BRPI0606363A2 - método para o isolamento e/ou a purificação de ácidos nucleicos ácido nucleico, e, método para a imobilização de ácidos nucleicos em uma matriz - Google Patents
método para o isolamento e/ou a purificação de ácidos nucleicos ácido nucleico, e, método para a imobilização de ácidos nucleicos em uma matrizInfo
- Publication number
- BRPI0606363A2 BRPI0606363A2 BRPI0606363-2A BRPI0606363A BRPI0606363A2 BR PI0606363 A2 BRPI0606363 A2 BR PI0606363A2 BR PI0606363 A BRPI0606363 A BR PI0606363A BR PI0606363 A2 BRPI0606363 A2 BR PI0606363A2
- Authority
- BR
- Brazil
- Prior art keywords
- nucleic acids
- matrix
- isolating
- immobilizing
- nucleic acid
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 5
- 102000039446 nucleic acids Human genes 0.000 title abstract 5
- 108020004707 nucleic acids Proteins 0.000 title abstract 5
- 150000007523 nucleic acids Chemical class 0.000 title abstract 3
- 239000011159 matrix material Substances 0.000 title abstract 2
- 230000003100 immobilizing effect Effects 0.000 title 1
- 241000700605 Viruses Species 0.000 abstract 1
- 210000004102 animal cell Anatomy 0.000 abstract 1
- 230000001580 bacterial effect Effects 0.000 abstract 1
- 238000004113 cell culture Methods 0.000 abstract 1
- 210000005260 human cell Anatomy 0.000 abstract 1
- 238000009413 insulation Methods 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
MéTODO PARA O ISOLAMENTO E/OU A PURIFICAçãO DE áCIDOS NUCLEICOS áCIDO NUCLEICO, E, MéTODO PARA A IMOBILIZAçãO DE áCIDOS NUCLEICOS EM UMA MATRIZ. A presente invenção refere-se a um método aperfeiçoado para o isolamento de ácidos nucleicos como DNA e RNA de células bacterianas, de plantas, animais ou humanos, bem como de culturas de células e culturas de vírus.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP05002932A EP1690938A1 (de) | 2005-02-11 | 2005-02-11 | Verfahren zur Isolierung von Nukleinsäuren, wobei die Nukleinsäuren bei erhöhter Temperatur an einer Matrix immobilisiert werden |
| EP05002932.1 | 2005-02-11 | ||
| PCT/EP2006/001274 WO2006084753A1 (de) | 2005-02-11 | 2006-02-13 | Verfahren zur isolierung von nukleinsäuren, wobei die nukleinsäuren bei erhöhter temperatur an einer matrix immobilisiert werden |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| BRPI0606363A2 true BRPI0606363A2 (pt) | 2009-11-17 |
| BRPI0606363B1 BRPI0606363B1 (pt) | 2018-01-09 |
Family
ID=34933703
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0606363-2A BRPI0606363B1 (pt) | 2005-02-11 | 2006-02-13 | Método para o isolamento e/ou a purificação de ácidos nucleicos ácido nucleico, e, método para a imobilização de ácidos nucleicos em uma matriz |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US9410145B2 (pt) |
| EP (3) | EP1690938A1 (pt) |
| JP (1) | JP5588097B2 (pt) |
| CN (1) | CN101115833B (pt) |
| AU (1) | AU2006212392B2 (pt) |
| BR (1) | BRPI0606363B1 (pt) |
| CA (1) | CA2597573C (pt) |
| ES (1) | ES2616569T3 (pt) |
| WO (1) | WO2006084753A1 (pt) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1690938A1 (de) * | 2005-02-11 | 2006-08-16 | Qiagen GmbH | Verfahren zur Isolierung von Nukleinsäuren, wobei die Nukleinsäuren bei erhöhter Temperatur an einer Matrix immobilisiert werden |
| EP2128169A1 (de) * | 2008-05-30 | 2009-12-02 | Qiagen GmbH | Verfahren zur Isolierung von kurzkettigen Nukleinsäuren |
| CN101302508B (zh) * | 2008-06-12 | 2010-09-15 | 上海交通大学 | 假单胞菌m18的rna快速提取方法 |
| DE102008047790A1 (de) * | 2008-09-17 | 2010-04-15 | Qiagen Gmbh | Verfahren zur Normierung des Gehalts von Biomolekülen in einer Probe |
| EP2503316A1 (de) * | 2008-09-18 | 2012-09-26 | Qiagen GmbH | Verfahren und Vorrichtung für das zeitgleiche, automatisierte Aufschließen von mehreren biologischen Proben |
| DE102009022512A1 (de) * | 2009-05-25 | 2010-12-02 | Qiagen Gmbh | Verfahren zur Reaktivierung von Silikaoberflächen zur Isolierung von Nukleinsäuren |
| EP2345719A1 (en) * | 2010-01-18 | 2011-07-20 | Qiagen GmbH | Method for isolating small RNA |
| EP2407540A1 (en) * | 2010-07-15 | 2012-01-18 | Qiagen GmbH | Method for purifying a target nucleic acid |
| DE112011102524T5 (de) | 2010-07-29 | 2013-07-11 | Roche Diagnostics Gmbh | Präparation generischer Proben |
| US9725754B2 (en) | 2010-07-29 | 2017-08-08 | Sean F. Boyle | Generic sample preparation |
| US8722329B2 (en) | 2011-02-21 | 2014-05-13 | Rheonix, Inc. | Microfluidic device-based nucleic acid purification method |
| CN103764827B (zh) | 2011-07-04 | 2018-04-24 | 恰根有限公司 | 可用于分离和／或纯化核酸的试剂 |
| CN102864139A (zh) * | 2012-09-11 | 2013-01-09 | 西安交通大学口腔医院 | 一种用于组织细胞基因组dna的核酸吸附快速分离方法 |
| US10745686B2 (en) | 2013-02-08 | 2020-08-18 | Qiagen Gmbh | Method for separating DNA by size |
| AU2015336065A1 (en) | 2014-10-20 | 2017-05-04 | Pharmaceutical Manufacturing Research Services, Inc. | Extended release abuse deterrent liquid fill dosage form |
| PL3286325T3 (pl) * | 2015-04-23 | 2023-01-23 | Ist Innuscreen Gmbh | Sposób i zestaw testowy do izolowania kwasów nukleinowych za pomocą szorstkich powierzchni |
| JP7007197B2 (ja) | 2015-06-05 | 2022-01-24 | キアゲン ゲーエムベーハー | サイズによってｄｎａを分離する方法 |
| WO2016198519A1 (en) * | 2015-06-09 | 2016-12-15 | Biocartis N.V. | Automatable method for nucleic acid isolation |
| WO2017013102A1 (en) | 2015-07-23 | 2017-01-26 | Biocartis Nv | Optimized clinical sample sequencing |
| WO2018061877A1 (ja) * | 2016-09-30 | 2018-04-05 | 栄研化学株式会社 | 核酸を抽出する方法及びそれに用いるキット |
| CN109337309B (zh) * | 2018-08-30 | 2021-01-29 | 英芮诚生化科技（上海）有限公司 | 储水多孔二氧化硅磁性颗粒及其制备工艺与应用 |
| CN109215998B (zh) * | 2018-08-30 | 2020-11-10 | 英芮诚生化科技（上海）有限公司 | 改进磁性硅颗粒及其用于核酸纯化的方法 |
| CN120418429A (zh) * | 2022-12-15 | 2025-08-01 | 加拿大干细胞技术公司 | 用于核酸提取的方法 |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4321904B4 (de) * | 1993-07-01 | 2013-05-16 | Qiagen Gmbh | Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen |
| US6027945A (en) * | 1997-01-21 | 2000-02-22 | Promega Corporation | Methods of isolating biological target materials using silica magnetic particles |
| DE19743518A1 (de) * | 1997-10-01 | 1999-04-15 | Roche Diagnostics Gmbh | Automatisierbare universell anwendbare Probenvorbereitungsmethode |
| US5972613A (en) * | 1997-12-09 | 1999-10-26 | The Perkin-Elmer Corporation | Methods of nucleic acid isolation |
| DE19912799B4 (de) * | 1998-03-12 | 2004-12-30 | AGOWA Gesellschaft für molekularbiologische Technologie mbH | Superparamagnetisches Adsorptionsmaterial und seine Verwendung |
| DE19900638C2 (de) * | 1999-01-11 | 2002-12-19 | Max Planck Gesellschaft | Methode zur Isolierung von DNA aus biologischen Materialien |
| AU782305B2 (en) * | 1999-05-28 | 2005-07-21 | Enterprise Ireland Trading As Bioresearch Ireland | Nucleic acid probes and methods for detecting clinically important fungal pathogens |
| DE60015148T2 (de) * | 1999-08-20 | 2006-02-16 | Promega Corp., Madison | Simultane isolierung und quantifizierung von dna |
| US7005266B2 (en) * | 2000-02-04 | 2006-02-28 | Qiagen Gmbh | Nucleic acid isolation from stool samples and other inhibitor-rich biological materials |
| AU6380001A (en) * | 2000-03-24 | 2001-10-03 | Qiagen Gmbh | Porous ferro- or ferrimagnetic glass particles for isolating molecules |
| DE10033991A1 (de) * | 2000-07-12 | 2002-01-24 | Qiagen Gmbh | Verfahren zur Isolierung von Nukleinsäuren |
| JP3602071B2 (ja) * | 2001-06-05 | 2004-12-15 | 株式会社日立製作所 | 核酸の精製分離方法 |
| US7148343B2 (en) * | 2001-10-12 | 2006-12-12 | Gentra Systems, Inc. | Compositions and methods for using a solid support to purify RNA |
| JP4241183B2 (ja) * | 2003-05-19 | 2009-03-18 | 株式会社日立ハイテクノロジーズ | 核酸回収方法及び核酸回収キット |
| JP2004215676A (ja) * | 2004-04-26 | 2004-08-05 | Hitachi Ltd | 核酸の精製分離方法 |
| EP1690938A1 (de) * | 2005-02-11 | 2006-08-16 | Qiagen GmbH | Verfahren zur Isolierung von Nukleinsäuren, wobei die Nukleinsäuren bei erhöhter Temperatur an einer Matrix immobilisiert werden |
-
2005
- 2005-02-11 EP EP05002932A patent/EP1690938A1/de not_active Withdrawn
-
2006
- 2006-02-13 EP EP06706886.6A patent/EP1851313B1/de not_active Expired - Lifetime
- 2006-02-13 CN CN2006800040943A patent/CN101115833B/zh not_active Expired - Lifetime
- 2006-02-13 JP JP2007554513A patent/JP5588097B2/ja not_active Expired - Lifetime
- 2006-02-13 BR BRPI0606363-2A patent/BRPI0606363B1/pt active IP Right Grant
- 2006-02-13 ES ES06706886.6T patent/ES2616569T3/es not_active Expired - Lifetime
- 2006-02-13 US US11/815,296 patent/US9410145B2/en active Active
- 2006-02-13 AU AU2006212392A patent/AU2006212392B2/en not_active Expired
- 2006-02-13 EP EP16207254.0A patent/EP3168303A1/de not_active Withdrawn
- 2006-02-13 CA CA2597573A patent/CA2597573C/en not_active Expired - Lifetime
- 2006-02-13 WO PCT/EP2006/001274 patent/WO2006084753A1/de not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| EP3168303A1 (de) | 2017-05-17 |
| CA2597573A1 (en) | 2006-08-17 |
| BRPI0606363B1 (pt) | 2018-01-09 |
| JP5588097B2 (ja) | 2014-09-10 |
| CN101115833A (zh) | 2008-01-30 |
| CN101115833B (zh) | 2012-01-04 |
| US20080207889A1 (en) | 2008-08-28 |
| ES2616569T3 (es) | 2017-06-13 |
| EP1851313A1 (de) | 2007-11-07 |
| US9410145B2 (en) | 2016-08-09 |
| AU2006212392B2 (en) | 2012-01-12 |
| CA2597573C (en) | 2013-05-21 |
| WO2006084753A1 (de) | 2006-08-17 |
| EP1851313B1 (de) | 2017-01-04 |
| EP1690938A1 (de) | 2006-08-16 |
| AU2006212392A1 (en) | 2006-08-17 |
| JP2008529509A (ja) | 2008-08-07 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B06G | Technical and formal requirements: other requirements [chapter 6.7 patent gazette] |
Free format text: SOLICITA-SE A REGULARIZACAO DA PROCURACAO, UMA VEZ QUE BASEADO NO ARTIGO 216 1O DA LPI, O DOCUMENTO DE PROCURACAO DEVE SER APRESENTADO EM SUA FORMA AUTENTICADA; OU SEGUNDO O PARECER DA PROCURADORIA NO 074/93, DEVE CONSTAR UMA DECLARACAO DE VERACIDADE, A QUAL DEVE SER ASSINADA POR UMA PESSOA DEVIDAMENTE AUTORIZADA A REPRESENTAR O INTERESSADO, DEVENDO A MESMA CONSTAR NO INSTRUMENTO DE PROCURACAO, OU NO SEU SUBSTABELECIMENTO. |
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| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
| B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
| B09A | Decision: intention to grant [chapter 9.1 patent gazette] | ||
| B16A | Patent or certificate of addition of invention granted [chapter 16.1 patent gazette] |