ATE516368T1 - Erstellung eines echtzeit-genexpressionsprofils - Google Patents

Erstellung eines echtzeit-genexpressionsprofils

Info

Publication number
ATE516368T1
ATE516368T1 AT04719806T AT04719806T ATE516368T1 AT E516368 T1 ATE516368 T1 AT E516368T1 AT 04719806 T AT04719806 T AT 04719806T AT 04719806 T AT04719806 T AT 04719806T AT E516368 T1 ATE516368 T1 AT E516368T1
Authority
AT
Austria
Prior art keywords
methods
monitoring
amplification
creation
real
Prior art date
Application number
AT04719806T
Other languages
English (en)
Inventor
Vladimir Slepnev
Original Assignee
Primeradx Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Primeradx Inc filed Critical Primeradx Inc
Application granted granted Critical
Publication of ATE516368T1 publication Critical patent/ATE516368T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT04719806T 2003-03-12 2004-03-11 Erstellung eines echtzeit-genexpressionsprofils ATE516368T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/387,286 US7081339B2 (en) 2002-04-12 2003-03-12 Methods for variation detection
PCT/US2004/007544 WO2004081228A2 (en) 2003-03-12 2004-03-11 Real time gene expression profiling

Publications (1)

Publication Number Publication Date
ATE516368T1 true ATE516368T1 (de) 2011-07-15

Family

ID=32987332

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04719806T ATE516368T1 (de) 2003-03-12 2004-03-11 Erstellung eines echtzeit-genexpressionsprofils

Country Status (5)

Country Link
US (4) US7081339B2 (de)
EP (1) EP1601780B1 (de)
JP (2) JP2006519611A (de)
AT (1) ATE516368T1 (de)
WO (1) WO2004081228A2 (de)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1587940A4 (de) 2002-12-20 2006-06-07 Caliper Life Sciences Inc Einzelmolekül amplifikation und dna-nachweis
US8275554B2 (en) * 2002-12-20 2012-09-25 Caliper Life Sciences, Inc. System for differentiating the lengths of nucleic acids of interest in a sample
US20050042639A1 (en) * 2002-12-20 2005-02-24 Caliper Life Sciences, Inc. Single molecule amplification and detection of DNA length
US20070154889A1 (en) * 2004-06-25 2007-07-05 Veridex, Llc Methods and reagents for the detection of melanoma
WO2006099255A2 (en) 2005-03-10 2006-09-21 Gen-Probe Incorporated Systems and methods to perform assays for detecting or quantifying analytes within samples
US7368246B2 (en) 2005-09-15 2008-05-06 Primera Biosystems, Inc. Quantitative gene expression profiling
JP4981295B2 (ja) * 2005-10-04 2012-07-18 キヤノン株式会社 Ｄｎａ処理方法およびｄｎａ処理装置
US7833716B2 (en) 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
US20080124716A1 (en) * 2006-11-29 2008-05-29 Northrop Grumman Systems Corporation Method and device for time-effective biomolecule detection
ES2453107T3 (es) 2007-06-11 2014-04-04 Wako Pure Chemical Industries, Ltd. Procedimiento de amplificación de ácidos nucleicos usando una PCR sobre microchip con detección integrada de CE en tiempo real
US20090197254A1 (en) * 2007-12-14 2009-08-06 Ming-Chou Lee Variant scorpion primers for nucleic acid amplification and detection
US8709726B2 (en) * 2008-03-11 2014-04-29 Sequenom, Inc. Nucleic acid-based tests for prenatal gender determination
US20110014723A1 (en) * 2008-03-14 2011-01-20 Erdman Dean D Compositions and processes relating to human bocavirus
US8962247B2 (en) 2008-09-16 2015-02-24 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses
US8476013B2 (en) 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
GB0901593D0 (en) 2009-01-30 2009-03-11 Touchlight Genetics Ltd Production of closed linear DNA
US8832581B2 (en) * 2009-03-05 2014-09-09 Ming Zhang Gene expression browser for web-based search and visualization of characteristics of gene expression
US9926593B2 (en) 2009-12-22 2018-03-27 Sequenom, Inc. Processes and kits for identifying aneuploidy
US9046507B2 (en) 2010-07-29 2015-06-02 Gen-Probe Incorporated Method, system and apparatus for incorporating capacitive proximity sensing in an automated fluid transfer procedure
GB201013153D0 (en) 2010-08-04 2010-09-22 Touchlight Genetics Ltd Primer for production of closed linear DNA
CN103403533B (zh) 2011-02-24 2017-02-15 简.探针公司 用于分辨光信号检测器中不同调制频率的光信号的系统和方法
CA2834218C (en) 2011-04-29 2021-02-16 Sequenom, Inc. Quantification of a minority nucleic acid species using inhibitory oligonucleotides
US8932989B2 (en) 2011-07-25 2015-01-13 Bioinventors & Entrepreneurs Network, Llc Sieving of nucleic acid samples
US9133567B2 (en) 2011-07-25 2015-09-15 Bioinventors & Entrepreneurs Network Llc Method for determining an attribute profile of biological samples
US9428799B2 (en) 2011-07-25 2016-08-30 Bioinventors & Entrepreneurs Network Llc Method for determining an allele profile of nucleic acid
WO2013124738A2 (en) 2012-02-21 2013-08-29 Oslo Universitetssykehus Hf Methods and biomarkers for detection and prognosis of cervical cancer
EP2820129A1 (de) 2012-03-02 2015-01-07 Sequenom, Inc. Verfahren und prozesse zur nichtinvasiven beurteilung genetischer variationen
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
US10504613B2 (en) 2012-12-20 2019-12-10 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
WO2014011928A1 (en) 2012-07-13 2014-01-16 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
EP2971100A1 (de) 2013-03-13 2016-01-20 Sequenom, Inc. Primer zur analyse der dna-methylierung
US20150127842A1 (en) * 2013-11-05 2015-05-07 D2 Technologies Inc. Method for Extending Application Interface for Future Applications
GB201320071D0 (en) * 2013-11-13 2013-12-25 Univ Leuven Kath Monitoring multiplex DNA amplification
EP3736344A1 (de) 2014-03-13 2020-11-11 Sequenom, Inc. Verfahren und prozesse zur nichtinvasiven beurteilung genetischer variationen
GB201415789D0 (en) 2014-09-05 2014-10-22 Touchlight Genetics Ltd Synthesis of DNA
JP7708408B2 (ja) * 2018-05-14 2025-07-15 国立大学法人神戸大学 二本鎖ｄｎａ合成方法
EP3739064A1 (de) 2019-05-15 2020-11-18 Biotype GmbH Vergleichende analyse von mikrosatelliten mittels kapillarelektrophorese (ce) -dna-profile
EP4074839A1 (de) 2021-04-16 2022-10-19 Biotype GmbH Optimierte oligonukleotid-tx-sonde für eine multiplexing-analyse von nukleinsäuren und ein multiplexing-verfahren
EP4350002A1 (de) 2022-10-07 2024-04-10 Biotype GmbH Détection d'analytes moléculaires basée sur la concurrence de sondes sur mesure
WO2025009019A1 (ja) * 2023-07-03 2025-01-09 株式会社日立ハイテク Ｄｎａ分析システム
DE102023124346A1 (de) 2023-09-09 2025-03-13 Biotype Gmbh Verfahren zur Detektion des Endometriumkarzinoms Subtyp CN hoch

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
DE69429038T2 (de) 1993-07-28 2002-03-21 Pe Corporation (Ny), Norwalk Vorrichtung und Verfahren zur Nukleinsäurevervielfältigung
US6479235B1 (en) 1994-09-30 2002-11-12 Promega Corporation Multiplex amplification of short tandem repeat loci
US5710628A (en) * 1994-12-12 1998-01-20 Visible Genetics Inc. Automated electrophoresis and fluorescence detection apparatus and method
ATE196322T1 (de) * 1995-05-05 2000-09-15 Perkin Elmer Corp Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay
JP4468488B2 (ja) * 1996-05-29 2010-05-26 コーネル・リサーチ・ファンデーション・インコーポレイテッド 組み合せたリガーゼ検出およびポリメラーゼ連鎖反応を用いる核酸配列相違の検出
JP4000605B2 (ja) * 1996-07-24 2007-10-31 株式会社日立製作所 Ｄｎａ試料調整装置及びこれを用いる電気泳動分析装置
US6228589B1 (en) 1996-10-11 2001-05-08 Lynx Therapeutics, Inc. Measurement of gene expression profiles in toxicity determination
CA2278465C (en) 1997-01-30 2010-04-27 University Technology Corporation Diagnosis and treatment of myocardial failure
US5994068A (en) * 1997-03-11 1999-11-30 Wisconsin Alumni Research Foundation Nucleic acid indexing
US6143496A (en) * 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
WO1999014368A2 (en) * 1997-09-15 1999-03-25 Whitehead Institute For Biomedical Research Methods and apparatus for processing a sample of biomolecular analyte using a microfabricated device
JP4217318B2 (ja) * 1998-12-07 2009-01-28 オリンパス株式会社 標的核酸の定量分析方法および装置
US7115365B2 (en) * 1998-12-07 2006-10-03 Olympus Optical Co., Ltd. Method of analyzing a target nucleic acid
US6303305B1 (en) * 1999-03-30 2001-10-16 Roche Diagnostics, Gmbh Method for quantification of an analyte
CA2644688C (en) * 1999-04-20 2012-11-13 Kankyo Engineering Co., Ltd. Method for determining a concentration of target nucleic acid molecules, nucleic acid probes for the method, and method for analysing data obtained by the method
JP2002543418A (ja) 1999-04-29 2002-12-17 ゲノム セラピューティックス コーポレーション 流体操作、サーモサイクリング、及び精製を一体化した迅速なｄｎａサンプル処理装置
US6440682B1 (en) * 1999-05-28 2002-08-27 Detroit R&D Inc. Detection of hypertension using immunoreactive metabolic products
US6221600B1 (en) * 1999-10-08 2001-04-24 Board Of Regents, The University Of Texas System Combinatorial oligonucleotide PCR: a method for rapid, global expression analysis
EP1257664A4 (de) * 2000-01-28 2006-04-05 Althea Technologies Inc Verfahren zur analyse der genexpression
CA2409752A1 (en) * 2000-05-16 2001-11-22 Children's Mercy Hospital Single copy genomic hybridization probes and method of generating same
US6531282B1 (en) 2000-05-30 2003-03-11 Oligotrail, Llc Multiplex amplification and analysis of selected STR loci
WO2002015949A2 (en) 2000-08-25 2002-02-28 Genome Therapeutics Corporation Device for identifying the presence of a nucleotide sequence in a dna sample
JP2004532615A (ja) * 2000-12-27 2004-10-28 インヴィトロジェン コーポレーション 核酸の検出および識別のためのプライマーおよび方法
US6482615B2 (en) * 2001-03-02 2002-11-19 Integrated Genetic Devices Ltd. Method and apparatus for effecting rapid thermal cycling of samples in microtiter plate size
CA2348042A1 (en) * 2001-06-04 2002-12-04 Ann Huletsky Sequences for detection and identification of methicillin-resistant staphylococcus aureus
US20030077611A1 (en) * 2001-10-24 2003-04-24 Sention Methods and systems for dynamic gene expression profiling
US7445893B2 (en) * 2002-04-12 2008-11-04 Primera Biosystems, Inc. Sampling method for amplification reaction analysis
KR20050075436A (ko) * 2002-11-21 2005-07-20 프리메라 바이오시스템스 증폭 반응 분석을 위한 샘플링 방법과 장치

Also Published As

Publication number Publication date
WO2004081228A2 (en) 2004-09-23
US20070037189A1 (en) 2007-02-15
EP1601780B1 (de) 2011-07-13
WO2004081228A3 (en) 2006-03-16
JP2013099332A (ja) 2013-05-23
US8182995B2 (en) 2012-05-22
US20130210013A1 (en) 2013-08-15
US20030235844A1 (en) 2003-12-25
EP1601780A2 (de) 2005-12-07
US7674582B2 (en) 2010-03-09
EP1601780A4 (de) 2008-05-21
JP2006519611A (ja) 2006-08-31
US20100221725A1 (en) 2010-09-02
JP5725670B2 (ja) 2015-05-27
US7081339B2 (en) 2006-07-25

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