LT2748179T - Baltymų gryninimas anijonų mainų chromatografija - Google Patents

Baltymų gryninimas anijonų mainų chromatografija

Info

Publication number
LT2748179T
LT2748179T LTEPPCT/EP2012/070257T LT12070257T LT2748179T LT 2748179 T LT2748179 T LT 2748179T LT 12070257 T LT12070257 T LT 12070257T LT 2748179 T LT2748179 T LT 2748179T
Authority
LT
Lithuania
Prior art keywords
anion exchange
exchange chromatography
protein purification
purification
protein
Prior art date
Application number
LTEPPCT/EP2012/070257T
Other languages
English (en)
Inventor
Artur Mitterer
Meinhard Hasslacher
Christian Fiedler
Original Assignee
Takeda Pharmaceutical Company Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Takeda Pharmaceutical Company Limited filed Critical Takeda Pharmaceutical Company Limited
Publication of LT2748179T publication Critical patent/LT2748179T/lt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/644—Coagulation factor IXa (3.4.21.22)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21022—Coagulation factor IXa (3.4.21.22)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Diabetes (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
LTEPPCT/EP2012/070257T 2011-10-14 2012-10-12 Baltymų gryninimas anijonų mainų chromatografija LT2748179T (lt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161547579P 2011-10-14 2011-10-14
PCT/EP2012/070257 WO2013053887A1 (en) 2011-10-14 2012-10-12 Protein purification by anion exchange chromatography

Publications (1)

Publication Number Publication Date
LT2748179T true LT2748179T (lt) 2022-04-11

Family

ID=47010608

Family Applications (1)

Application Number Title Priority Date Filing Date
LTEPPCT/EP2012/070257T LT2748179T (lt) 2011-10-14 2012-10-12 Baltymų gryninimas anijonų mainų chromatografija

Country Status (8)

Country Link
US (1) US10378004B2 (lt)
EP (1) EP2748179B1 (lt)
JP (2) JP6236008B2 (lt)
AU (1) AU2012322948B2 (lt)
ES (1) ES2908920T3 (lt)
LT (1) LT2748179T (lt)
PL (1) PL2748179T3 (lt)
WO (1) WO2013053887A1 (lt)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG185005A1 (en) 2010-04-29 2012-11-29 Baxter Int Purification method for divalent cation binding proteins on anion exchange resin
AU2012322948B2 (en) 2011-10-14 2014-11-06 Takeda Pharmaceutical Company Limited Protein purification by anion exchange chromatography
ES2700933T3 (es) 2011-10-14 2019-02-20 Baxalta GmbH Purificación de proteínas mediante cromatografía de intercambio aniónico
NZ629845A (en) 2013-03-15 2016-10-28 Baxalta Inc Purification method for vitamin k dependent proteins by anion exchange chromatography
AR102331A1 (es) * 2014-08-12 2017-02-22 Baxalta Inc Activación del factor x
EP3560946B1 (en) 2016-12-22 2022-02-02 KM Biologics Co., Ltd. Chromatographic method for collecting blood coagulation factor vii with high yield
EP4093755A4 (en) * 2020-01-24 2024-07-31 Alkermes Pharma Ireland Limited PURIFICATION PROCESSES
JP2022023813A (ja) * 2020-07-27 2022-02-08 ファイザー・インク 組換え生産されたｒｓｖタンパク質の精製方法における陰イオン交換クロマトグラフィー用洗浄溶液の改良
WO2022172284A1 (en) * 2021-02-11 2022-08-18 Gennova Biopharmaceuticals Ltd. Purification of a therapeutic protein
IL322843A (en) * 2023-03-14 2025-10-01 Amgen Inc Anion exchange chromatography processes using a primary amine ligand

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0317376B2 (fr) 1987-10-23 1996-04-03 Centre Regional De Transfusion Sanguine De Lille Préparation de concentré de facteur IX humain de haute pureté et d'autres protéines plasmatiques
US4981952A (en) 1988-10-04 1991-01-01 Eli Lilly And Company Method for the purification of vitamin K-dependent proteins
CA2143125C (en) * 1992-08-27 2008-09-23 Koenraad Mertens Antibodies specific for a haemostatic protein, their use for isolating intact protein, haemostatic compositions devoid of proteolytic cleavage products of the protein
DE4406515C1 (de) * 1994-02-28 1995-10-19 Immuno Ag Verfahren zur Isolierung und Reinigung Vitamin K-abhängiger Proteine
US5714583A (en) * 1995-06-07 1998-02-03 Genetics Institute, Inc. Factor IX purification methods
US6344596B1 (en) 1997-02-14 2002-02-05 American Red Cross Expression of active human factor IX in mammary tissue and of milk non human transgenic mammals
KR100841599B1 (ko) 2000-07-21 2008-06-26 자이단호진 가가쿠오요비겟세이료호겐쿠쇼 칼슘 이온-결합 단백질의 정제 방법
US20040106779A1 (en) 2002-12-03 2004-06-03 Bigler Douglas E. Modified factor IX preparation
US20090043080A1 (en) 2004-09-29 2009-02-12 Novo Nordisk Healthcare A/G Purification of a Bulk of a Factor VII Polypeptide by Fractionated Elution from an Anion-Exchange Material
EP2360171A1 (en) * 2004-12-23 2011-08-24 Novo Nordisk Health Care AG Reduction of the content of protein contaminants in compositions comprising a vitamin K-dependent protein of interest
EP1924688A1 (en) 2005-09-01 2008-05-28 Novo Nordisk Health Care AG Purification of coagulation factor vii polypeptides
WO2007101681A1 (en) 2006-03-07 2007-09-13 Baxter International Inc Highly phosphorylated and sulfated recombinant factor ix
FR2901796A1 (fr) 2006-05-31 2007-12-07 Lab Francais Du Fractionnement Procede d'extraction d'une ou de plusieurs proteines presentes dans du lait
FR2901707B1 (fr) 2006-05-31 2017-09-29 Lab Francais Du Fractionnement Composition de facteur vii recombinant ou transgenique, chaque molecule de facteur vii possedant deux sites de n-glycosylation a motifs glycanniques definis
DK2125866T3 (da) * 2007-02-28 2013-07-29 Baxter Int Fremgangsmåde til rensning af rekombinant blodkoagulationsfaktor IX, som er beriget med sulfaterede og/eller phosphorylerede molekyler
WO2011073235A1 (en) 2009-12-18 2011-06-23 Csl Ltd. Method of purifying polypeptides
SG185005A1 (en) 2010-04-29 2012-11-29 Baxter Int Purification method for divalent cation binding proteins on anion exchange resin
ES2700933T3 (es) * 2011-10-14 2019-02-20 Baxalta GmbH Purificación de proteínas mediante cromatografía de intercambio aniónico
AU2012322948B2 (en) 2011-10-14 2014-11-06 Takeda Pharmaceutical Company Limited Protein purification by anion exchange chromatography

Also Published As

Publication number Publication date
WO2013053887A1 (en) 2013-04-18
AU2012322948A1 (en) 2013-05-16
EP2748179B1 (en) 2021-12-29
EP2748179A1 (en) 2014-07-02
JP2017160248A (ja) 2017-09-14
JP6236008B2 (ja) 2017-11-22
US20140302591A1 (en) 2014-10-09
JP2014530230A (ja) 2014-11-17
JP6820232B2 (ja) 2021-01-27
AU2012322948B2 (en) 2014-11-06
PL2748179T3 (pl) 2022-04-11
ES2908920T3 (es) 2022-05-04
US10378004B2 (en) 2019-08-13
HK1198588A1 (en) 2015-04-30

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