ATE422214T1 - Reagenz-kit zur vorbereitung einer testlösung für eine nukleinsäureamplifizierungsreaktion und methode zur ermittlung von nukleinsäuren - Google Patents
Reagenz-kit zur vorbereitung einer testlösung für eine nukleinsäureamplifizierungsreaktion und methode zur ermittlung von nukleinsäurenInfo
- Publication number
- ATE422214T1 ATE422214T1 AT05026593T AT05026593T ATE422214T1 AT E422214 T1 ATE422214 T1 AT E422214T1 AT 05026593 T AT05026593 T AT 05026593T AT 05026593 T AT05026593 T AT 05026593T AT E422214 T1 ATE422214 T1 AT E422214T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acid
- amplification reaction
- preparing
- acid amplification
- test solution
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title abstract 6
- 102000039446 nucleic acids Human genes 0.000 title abstract 6
- 108020004707 nucleic acids Proteins 0.000 title abstract 6
- 230000003321 amplification Effects 0.000 title abstract 4
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 4
- 239000003153 chemical reaction reagent Substances 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- 239000012085 test solution Substances 0.000 title 1
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 abstract 2
- 239000012472 biological sample Substances 0.000 abstract 2
- 239000012488 sample solution Substances 0.000 abstract 2
- 239000003125 aqueous solvent Substances 0.000 abstract 1
- 239000003112 inhibitor Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6846—Common amplification features
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2004353849A JP4719455B2 (ja) | 2004-12-07 | 2004-12-07 | 直接核酸増幅方法用生体試料処理液および直接核酸増幅方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE422214T1 true ATE422214T1 (de) | 2009-02-15 |
Family
ID=35685830
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT05026593T ATE422214T1 (de) | 2004-12-07 | 2005-12-06 | Reagenz-kit zur vorbereitung einer testlösung für eine nukleinsäureamplifizierungsreaktion und methode zur ermittlung von nukleinsäuren |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US8465924B2 (de) |
| EP (1) | EP1669449B1 (de) |
| JP (1) | JP4719455B2 (de) |
| CN (1) | CN1978666B (de) |
| AT (1) | ATE422214T1 (de) |
| DE (1) | DE602005012619D1 (de) |
| ES (1) | ES2321627T3 (de) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4829509B2 (ja) * | 2005-02-18 | 2011-12-07 | シスメックス株式会社 | 遺伝子検査方法 |
| JP2008017832A (ja) * | 2006-06-13 | 2008-01-31 | Sysmex Corp | がんの転移の判定方法及び判定装置 |
| JP4943074B2 (ja) * | 2006-07-03 | 2012-05-30 | シスメックス株式会社 | がん転移の判定方法及び装置 |
| JP2008194028A (ja) * | 2007-01-15 | 2008-08-28 | Sysmex Corp | 胃がんのリンパ節転移判定方法 |
| JP5303132B2 (ja) * | 2007-09-20 | 2013-10-02 | シスメックス株式会社 | がん細胞の存否を判定する方法及び装置 |
| KR101935367B1 (ko) | 2012-07-26 | 2019-01-04 | 삼성전자주식회사 | 이온성 액체를 사용하여 생물학적 시료로부터 핵산 증폭의 증폭 효율 및 감도를 증가시키는 방법 |
| JP5951545B2 (ja) | 2013-03-29 | 2016-07-13 | シスメックス株式会社 | 検体分析装置、検体分析方法、及びコンピュータプログラム |
| JP2015087329A (ja) | 2013-10-31 | 2015-05-07 | シスメックス株式会社 | 吸引部の位置調整方法及び検体処理装置 |
| US9528105B2 (en) | 2014-09-04 | 2016-12-27 | Techlab, Inc. | Nucleic acid extraction using organic solvents to remove inhibitors |
| JP2016182112A (ja) * | 2015-03-25 | 2016-10-20 | 東ソー株式会社 | ウイルスからの核酸抽出試薬 |
| US20160289752A1 (en) * | 2015-03-31 | 2016-10-06 | Transponics | Method for the Isothermic Amplification of Nucleic Acid and SNP Detection |
| JP6679852B2 (ja) * | 2015-07-28 | 2020-04-15 | 東ソー株式会社 | 核酸の検出方法および当該方法を利用した試薬キット |
| KR102295681B1 (ko) * | 2016-05-19 | 2021-08-27 | 위스콘신 얼럼나이 리서어치 화운데이션 | 생물학적 분자의 용매 접근성 및 3차원 구조를 연구하기 위한 방법, 시스템, 및 조성물 |
| JP7555179B2 (ja) * | 2018-11-30 | 2024-09-24 | 株式会社島津製作所 | ノロウイルスの検出方法 |
| CN112921071A (zh) * | 2021-02-24 | 2021-06-08 | 上海基灵生物科技有限公司 | 一种猫体液样本处理试剂、检测试剂盒及应用 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3637655A (en) * | 1967-09-01 | 1972-01-25 | Hazleton Lab Inc | Method for extracting water-soluble substances from biological materials |
| US3673655A (en) | 1970-05-18 | 1972-07-04 | Renault | Milling cutters with detachable blades |
| US5521061A (en) * | 1992-07-17 | 1996-05-28 | Aprogenex, Inc. | Enhancement of probe signal in nucleic acid-mediated in-situ hybridization studies |
| AU4934099A (en) | 1998-08-04 | 2000-02-28 | Center For Advanced Science And Technology Incubation, Ltd. | Method for enzymatic amplification of nucleic acid |
| CA2285729A1 (en) * | 1999-10-08 | 2001-04-08 | Donald L. Smith | Method of increasing photosynthesis in plants comprising an exposure thereof to salicylic acid and compositions therefor |
| ATE427992T1 (de) * | 2001-11-14 | 2009-04-15 | Toyo Boseki | Dna-synthesepromotoren, mit dna-polymerase assoziierte faktoren und nutzung davon |
| WO2003060116A1 (en) | 2002-01-09 | 2003-07-24 | Sysmex Corporation | Nucleic acid detection method and system thereof |
| US7517697B2 (en) * | 2003-02-05 | 2009-04-14 | Applied Biosystems, Llc | Compositions and methods for preserving RNA in biological samples |
-
2004
- 2004-12-07 JP JP2004353849A patent/JP4719455B2/ja not_active Expired - Fee Related
-
2005
- 2005-12-06 AT AT05026593T patent/ATE422214T1/de not_active IP Right Cessation
- 2005-12-06 CN CN2005101287692A patent/CN1978666B/zh not_active Expired - Fee Related
- 2005-12-06 US US11/294,545 patent/US8465924B2/en not_active Expired - Fee Related
- 2005-12-06 DE DE602005012619T patent/DE602005012619D1/de not_active Expired - Lifetime
- 2005-12-06 EP EP05026593A patent/EP1669449B1/de not_active Expired - Lifetime
- 2005-12-06 ES ES05026593T patent/ES2321627T3/es not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JP2006158283A (ja) | 2006-06-22 |
| CN1978666A (zh) | 2007-06-13 |
| DE602005012619D1 (de) | 2009-03-19 |
| ES2321627T3 (es) | 2009-06-09 |
| JP4719455B2 (ja) | 2011-07-06 |
| US20060121515A1 (en) | 2006-06-08 |
| EP1669449B1 (de) | 2009-02-04 |
| US8465924B2 (en) | 2013-06-18 |
| CN1978666B (zh) | 2012-05-30 |
| EP1669449A1 (de) | 2006-06-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ATE422214T1 (de) | Reagenz-kit zur vorbereitung einer testlösung für eine nukleinsäureamplifizierungsreaktion und methode zur ermittlung von nukleinsäuren | |
| ATE450623T1 (de) | Modifikatoren der lichtemission und ihre verwendungen zur detektion, amplifikation und analyse von nukleinsäuren | |
| ATE384803T1 (de) | Verfahren zur schnellen bestimmung von mikrobieller dns/rns, kit dafür und verwendung des verfahrens | |
| DE69016079D1 (de) | Methode zur Extraktion, Amplifikation und zum Nachweis einer Nukleinsäure aus einer Fraktion mononuklearer Zellen von peripherem Blut. | |
| ATE373729T1 (de) | Verfahren zur selektiven detektion einer zielnukleinsäure | |
| ATE397092T1 (de) | Methode zur direkten sequenzierung von nukleinsäuren | |
| ATE114334T1 (de) | Chaotropisches verfahren zur schätzung von nukleinen säuren in einer biologischen probe. | |
| DE60105411D1 (de) | Verfahren zur diagnose von krebs | |
| DE602004031152D1 (de) | Verfahren und system zum analysieren von reaktione | |
| ATE430812T1 (de) | Materialien und verfahren zum einfangen von krankheitserregern und zur abtrennung von aurintricarbonsäure aus einer probe | |
| ATE493508T1 (de) | Verfahren, kits und vorrichtungen zur prozessierung von nukleinsäuren | |
| DE60041489D1 (de) | Nachweis von hiv-1 durch amplifizierung von nukleinsäuren | |
| WO2009097511A3 (en) | Two stage enrichment of cell-free fetal dna in maternal plasma | |
| ATE286984T1 (de) | Methode und testsatz zur detektion von schistosomiasis mittels polymerasekettenreaktion | |
| ATE372392T1 (de) | Verfahren zur bestimmung der menge einer zielnukleinsäure in einer probe | |
| ATE519116T1 (de) | Verfahren zur normierung der konzentration von analyten in einer urinprobe | |
| DE60334604D1 (de) | ||
| DE69834506D1 (de) | Fluorometrisches verfahren zur überwachung und zum nachweis von nucleinsäure-amplifizierung | |
| ATE493494T1 (de) | Probenherstellungsverfahren mit einem alkali- stoss | |
| DE60142294D1 (de) | Verfahren zum vorbehandeln einer probe | |
| CN109207615B (zh) | 一种免标记荧光检测金黄色葡萄球菌mecA基因的方法及检测试剂盒 | |
| ATE537191T1 (de) | Protein, verfahren zur proteinimmobilisierung, struktur, biosensor, nukleinsäure, vektor und kit für den nachweis einer zielsubstanz | |
| ATE401572T1 (de) | Reagens und verfahren zum nachweis von verfälschungsmitteln in wässrigen proben | |
| CN112921120A (zh) | 一种可重复利用的核酸荧光探针及相应的核酸检测方法 | |
| DE602005027809D1 (de) | Primer und sonden zum nachweis von hiv |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |