ATE431408T1 - Methode zur trennung und aufreinigung von nukleinsäuren - Google Patents
Methode zur trennung und aufreinigung von nukleinsäurenInfo
- Publication number
- ATE431408T1 ATE431408T1 AT03015675T AT03015675T ATE431408T1 AT E431408 T1 ATE431408 T1 AT E431408T1 AT 03015675 T AT03015675 T AT 03015675T AT 03015675 T AT03015675 T AT 03015675T AT E431408 T1 ATE431408 T1 AT E431408T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acid
- nucleic acids
- purification
- separation
- desorbing
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title abstract 9
- 102000039446 nucleic acids Human genes 0.000 title abstract 9
- 108020004707 nucleic acids Proteins 0.000 title abstract 9
- 238000000034 method Methods 0.000 title abstract 3
- 238000000746 purification Methods 0.000 title 1
- 238000000926 separation method Methods 0.000 title 1
- 239000000203 mixture Substances 0.000 abstract 2
- 239000007790 solid phase Substances 0.000 abstract 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 abstract 1
- 229920002521 macromolecule Polymers 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2002210832A JP3890360B2 (ja) | 2002-07-19 | 2002-07-19 | 核酸の分離精製方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE431408T1 true ATE431408T1 (de) | 2009-05-15 |
Family
ID=29774668
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT03015675T ATE431408T1 (de) | 2002-07-19 | 2003-07-18 | Methode zur trennung und aufreinigung von nukleinsäuren |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US7432054B2 (de) |
| EP (1) | EP1382676B1 (de) |
| JP (1) | JP3890360B2 (de) |
| AT (1) | ATE431408T1 (de) |
| DE (1) | DE60327583D1 (de) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005095003A (ja) * | 2003-09-03 | 2005-04-14 | Fuji Photo Film Co Ltd | 核酸の分離精製方法 |
| JP2006238854A (ja) * | 2004-03-26 | 2006-09-14 | Fuji Photo Film Co Ltd | 核酸の分離精製方法 |
| WO2005093052A1 (en) | 2004-03-26 | 2005-10-06 | Fuji Photo Film Co., Ltd. | Method for selectively separating and purifying rna and method for separating and purifying nucleic acid |
| JP4810164B2 (ja) * | 2004-09-03 | 2011-11-09 | 富士フイルム株式会社 | 核酸分離精製方法 |
| JP2008527973A (ja) * | 2005-01-31 | 2008-07-31 | 富士フイルム株式会社 | 試料溶液調製方法および試料溶液調製装置 |
| US20070116613A1 (en) * | 2005-11-23 | 2007-05-24 | Donat Elsener | Sample tube and system for storing and providing nucleic acid samples |
| EP2128169A1 (de) * | 2008-05-30 | 2009-12-02 | Qiagen GmbH | Verfahren zur Isolierung von kurzkettigen Nukleinsäuren |
| US9464319B2 (en) | 2009-03-24 | 2016-10-11 | California Institute Of Technology | Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes |
| CA2756463C (en) | 2009-03-24 | 2019-01-22 | University Of Chicago | Slip chip device and methods |
| US10196700B2 (en) | 2009-03-24 | 2019-02-05 | University Of Chicago | Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes |
| US9447461B2 (en) | 2009-03-24 | 2016-09-20 | California Institute Of Technology | Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4118336A (en) * | 1974-03-25 | 1978-10-03 | Toyo Jozo Company, Ltd. | Novel cellulose microcapsules and preparation thereof |
| US4063017A (en) * | 1976-04-22 | 1977-12-13 | Purdue Research Foundation | Porous cellulose beads and the immobilization of enzymes therewith |
| US5187083A (en) * | 1990-11-13 | 1993-02-16 | Specialty Laboratories, Inc. | Rapid purification of DNA |
| US5491068A (en) * | 1991-02-14 | 1996-02-13 | Vicam, L.P. | Assay method for detecting the presence of bacteria |
| CA2067711C (en) * | 1991-05-03 | 2000-08-08 | Daniel Lee Woodard | Solid phase extraction purification of dna |
| DE69326507T2 (de) * | 1992-07-02 | 2000-04-27 | Edge Biosystems Inc., Gaithersburg | Verfahren und Material für Reinigung von Nukleinsäuren |
| US5804684A (en) | 1995-08-24 | 1998-09-08 | The Theobald Smith Research Institute, Inc. | Method for isolating nucleic acids |
| US6056877A (en) * | 1997-12-05 | 2000-05-02 | Transgenomic, Inc. | Non-polar media for polynucleotide separations |
| JP2001516731A (ja) * | 1997-09-17 | 2001-10-02 | ジエントラ・システムズ・インコーポレーテツド | 核酸を単離するための装置および方法 |
| JP3890556B2 (ja) | 2001-11-28 | 2007-03-07 | 富士フイルム株式会社 | 核酸の分離精製方法及び核酸分離精製ユニット |
-
2002
- 2002-07-19 JP JP2002210832A patent/JP3890360B2/ja not_active Expired - Fee Related
-
2003
- 2003-07-18 EP EP03015675A patent/EP1382676B1/de not_active Expired - Lifetime
- 2003-07-18 US US10/621,715 patent/US7432054B2/en not_active Expired - Fee Related
- 2003-07-18 DE DE60327583T patent/DE60327583D1/de not_active Expired - Fee Related
- 2003-07-18 AT AT03015675T patent/ATE431408T1/de not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| JP2004049106A (ja) | 2004-02-19 |
| US20040063122A1 (en) | 2004-04-01 |
| US7432054B2 (en) | 2008-10-07 |
| EP1382676B1 (de) | 2009-05-13 |
| EP1382676A1 (de) | 2004-01-21 |
| DE60327583D1 (de) | 2009-06-25 |
| JP3890360B2 (ja) | 2007-03-07 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |