ATE427954T1 - Abtrennung von lipopolysacchariden aus lipopolysaccharidkomplexen mit hilfe von nicht- entzundbaren lísungsmitteln - Google Patents

Abtrennung von lipopolysacchariden aus lipopolysaccharidkomplexen mit hilfe von nicht- entzundbaren lísungsmitteln

Info

Publication number
ATE427954T1
ATE427954T1 AT04755271T AT04755271T ATE427954T1 AT E427954 T1 ATE427954 T1 AT E427954T1 AT 04755271 T AT04755271 T AT 04755271T AT 04755271 T AT04755271 T AT 04755271T AT E427954 T1 ATE427954 T1 AT E427954T1
Authority
AT
Austria
Prior art keywords
lps
protein
alkanediols
complexes
separation
Prior art date
Application number
AT04755271T
Other languages
English (en)
Inventor
Philip Ropp
Michael Murray
Original Assignee
Organon Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Organon Nv filed Critical Organon Nv
Application granted granted Critical
Publication of ATE427954T1 publication Critical patent/ATE427954T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
AT04755271T 2003-06-24 2004-06-15 Abtrennung von lipopolysacchariden aus lipopolysaccharidkomplexen mit hilfe von nicht- entzundbaren lísungsmitteln ATE427954T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US48089903P 2003-06-24 2003-06-24
US48859603P 2003-07-18 2003-07-18

Publications (1)

Publication Number Publication Date
ATE427954T1 true ATE427954T1 (de) 2009-04-15

Family

ID=33567625

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04755271T ATE427954T1 (de) 2003-06-24 2004-06-15 Abtrennung von lipopolysacchariden aus lipopolysaccharidkomplexen mit hilfe von nicht- entzundbaren lísungsmitteln

Country Status (8)

Country Link
US (3) US20060189790A1 (de)
EP (1) EP1638990B1 (de)
JP (1) JP2007526225A (de)
AT (1) ATE427954T1 (de)
CA (1) CA2530454A1 (de)
DE (1) DE602004020473D1 (de)
ES (1) ES2323642T3 (de)
WO (1) WO2005003152A1 (de)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6399570B1 (en) * 1999-02-05 2002-06-04 Agennix, Inc. Antimicrobial/endotoxin neutralizing polypeptide
DE602004020473D1 (de) * 2003-06-24 2009-05-20 Organon Nv Abtrennung von lipopolysacchariden aus lipopolysaccharidkomplexen mit hilfe von nicht-entzündbaren lösungsmitteln
EP2409995A3 (de) 2007-02-28 2012-08-15 Lipoxen Technologies Limited Reduktion von Endotoxin aus Polysialinsäuren
CA2696145C (en) * 2007-07-10 2016-12-20 Glanbia Nutritionals Method for removing endotoxin from proteins
AU2014201943B2 (en) * 2007-07-10 2016-07-28 Glanbia Nutritionals Method for removing endotoxin from proteins
JP2012516878A (ja) * 2009-02-06 2012-07-26 ノボザイムス バイオファーマ デーコー アクティーゼルスカブ 精製プロセス
EP2421894A4 (de) 2009-04-24 2013-07-24 Westland Co Operative Dairy Company Ltd Verfahren zur herstellung von lactoferrin mit geringem eisengehalt
CN103880947B (zh) * 2012-12-21 2016-01-13 武汉禾元生物科技股份有限公司 一种分离纯化高纯度重组人血清白蛋白的层析方法
SI3155424T1 (en) 2014-06-12 2018-04-30 Hyglos Invest Gmbh Demonstration of endotoxins in the solution
EP2955518B2 (de) 2014-06-12 2022-01-26 bioMérieux Deutschland GmbH Demaskierung von Endotoxinen in Lösung
BR112017005850A2 (pt) 2014-09-24 2018-02-06 Basf Se processo e aparelho para preparação de um diéster tereftálico.
CN106715382A (zh) 2014-09-24 2017-05-24 巴斯夫欧洲公司 通过再循环醇脱水而制备对苯二甲酸二酯的方法
RU2668964C1 (ru) 2014-09-24 2018-10-05 Басф Се Способ получения сложных диэфиров терефталевой кислоты с перекачиванием реакционной смеси
EP3197861B1 (de) 2014-09-24 2018-08-01 Basf Se Verfahren zur herstellung von terephthalsäurediestern
CN105251049B (zh) * 2015-09-29 2018-04-10 浙江星月生物科技股份有限公司 一种去除生物医用材料中细菌内毒素的离子缓冲液及应用
DE102016106271B4 (de) 2016-04-06 2018-03-29 Axagarius Gmbh & Co. Kg Aufreinigung von Nukleinsäure aus einer Nukleinsäure und Endotoxin enthaltenden Probe
JP7033083B2 (ja) 2016-05-26 2022-03-09 サイトソーベンツ・コーポレーション 内毒素血症誘発分子を除去するための血液適合性多孔質ポリマービーズ収着材の使用

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2961293D1 (en) * 1978-02-17 1982-01-14 Nat Res Dev Immunogenic cell envelope preparations
US4832751A (en) * 1987-07-01 1989-05-23 The United States Of America As Represented By The Secretary Of Agriculture Method for reduction of endotoxin in cotton lint or dust
JP2577237B2 (ja) * 1988-02-05 1997-01-29 雪印乳業株式会社 樹脂担体からパイロジェンを除去する方法
DE69012682T2 (de) * 1989-06-12 1995-04-06 Merck & Co Inc Verfahren zur Entfernung von bakteriellen Endotoxinen aus gram-negativen Polysacchariden.
US5045456A (en) * 1989-06-12 1991-09-03 Merck & Co., Inc. Process for removing bacterial endotoxin from gram-negative polysaccharides
JP4113580B2 (ja) * 1994-02-07 2008-07-09 キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング エンドトキシンを減量又は除去する方法
US6265542B1 (en) * 1997-10-24 2001-07-24 Genentech, Inc. Purification of molecules
AU4314099A (en) * 1998-06-01 1999-12-20 Immune Response Corporation, The Novel method of large scale plasmid purification
DE10010342A1 (de) * 2000-03-06 2001-09-20 Merck Patent Gmbh Verfahren zur Abreicherung von Endotoxinen
US6966992B2 (en) * 2001-03-19 2005-11-22 Akzo Nobel Nv Method of purification of molecules using unbranched terminal alkyldiols
DE602004020473D1 (de) * 2003-06-24 2009-05-20 Organon Nv Abtrennung von lipopolysacchariden aus lipopolysaccharidkomplexen mit hilfe von nicht-entzündbaren lösungsmitteln

Also Published As

Publication number Publication date
WO2005003152A1 (en) 2005-01-13
EP1638990A1 (de) 2006-03-29
EP1638990B1 (de) 2009-04-08
US20110003976A1 (en) 2011-01-06
CA2530454A1 (en) 2005-01-13
DE602004020473D1 (de) 2009-05-20
US20130267691A1 (en) 2013-10-10
JP2007526225A (ja) 2007-09-13
US20060189790A1 (en) 2006-08-24
ES2323642T3 (es) 2009-07-22
WO2005003152A8 (en) 2005-03-10
US8399633B2 (en) 2013-03-19

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