes; Duncan H Drug releasing surgical implant or dressing material
AUPN245295A0 (en) * 1995-04-13 1995-05-11 Johnson & Johnson Research Pty. Limited Assay for genetic abnormalities
US5776720A (en) * 1995-05-18 1998-07-07 Coulter Corporation Assay reagent
US6033859A (en) * 1996-05-24 2000-03-07 Toyo Boseki Kabushiki Kaisha Thermostable DNA polymerase from a hyperthermophilic archaeon strain KOD1
JP3112148B2 (ja) * 1995-05-31 2000-11-27 東洋紡績株式会社 核酸の増幅方法およびその試薬
US5773258A (en) * 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
US5665551A (en) * 1995-09-13 1997-09-09 Roche Molecular Systems, Inc. Purified nucleic acid encoding a thermostable pyrophosphatase
ATE203056T1 (de) * 1995-12-15 2001-07-15 Amersham Life Science Inc Thermostabile dna polymerase aus thermoanaerobacter thermohydrosulfuricus und davon abgeleitete mutierte enzyme mit entfernter exonukleaseaktivität
WO1997027441A1 (en) * 1996-01-25 1997-07-31 Frazier-Simplex, Inc. Heat regeneration for oxy-fuel fired furnaces
US20020164751A1 (en) 1996-06-19 2002-11-07 Short Jay M. Enzymes having thermostable phosphatase activity and methods of use thereof
US6312892B1 (en) * 1996-07-19 2001-11-06 Cornell Research Foundation, Inc. High fidelity detection of nucleic acid differences by ligase detection reaction
DE69725076T2 (de) 1996-07-29 2004-04-15 Toyo Boseki K.K. Modifizierte thermostabile DNA Polymerase und eine DNA Polymerasezusammensetzung zur Amplifikation von Nukleinsäuren
US5736373A (en) * 1996-09-23 1998-04-07 Becton, Dickinson And Company Thermostable DNA polymerase from Bacillus pallidus
DE19641064A1 (de) * 1996-10-04 1998-04-09 Wacker Chemie Gmbh Modifizierte Polyvinylbutyrale mit niederer Lösungsviskosität
US5705379A (en) * 1996-10-23 1998-01-06 Cornell Research Foundation, Inc. Nucleotide sequences encoding a thermostable alkaline protease
FR2756560A1 (fr) * 1996-12-04 1998-06-05 Adir Nouveaux derives de l'imidazoline, leur procede de preparation et les compositions pharmaceutiques qui les contiennent
US6143517A (en) * 1996-12-23 2000-11-07 University Of Florida Thermostable proteolytic enzymes and uses thereof in peptide and protein synthesis
US5744150A (en) * 1997-01-29 1998-04-28 Xomed Surgical Products, Inc. Softened antimicrobial sponge material with color change indication of antimicrobial activity
JP3601835B2 (ja) * 1997-06-10 2004-12-15 タカラバイオ株式会社 超耐熱性プロテアーゼ発現系
US6113947A (en) * 1997-06-13 2000-09-05 Genentech, Inc. Controlled release microencapsulated NGF formulation
US6004752A (en) * 1997-07-29 1999-12-21 Sarnoff Corporation Solid support with attached molecules
JP3018163B2 (ja) * 1997-09-04 2000-03-13 工業技術院長 パイロコッカス属に属する超好熱性細菌由来の耐熱性Ｆｌａｐエンドヌクレアーゼ
CA2243985C (en) * 1997-09-11 2004-08-10 F. Hoffmann-La Roche Ag Thermostable dna polymerases incorporating nucleoside triphosphates labeled with fluorescein family dyes
AU754312C (en) * 1997-09-19 2005-11-10 Promega Corporation Thermostable luciferases and methods of production
US6602677B1 (en) 1997-09-19 2003-08-05 Promega Corporation Thermostable luciferases and methods of production
US6183998B1 (en) * 1998-05-29 2001-02-06 Qiagen Gmbh Max-Volmer-Strasse 4 Method for reversible modification of thermostable enzymes
US6130045A (en) * 1998-06-11 2000-10-10 Clontech Laboratories, Inc. Thermostable polymerase
US6294367B1 (en) * 1998-06-15 2001-09-25 California Institute Of Technology Thermostable peptidase
JP3482140B2 (ja) * 1998-10-06 2003-12-22 株式会社東芝 核酸鎖合成方法、核酸固定化チップおよび核酸鎖検出方法
AU6228799A (en) * 1998-10-26 2000-05-15 Tanabe Seiyaku Co., Ltd. Sustained-release particles
US6472431B2 (en) * 1998-12-23 2002-10-29 Orphan Medical, Inc. Microbiologically sound and stable solutions of gamma-hydroxybutyrate salt for the treatment of narcolepsy
US6380380B1 (en) * 1999-01-04 2002-04-30 Specialty Assays, Inc. Use of nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucliotide phosphate (NADP) analogs to measure enzyme activities metabolites and substrates
JP4175746B2 (ja) * 1999-08-30 2008-11-05 独立行政法人科学技術振興機構 新規な耐熱性コラーゲン分解酵素、前記酵素を産生する新規な微生物および前記酵素の製造方法
US6541216B1 (en) * 1999-12-22 2003-04-01 Roche Diagnostics Corporation Amperometric biosensor test strip
US7078208B2 (en) * 2000-05-26 2006-07-18 Invitrogen Corporation Thermostable reverse transcriptases and uses thereof
US6214557B1 (en) * 2000-06-06 2001-04-10 Washington University Cold sensitive mutant DNA polymerases
KR100392501B1 (ko) * 2000-06-28 2003-07-22 동국제약 주식회사 다중 에멀젼법에 의한 서방출성 미립구의 제조방법
US6576109B1 (en) * 2000-09-27 2003-06-10 Wealtec Enterprise Co., Ltd. Gel tray structure of an electrophoresis trough
ES2320415T3 (es) * 2000-12-21 2009-05-22 Unilever N.V. Composiciones limpiadoras.
JP4146095B2 (ja) * 2001-01-15 2008-09-03 ユニチカ株式会社 耐熱性グルコキナーゼ遺伝子、それを含有する組換えベクター、その組換えベクターを含有する形質転換体及びその形質転換体を用いた耐熱性グルコキナーゼの製造方法
WO2002092813A1 (en) * 2001-05-11 2002-11-21 Matsushita Electric Industrial Co., Ltd. Biomolecular substrate and method and apparatus for examination and diagnosis using the same
EP1275735A1 (de) * 2001-07-11 2003-01-15 Roche Diagnostics GmbH Zusammensetzung und Methode zur 'Hot start' Nukleinsäureamplifizierung
WO2003012066A2 (en) 2001-08-02 2003-02-13 Barnes Wayne M Magnesium precipitate hot start method for molecular manipulation of nucleic acids
US20030027196A1 (en) * 2001-08-02 2003-02-06 Barnes Wayne M. Magnesium precipitate methods for magnesium dependent enzymes
US6403341B1 (en) * 2001-08-02 2002-06-11 Wayne M. Barnes Magnesium precipitate hot start method for PCR
AU2002355578A1 (en) * 2001-08-10 2003-02-24 Board Of Regents, The University Of Texas System Thermostable uvra and uvrb polipeptides and methods of use
US6902921B2 (en) * 2001-10-30 2005-06-07 454 Corporation Sulfurylase-luciferase fusion proteins and thermostable sulfurylase
RU2215037C1 (ru) 2002-04-15 2003-10-27 Институт биоорганической химии им. М.М. Шемякина и Ю.А.Овчинникова РАН Способ ферментативного синтеза днк

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Publication number Publication date
US7306929B2 (en) 2007-12-11
ES2310745T3 (es) 2009-01-16
JP2006521827A (ja) 2006-09-28
CA2521476A1 (en) 2004-10-21
AU2004227368B2 (en) 2009-01-08
WO2004090169A2 (en) 2004-10-21
EP1611257B1 (de) 2008-08-20
US20060194209A1 (en) 2006-08-31
EP1611257A2 (de) 2006-01-04
WO2004090169A3 (en) 2005-03-24
AU2004227368A1 (en) 2004-10-21
DE10315640A1 (de) 2004-10-14
DE602004015958D1 (de) 2008-10-02
US20080138879A1 (en) 2008-06-12

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