ATE438724T1 - Chemisch modifizierte enzymen mit mehrfachgeladenen varianten - Google Patents

Chemisch modifizierte enzymen mit mehrfachgeladenen varianten

Info

Publication number
ATE438724T1
ATE438724T1 AT99966472T AT99966472T ATE438724T1 AT E438724 T1 ATE438724 T1 AT E438724T1 AT 99966472 T AT99966472 T AT 99966472T AT 99966472 T AT99966472 T AT 99966472T AT E438724 T1 ATE438724 T1 AT E438724T1
Authority
AT
Austria
Prior art keywords
chemically modified
modified enzymes
multiple charged
cysteine residues
enzymes
Prior art date
Application number
AT99966472T
Other languages
English (en)
Inventor
Benjamin Davis
John Jones
Richard Bott
Original Assignee
Genencor Int
Univ Toronto
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Int, Univ Toronto filed Critical Genencor Int
Application granted granted Critical
Publication of ATE438724T1 publication Critical patent/ATE438724T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6402—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals
    • C12N9/6405—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals not being snakes
    • C12N9/6408—Serine endopeptidases (3.4.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/33—Chemical structure of the base
    • C12N2310/334—Modified C

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Peptides Or Proteins (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT99966472T 1998-12-21 1999-12-20 Chemisch modifizierte enzymen mit mehrfachgeladenen varianten ATE438724T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US11313098P 1998-12-21 1998-12-21
PCT/US1999/030362 WO2000037658A2 (en) 1998-12-21 1999-12-20 Chemically modified enzymes with multiple charged variants
US09/467,536 US6379942B1 (en) 1998-12-21 1999-12-20 Chemically modified enzymes with multiple charged variants

Publications (1)

Publication Number Publication Date
ATE438724T1 true ATE438724T1 (de) 2009-08-15

Family

ID=26810726

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99966472T ATE438724T1 (de) 1998-12-21 1999-12-20 Chemisch modifizierte enzymen mit mehrfachgeladenen varianten

Country Status (9)

Country Link
US (3) US6379942B1 (de)
EP (1) EP1141335B1 (de)
JP (1) JP2002533087A (de)
AT (1) ATE438724T1 (de)
AU (1) AU2200100A (de)
CA (1) CA2353789C (de)
DE (1) DE69941223D1 (de)
DK (1) DK1141335T3 (de)
WO (1) WO2000037658A2 (de)

Families Citing this family (38)

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MA23346A1 (fr) * 1993-10-14 1995-04-01 Genencor Int Variantes de la subtilisine
ES2368718T3 (es) * 1997-10-23 2011-11-21 Danisco Us Inc. Variantes de subtilisina con múltiples sustituciones.
DE69837559T3 (de) * 1997-12-24 2011-07-21 Genencor International, Inc., Calif. Verfahren zur Bestimmung der Waschleistung eines Enzyms.
US6569663B1 (en) 1998-03-26 2003-05-27 The Procter & Gamble Company Serine protease variants having amino acid substitutions
US6908757B1 (en) 1998-03-26 2005-06-21 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
US6495136B1 (en) 1998-03-26 2002-12-17 The Procter & Gamble Company Proteases having modified amino acid sequences conjugated to addition moieties
WO2000028007A2 (en) * 1998-11-10 2000-05-18 Genencor International, Inc. Chemically modified mutant serine hydrolases
EP1185302B1 (de) * 1999-04-28 2013-07-24 Genencor International, Inc. Spezifischer zielrichtung von katalytische antagoniste und deren verwendungen
BR0012660A (pt) 1999-07-22 2002-04-09 Procter & Gamble Variante de protease tipo subtilisina; composição de limpeza; e composição de cuidado pessoal
CN1373802A (zh) 1999-07-22 2002-10-09 宝洁公司 在特定表位区域具有氨基酸缺失和取代的枯草杆菌蛋白酶变体
MXPA02000842A (es) 1999-07-22 2002-07-30 Procter & Gamble Conjugados de proteasa que tienen sitios de corte protegidos estericamente.
EP1196547A2 (de) * 1999-07-22 2002-04-17 The Procter & Gamble Company Proteasekonjugate mit sterisch geschützten epitopgebieten
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
US6727085B2 (en) 1999-12-15 2004-04-27 Fanoe Tina Sejersgaard Subtilase variants having an improved wash performance on egg stains
EP1244779B1 (de) * 1999-12-15 2014-05-07 Novozymes A/S Subtilasevarianten mit verbesserter waschleistung bei eierflecken
AU758744B2 (en) * 2000-10-31 2003-03-27 F. Hoffmann-La Roche Ag Methods for the analysis of non-proteinaceous components using a protease from a bacillus strain
US20040097720A1 (en) * 2001-04-02 2004-05-20 Bott Richard R. Use of glycodendrimer reagents for inhibiting adhesion by microorganisms
DE10206620B4 (de) * 2002-02-15 2005-08-18 Henkel Kgaa Vorrichtung und Verfahren zum gleichzeitigen Testen der Einwirkung von Flüssigkeiten auf Flächengebilde sowie Verwendung der Vorrichtung
KR100475133B1 (ko) * 2002-09-13 2005-03-10 한국생명공학연구원 효모 표면 발현 벡터를 이용하여 변이 리파제를스크리닝하는 방법 및 신규 변이 리파제
TWI319007B (en) * 2002-11-06 2010-01-01 Novozymes As Subtilase variants
US7888093B2 (en) 2002-11-06 2011-02-15 Novozymes A/S Subtilase variants
US9592277B2 (en) * 2004-04-14 2017-03-14 Avirid, Inc. Compositions with modified nucleases targeted to viral nucleic acids and methods of use for prevention and treatment of viral diseases
WO2006063587A1 (en) * 2004-12-14 2006-06-22 Novozymes A/S Testing the activity of an enzyme using absorbance
US8232267B2 (en) * 2006-10-06 2012-07-31 The Trustees Of Princeton University Porphyrin catalysts and methods of use thereof
CN102282155B (zh) 2008-12-02 2017-06-09 日本波涛生命科学公司 磷原子修饰的核酸的合成方法
RU2612521C2 (ru) 2009-07-06 2017-03-09 Онтории, Инк. Новые пролекарства нуклеиновых кислот и способы их применения
WO2011075185A1 (en) 2009-12-18 2011-06-23 Oligasis Targeted drug phosphorylcholine polymer conjugates
BR112012026118B1 (pt) 2010-04-15 2021-01-05 Oligasis polímeros de alto peso molecular contendo zwitterion
JP5868324B2 (ja) 2010-09-24 2016-02-24 株式会社Wave Life Sciences Japan 不斉補助基
CN103796657B (zh) * 2011-07-19 2017-07-11 波涛生命科学有限公司 合成官能化核酸的方法
CA2862450A1 (en) 2011-12-30 2013-07-04 Butamax Advanced Biofuels Llc Genetic switches for butanol production
CA2879066C (en) 2012-07-13 2019-08-13 Shin Nippon Biomedical Laboratories, Ltd. Chiral nucleic acid adjuvant
PL2872485T3 (pl) 2012-07-13 2021-05-31 Wave Life Sciences Ltd. Asymetryczna grupa pomocnicza
KR102213609B1 (ko) 2012-07-13 2021-02-08 웨이브 라이프 사이언시스 리미티드 키랄 제어
US10322173B2 (en) 2014-01-15 2019-06-18 Shin Nippon Biomedical Laboratories, Ltd. Chiral nucleic acid adjuvant having anti-allergic activity, and anti-allergic agent
JPWO2015108048A1 (ja) 2014-01-15 2017-03-23 株式会社新日本科学 抗腫瘍作用を有するキラル核酸アジュバンド及び抗腫瘍剤
JPWO2015108047A1 (ja) 2014-01-15 2017-03-23 株式会社新日本科学 免疫誘導活性を有するキラル核酸アジュバンド及び免疫誘導活性剤
CA3157509A1 (en) 2019-10-10 2021-04-15 Kodiak Sciences Inc. Methods of treating an eye disorder

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5155033A (en) 1989-01-06 1992-10-13 Genencor, Inc. Subtilisins modified at position 225 resulting in a shift in catalytic activity
US5244791A (en) 1984-05-29 1993-09-14 Genecor International, Inc. Methods of ester hydrolysis
US5260207A (en) * 1987-04-06 1993-11-09 Enzon Labs Inc. Engineering of electrostatic interactions at metal ion binding sites for the stabilization of proteins
CN1056187C (zh) 1988-02-11 2000-09-06 金克克国际有限公司 新的蛋白水解酶及其在洗涤剂中的应用
DK97190D0 (da) 1990-04-19 1990-04-19 Novo Nordisk As Oxidationsstabile detergentenzymer
US5629173A (en) 1990-08-09 1997-05-13 Genentech, Inc. Methods of use of serine protease variants having peptide ligase activity
EP0542931B1 (de) 1990-08-09 1999-12-08 Genentech, Inc. Serinproteasevarianten mit peptidligase aktivität
US5316935A (en) 1992-04-06 1994-05-31 California Institute Of Technology Subtilisin variants suitable for hydrolysis and synthesis in organic media
MA23346A1 (fr) 1993-10-14 1995-04-01 Genencor Int Variantes de la subtilisine
US5837516A (en) 1995-03-03 1998-11-17 Genentech, Inc. Subtilisin variants capable of cleaving substrates containing basic residues
US5837517A (en) 1995-05-05 1998-11-17 Novo Nordisk A/S Protease variants and compositions
US6946280B1 (en) 1996-03-29 2005-09-20 Genencor International, Inc. Enzyme multimer and process of producing same
CN1246146A (zh) * 1996-11-26 2000-03-01 金克克国际有限公司 化学修饰的酶

Also Published As

Publication number Publication date
JP2002533087A (ja) 2002-10-08
US6379942B1 (en) 2002-04-30
WO2000037658A2 (en) 2000-06-29
EP1141335A2 (de) 2001-10-10
CA2353789C (en) 2012-02-14
DE69941223D1 (de) 2009-09-17
EP1141335B1 (de) 2009-08-05
US20050089966A1 (en) 2005-04-28
AU2200100A (en) 2000-07-12
WO2000037658A3 (en) 2000-11-09
DK1141335T3 (da) 2009-11-09
US20020127695A1 (en) 2002-09-12
US7371553B2 (en) 2008-05-13
CA2353789A1 (en) 2000-06-29

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