ATE419329T1 - Verfahren zur kontrolle von polypeptidproduktion und -aufreinigung mit oberflächen verstärkter laserdesorption/ionisations massenspektrometrie - Google Patents

Verfahren zur kontrolle von polypeptidproduktion und -aufreinigung mit oberflächen verstärkter laserdesorption/ionisations massenspektrometrie

Info

Publication number
ATE419329T1
ATE419329T1 AT02792236T AT02792236T ATE419329T1 AT E419329 T1 ATE419329 T1 AT E419329T1 AT 02792236 T AT02792236 T AT 02792236T AT 02792236 T AT02792236 T AT 02792236T AT E419329 T1 ATE419329 T1 AT E419329T1
Authority
AT
Austria
Prior art keywords
laser desorption
purification
ionization mass
enhanced laser
mass spectrometry
Prior art date
Application number
AT02792236T
Other languages
English (en)
Inventor
Egisto Boschetti
Lisa Bradbury
Huw Davies
Lee Lomas
Thang Pham
Vanitha Thulasiraman
Tai-Tung Yip
Original Assignee
Biorad Lab Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biorad Lab Inc filed Critical Biorad Lab Inc
Application granted granted Critical
Publication of ATE419329T1 publication Critical patent/ATE419329T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/02—Details
    • H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
    • H01J49/0409—Sample holders or containers
    • H01J49/0418—Sample holders or containers for laser desorption, e.g. matrix-assisted laser desorption/ionisation [MALDI] plates or surface enhanced laser desorption/ionisation [SELDI] plates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/84—Preparation of the fraction to be distributed
    • G01N2030/8411—Intermediate storage of effluent, including condensation on surface
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Genetics & Genomics (AREA)
  • Urology & Nephrology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Optics & Photonics (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT02792236T 2001-11-14 2002-11-08 Verfahren zur kontrolle von polypeptidproduktion und -aufreinigung mit oberflächen verstärkter laserdesorption/ionisations massenspektrometrie ATE419329T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US33560901P 2001-11-14 2001-11-14
US10/124,626 US20030091976A1 (en) 2001-11-14 2002-04-16 Methods for monitoring polypeptide production and purification using surface enhanced laser desorption/ionization mass spectrometry

Publications (1)

Publication Number Publication Date
ATE419329T1 true ATE419329T1 (de) 2009-01-15

Family

ID=26822785

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02792236T ATE419329T1 (de) 2001-11-14 2002-11-08 Verfahren zur kontrolle von polypeptidproduktion und -aufreinigung mit oberflächen verstärkter laserdesorption/ionisations massenspektrometrie

Country Status (8)

Country Link
US (2) US20030091976A1 (de)
EP (1) EP1448760B1 (de)
JP (1) JP2005509173A (de)
AT (1) ATE419329T1 (de)
AU (1) AU2002357699A1 (de)
CA (1) CA2465214A1 (de)
DE (1) DE60230660D1 (de)
WO (1) WO2003042233A2 (de)

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DE60326864D1 (de) 2002-07-24 2009-05-07 Bio Rad Laboratories Proteininteraktionsdifferenzabbildung
WO2004061410A2 (en) * 2002-12-18 2004-07-22 Ciphergen Biosystems, Inc. Serum biomarkers in lung cancer
US20060205010A1 (en) * 2003-04-22 2006-09-14 Catherine Allioux Methods of host cell protein analysis
US7529629B2 (en) * 2003-04-28 2009-05-05 Cerno Bioscience Llc Computational methods and systems for multidimensional analysis
US20050164398A1 (en) * 2004-01-26 2005-07-28 Alexander James N.Iv Method for determining molecular weight of polymers
US7906630B2 (en) * 2004-04-27 2011-03-15 PerkinElmer Heath Sciences, Inc. Method for identifying peptides in a biological sample
JP5008564B2 (ja) * 2004-05-20 2012-08-22 ウオーターズ・テクノロジーズ・コーポレイシヨン 混合物中のタンパク質を同定する方法および装置
US20070142629A1 (en) * 2004-06-16 2007-06-21 Ciphergen Biosystems, Inc. Multichemistry fractionation
JP4659831B2 (ja) * 2005-07-29 2011-03-30 エーザイ・アール・アンド・ディー・マネジメント株式会社 化合物に対するタンパク質の結合力を解析する方法
JP2007108015A (ja) * 2005-10-13 2007-04-26 Biologica:Kk 分析用の保持体及びその利用
US20090181423A1 (en) 2007-12-31 2009-07-16 Baxter International Inc. Substantially animal protein-free recombinant furin and methods for producing same
EP2255188B1 (de) * 2008-02-29 2016-08-24 Imec Biosensor zum nachweis von neurotransmittern
JP5365116B2 (ja) * 2008-09-19 2013-12-11 横河電機株式会社 イオンクロマトグラフ測定装置およびイオンクロマトグラフ測定方法
WO2012031082A2 (en) * 2010-09-02 2012-03-08 University Of The Sciences In Philadelphia System and method for ionization of molecules for mass spectrometry and ion mobility spectrometry
US20140166875A1 (en) 2010-09-02 2014-06-19 Wayne State University Systems and methods for high throughput solvent assisted ionization inlet for mass spectrometry
US8853621B2 (en) 2010-10-25 2014-10-07 Wayne State University Systems and methods extending the laserspray ionization mass spectrometry concept from atmospheric pressure to vacuum
AU2011343813B2 (en) 2010-12-15 2015-05-21 Takeda Pharmaceutical Company Limited Eluate collection using conductivity gradient
US8506797B2 (en) * 2011-04-10 2013-08-13 Therapeutic Proteins International, LLC Downstream bioprocessing device
ES2834113T3 (es) 2013-03-15 2021-06-16 H Lundbeck As Purificación de anticuerpos y monitorización de la pureza
US9891203B2 (en) * 2013-06-05 2018-02-13 Dh Technologies Development Pte. Ltd. SWATH# data-independent acquisition technology for the detection of host cell protein contaminants in biotherapeutics protein products
TW202204596A (zh) * 2014-06-06 2022-02-01 美商健臻公司 灌注培養方法及其用途
IL290358B2 (en) * 2016-04-14 2024-03-01 Lonza Ag Preparations and methods for detecting host cell proteins
CN109118004B (zh) * 2018-08-16 2021-09-14 李宏伟 一种工程构筑选址适宜区预测方法
EP3867943A1 (de) * 2018-10-18 2021-08-25 DH Technologies Development Pte. Ltd. Funktionalisierung eines probennahmeelements zur verwendung mit einem massenspektrometriesystem
CN120303045A (zh) * 2022-11-26 2025-07-11 恩维达治疗公司 用于鉴定形成、稳定或破坏分子复合物的化合物的方法和系统
CN119086941B (zh) * 2024-11-05 2025-02-28 融致丰生制药有限公司 一种多肽提取物的质谱检测分析方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1347493A3 (de) * 1993-05-28 2005-11-23 Baylor College Of Medicine Verfahren und Gerät zur Desorption und Ionisierung von Analyte
US20020151707A1 (en) * 1995-06-07 2002-10-17 Corixa Corp. Immune mediators and related methods
DE69729101T2 (de) * 1996-06-07 2005-05-12 Immunivest Corp., Wilmington Magnetische trennung mit hilfe von externen und internen gradienten
EP0827966B1 (de) * 1996-09-09 2001-11-21 Rmf Dictagene S.A. Herstellung von gereinigten (Poly)Peptiden
NZ516848A (en) * 1997-06-20 2004-03-26 Ciphergen Biosystems Inc Retentate chromatography apparatus with applications in biology and medicine
US6207370B1 (en) * 1997-09-02 2001-03-27 Sequenom, Inc. Diagnostics based on mass spectrometric detection of translated target polypeptides
US6210910B1 (en) * 1998-03-02 2001-04-03 Trustees Of Tufts College Optical fiber biosensor array comprising cell populations confined to microcavities
ATE253126T1 (de) * 1998-08-25 2003-11-15 Univ Washington Schnelle quantitative analyse von proteinen oder proteinfunktionen in komplexen gemischen
US6558946B1 (en) * 2000-08-29 2003-05-06 The United States Of America As Represented By The Secretary Of The Army Automated sample processing for identification of microorganisms and proteins

Also Published As

Publication number Publication date
US20030091976A1 (en) 2003-05-15
WO2003042233A3 (en) 2004-01-08
DE60230660D1 (de) 2009-02-12
US20070059776A1 (en) 2007-03-15
AU2002357699A1 (en) 2003-05-26
EP1448760B1 (de) 2008-12-31
WO2003042233A2 (en) 2003-05-22
EP1448760A4 (de) 2005-01-05
EP1448760A2 (de) 2004-08-25
JP2005509173A (ja) 2005-04-07
CA2465214A1 (en) 2003-05-22

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