ATE418611T1 - Sirna-gesteuerte genexpressionsunterdrückung in transgenetischen tieren - Google Patents

Sirna-gesteuerte genexpressionsunterdrückung in transgenetischen tieren

Info

Publication number
ATE418611T1
ATE418611T1 AT03770964T AT03770964T ATE418611T1 AT E418611 T1 ATE418611 T1 AT E418611T1 AT 03770964 T AT03770964 T AT 03770964T AT 03770964 T AT03770964 T AT 03770964T AT E418611 T1 ATE418611 T1 AT E418611T1
Authority
AT
Austria
Prior art keywords
transgenetic
sirna
animals
gene expression
expression suppression
Prior art date
Application number
AT03770964T
Other languages
English (en)
Inventor
Jost Seibler
Frieder Schwenk
Ralf Kuehn
Birgit Kueter-Luks
Original Assignee
Taconicartemis Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Taconicartemis Gmbh filed Critical Taconicartemis Gmbh
Application granted granted Critical
Publication of ATE418611T1 publication Critical patent/ATE418611T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A01K2217/054—Animals comprising random inserted nucleic acids (transgenic) inducing loss of function
    • A01K2217/058—Animals comprising random inserted nucleic acids (transgenic) inducing loss of function due to expression of inhibitory nucleic acid, e.g. siRNA, antisense
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/20—Animal model comprising regulated expression system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/40—Fish
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Veterinary Medicine (AREA)
  • Environmental Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
AT03770964T 2002-10-17 2003-10-10 Sirna-gesteuerte genexpressionsunterdrückung in transgenetischen tieren ATE418611T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP02023283 2002-10-17
US46781403P 2003-05-02 2003-05-02
US48596903P 2003-07-10 2003-07-10

Publications (1)

Publication Number Publication Date
ATE418611T1 true ATE418611T1 (de) 2009-01-15

Family

ID=32110519

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03770964T ATE418611T1 (de) 2002-10-17 2003-10-10 Sirna-gesteuerte genexpressionsunterdrückung in transgenetischen tieren

Country Status (7)

Country Link
US (1) US20060218654A1 (de)
EP (1) EP1551971B1 (de)
AT (1) ATE418611T1 (de)
AU (1) AU2003280356A1 (de)
CA (1) CA2502664A1 (de)
DE (1) DE60325494D1 (de)
WO (1) WO2004035782A2 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8198077B2 (en) * 2003-01-17 2012-06-12 Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. Inducible small RNA expression constructs for targeted gene silencing
GB0327585D0 (en) * 2003-11-27 2003-12-31 Genomica Sau Inducible nucleic acid expression system
EP1614755A1 (de) * 2004-07-07 2006-01-11 ARTEMIS Pharmaceuticals GmbH Gezielte Transgenese von Expressionskassetten für shRNA(short hairpin RNA) mittels einer Rekombinasegesteuerte Kassettenumtausch
US8137907B2 (en) 2005-01-03 2012-03-20 Cold Spring Harbor Laboratory Orthotopic and genetically tractable non-human animal model for liver cancer and the uses thereof
EP1891219A2 (de) * 2005-05-31 2008-02-27 The Centre National de la Recherche Scientifique Tetracyclin-abhängige regulation von rna-interferenzen
EP1896587A2 (de) 2005-05-31 2008-03-12 Cold Spring Harbor Laboratory Verfahren zur mikro-rna-herstellung
US20090210955A1 (en) * 2005-06-09 2009-08-20 Artemis Pharmaceuticals Gmbh Shrna and sirna expression in a living organism under control of a codon-optimized repressor gene
EP1731607A1 (de) * 2005-06-09 2006-12-13 ARTEMIS Pharmaceuticals GmbH shRNA und siRNA Expression in einem lebenden Organismus unter Kontrolle eines Codon-optimierten Tetracycline-Repressorgenes
US20080220471A1 (en) * 2005-07-27 2008-09-11 Genentech, Inc. Vectors and Methods Using Same
WO2012037547A2 (en) * 2010-09-17 2012-03-22 Mount Sinai School Of Medicine Methods and compositions for inhibiting autophagy for the treatment of fibrosis
WO2014193489A1 (en) * 2013-05-31 2014-12-04 Brandeis University Organic small hairpin rnas
US20170226550A1 (en) * 2014-08-07 2017-08-10 Merck Sharp & Dohme Corp. Inhibitory rna for enhanced protein production in recombinant mammalian cells

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999053017A2 (en) * 1998-04-15 1999-10-21 Fred Hutchinson Cancer Research Center Methods and vector constructs for making transgenic non-human animals which ubiquitously express a heterologous gene
US20030084471A1 (en) * 2000-03-16 2003-05-01 David Beach Methods and compositions for RNA interference
EP1272630A2 (de) * 2000-03-16 2003-01-08 Genetica, Inc. Methoden und zusammensetzungen zur interferenz durch rna
US7294504B1 (en) * 2001-12-27 2007-11-13 Allele Biotechnology & Pharmaceuticals, Inc. Methods and compositions for DNA mediated gene silencing
US20080226553A1 (en) * 2002-09-27 2008-09-18 Cold Spring Harbor Laboratory Cell-Based Rna Interference and Related Methods and Compositions

Also Published As

Publication number Publication date
EP1551971A2 (de) 2005-07-13
AU2003280356A1 (en) 2004-05-04
CA2502664A1 (en) 2004-04-29
DE60325494D1 (de) 2009-02-05
WO2004035782A3 (en) 2004-05-21
US20060218654A1 (en) 2006-09-28
EP1551971B1 (de) 2008-12-24
WO2004035782A2 (en) 2004-04-29

Similar Documents

Publication Publication Date Title
ATE418611T1 (de) Sirna-gesteuerte genexpressionsunterdrückung in transgenetischen tieren
WO2002000894A3 (en) Gene silencing vector
ATE439440T1 (de) Sirna-vermitteltes gen-silencing von alpha- synuklein
WO2003080807A3 (en) Compositions and methods for suppressing eukaryotic gene expression
ATE490321T1 (de) Induzierbare sirna expressionskonstrukte zur gezielten genabschaltung
ATE536415T1 (de) Klonierung des cytochrome p450 gens von nicotiana
DK1546174T3 (da) Fremgangsmåder og sammensætninger til RNA-interferens
EP1606304A4 (de) Genexpressionsregulierende nukleotidsequenzen sowie konstrukte und verfahren unter verwendung davon
AU2001265756A1 (en) Sleeping beauty, a transposon vector with a broad host range for the genetic transfprmation in vertebrates
EP1913149A4 (de) Gezielte integration und expression exogener nukleinsäuresequenzen
GB2406169B (en) Methods and compositions relating to labeled rna molecules that reduce gene expression
EP1629104A4 (de) Zelloberflächenexpressionsvektor von sars-virus-antigen und damit transformierte mikroorganismen
ATE492631T1 (de) Verwendung von mutationen zur verbesserung von aspergillus-phytasen
ATE513929T1 (de) Gene mit einfluss auf die menschliche gedächtnisleistung
DK1339853T3 (da) Isolerede luciferaser og deres anvendelse
AU2003260200A8 (en) Reversible, parallel and multitask cloning method and kit
DK1664102T3 (da) Isoleret photoprotein mtclytin, samt dets anvendelse
NO20044901L (no) Anvendelse av modifiserte novirhabdoviruser i vaksiner
ATE338826T1 (de) Rekombinante virale vektoren zur tetracyclinregulierbaren genexpression
WO2003060092A3 (en) Modified fatty acid hydroxylase protein and genes
DK1587932T3 (da) Plantetransformation med in vivo samling af et træk
ATE520775T1 (de) Raygraspollen-spezifische promotoren und expressionskonstrukte
TW200519200A (en) Method of searching for functional nucleotide molecule
ATE349519T1 (de) Gen beteiligt an v(d)j rekombination und/oder dna reparatur
WO2006047635A3 (en) Methods and compositions for seprase inactivation

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties