ATE426611T1 - Verfahren zur reinigung eines kalziumionen- bindenden proteins - Google Patents

Verfahren zur reinigung eines kalziumionen- bindenden proteins

Info

Publication number
ATE426611T1
ATE426611T1 AT01951897T AT01951897T ATE426611T1 AT E426611 T1 ATE426611 T1 AT E426611T1 AT 01951897 T AT01951897 T AT 01951897T AT 01951897 T AT01951897 T AT 01951897T AT E426611 T1 ATE426611 T1 AT E426611T1
Authority
AT
Austria
Prior art keywords
calcium ion
binding protein
purifying
protein
present
Prior art date
Application number
AT01951897T
Other languages
English (en)
Inventor
Hiroshi Mizokami
Shinichi Furukawa
Keishin Sugawara
Tatsufumi Onchi
Kazuhiro Komatsu
Satoshi Koyanagi
Hideo Yoshizaki
Original Assignee
Chemo Sero Therapeut Res Inst
Kowa Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chemo Sero Therapeut Res Inst, Kowa Co filed Critical Chemo Sero Therapeut Res Inst
Application granted granted Critical
Publication of ATE426611T1 publication Critical patent/ATE426611T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6432—Coagulation factor Xa (3.4.21.6)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4721—Lipocortins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4728—Calcium binding proteins, e.g. calmodulin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21006—Coagulation factor Xa (3.4.21.6)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Removal Of Specific Substances (AREA)
AT01951897T 2000-07-21 2001-07-18 Verfahren zur reinigung eines kalziumionen- bindenden proteins ATE426611T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2000220600 2000-07-21

Publications (1)

Publication Number Publication Date
ATE426611T1 true ATE426611T1 (de) 2009-04-15

Family

ID=18715177

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01951897T ATE426611T1 (de) 2000-07-21 2001-07-18 Verfahren zur reinigung eines kalziumionen- bindenden proteins

Country Status (9)

Country Link
US (2) US6869934B2 (de)
EP (1) EP1306383B1 (de)
KR (1) KR100841599B1 (de)
CN (1) CN1250567C (de)
AT (1) ATE426611T1 (de)
AU (1) AU2001272736A1 (de)
CA (1) CA2385371A1 (de)
DE (1) DE60138111D1 (de)
WO (1) WO2002008249A1 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5883849B2 (ja) 2010-04-29 2016-03-15 バクスター・インターナショナル・インコーポレイテッドＢａｘｔｅｒ Ｉｎｔｅｒｎａｔｉｏｎａｌ Ｉｎｃｏｒｐ０Ｒａｔｅｄ 陰イオン交換樹脂を用いる二価カチオン結合タンパク質の精製方法
CN102382168A (zh) * 2010-09-03 2012-03-21 中国科学院过程工程研究所 一种高效纯化金属离子结合蛋白的离子交换层析分离纯化方法
LT2748179T (lt) 2011-10-14 2022-04-11 Takeda Pharmaceutical Company Limited Baltymų gryninimas anijonų mainų chromatografija
EP2748180B1 (de) * 2011-10-14 2018-08-15 Baxalta GmbH Proteinaufreinigung mithilfe der anionenaustauschchromatographie
TR201808584T4 (tr) 2013-03-15 2018-07-23 Baxalta GmbH Anyon değişimi kromotografisi aracılığıyla k vitaminine bağlı proteinler için saflaştırma yöntemi.
CN109562352B (zh) * 2016-08-01 2021-12-28 株式会社钟化 钙蛋白粒子的吸附材料以及吸附除去系统及其利用方法
EP3862078A1 (de) * 2020-02-05 2021-08-11 Pentracor GmbH Verwendung einer alkalihydroxid-lösung zur regeneration einer apheresesäule

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1265446A (en) 1985-09-30 1990-02-06 Masahiro Maki Anticoagulating substance, process for preparing same and anticoagulant comprising same as an effective component
JP2660514B2 (ja) 1987-02-20 1997-10-08 興和株式会社 抗血液凝固作用を有するポリペプチド
US4981952A (en) 1988-10-04 1991-01-01 Eli Lilly And Company Method for the purification of vitamin K-dependent proteins
EP0409053B1 (de) * 1989-07-15 1993-09-29 BOEHRINGER INGELHEIM INTERNATIONAL GmbH Verfahren zur Reinigung von Annexinen
JP2743207B2 (ja) 1990-01-25 1998-04-22 財団法人化学及血清療法研究所 Ｃｐｂ―ｉの製造方法並びにこれに用いる組換えプラスミドおよび形質転換酵母
DE4406515C1 (de) 1994-02-28 1995-10-19 Immuno Ag Verfahren zur Isolierung und Reinigung Vitamin K-abhängiger Proteine
WO1996003655A1 (en) * 1994-07-23 1996-02-08 Boehringer Mannheim Gmbh Method for the determination of the prethrombotic state
EP0731166A3 (de) 1995-03-06 1997-12-29 Tonen Corporation Neue Calcium-bindende Proteine
US5976832A (en) * 1995-03-06 1999-11-02 Tonen Corporation DNA encoding novel calcium-binding proteins
US5658877A (en) * 1995-05-18 1997-08-19 Wisconsin Alumni Research Foundation Method to treat endotoxin effects by administration of 33 kilodalton phospholipid binding protein
US6645465B2 (en) * 1999-08-06 2003-11-11 Michigan, University Of The Regents Annexin proteins and autoantibodies as serum markers for cancer

Also Published As

Publication number Publication date
EP1306383A1 (de) 2003-05-02
US6869934B2 (en) 2005-03-22
EP1306383B1 (de) 2009-03-25
KR20020057960A (ko) 2002-07-12
DE60138111D1 (de) 2009-05-07
US20020169294A1 (en) 2002-11-14
CA2385371A1 (en) 2002-01-31
EP1306383A4 (de) 2005-01-26
CN1392878A (zh) 2003-01-22
KR100841599B1 (ko) 2008-06-26
US20050059805A1 (en) 2005-03-17
CN1250567C (zh) 2006-04-12
AU2001272736A1 (en) 2002-02-05
WO2002008249A1 (en) 2002-01-31

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