ATE480624T1 - Yapsin-mangelmutantenstamm von hansenula polymorpha sowie verfahren zur herstellung rekombinanter proteine damit - Google Patents

Yapsin-mangelmutantenstamm von hansenula polymorpha sowie verfahren zur herstellung rekombinanter proteine damit

Info

Publication number
ATE480624T1
ATE480624T1 AT03736354T AT03736354T ATE480624T1 AT E480624 T1 ATE480624 T1 AT E480624T1 AT 03736354 T AT03736354 T AT 03736354T AT 03736354 T AT03736354 T AT 03736354T AT E480624 T1 ATE480624 T1 AT E480624T1
Authority
AT
Austria
Prior art keywords
polymorpha
mutant strain
yapsin
foreign protein
recombinant proteins
Prior art date
Application number
AT03736354T
Other languages
English (en)
Inventor
Hyun-Ah Kang
Sang-Ki Rhee
Min-Jeong Sohn
Jeong-Yoon Kim
Original Assignee
Korea Res Inst Of Bioscience
Leadbio Inc
Bioholdings Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Korea Res Inst Of Bioscience, Leadbio Inc, Bioholdings Co Ltd filed Critical Korea Res Inst Of Bioscience
Application granted granted Critical
Publication of ATE480624T1 publication Critical patent/ATE480624T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
AT03736354T 2002-06-29 2003-06-28 Yapsin-mangelmutantenstamm von hansenula polymorpha sowie verfahren zur herstellung rekombinanter proteine damit ATE480624T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20020037718 2002-06-29
PCT/KR2003/001279 WO2004003204A1 (en) 2002-06-29 2003-06-28 Hansenula polymorpha yapsin deficient mutant strain and process for the preparation of recombinant proteins using the same

Publications (1)

Publication Number Publication Date
ATE480624T1 true ATE480624T1 (de) 2010-09-15

Family

ID=29997410

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03736354T ATE480624T1 (de) 2002-06-29 2003-06-28 Yapsin-mangelmutantenstamm von hansenula polymorpha sowie verfahren zur herstellung rekombinanter proteine damit

Country Status (7)

Country Link
US (1) US7262287B2 (de)
EP (1) EP1534835B1 (de)
KR (1) KR100496758B1 (de)
AT (1) ATE480624T1 (de)
AU (1) AU2003237061A1 (de)
DE (1) DE60334109D1 (de)
WO (1) WO2004003204A1 (de)

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WO2004092408A2 (en) * 2003-04-18 2004-10-28 Warnex Research Inc. Oligonucleotides for the detection of salmonella species
CN1838968A (zh) 2003-08-08 2006-09-27 艾伯吉尼斯公司 针对甲状旁腺激素(pth)之抗体和其用途
US7318925B2 (en) 2003-08-08 2008-01-15 Amgen Fremont, Inc. Methods of use for antibodies against parathyroid hormone
KR100977446B1 (ko) * 2006-06-16 2010-08-24 한국생명공학연구원 분비 스트레스 반응을 조절하는 한세눌라 폴리모르파의신규한 유전자 및 상기 유전자를 이용하여 재조합 단백질의분비 발현 효율을 증가시키는 방법
CA2887752C (en) 2007-04-03 2020-03-24 Vib Vzw Glycosylation of molecules
US9120871B2 (en) * 2009-01-23 2015-09-01 Novo Nordisk A/S Process for preparing FGF21 with low degree of O-glycosylation
KR102000383B1 (ko) 2009-09-29 2019-07-15 유니버시테이트 젠트 만노스-1-포스포-6-만노스 결합의 포스포-6-만노스로의 가수분해
WO2011061629A2 (en) 2009-11-19 2011-05-26 Oxyrane Uk Limited Yeast strains producing mammalian-like complex n-glycans
ES2550761T3 (es) 2009-11-25 2015-11-12 Novo Nordisk A/S Método para producción de polipéptidos
US9347050B2 (en) 2010-09-29 2016-05-24 Oxyrane Uk Limited Mannosidases capable of uncapping mannose-1-phospho-6-mannose linkages and demannosylating phosphorylated N-glycans and methods of facilitating mammalian cellular uptake of glycoproteins
CA2812872C (en) 2010-09-29 2021-08-03 Oxyrane Uk Limited De-mannosylation of phosphorylated n-glycans
EP3628326B1 (de) 2012-03-15 2024-02-28 Oxyrane UK Limited Verfahren und materialien zur behandlung von morbus pompe
CN111315763B (zh) * 2017-10-03 2025-05-09 保尔特纺织品公司 用于生产重组丝的经修饰菌株
US10647975B2 (en) 2017-10-03 2020-05-12 Bolt Threads, Inc. Modified strains for the production of recombinant silk
CN119081906B (zh) * 2024-10-16 2026-01-06 广东丸美生物技术股份有限公司 一种可稳定表达重组胶原蛋白的毕赤酵母菌株及其应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0336056B1 (de) * 1988-01-27 1995-04-05 M & D RESEARCH CO., LTD. Protease
US5612198A (en) * 1990-09-04 1997-03-18 The Salk Institute Production of insulin-like growth factor-1 in methylotrophic yeast cells
JPH07108232B2 (ja) * 1990-10-09 1995-11-22 エム・ディ・リサーチ株式会社 ペプチド又は蛋白質の製造方法
DK82893D0 (da) * 1993-07-08 1993-07-08 Novo Nordisk As Peptid
GB9404270D0 (en) * 1994-03-05 1994-04-20 Delta Biotechnology Ltd Yeast strains and modified albumins
US6110703A (en) * 1996-07-05 2000-08-29 Novo Nordisk A/S Method for the production of polypeptides
CA2258307C (en) 1996-07-05 2008-02-12 Novo Nordisk A/S Method of producing heterologous short chain polypeptides by yeast with impaired yap3 protease
KR100246932B1 (ko) * 1997-09-12 2000-03-15 박호군 Yap3 유전자가 결손된 신규한 효모균주 및 그를 이용한 재조합 인체 부갑상선호르몬의 생산
EP1080207A4 (de) * 1999-03-04 2004-08-25 Korea Res Inst Chem Tech Hansenula polymorpha mutanten und verfahren zur herstellung rekombinanter proteine mittels dieser
KR100441384B1 (ko) 2002-01-08 2004-07-21 주식회사 안지오랩 인간 혈청 알부민-팀프-2 융합 단백질 발현시스템 및재조합 인간 혈청 알부민-팀프-2 융합 단백질
KR102008884B1 (ko) 2014-01-23 2019-08-09 동우 화인켐 주식회사 실리콘계 화합물막 식각액 조성물
KR102001775B1 (ko) 2018-04-10 2019-07-18 조성은 Led 렌즈 및 이를 포함한 led 패키지

Also Published As

Publication number Publication date
KR20040002806A (ko) 2004-01-07
EP1534835A1 (de) 2005-06-01
US7262287B2 (en) 2007-08-28
US20050239074A1 (en) 2005-10-27
WO2004003204A1 (en) 2004-01-08
KR100496758B1 (ko) 2005-06-22
AU2003237061A1 (en) 2004-01-19
EP1534835A4 (de) 2005-12-14
DE60334109D1 (de) 2010-10-21
AU2003237061A8 (en) 2004-01-19
EP1534835B1 (de) 2010-09-08

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