ATE407202T1 - Enzymvarianten von subtilisin (subtilasen) - Google Patents

Enzymvarianten von subtilisin (subtilasen)

Info

Publication number
ATE407202T1
ATE407202T1 AT04731310T AT04731310T ATE407202T1 AT E407202 T1 ATE407202 T1 AT E407202T1 AT 04731310 T AT04731310 T AT 04731310T AT 04731310 T AT04731310 T AT 04731310T AT E407202 T1 ATE407202 T1 AT E407202T1
Authority
AT
Austria
Prior art keywords
subtilases
subtilisin
enzyme variants
compositions
egg stains
Prior art date
Application number
AT04731310T
Other languages
English (en)
Inventor
Stefan Minning
Juergen Knoetzel
Niels Henrik Soerensen
Jon Ness
Mark Welch
Lorraine Giver
Joel Cherry
Borchert Torben Vedel
Jeremy Minshull
Original Assignee
Novozymes As
Maxygen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As, Maxygen Inc filed Critical Novozymes As
Application granted granted Critical
Publication of ATE407202T1 publication Critical patent/ATE407202T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38636—Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38681—Chemically modified or immobilised enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/582—Recycling of unreacted starting or intermediate materials

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
AT04731310T 2003-05-07 2004-05-06 Enzymvarianten von subtilisin (subtilasen) ATE407202T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DKPA200300690 2003-05-07

Publications (1)

Publication Number Publication Date
ATE407202T1 true ATE407202T1 (de) 2008-09-15

Family

ID=33426922

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04731310T ATE407202T1 (de) 2003-05-07 2004-05-06 Enzymvarianten von subtilisin (subtilasen)

Country Status (10)

Country Link
US (1) US7507569B2 (de)
EP (1) EP1623029B1 (de)
JP (1) JP4819670B2 (de)
CN (2) CN102766545B (de)
AT (1) ATE407202T1 (de)
AU (1) AU2004236308B2 (de)
BR (1) BRPI0409992A (de)
CA (1) CA2526341C (de)
DE (1) DE602004016314D1 (de)
WO (1) WO2004099401A1 (de)

Families Citing this family (63)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6682924B1 (en) * 1995-05-05 2004-01-27 Novozymes A/S Protease variants and compositions
WO1999011768A1 (en) * 1997-08-29 1999-03-11 Novo Nordisk A/S Protease variants and compositions
CA2406621A1 (en) * 2000-04-28 2001-11-08 Novozymes A/S Protein variants having modified immunogenicity
JP2009534022A (ja) 2006-04-20 2009-09-24 ノボザイムス アクティーゼルスカブ 卵のシミに対する洗浄能力が向上したサビナーゼ変異体
WO2008010925A2 (en) * 2006-07-18 2008-01-24 Danisco Us, Inc., Genencor Division Protease variants active over a broad temperature range
ES2419234T5 (es) * 2006-10-06 2017-05-05 Novozymes A/S Composiciones detergentes y uso de combinaciones de enzimas en las mismas
DE102007038031A1 (de) * 2007-08-10 2009-06-04 Henkel Ag & Co. Kgaa Mittel enthaltend Proteasen
DE102007044415A1 (de) * 2007-09-17 2009-03-19 Henkel Ag & Co. Kgaa Leistungsverbesserte Proteasen und Wasch- und Reinigungsmittel enthaltend diese Proteasen
US7781387B2 (en) * 2008-01-22 2010-08-24 Access Business Group International, Llc. Automatic phosphate-free dishwashing detergent providing improved spotting and filming performance
US20110034367A1 (en) 2008-02-01 2011-02-10 Novozymes A/S Liquid Enzyme Composition
MX2010010348A (es) 2008-03-26 2010-11-09 Novozymes As Composiciones de enzimas liquidas estabilizadas.
WO2010056635A1 (en) * 2008-11-11 2010-05-20 Danisco Us Inc. Compositions and methods comprising a subtilisin variant
GB0915572D0 (en) * 2009-09-07 2009-10-07 Reckitt Benckiser Nv Detergent composition
MX339581B (es) * 2009-09-25 2016-06-01 Novozymes As Variantes de subtilasa.
IN2014CN00700A (de) 2011-07-01 2015-04-03 Novozymes As
WO2013004635A1 (en) 2011-07-01 2013-01-10 Novozymes A/S Liquid detergent composition
DK2734633T3 (da) 2011-07-22 2019-06-11 Novozymes North America Inc Fremgangsmåder til forbehandling af celluloseholdigt materiale og forbedring af hydrolyse deraf
DE102011118027A1 (de) * 2011-09-12 2013-03-14 Henkel Ag & Co. Kgaa Verfahren zur Anpassung eines Hydrolytischen Enzyms an eine das hydrolytische Enzym stabilisierende Komponente
EP2782988A1 (de) 2011-11-25 2014-10-01 Novozymes A/S Subtilasevarianten und dafür codierende polynucleotide
EP2607468A1 (de) 2011-12-20 2013-06-26 Henkel AG & Co. KGaA Reinigungsmittelzusammensetzungen mit Subtilasevarianten
CN104011204A (zh) 2011-12-20 2014-08-27 诺维信公司 枯草杆菌酶变体和编码它们的多核苷酸
MX2014008764A (es) 2012-01-26 2014-08-27 Novozymes As Uso de polipeptidos que tienen actividad proteasa en alimentos para animales y detergentes.
EP2814956B1 (de) 2012-02-17 2017-05-10 Novozymes A/S Subtilisinvarianten und polynukleotide zu deren codierung
EP2628785B1 (de) 2012-02-17 2016-05-18 Henkel AG & Co. KGaA Reinigungsmittelzusammensetzungen mit Subtilasevarianten
US9458441B2 (en) 2012-05-07 2016-10-04 Novozymes A/S Polypeptides having xanthan degrading activity and polynucleotides encoding same
EP2970830B1 (de) 2013-03-14 2017-12-13 Novozymes A/S Protease und hemmer enthaltend in wasserlöslichen filmen
EP3569611A1 (de) 2013-04-23 2019-11-20 Novozymes A/S Flüssige reinigungszusammensetzungen für automatische geschirrspüler
EP2992076B1 (de) 2013-05-03 2018-10-24 Novozymes A/S Mikroverkapselung von wasch und reinigungsmittelenzymen
EP2832853A1 (de) 2013-07-29 2015-02-04 Henkel AG&Co. KGAA Waschmittelzusammensetzung mit Proteasevarianten
US9613906B2 (en) * 2014-06-23 2017-04-04 GlobalFoundries, Inc. Integrated circuits including modified liners and methods for fabricating the same
WO2016001319A1 (en) 2014-07-03 2016-01-07 Novozymes A/S Improved stabilization of non-protease enzyme
WO2016079305A1 (en) 2014-11-20 2016-05-26 Novozymes A/S Alicyclobacillus variants and polynucleotides encoding same
CA2975814C (en) 2015-03-30 2023-06-27 Nestec S.A. Milk-based protein hydrolysates and compositions made thereof
WO2016205755A1 (en) * 2015-06-17 2016-12-22 Danisco Us Inc. Bacillus gibsonii-clade serine proteases
WO2017117089A1 (en) 2015-12-28 2017-07-06 Novozymes Bioag A/S Heat priming of bacterial spores
EP3464538A1 (de) 2016-05-31 2019-04-10 Novozymes A/S Stabilisierte flüssige peroxidzusammensetzungen
US11072765B2 (en) 2016-08-24 2021-07-27 Novozymes A/S GH9 endoglucanase variants and polynucleotides encoding same
WO2018037065A1 (en) 2016-08-24 2018-03-01 Henkel Ag & Co. Kgaa Detergent composition comprising gh9 endoglucanase variants i
WO2018037061A1 (en) 2016-08-24 2018-03-01 Novozymes A/S Xanthan lyase variants and polynucleotides encoding same
AU2017317563B8 (en) 2016-08-24 2023-03-23 Henkel Ag & Co. Kgaa Detergent compositions comprising xanthan lyase variants I
US20190284647A1 (en) 2016-09-29 2019-09-19 Novozymes A/S Spore Containing Granule
EP3401385A1 (de) 2017-05-08 2018-11-14 Henkel AG & Co. KGaA Tensidzusammensetzungen enthaltend polypeptide enthaltend eine carbohydrate binding domain
WO2018206535A1 (en) 2017-05-08 2018-11-15 Novozymes A/S Carbohydrate-binding domain and polynucleotides encoding the same
EP3645692B1 (de) 2017-06-30 2025-12-31 Novozymes A/S Enzymschlammzusammensetzung
CA3071078A1 (en) 2017-08-24 2019-02-28 Novozymes A/S Xanthan lyase variants and polynucleotides encoding same
WO2019038058A1 (en) 2017-08-24 2019-02-28 Novozymes A/S GH9 ENDOGLUCANASE VARIANTS AND POLYNUCLEOTIDES ENCODING SUCH VARIANTS
EP3673060A1 (de) 2017-08-24 2020-07-01 Henkel AG & Co. KGaA Reinigungsmittelzusammensetzung mit xanthanlyasevarianten ii
US11624059B2 (en) 2017-08-24 2023-04-11 Henkel Ag & Co. Kgaa Detergent compositions comprising GH9 endoglucanase variants II
CA3073362A1 (en) 2017-09-27 2019-04-04 Novozymes A/S Lipase variants and microcapsule compositions comprising such lipase variants
WO2019143622A1 (en) 2018-01-19 2019-07-25 Bio-Rad Laboratories, Inc. Mutant dna polymerases
EP3749761A1 (de) 2018-02-08 2020-12-16 Novozymes A/S Lipasen, lipasevarianten und zusammensetzungen davon
CN111757930A (zh) 2018-02-08 2020-10-09 诺维信公司 脂肪酶变体及其组合物
US20210102184A1 (en) 2018-02-23 2021-04-08 Henkel Ag & Co. Kgaa Detergent composition comprising xanthan lyase and endoglucanase variants
US20210002588A1 (en) 2018-03-13 2021-01-07 Novozymes A/S Microencapsulation Using Amino Sugar Oligomers
US20210363470A1 (en) * 2018-06-19 2021-11-25 Danisco Us Inc Subtilisin variants
EP3994148A1 (de) 2019-07-01 2022-05-11 Basf Se Peptideacetale zur stabilisierung von enzymen
WO2021001400A1 (en) 2019-07-02 2021-01-07 Novozymes A/S Lipase variants and compositions thereof
EP3907271A1 (de) 2020-05-07 2021-11-10 Novozymes A/S Reinigungszusammensetzung, verwendung und verfahren zum reinigen
EP4039806A1 (de) 2021-02-04 2022-08-10 Henkel AG & Co. KGaA Reinigungsmittelzusammensetzung mit xanthan-lyase- und endoglucanase-varianten mit verbesserter stabilität
US20250311734A1 (en) 2022-05-14 2025-10-09 Novozymes A/S Compositions and Methods for Preventing, Treating, Suppressing and/or Eliminatting Phytopathogenic Infestations and Infections
CN120712348A (zh) * 2023-03-06 2025-09-26 丹尼斯科美国公司 枯草杆菌蛋白酶变体和使用方法
WO2025085351A1 (en) * 2023-10-20 2025-04-24 Danisco Us Inc. Subtilisin variants and methods of use
DE102024207057A1 (de) * 2024-07-26 2026-01-29 Henkel Ag & Co. Kgaa Protease-varianten mit verbesserter lagerstabilität

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ208612A (en) 1983-06-24 1991-09-25 Genentech Inc Method of producing "procaryotic carbonyl hydrolases" containing predetermined, site specific mutations
DE3527913A1 (de) 1985-08-03 1987-02-12 Henkel Kgaa Alkalische protease, verfahren zur herstellung von hybridvektoren und genetisch transformierte mikroorganismen
WO1987004461A1 (en) 1986-01-15 1987-07-30 Amgen THERMALLY STABLE AND pH STABLE SUBTILISIN ANALOGS AND METHOD FOR PRODUCTION THEREOF
WO1987005050A1 (en) 1986-02-12 1987-08-27 Genex Corporation Mutagenesis and screening method and product
US4990452A (en) * 1986-02-12 1991-02-05 Genex Corporation Combining mutations for stabilization of subtilisin
DE3750916T2 (de) 1986-04-30 1995-06-01 Genencor Int Mutante einer nicht menschlichen Carbonyl-Hydrolase, für diese kodierende DNS-Sequenzen und Vektoren und durch diese Vektoren transformierte Wirte.
NZ221627A (en) 1986-09-09 1993-04-28 Genencor Inc Preparation of enzymes, modifications, catalytic triads to alter ratios or transesterification/hydrolysis ratios
US5260207A (en) * 1987-04-06 1993-11-09 Enzon Labs Inc. Engineering of electrostatic interactions at metal ion binding sites for the stabilization of proteins
WO1988008028A1 (en) 1987-04-06 1988-10-20 Genex Corporation The engineering of electrostatic interactions at metal ion binding sites for the stabilization of proteins
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
DK6488D0 (da) 1988-01-07 1988-01-07 Novo Industri As Enzymer
US5543302A (en) 1988-05-27 1996-08-06 Solvay Enzymes, Inc. Proteases of altered stability to autolytic degradation
KR100188532B1 (ko) * 1989-05-17 1999-06-01 스티븐 엠. 오드리 돌연변이 섭티리신 및 그를 함유하는 조성물
US5665587A (en) * 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
DK316989D0 (da) 1989-06-26 1989-06-26 Novo Nordisk As Enzymer
US5340735A (en) * 1991-05-29 1994-08-23 Cognis, Inc. Bacillus lentus alkaline protease variants with increased stability
EP0995801A1 (de) 1991-07-27 2000-04-26 Genencor International GmbH Verfahren zur Verbesserung des Stabilität von Enzymen und stabilisierte Enzyme
AU682314B2 (en) 1992-07-17 1997-10-02 Genencor International, Inc. High alkaline serine proteases
US6436690B1 (en) 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
MA23346A1 (fr) * 1993-10-14 1995-04-01 Genencor Int Variantes de la subtilisine
DE69434962T2 (de) * 1993-10-14 2008-01-17 The Procter & Gamble Company, Cincinnati Proteasehaltige reinigungsmittel
ES2199978T3 (es) * 1993-10-14 2004-03-01 THE PROCTER & GAMBLE COMPANY Composiciones de blanqueo que comprenden enzimas proteasa.
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
DE4411223A1 (de) 1994-03-31 1995-10-05 Solvay Enzymes Gmbh & Co Kg Verwendung alkalischer Proteasen in gewerblichen Textilwaschverfahren
US6599730B1 (en) 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
ZA952220B (en) 1994-05-02 1995-12-14 Procter & Gamble Bpn' variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
US5837517A (en) * 1995-05-05 1998-11-17 Novo Nordisk A/S Protease variants and compositions
DE69637907D1 (de) 1995-05-05 2009-06-04 Novozymes As Protease-varianten und verbindungen
US6777218B1 (en) * 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
US6902922B2 (en) * 2000-04-03 2005-06-07 Novozymes A/S Subtilisin variants
WO2002042740A1 (en) 2000-11-27 2002-05-30 Novozymes A/S Automated mechanical stress assay for screening cleaning ingredients
DK200101090A (da) * 2001-07-12 2001-08-16 Novozymes As Subtilase variants
DE10162728A1 (de) * 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14393) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease

Also Published As

Publication number Publication date
WO2004099401A1 (en) 2004-11-18
US7507569B2 (en) 2009-03-24
AU2004236308B2 (en) 2010-09-09
US20070015677A1 (en) 2007-01-18
DE602004016314D1 (de) 2008-10-16
JP2006524998A (ja) 2006-11-09
BRPI0409992A (pt) 2006-05-09
CA2526341A1 (en) 2004-11-18
AU2004236308A1 (en) 2004-11-18
CA2526341C (en) 2013-02-19
EP1623029B1 (de) 2008-09-03
CN1784490A (zh) 2006-06-07
CN102766545A (zh) 2012-11-07
CN102766545B (zh) 2014-08-06
JP4819670B2 (ja) 2011-11-24
EP1623029A1 (de) 2006-02-08

Similar Documents

Publication Publication Date Title
DE602004016314D1 (de) Enzymvarianten von subtilisin (subtilasen)
WO2001068821A3 (en) Novel subtilase enzymes having an improved wash performance on egg stains
ATE409744T1 (de) Subtilasevarianten
TR200002013T2 (tr) Çok sayıda ikameli proteaz çeşidi ihtiva eden temizlik bileşimleri.
ATE516347T1 (de) Protease mit verbesserter stabilität in detergentien
BR9607127A (pt) Composições para lavagem automática de pratos compreendendo catalísadores de cobalto(III)
BR9607008A (pt) Composições de lavagem de prato automática compreendendo catalísadores quelatados por cobalto
DK2295554T3 (da) Perhydrolase
EP2138574A3 (de) Subtilase Varianten
BR9917144A (pt) Composições para lavagem de louçacompreendendo alquilbenzeno sulfonatosmodificados
DK1414977T3 (da) Vaske- og rengöringsmiddel med hybride alfa-amylaser
WO2004074419A3 (en) Detergent compositions
ATE277157T1 (de) Reinigungsverfahren zur stärkeentfernung
HUP0300967A2 (hu) Ciklodextrin glukanotranszferáz enzimet tartalmazó mosószerkészítmények
DE602005026541D1 (de) Subtilasen
DE69331907D1 (de) Neue alkalische proteasen enthaltende reinigungsmittelzusammensetzungen
BR0012090A (pt) Composições detergentes compreendendo uma enzima amiloglicosidase
DK1539910T3 (da) Rengöringsmiddel indeholdende en hydrofilicerende polymer
DE60117026D1 (de) Verbesserungen in enzyme enthaltende tabletten
DE60227177D1 (de) Reinigungsmittel enthaltend ein hydrophilisierendes polymer
BR9713171A (pt) Composições detergentes compreendendo atividade enzimática controlada por anticorpo
BRPI0415351A (pt) composição detergente compreendendo partìcula de alvejante revestida
BR9710965A (pt) Detergentes contendo enzima protease e enzima loccase
MY199664A (en) Enzyme-based detergent composition
TR199900272T2 (xx) Alkalin pektin parçalayan enzim ihtiva eden deterjan bileşimleri.

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties