DK1159453T3 - Fremgangsmåde til direkte nukleinsyresekventering - Google Patents

Fremgangsmåde til direkte nukleinsyresekventering

Info

Publication number
DK1159453T3
DK1159453T3 DK00909464T DK00909464T DK1159453T3 DK 1159453 T3 DK1159453 T3 DK 1159453T3 DK 00909464 T DK00909464 T DK 00909464T DK 00909464 T DK00909464 T DK 00909464T DK 1159453 T3 DK1159453 T3 DK 1159453T3
Authority
DK
Denmark
Prior art keywords
nucleic acid
acid sequencing
direct nucleic
acid molecules
present
Prior art date
Application number
DK00909464T
Other languages
English (en)
Inventor
Derek Lyle Stemple
Niall Antony Armes
Original Assignee
Asm Scient Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Asm Scient Inc filed Critical Asm Scient Inc
Application granted granted Critical
Publication of DK1159453T3 publication Critical patent/DK1159453T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y10/00—Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Nanotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Mathematical Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Medical Informatics (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK00909464T 1999-03-10 2000-03-10 Fremgangsmåde til direkte nukleinsyresekventering DK1159453T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US26618799A 1999-03-10 1999-03-10
PCT/GB2000/000873 WO2000053805A1 (en) 1999-03-10 2000-03-10 A method for direct nucleic acid sequencing

Publications (1)

Publication Number Publication Date
DK1159453T3 true DK1159453T3 (da) 2008-10-06

Family

ID=23013538

Family Applications (1)

Application Number Title Priority Date Filing Date
DK00909464T DK1159453T3 (da) 1999-03-10 2000-03-10 Fremgangsmåde til direkte nukleinsyresekventering

Country Status (11)

Country Link
US (1) US7270951B1 (da)
EP (2) EP1961826A3 (da)
JP (1) JP2002537858A (da)
AT (1) ATE397092T1 (da)
AU (1) AU3174600A (da)
CA (1) CA2366112A1 (da)
DE (1) DE60039037D1 (da)
DK (1) DK1159453T3 (da)
ES (1) ES2310514T3 (da)
PT (1) PT1159453E (da)
WO (1) WO2000053805A1 (da)

Families Citing this family (124)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL156002A0 (en) 1997-02-12 2003-12-23 Eugene Y Chan Methods and products for analyzing polymers
JP4294740B2 (ja) * 1997-05-23 2009-07-15 ソレクサ・インコーポレイテッド 分析物の系列的プロセシングのためのシステムおよび装置
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
WO2001016375A2 (en) * 1999-08-30 2001-03-08 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
US6982146B1 (en) 1999-08-30 2006-01-03 The United States Of America As Represented By The Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
US6908736B1 (en) 1999-10-06 2005-06-21 Medical Biosystems, Ltd. DNA sequencing method
GB9923644D0 (en) * 1999-10-06 1999-12-08 Medical Biosystems Ltd DNA sequencing
EP2100971A3 (en) * 2000-07-07 2009-11-25 Visigen Biotechnologies, Inc. Real-time sequence determination
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
EP3034627B1 (en) 2000-10-06 2019-01-30 The Trustees of Columbia University in the City of New York Massive parallel method for decoding dna and rna
US7211414B2 (en) 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
JP2004523243A (ja) 2001-03-12 2004-08-05 カリフォルニア インスティチュート オブ テクノロジー 非同期性塩基伸長によってポリヌクレオチド配列を分析するための方法および装置
DE10120797B4 (de) * 2001-04-27 2005-12-22 Genovoxx Gmbh Verfahren zur Analyse von Nukleinsäureketten
GB0112238D0 (en) 2001-05-18 2001-07-11 Medical Biosystems Ltd Sequencing method
US7668697B2 (en) 2006-02-06 2010-02-23 Andrei Volkov Method for analyzing dynamic detectable events at the single molecule level
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
GB0129012D0 (en) 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
US7074597B2 (en) 2002-07-12 2006-07-11 The Trustees Of Columbia University In The City Of New York Multiplex genotyping using solid phase capturable dideoxynucleotides and mass spectrometry
US11008359B2 (en) 2002-08-23 2021-05-18 Illumina Cambridge Limited Labelled nucleotides
EP3147292B1 (en) 2002-08-23 2018-09-26 Illumina Cambridge Limited Labelled nucleotides
AU2003259350A1 (en) 2002-08-23 2004-03-11 Solexa Limited Modified nucleotides for polynucleotide sequencing
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
DE602004026033D1 (de) * 2003-01-29 2010-04-29 454 Corp Zweiendige sequenzierung
US7745116B2 (en) * 2003-04-08 2010-06-29 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
GB0317343D0 (en) * 2003-07-24 2003-08-27 Medical Biosystems Ltd Polynucleotide sequencing
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
ATE463584T1 (de) 2004-02-19 2010-04-15 Helicos Biosciences Corp Verfahren zur analyse von polynukleotidsequenzen
US7622279B2 (en) 2004-03-03 2009-11-24 The Trustees Of Columbia University In The City Of New York Photocleavable fluorescent nucleotides for DNA sequencing on chip constructed by site-specific coupling chemistry
US20070077575A1 (en) * 2004-03-05 2007-04-05 Mcallister William T Use of rna polymerase as an information-dependent molecular motor
EP1766090B1 (en) * 2004-05-25 2011-04-27 Helicos Biosciences Corporation Methods for nucleic acid immobilisation
US7476734B2 (en) 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
WO2005118608A2 (en) * 2004-06-02 2005-12-15 Asm Scientific, Inc. 2’-nitrobenzyl-modified ribonucleotides
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
US7315019B2 (en) * 2004-09-17 2008-01-01 Pacific Biosciences Of California, Inc. Arrays of optical confinements and uses thereof
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US7482120B2 (en) 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
AU2006211150A1 (en) * 2005-01-31 2006-08-10 Pacific Biosciences Of California, Inc. Use of reversible extension terminator in nucleic acid sequencing
US9777314B2 (en) * 2005-04-21 2017-10-03 Esoterix Genetic Laboratories, Llc Analysis of heterogeneous nucleic acid samples
EP2278303A3 (en) * 2005-06-10 2012-02-22 Life Technologies Corporation Method and system for multiplex genetic analysis
WO2007002204A2 (en) 2005-06-21 2007-01-04 The Trustees Of Columbia University In The City Of New York Pyrosequencing methods and related compostions
GB0517097D0 (en) 2005-08-19 2005-09-28 Solexa Ltd Modified nucleosides and nucleotides and uses thereof
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
US7763423B2 (en) 2005-09-30 2010-07-27 Pacific Biosciences Of California, Inc. Substrates having low density reactive groups for monitoring enzyme activity
WO2007053702A2 (en) 2005-10-31 2007-05-10 The Trustees Of Columbia University In The City Of New York Synthesis of four color 3'-o-allyl modified photocleavable fluorescent nucleotides and related methods
US8796432B2 (en) 2005-10-31 2014-08-05 The Trustees Of Columbia University In The City Of New York Chemically cleavable 3'-o-allyl-DNTP-allyl-fluorophore fluorescent nucleotide analogues and related methods
US8703734B2 (en) 2005-12-12 2014-04-22 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Nanoprobes for detection or modification of molecules
JP2009519041A (ja) 2005-12-12 2009-05-14 アメリカ合衆国 核酸配列決定のためのプローブおよび使用方法
CN101346472B (zh) * 2005-12-21 2013-04-24 霍夫曼—拉罗奇有限公司 用可逆2’-修饰的核苷酸进行的序列测定和基因分型
JP2009521227A (ja) 2005-12-22 2009-06-04 パシフィック バイオサイエンシーズ オブ カリフォルニア, インコーポレイテッド ヌクレオチドアナログ組込み用ポリメラーゼ
CA2633520A1 (en) * 2005-12-22 2007-07-05 Pacific Biosciences Of California, Inc. Protein engineering strategies to optimize activity of surface attached proteins
AU2006331512B2 (en) * 2005-12-22 2012-02-23 Pacific Biosciences Of California, Inc. Active surface coupled polymerases
US7397546B2 (en) 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
US20080076189A1 (en) * 2006-03-30 2008-03-27 Visigen Biotechnologies, Inc. Modified surfaces for the detection of biomolecules at the single molecule level
US8975216B2 (en) 2006-03-30 2015-03-10 Pacific Biosciences Of California Articles having localized molecules disposed thereon and methods of producing same
SG170802A1 (en) 2006-03-31 2011-05-30 Solexa Inc Systems and devices for sequence by synthesis analysis
AU2007260707A1 (en) * 2006-06-16 2007-12-21 Pacific Biosciences Of California, Inc. Controlled initiation of primer extension
US20080241938A1 (en) * 2006-07-20 2008-10-02 Visigen Biotechnologies, Inc. Automated synthesis or sequencing apparatus and method for making and using same
EP2071927A2 (en) 2006-09-28 2009-06-24 Illumina, Inc. Compositions and methods for nucleotide sequencing
EP2089517A4 (en) 2006-10-23 2010-10-20 Pacific Biosciences California POLYMERASEENZYME AND REAGENTS FOR ADVANCED NUCKIC ACID SEQUENCING
GB2457402B (en) 2006-12-01 2011-10-19 Univ Columbia Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
US11940413B2 (en) 2007-02-05 2024-03-26 IsoPlexis Corporation Methods and devices for sequencing nucleic acids in smaller batches
US20080241866A1 (en) * 2007-03-30 2008-10-02 Pacific Biosciences Of California, Inc. Systems and methods for enhancing fluorescent signals
EP2209911B1 (en) 2007-10-19 2013-10-16 The Trustees of Columbia University in the City of New York Dna sequencing with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators and a deoxyinosine analogue with a reversible terminator group
EP2207900B1 (en) 2007-10-19 2015-04-29 The Trustees of Columbia University in the City of New York Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequencing by synthesis
US8017338B2 (en) 2007-11-20 2011-09-13 Life Technologies Corporation Reversible di-nucleotide terminator sequencing
JP2011515102A (ja) 2008-03-28 2011-05-19 パシフィック バイオサイエンシーズ オブ カリフォルニア, インコーポレイテッド 核酸シーケンシング用組成物及び方法
US20090247426A1 (en) * 2008-03-31 2009-10-01 Pacific Biosciences Of California, Inc. Focused library generation
US8911948B2 (en) 2008-04-30 2014-12-16 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
EP3150727B1 (en) * 2008-04-30 2019-07-10 Integrated DNA Technologies Inc. Rnase-h-based assays utilizing modified rna monomers
US9434988B2 (en) 2008-04-30 2016-09-06 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
WO2010005550A1 (en) * 2008-07-07 2010-01-14 The Regents Of The University Of California Caged quantum dots
US8795961B2 (en) * 2008-09-05 2014-08-05 Pacific Biosciences Of California, Inc. Preparations, compositions, and methods for nucleic acid sequencing
SG172965A1 (en) 2009-01-13 2011-08-29 Fluidigm Corp Single-cell nucleic acid analysis
WO2010111674A2 (en) * 2009-03-27 2010-09-30 Life Technologies Corporation Methods and apparatus for single molecule sequencing using energy transfer detection
US8501405B2 (en) 2009-04-27 2013-08-06 Pacific Biosciences Of California, Inc. Real-time sequencing methods and systems
WO2010141391A2 (en) 2009-06-05 2010-12-09 Life Technologies Corporation Mutant dna polymerases
US8501406B1 (en) 2009-07-14 2013-08-06 Pacific Biosciences Of California, Inc. Selectively functionalized arrays
US8603741B2 (en) 2010-02-18 2013-12-10 Pacific Biosciences Of California, Inc. Single molecule sequencing with two distinct chemistry steps
US8735175B2 (en) * 2011-03-18 2014-05-27 Chris D. Geddes Multicolor microwave-accelerated metal-enhanced fluorescence (M-MAMEF)
WO2012162429A2 (en) 2011-05-23 2012-11-29 The Trustees Of Columbia University In The City Of New York Dna sequencing by synthesis using raman and infrared spectroscopy detection
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
WO2014144883A1 (en) 2013-03-15 2014-09-18 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
US9591268B2 (en) 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems
ES2685549T3 (es) 2013-03-15 2018-10-09 Illumina Cambridge Limited Nucleósidos o nucleótidos modificados
US20150132743A1 (en) 2013-09-04 2015-05-14 Fluidigm Corporation Proximity assays for detecting nucleic acids and proteins in a single cell
EP3709003A1 (en) 2013-11-17 2020-09-16 Quantum-Si Incorporated Optical system and assay chip for probing, detecting and analyzing molecules
US10619202B2 (en) * 2013-11-29 2020-04-14 Koninklijke Philips N.V. Optical controlling of a chemical reaction
FR3020071B1 (fr) 2014-04-17 2017-12-22 Dna Script Procede de synthese d'acides nucleiques, notamment d'acides nucleiques de grande longueur, utilisation du procede et kit pour la mise en œuvre du procede
US20170101675A1 (en) * 2014-05-19 2017-04-13 The Trustees Of Columbia University In The City Of New York Ion sensor dna and rna sequencing by synthesis using nucleotide reversible terminators
CN111710687B (zh) 2014-08-08 2023-11-14 宽腾矽公司 基于入射光子到达时间的识别、成像、测序法及存储介质
US10174363B2 (en) 2015-05-20 2019-01-08 Quantum-Si Incorporated Methods for nucleic acid sequencing
KR102738114B1 (ko) 2014-08-08 2024-12-06 퀀텀-에스아이 인코포레이티드 분자들을 프로빙, 검출 및 분석하기 위한 광학계 및 검정 칩
US12655467B2 (en) 2014-08-08 2026-06-16 Quantum-Si Incorporated Methods for nucleic acid sequencing
US9885657B2 (en) 2014-08-08 2018-02-06 Quantum-Si Incorporated Integrated device with external light source for probing detecting and analyzing molecules
FR3025201B1 (fr) 2014-09-02 2018-10-12 Dna Script Nucleotides modifies pour la synthese d'acides nucleiques, un kit renfermant de tels nucleotides et leur utilisation pour la production de genes ou sequences d'acides nucleiques synthetiques
CN114350752B (zh) 2015-07-17 2025-02-25 布鲁克空间生物学公司 在横切面组织的用户定义区域中的基因表达的同时定量
TW202139919A (zh) 2016-02-17 2021-11-01 美商太斯萊特健康股份有限公司 用於生命期成像及偵測應用之感測器及裝置
CN116200462B (zh) * 2016-05-16 2025-10-03 布鲁克空间生物学公司 用于检测样品中目标核酸的方法
JP2019522976A (ja) 2016-06-22 2019-08-22 ザ チャールズ スターク ドレイパー ラボラトリー, インク.The Charles Stark Draper Laboratory, Inc. 分子鎖合成に使用するための組成物
WO2018064297A1 (en) * 2016-09-29 2018-04-05 Ultima Genomics, Inc. Systems and methods for nucleic acid sequencing
AU2017361521B2 (en) 2016-11-21 2020-08-27 Bruker Spatial Biology, Inc. Chemical compositions and methods of using same
CN110168732B (zh) 2016-12-22 2024-03-08 宽腾矽公司 具有直接合并像素的整合式光电侦测器
WO2018129328A1 (en) * 2017-01-06 2018-07-12 The Charles Stark Draper Laboratory, Inc. Optically verified polymer synthesis
CA3069983A1 (en) 2017-07-24 2019-01-31 Quantum-Si Incorporated Hand-held, massively-parallel, bio-optoelectronic instrument
WO2019030149A1 (en) 2017-08-07 2019-02-14 Dna Script VARIANTS OF FAMILY A DNA POLYMERASE AND USES THEREOF
US10752887B2 (en) 2018-01-08 2020-08-25 Dna Script Variants of terminal deoxynucleotidyl transferase and uses thereof
WO2019135007A1 (en) 2018-01-08 2019-07-11 Dna Script Variants of terminal deoxynucleotidyl transferase and uses thereof
KR102893736B1 (ko) 2018-05-14 2025-11-28 브루커 스페이셜 바이올로지, 인크. 화학 조성물 및 이의 사용 방법
MX2020013788A (es) 2018-06-22 2021-03-02 Quantum Si Inc Fotodetector integrado con deposito de almacenamiento de carga con tiempo de deteccion variado.
JP7430701B2 (ja) 2018-07-23 2024-02-13 ディーエヌエー スクリプト 核酸鎖の大規模並列酵素合成
MY204234A (en) 2018-08-27 2024-08-16 Regeneron Pharma Use of raman spectroscopy in downstream purification
SG11202105441WA (en) 2018-12-13 2021-06-29 Dna Script Direct oligonucleotide synthesis on cells and biomolecules
US11293061B2 (en) 2018-12-26 2022-04-05 Illumina Cambridge Limited Sequencing methods using nucleotides with 3′ AOM blocking group
WO2020165137A1 (en) 2019-02-12 2020-08-20 Dna Script Efficient product cleavage in template-free enzymatic synthesis of polynucleotides.
CN119662792A (zh) 2019-02-19 2025-03-21 阿尔缇玛基因组学公司 用于光学检测和测序的接头和方法
US11807851B1 (en) 2020-02-18 2023-11-07 Ultima Genomics, Inc. Modified polynucleotides and uses thereof
DE102020110587A1 (de) 2020-04-17 2021-10-21 Druckhaus Waiblingen Remstal-Bote Gesellschaft mit beschränkter Haftung Maske für das Abdecken eines Teilbereichs eines Gesichts
IL297992B2 (en) 2020-06-22 2025-03-01 Illumina Cambridge Ltd Nucleosides and nucleotides with 3' acetal blocking group
US20220154273A1 (en) * 2020-11-16 2022-05-19 Illumina, Inc. Incorporation and imaging mixes
WO2023212737A2 (en) * 2022-04-29 2023-11-02 454 Corporation Methods and devices for nucleic acid sequencing

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2102005B (en) * 1981-07-23 1984-11-21 Mta Koezponti Kemiai Kutato In Process for synthesis of polydeoxynucleotides
US5171534A (en) 1984-01-16 1992-12-15 California Institute Of Technology Automated DNA sequencing technique
US5821058A (en) 1984-01-16 1998-10-13 California Institute Of Technology Automated DNA sequencing technique
US4811218A (en) 1986-06-02 1989-03-07 Applied Biosystems, Inc. Real time scanning electrophoresis apparatus for DNA sequencing
SE462454B (sv) 1988-11-10 1990-06-25 Pharmacia Ab Maetyta foer anvaendning i biosensorer
GB8910880D0 (en) * 1989-05-11 1989-06-28 Amersham Int Plc Sequencing method
US5547839A (en) 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
US5302509A (en) 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
WO1991006678A1 (en) 1989-10-26 1991-05-16 Sri International Dna sequencing
CA2079005A1 (en) 1990-04-11 1991-10-12 Geoffrey Stephen Begg Methods and apparatus allowing sequential chemical reactions
US5405747A (en) 1991-09-25 1995-04-11 The Regents Of The University Of California Office Of Technology Transfer Method for rapid base sequencing in DNA and RNA with two base labeling
GB9208733D0 (en) * 1992-04-22 1992-06-10 Medical Res Council Dna sequencing method
JPH08507926A (ja) * 1993-03-19 1996-08-27 シーケノム・インコーポレーテツド エキソヌクレアーゼ分解を介した質量分析法によるdna配列決定
FR2703052B1 (fr) 1993-03-26 1995-06-02 Pasteur Institut Nouvelle méthode de séquençage d'acides nucléiques.
US6007987A (en) 1993-08-23 1999-12-28 The Trustees Of Boston University Positional sequencing by hybridization
GB9507238D0 (en) 1995-04-07 1995-05-31 Isis Innovation Detecting dna sequence variations
US5728529A (en) 1995-06-23 1998-03-17 Baylor College Of Medicine Alternative dye-labeled ribonucleotides, deoxyribonucleotides, and dideoxyribonucleotides for automated DNA analysis
US5856174A (en) 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
US5814506A (en) 1995-08-02 1998-09-29 New England Biolabs, Inc. Over-expression and purification of a truncated thermostable DNA polymerase by protein fusion
US5716825A (en) 1995-11-01 1998-02-10 Hewlett Packard Company Integrated nucleic acid analysis system for MALDI-TOF MS
US5830714A (en) 1996-04-17 1998-11-03 Molecular Biology Resources, Inc. Biologically active fragment of bacillus stearothermophilus DNA polymerase
US5846727A (en) 1996-06-06 1998-12-08 Board Of Supervisors Of Louisiana State University And Agricultural & Mechanical College Microsystem for rapid DNA sequencing
US5858671A (en) 1996-11-01 1999-01-12 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
WO1998033939A1 (en) * 1997-01-31 1998-08-06 Hitachi, Ltd. Method for determining nucleic acid base sequence and apparatus therefor
US5919626A (en) 1997-06-06 1999-07-06 Orchid Bio Computer, Inc. Attachment of unmodified nucleic acids to silanized solid phase surfaces
WO1999005315A2 (en) 1997-07-28 1999-02-04 Medical Biosystems Ltd. Nucleic acid sequence analysis
US6210896B1 (en) 1998-08-13 2001-04-03 Us Genomics Molecular motors
WO2000036152A1 (en) * 1998-12-14 2000-06-22 Li-Cor, Inc. A system and methods for nucleic acid sequencing of single molecules by polymerase synthesis

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EP1159453A1 (en) 2001-12-05
PT1159453E (pt) 2008-08-29
ATE397092T1 (de) 2008-06-15
EP1961826A3 (en) 2008-09-17
DE60039037D1 (de) 2008-07-10
CA2366112A1 (en) 2000-09-14
AU3174600A (en) 2000-09-28
JP2002537858A (ja) 2002-11-12
EP1961826A2 (en) 2008-08-27
EP1159453B1 (en) 2008-05-28
ES2310514T3 (es) 2009-01-16
WO2000053805A1 (en) 2000-09-14
US7270951B1 (en) 2007-09-18

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