ATE430195T1 - Verfahren und zusammensetzungen zur reinigung von nukleinsäure aus einer wirtszelle - Google Patents

Verfahren und zusammensetzungen zur reinigung von nukleinsäure aus einer wirtszelle

Info

Publication number
ATE430195T1
ATE430195T1 AT03816282T AT03816282T ATE430195T1 AT E430195 T1 ATE430195 T1 AT E430195T1 AT 03816282 T AT03816282 T AT 03816282T AT 03816282 T AT03816282 T AT 03816282T AT E430195 T1 ATE430195 T1 AT E430195T1
Authority
AT
Austria
Prior art keywords
nucleic acid
compositions
cells
host cell
methods
Prior art date
Application number
AT03816282T
Other languages
English (en)
Inventor
Michael Domanico
Matt Meyers
Kristian Keane
Lisa Braun
Thomas Kolzau
Original Assignee
Qiagen North American Holdings
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen North American Holdings filed Critical Qiagen North American Holdings
Application granted granted Critical
Publication of ATE430195T1 publication Critical patent/ATE430195T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/107497—Preparation composition [e.g., lysing or precipitation, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25125—Digestion or removing interfering materials
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/25375—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
    • Y10T436/255—Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.] including use of a solid sorbent, semipermeable membrane, or liquid extraction

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Detergent Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
AT03816282T 2003-03-12 2003-12-16 Verfahren und zusammensetzungen zur reinigung von nukleinsäure aus einer wirtszelle ATE430195T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/387,646 US20040180445A1 (en) 2003-03-12 2003-03-12 Methods and compositions for purification of nucleic acid from a host cell
PCT/US2003/040175 WO2004081175A2 (en) 2003-03-12 2003-12-16 Methods and compositions for purification of nucleic acid from a host cell

Publications (1)

Publication Number Publication Date
ATE430195T1 true ATE430195T1 (de) 2009-05-15

Family

ID=32961928

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03816282T ATE430195T1 (de) 2003-03-12 2003-12-16 Verfahren und zusammensetzungen zur reinigung von nukleinsäure aus einer wirtszelle

Country Status (7)

Country Link
US (2) US20040180445A1 (de)
EP (1) EP1601966B1 (de)
CN (1) CN1768265A (de)
AT (1) ATE430195T1 (de)
AU (1) AU2003297244A1 (de)
DE (1) DE60327471D1 (de)
WO (1) WO2004081175A2 (de)

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US20050009036A1 (en) * 2003-07-11 2005-01-13 Applera Corporation Methods and kits for obtaining nucleic acid from biological samples
EP1706502A4 (de) * 2003-11-26 2007-05-23 Eppendorf Ag Verfahren und zusammensetzungen zur in-vitro-amplifikation extrachromosomaler nukleinsäure
WO2006026248A1 (en) * 2004-08-25 2006-03-09 Sigma-Aldrich Co. Compositions and methods employing zwitterionic detergent combinations
EP1846579A4 (de) * 2005-01-21 2008-12-24 New York Blood Ct Inc Verfahren zur extraktion und identifikation von nukleinsäuren
US20060281142A1 (en) * 2005-04-19 2006-12-14 E.I. Du Pont De Nemours And Company Combined sample enrichment and disruption
KR100622606B1 (ko) 2005-06-16 2006-09-11 (주)엘피스바이오텍 단일공정에 의한 플라스미드 ｄｎａ 정제용 조성물과 그를이용하여 플라스미드 ｄｎａ를 정제하는 방법
JP2007006799A (ja) * 2005-06-30 2007-01-18 Tokyo Institute Of Technology プラスミドｄｎａの精製方法及びプラスミドｄｎａ精製用キット
WO2007070381A2 (en) 2005-12-09 2007-06-21 Promega Corporation Nucleic acid purification with a binding matrix
WO2007103485A2 (en) * 2006-03-08 2007-09-13 Promega Corporation Small rna purification
US20080003575A1 (en) * 2006-06-28 2008-01-03 Sigma-Aldrich Co. Methods and composition for RNA extraction
EP2484781B1 (de) * 2006-08-01 2017-08-23 Applied Biosystems, LLC Nachweis von Analyten und Nukleinsäuren
ATE490320T1 (de) * 2006-12-13 2010-12-15 Hoffmann La Roche Verwendung von acetalen zur isolation von nukleinsäuren
US20080146789A1 (en) * 2007-03-20 2008-06-19 Braman Jeffrey C Methods for the separation of biological molecules using dioxolane
CA2629589C (en) * 2007-04-20 2016-03-29 F.Hoffmann-La Roche Ag Isolation and purification of nucleic acid molecules with a solid phase
WO2009129524A2 (en) * 2008-04-18 2009-10-22 The General Hospital Corporation High-throughput plasmid dna preparation
US8039613B2 (en) 2009-08-28 2011-10-18 Promega Corporation Methods of purifying a nucleic acid and formulation and kit for use in performing such methods
US8222397B2 (en) 2009-08-28 2012-07-17 Promega Corporation Methods of optimal purification of nucleic acids and kit for use in performing such methods
US9725754B2 (en) * 2010-07-29 2017-08-08 Sean F. Boyle Generic sample preparation
DE112011102524T5 (de) * 2010-07-29 2013-07-11 Roche Diagnostics Gmbh Präparation generischer Proben
KR20130117760A (ko) * 2010-08-30 2013-10-28 에프. 호프만-라 로슈 아게 테트라넥틴-아포지단백질 a-i 입자의 제조 방법, 이에 의해 제조된 지질 입자 및 이의 용도
BR112013033562A2 (pt) 2011-08-25 2017-02-14 Hoffmann La Roche proteína de fusão de tetranectina-apolipoproteína a-i encurtada, uma partícula de lipídio que a contém e uso das mesmas
EP2647709B1 (de) 2012-04-05 2016-02-24 F. Hoffmann-La Roche AG Aminverbindungen für die selektive Herstellung von biologischen Proben
WO2014018195A1 (en) 2012-06-21 2014-01-30 Monsanto Technology Llc Lysis buffer and methods for extraction of dna from plant material
CN107254466A (zh) * 2017-07-01 2017-10-17 济凡生物科技（北京）有限公司 一种一步法提取质粒dna的试剂盒和方法
CN113186185B (zh) * 2020-01-14 2023-05-26 东北林业大学 一种从哺乳动物粪便中高效富集宿主dna的方法
CN114164113A (zh) * 2020-09-10 2022-03-11 西比曼生物科技（香港）有限公司 一种封闭式的菌体裂解工艺
CN112626174B (zh) * 2020-12-16 2024-05-07 广州源井生物科技有限公司 一种细胞裂解液及其应用
WO2024006548A1 (en) * 2022-07-01 2024-01-04 Sherlock Biosciences, Inc. Nucleic acid preparation

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Also Published As

Publication number Publication date
AU2003297244A8 (en) 2004-09-30
US20090246859A1 (en) 2009-10-01
WO2004081175A3 (en) 2005-02-03
US20040180445A1 (en) 2004-09-16
AU2003297244A1 (en) 2004-09-30
EP1601966B1 (de) 2009-04-29
EP1601966A4 (de) 2007-05-02
EP1601966A2 (de) 2005-12-07
DE60327471D1 (de) 2009-06-10
CN1768265A (zh) 2006-05-03
WO2004081175A2 (en) 2004-09-23
US7863050B2 (en) 2011-01-04

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