ATE414146T1 - Methoden zur herstellung von helpervirusfreien präparationen von freien rekombinenten aav- vektoren mit hohem titer - Google Patents

Methoden zur herstellung von helpervirusfreien präparationen von freien rekombinenten aav- vektoren mit hohem titer

Info

Publication number
ATE414146T1
ATE414146T1 AT99968678T AT99968678T ATE414146T1 AT E414146 T1 ATE414146 T1 AT E414146T1 AT 99968678 T AT99968678 T AT 99968678T AT 99968678 T AT99968678 T AT 99968678T AT E414146 T1 ATE414146 T1 AT E414146T1
Authority
AT
Austria
Prior art keywords
free
producing
helpervirus
methods
aav vectors
Prior art date
Application number
AT99968678T
Other languages
English (en)
Inventor
Edward Atkinson
Victor Fung
Perry Wilkins
Ryan Takeya
Thomas Reynolds
Original Assignee
Targeted Genetics Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Targeted Genetics Corp filed Critical Targeted Genetics Corp
Application granted granted Critical
Publication of ATE414146T1 publication Critical patent/ATE414146T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material
    • C12N2750/14152—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Virology (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Chemical Or Physical Treatment Of Fibers (AREA)
AT99968678T 1998-09-04 1999-09-07 Methoden zur herstellung von helpervirusfreien präparationen von freien rekombinenten aav- vektoren mit hohem titer ATE414146T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US14247498A 1998-09-04 1998-09-04
US12368599P 1999-03-10 1999-03-10

Publications (1)

Publication Number Publication Date
ATE414146T1 true ATE414146T1 (de) 2008-11-15

Family

ID=26821785

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99968678T ATE414146T1 (de) 1998-09-04 1999-09-07 Methoden zur herstellung von helpervirusfreien präparationen von freien rekombinenten aav- vektoren mit hohem titer

Country Status (10)

Country Link
EP (1) EP1109892B1 (de)
JP (1) JP4472182B2 (de)
AT (1) ATE414146T1 (de)
CA (1) CA2342849C (de)
CY (1) CY1110695T1 (de)
DE (1) DE69939903D1 (de)
DK (1) DK1109892T3 (de)
ES (2) ES2541540T3 (de)
PT (2) PT1109892E (de)
WO (1) WO2000014205A2 (de)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6924128B2 (en) 1994-12-06 2005-08-02 Targeted Genetics Corporation Packaging cell lines for generation of high titers of recombinant AAV vectors
US6989264B2 (en) 1997-09-05 2006-01-24 Targeted Genetics Corporation Methods for generating high titer helper-free preparations of released recombinant AAV vectors
WO1999011764A2 (en) * 1997-09-05 1999-03-11 Targeted Genetics Corporation Methods for generating high titer helper-free preparations of recombinant aav vectors
US6566118B1 (en) 1997-09-05 2003-05-20 Targeted Genetics Corporation Methods for generating high titer helper-free preparations of released recombinant AAV vectors
US6346415B1 (en) 1997-10-21 2002-02-12 Targeted Genetics Corporation Transcriptionally-activated AAV inverted terminal repeats (ITRS) for use with recombinant AAV vectors
US6893865B1 (en) 1999-04-28 2005-05-17 Targeted Genetics Corporation Methods, compositions, and cells for encapsidating recombinant vectors in AAV particles
EP1939300A1 (de) 1999-05-28 2008-07-02 Targeted Genetics Corporation Verfahren und Zusammensetzung zur Senkung des Spiegels des Tumornekrosefaktors (TNF) bei TNF-assoziierten Leiden
JP2003501043A (ja) 1999-05-28 2003-01-14 ターゲティッド ジェネティクス コーポレイション 腫瘍壊死因子（ｔｎｆ）関連障害において、ｔｎｆのレベルを低下させるための方法および組成物
ATE403715T1 (de) 1999-08-09 2008-08-15 Targeted Genetics Corp Erhöhung der expression einer einzelsträngigen, heterologen nukleotidsequenz von einem rekombinanten viralen vektor durch ausgestaltung der sequenz in einer art und weise, dass basenpaarungen innerhalb der sequenz entstehen
US7115391B1 (en) 1999-10-01 2006-10-03 Genovo, Inc. Production of recombinant AAV using adenovirus comprising AAV rep/cap genes
WO2006044594A2 (en) * 2004-10-15 2006-04-27 Monell Chemical Senses Center Methods for culturing mammalian taste cells
WO2006052813A2 (en) * 2004-11-05 2006-05-18 Wellstat Biologics Corporation Stable and filterable enveloped virus formulations
SG10202109219SA (en) 2009-05-02 2021-10-28 Genzyme Corp Gene therapy for neurodegenerative disorders
CA2829774C (en) * 2011-03-14 2019-09-24 National Research Council Of Canada Method of viral production in cells
WO2015005430A1 (ja) 2013-07-11 2015-01-15 タカラバイオ株式会社 非エンベロープウイルスの製造方法
EP3168298B1 (de) 2014-07-10 2019-04-17 Takara Bio Inc. Herstellungsverfahren für nichtumhüllte viruspartikel
KR102493350B1 (ko) 2015-01-09 2023-01-27 다카라 바이오 가부시키가이샤 비엔벨로프 바이러스 입자의 제조방법
WO2017205573A1 (en) * 2016-05-25 2017-11-30 Lonza Houston Inc. Methods for isolating adeno-associated virus using a polydiallyldialkylammonium salt
ES3012649T3 (en) * 2016-10-14 2025-04-09 Ultragenyx Pharmaceutical Inc Use of tonicifying agents to enhance recombinant adeno-associated virus yield
WO2023114901A2 (en) * 2021-12-15 2023-06-22 Oxford Biomedica Solutions Llc Methods and compositions for the production of adeno-associated virus
CN120835797A (zh) 2023-03-21 2025-10-24 豪夫迈·罗氏有限公司 用于产生重组aav颗粒制备物的方法
WO2025168663A1 (en) 2024-02-09 2025-08-14 F. Hoffmann-La Roche Ag Method for producing recombinant adeno-associated viral particles
US20260022401A1 (en) 2024-07-22 2026-01-22 Hoffmann-La Roche Inc. Novel AAV rep ORFs and Rep polypeptides

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6686200B1 (en) * 1993-08-31 2004-02-03 Uab Research Foundation Methods and compositions for the large scale production of recombinant adeno-associated virus
AU722196B2 (en) * 1995-08-30 2000-07-27 Genzyme Corporation Chromatographic purification of adenovirus and AAV
US5910434A (en) * 1995-12-15 1999-06-08 Systemix, Inc. Method for obtaining retroviral packaging cell lines producing high transducing efficiency retroviral supernatant
FR2750433B1 (fr) * 1996-07-01 1998-08-14 Rhone Poulenc Rorer Sa Procede de production d'adenovirus recombinants
WO1999011764A2 (en) * 1997-09-05 1999-03-11 Targeted Genetics Corporation Methods for generating high titer helper-free preparations of recombinant aav vectors

Also Published As

Publication number Publication date
ES2317709T3 (es) 2009-04-16
ES2541540T3 (es) 2015-07-21
PT1109892E (pt) 2009-02-17
JP4472182B2 (ja) 2010-06-02
PT1930418E (pt) 2015-08-26
EP1109892B1 (de) 2008-11-12
DK1109892T3 (da) 2009-02-09
DE69939903D1 (de) 2008-12-24
WO2000014205A9 (en) 2000-09-08
EP1109892A2 (de) 2001-06-27
WO2000014205A2 (en) 2000-03-16
CY1110695T1 (el) 2015-06-10
CA2342849C (en) 2013-01-29
JP2002524070A (ja) 2002-08-06
CA2342849A1 (en) 2000-03-16
WO2000014205A3 (en) 2000-05-25

Similar Documents

Publication Publication Date Title
ATE414146T1 (de) Methoden zur herstellung von helpervirusfreien präparationen von freien rekombinenten aav- vektoren mit hohem titer
ATE542891T1 (de) Verfahren zur herstellung von biologischen produkten in protein-freier kultur
DK0990044T3 (da) En fremgangsmåde til en vitro-molekylær evolution af proteinfunktion
ATE549347T1 (de) Gene von säugetiere, und damit verbundene reagentien, methoden
DE69938302D1 (de) Neisseria meningitidis antigene und zusammenstellungen
DE69111440D1 (de) Methoden und zusammensetzungen zur impfung gegen hiv.
DE69737023D1 (de) Methode zur behandlung von krebs und pathogenen infektionen unter verwendung von antigen-präsentierenden zellen beladen mit rna
ATE410441T1 (de) Zusammensetzungen und methoden zur behandlung von infektionen unter verwendung von indolicidinanalogen
CY1111831T1 (el) Παραγωγα ογκο-συναφους αντιγονου απο την οικογενεια mage και αλληλουχιες νουκλεϊνικου οξεος που κωδικοποιουν αυτα, που χρησιμοποιουνται για την παρασκευη πρωτεϊνων συντηξης και συνθεσεων για εμβολιασμο
DE69941823D1 (de) Eine verbesserte methode zur herstellung und reinigung von adenoviralen vektoren
DE59410380D1 (de) Endosomolytisch wirksame partikel
PL409838A1 (pl) Rekombinowane wirusy stowarzyszone z adenowirusem (AAV), kompozycje , wyizolowane białko kapsydu, wyizolowana lub syntetyczna cząsteczka kwasu nukleinowego, sposób wytwarzania rekombinowanych wirusów, komórki gospodarza, białka obejmujące fragment białka kapsydu AAV, sztuczne białka, rekombinowane wirusy, cząsteczki, sposoby dostarczania transgenu do komórki , sposób identyfikacji serotypu sekwencji wirusa (AAV), zestaw diagnostyczny, sposób izolacji nowych wirusów, nowe serotypy wirusa, wyizolowane wirusy, rekombinowana komórka, zastosowanie wirusa
ATE454445T1 (de) Virusvektoren und verfahren für ihre herstellung und verabreichung.
DE60134136D1 (de) Zytokinrezeptoruntereinheit-proteine aus säugetieren, damit zusammenhängende reagenzien und methoden
DE50112802D1 (de) Peptide, deren herstellung und verwendung zur bindung von immunglobulinen
DE69839403D1 (de) Adenovirale vektoren und eine methode zur reduktion von homologer rekombination
CY1106111T1 (el) Ανασυνδυασμενος σταθepος κυτταρικος κλωνος, η παρασκευη και χρησιμοποιηση του
WO2001032882A3 (en) Nucleic acids and proteins from group b streptococcus
AR022150A1 (es) Acidos nucleicos derivados de monocitos y composiciones y metodos relacionados
ATE435034T1 (de) Expression und charakterisierung eines hiv-1 hüllproteins, das mit einer breitreaktiven neutralisierenden antikörperantwort assoziiert ist.
CY1116753T1 (el) Μεθοδοι για την παραγωγη παρασκευασματων υψηλου τιτλου χωρις βοηθους των αποδεσμευμενων ανασυνδυασμενων φορεων aav
WO2002077648A3 (en) Pathogenic and commensal vaccine antigens
SE9604439D0 (sv) New receptor
CU23097A1 (es) Método para la obtención de líneas celulares en medio libre de proteína y líneas celulares obtenidas por este método

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1109892

Country of ref document: EP