ATE439432T1 - Verfahren zur herstellung von säugetier-trypsinen - Google Patents

Verfahren zur herstellung von säugetier-trypsinen

Info

Publication number
ATE439432T1
ATE439432T1 AT03755759T AT03755759T ATE439432T1 AT E439432 T1 ATE439432 T1 AT E439432T1 AT 03755759 T AT03755759 T AT 03755759T AT 03755759 T AT03755759 T AT 03755759T AT E439432 T1 ATE439432 T1 AT E439432T1
Authority
AT
Austria
Prior art keywords
mammalian trypsin
nucleic acid
encoding
fusarium venenatum
fusarium
Prior art date
Application number
AT03755759T
Other languages
English (en)
Inventor
Randy Berka
Kimberly Brown
Original Assignee
Novozymes Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes Inc filed Critical Novozymes Inc
Application granted granted Critical
Publication of ATE439432T1 publication Critical patent/ATE439432T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21004—Trypsin (3.4.21.4)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
AT03755759T 2002-08-30 2003-08-29 Verfahren zur herstellung von säugetier-trypsinen ATE439432T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US40717002P 2002-08-30 2002-08-30
PCT/US2003/027074 WO2004020612A1 (en) 2002-08-30 2003-08-29 Methods for producing mammalian trypsins

Publications (1)

Publication Number Publication Date
ATE439432T1 true ATE439432T1 (de) 2009-08-15

Family

ID=31978430

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03755759T ATE439432T1 (de) 2002-08-30 2003-08-29 Verfahren zur herstellung von säugetier-trypsinen

Country Status (8)

Country Link
US (1) US20040043455A1 (de)
EP (1) EP1546302B1 (de)
CN (1) CN1694953B (de)
AT (1) ATE439432T1 (de)
AU (1) AU2003273256A1 (de)
DE (1) DE60328802D1 (de)
DK (1) DK1546302T3 (de)
WO (1) WO2004020612A1 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011047900A2 (en) 2009-10-22 2011-04-28 Glaxosmithkline Biologicals S.A. Novel process
CA2797059A1 (en) 2010-05-03 2011-11-10 Glaxosmithkline Biologicals S.A. Novel method
GB201011502D0 (en) 2010-07-08 2010-08-25 Glaxosmithkline Biolog Sa Novel process
JP6851827B2 (ja) 2013-08-30 2021-03-31 グラクソスミスクライン バイオロジカルズ ソシエテ アノニム 細胞培養中でのウイルスの大規模製造
WO2015181118A1 (en) 2014-05-27 2015-12-03 Novozymes A/S Methods for producing lipases
CN107090427A (zh) * 2017-06-23 2017-08-25 友康恒业生物科技（北京）有限公司 一种基因重组胰蛋白酶细胞消化液
CN113430187A (zh) * 2021-06-23 2021-09-24 山东黎宁科技新材料有限公司 一种皮革软化用复合酶及其制备方法与应用
CN115975996A (zh) * 2022-11-24 2023-04-18 中国科学院华南植物园 FpOPSB基因和蛋白在预防香蕉冠腐病中的应用

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
US5288627A (en) 1988-01-07 1994-02-22 Novo Nordisk A/S Endoprotease from Fusarium oxysporumDSM 2672 for use in detergents
DK52393D0 (de) 1993-05-05 1993-05-05 Novo Nordisk As
CN101659926A (zh) 1994-06-30 2010-03-03 诺沃奇梅兹有限公司 非毒性、非产毒性、非致病性镰孢属表达系统及所用启动子和终止子
WO1997000316A1 (en) 1995-06-16 1997-01-03 Novo Nordisk A/S A process for producing trypsin (trypsinogen)
US5945328A (en) * 1995-06-16 1999-08-31 Novo Nordisk A/S Process for producing trypsin (trypsinogen)
US5948746A (en) 1995-07-19 1999-09-07 Novo Nordisk A/S Proteolytic enzymes
CN1231588C (zh) 1996-01-19 2005-12-14 诺沃奇梅兹生物技术有限公司 丝状真菌的形态突变体
AU719536B2 (en) 1996-06-28 2000-05-11 Novozymes A/S A recombinant enzyme with dextranase activitiy
DE59812768D1 (de) 1997-08-22 2005-06-09 Roche Diagnostics Gmbh Autokatalytisch aktivierbare zymogene vorstufen von proteasen und deren verwendung
JP2002515253A (ja) * 1998-05-20 2002-05-28 ノボザイムス バイオテック，インコーポレイティド トリコテセン−欠失糸状菌変異体細胞において異種ポリベプチドを生成するための方法
WO2000017332A1 (en) 1998-09-21 2000-03-30 Eli Lilly And Company Production of soluble recombinant trypsinogen analogs
CN1197965C (zh) 1998-10-26 2005-04-20 诺维信公司 在丝状真菌细胞内构建和筛选目的dna文库
EP1144658B1 (de) 1999-01-13 2007-11-21 Novozymes, Inc. Verfahren zur herstellung von polypeptiden in cyclohexadepsipeptid-defizienten zellen
US6361973B1 (en) 1999-03-22 2002-03-26 Novozymes Biotech, Inc. Promoters for expressing genes in a fungal cell
WO2001055429A2 (en) 2000-01-24 2001-08-02 Polymun Scientific Immunbiologische Forschung Gmbh Method for the manufacture of recombinant trypsin
WO2002061064A2 (de) * 2001-02-01 2002-08-08 Roche Diagnostics Gmbh Verfahren zur herstellung von rekombinantem trypsin

Also Published As

Publication number Publication date
CN1694953B (zh) 2010-05-12
DK1546302T3 (da) 2009-12-14
EP1546302B1 (de) 2009-08-12
EP1546302A1 (de) 2005-06-29
CN1694953A (zh) 2005-11-09
US20040043455A1 (en) 2004-03-04
EP1546302A4 (de) 2006-08-16
WO2004020612A1 (en) 2004-03-11
AU2003273256A1 (en) 2004-03-19
DE60328802D1 (de) 2009-09-24

Similar Documents

Publication Publication Date Title
DE602004028724D1 (de) Verfahren zur herstellung sezernierter polypeptide
WO2000056900A3 (en) Promoter sequences derived from fusarium venenatum and uses thereof
ATE532870T1 (de) Verfahren zur herstellung von transglutaminase
DE69534822D1 (de) Verfahren zur bakteriellen herstellung von heterologen polypeptiden mit disulfidbrücke
JPH1014586A (ja) オリゴペプチド反復単位を有する大ポリペプチド
MXPA03010433A (es) Metodos para produccion recombinante de peptidos antifusogenicos.
JPH0829086B2 (ja) ポリクリングルプラスミノ−ゲン活性化因子
JP2004520049A5 (de)
ATE454039T1 (de) Verfharen zur herstellung von herpes-simplex- virus-amplikons, die resultierenden amplikons und ihre verwendung
ATE439432T1 (de) Verfahren zur herstellung von säugetier-trypsinen
IL150676A0 (en) NOVEL STRa6 POLYPEPTIDES
ATE480624T1 (de) Yapsin-mangelmutantenstamm von hansenula polymorpha sowie verfahren zur herstellung rekombinanter proteine damit
ATE383420T1 (de) Verwendung des cyp52a2a promotors für die erhöhung der genexpression in hefe
ATE408016T1 (de) Verfahren zur herstellung und sekretion modifizierter peptide
JPS60500043A (ja) 原核細胞又は真核細胞中で合成される融合蛋白質から成熟蛋白質を作る方法
EP1678308A4 (de) Expressionsvektor zur sekretion eines antikörperfragments unter verwendung einer e.coli-signalsequenz sowie verfahren zur massenproduktion des antikörperfragments
AU597317B2 (en) A method to export gene products to the growth medium of gram negative bacteria
DE602005017034D1 (de) Signalpeptid zur herstellung eines polypeptids
CN108103048B (zh) 一种低温基质金属蛋白酶及其编码基因与应用
CN104059900A (zh) 一种蛋白酶k及其应用
EP0718403B1 (de) Verfahren zur Herstellung von menschlichem oder humanem Matrilysin mittels rekombinanter DNA.
SE8802939D0 (sv) A method for the production and isolation of a fusion protein in eukaryotic cells
EP1262551A3 (de) Sorbitol-Dehydrogenase Gen, rekombinante DNA, und Verfahren zur Herstellung von Sorbitol-Dehydrogenase
EP0494149A1 (de) Modifizierte proteasen und deren verwendung in nahrungsmitteln
NZ515762A (en) Method of producing a heparin-binding protein by expressing it in a mammalian cell that can be cultured in anaerobic conditions

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties