ATE442458T1 - Vorrichtung und verfahren zur quantifizierung von mrna mit hohem durchlauf aus vollblut - Google Patents

Vorrichtung und verfahren zur quantifizierung von mrna mit hohem durchlauf aus vollblut

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Publication number
ATE442458T1
ATE442458T1 AT08075176T AT08075176T ATE442458T1 AT E442458 T1 ATE442458 T1 AT E442458T1 AT 08075176 T AT08075176 T AT 08075176T AT 08075176 T AT08075176 T AT 08075176T AT E442458 T1 ATE442458 T1 AT E442458T1
Authority
AT
Austria
Prior art keywords
mrna
whole blood
high flow
quantifying high
quantifying
Prior art date
Application number
AT08075176T
Other languages
English (en)
Inventor
Masato Mitsuhashi
Original Assignee
Hitachi Chemical Co Ltd
Hitachi Chemical Res Ct Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hitachi Chemical Co Ltd, Hitachi Chemical Res Ct Inc filed Critical Hitachi Chemical Co Ltd
Application granted granted Critical
Publication of ATE442458T1 publication Critical patent/ATE442458T1/de

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06Lysis of microorganisms
    • C12N1/066Lysis of microorganisms by physical processes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6846Common amplification features

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT08075176T 2003-10-30 2004-10-29 Vorrichtung und verfahren zur quantifizierung von mrna mit hohem durchlauf aus vollblut ATE442458T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US69896703A 2003-10-30 2003-10-30
US10/796,298 US7745180B2 (en) 2002-04-24 2004-03-09 Device and method for high-throughput quantification of mRNA from whole blood

Publications (1)

Publication Number Publication Date
ATE442458T1 true ATE442458T1 (de) 2009-09-15

Family

ID=34556635

Family Applications (4)

Application Number Title Priority Date Filing Date
AT08014199T ATE517193T1 (de) 2003-10-30 2004-10-29 Vorrichtung und verfahren zur quantifizierung von mrna mit hohem durchlauf aus vollblut
AT04796890T ATE491042T1 (de) 2003-10-30 2004-10-29 Vorrichtung und verfahren zur quantifizierung von mrna aus vollblut mit hohem durchsatz
AT08014198T ATE466110T1 (de) 2003-10-30 2004-10-29 Vorrichtung und verfahren zur quantifizierung von mrna mit hohem durchlauf aus vollblut
AT08075176T ATE442458T1 (de) 2003-10-30 2004-10-29 Vorrichtung und verfahren zur quantifizierung von mrna mit hohem durchlauf aus vollblut

Family Applications Before (3)

Application Number Title Priority Date Filing Date
AT08014199T ATE517193T1 (de) 2003-10-30 2004-10-29 Vorrichtung und verfahren zur quantifizierung von mrna mit hohem durchlauf aus vollblut
AT04796890T ATE491042T1 (de) 2003-10-30 2004-10-29 Vorrichtung und verfahren zur quantifizierung von mrna aus vollblut mit hohem durchsatz
AT08014198T ATE466110T1 (de) 2003-10-30 2004-10-29 Vorrichtung und verfahren zur quantifizierung von mrna mit hohem durchlauf aus vollblut

Country Status (7)

Country Link
US (6) US7745180B2 (de)
EP (4) EP1682678B1 (de)
JP (3) JP4594321B2 (de)
CN (4) CN1997752A (de)
AT (4) ATE517193T1 (de)
DE (3) DE602004023143D1 (de)
WO (1) WO2005042784A2 (de)

Families Citing this family (69)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6844158B1 (en) * 1997-12-22 2005-01-18 Hitachi Chemical Co., Ltd. Direct RT-PCR on oligonucleotide-immobilized PCR microplates
SE9904539D0 (sv) * 1999-12-10 1999-12-10 Alphahelix Ab Method and device for the handling of samples and reagents
ATE480618T1 (de) * 2000-10-31 2010-09-15 Hitachi Chemical Res Ct Inc Methode für das sammeln und das verwenden von kern-mrna
WO2002042500A2 (en) * 2000-10-31 2002-05-30 Hitachi Chemical Research Center, Inc. Apparatus and method for electrophoretic microspot concentration
US7745180B2 (en) * 2002-04-24 2010-06-29 Hitachi Chemical Co., Ltd. Device and method for high-throughput quantification of mRNA from whole blood
JP2008503206A (ja) * 2004-05-25 2008-02-07 日立化成工業株式会社 癌感受性を測定する方法
US20050277121A1 (en) 2004-06-11 2005-12-15 Ambion, Inc. Crude biological derivatives competent for nucleic acid detection
JP2006083114A (ja) * 2004-09-17 2006-03-30 Hitachi High-Technologies Corp 核酸抽出方法、及び核酸遊離方法
CN101044249B (zh) * 2004-10-20 2012-09-05 日立化成工业株式会社 通过定量mRNA定制给药的方法
AU2006204858A1 (en) * 2005-01-13 2006-07-20 Perkinelmer Health Sciences, Inc. Microfluidic rare cell detection device
ATE512234T1 (de) * 2005-04-28 2011-06-15 Hitachi Chemical Res Ct Inc Ex-vivo-genexpression in vollblut als modell für die beurteilung von individueller variation gegenüber nahrungszusätzen
JP4800384B2 (ja) * 2005-06-08 2011-10-26 ヒタチ ケミカル リサーチ センター インコーポレイテッド 腫瘍細胞及び刺激された白血球におけるmRNAの発現プロファイルに基づく腫瘍性疾患に対する免疫応答を予測する方法
US7569367B2 (en) 2005-09-07 2009-08-04 Roche Diagnostics Operations, Inc. Nucleic acid preparation from whole blood for use in diagnosis of transmissible spongiform encephalopathy
WO2007100986A2 (en) * 2006-02-24 2007-09-07 Rosetta Inpharmatics Llc Extraction and diagnostic fluid devices, systems and methods of use
US20080075789A1 (en) * 2006-02-28 2008-03-27 The Regents Of The University Of California Genes differentially expressed in bipolar disorder and/or schizophrenia
ATE518011T1 (de) * 2006-04-07 2011-08-15 Hitachi Chemical Co Ltd Erhöhte expression von t- zellenrezeptorvermittelter tumornekrosefaktorsuperfamilie und chemokin-mrna in peripheren blut leukozyten bei patienten mit morbus& xa;crohn
JP2009536021A (ja) * 2006-04-07 2009-10-08 日立化成工業株式会社 関節リウマチの患者の末梢血白血球におけるFC受容体媒介性腫瘍壊死因子スーパーファミリ及びケモカインのmRNA発現の増大
US20090298071A1 (en) * 2006-05-08 2009-12-03 Masato Mitsuhashi Method for testing drug sensitivity in solid tumors by quantifying mrna expression in thinly-sliced tumor tissue
EP2027293B1 (de) * 2006-05-24 2014-11-19 Gen-Probe Incorporated Verfahren zum lysieren von mycobacterium und amplifikation seiner nukleinsäuren
US20080153078A1 (en) * 2006-06-15 2008-06-26 Braman Jeffrey C System for isolating biomolecules from a sample
US8084443B2 (en) 2007-10-01 2011-12-27 Longhorn Vaccines & Diagnostics Llc Biological specimen collection and transport system and methods of use
US8080645B2 (en) * 2007-10-01 2011-12-20 Longhorn Vaccines & Diagnostics Llc Biological specimen collection/transport compositions and methods
US8097419B2 (en) 2006-09-12 2012-01-17 Longhorn Vaccines & Diagnostics Llc Compositions and method for rapid, real-time detection of influenza A virus (H1N1) swine 2009
US9481912B2 (en) 2006-09-12 2016-11-01 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for detecting and identifying nucleic acid sequences in biological samples
WO2008116150A2 (en) 2007-03-21 2008-09-25 Cedars-Sinai Medical Center Ileal pouch-anal anastomosis (ipaa) factors in the treatment of inflammatory bowel disease
US11041215B2 (en) 2007-08-24 2021-06-22 Longhorn Vaccines And Diagnostics, Llc PCR ready compositions and methods for detecting and identifying nucleic acid sequences
US9683256B2 (en) 2007-10-01 2017-06-20 Longhorn Vaccines And Diagnostics, Llc Biological specimen collection and transport system
EP2772267B1 (de) 2007-08-27 2016-04-27 Longhorn Vaccines and Diagnostics, LLC Immunogene Zusammensetzungen und Verfahren
US10004799B2 (en) 2007-08-27 2018-06-26 Longhorn Vaccines And Diagnostics, Llc Composite antigenic sequences and vaccines
US11041216B2 (en) 2007-10-01 2021-06-22 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for detecting and quantifying nucleic acid sequences in blood samples
JP2011502535A (ja) * 2007-11-14 2011-01-27 日立化成工業株式会社 末梢血白血球における、Fc受容体を介した腫瘍壊死因子スーパーファミリーmRNA発現
US20090215064A1 (en) * 2008-02-27 2009-08-27 Hitachi Chemical Co., Ltd. Quantitative assessment of individual cancer susceptibility by measuring dna damage-induced mrna in whole blood
US8357672B2 (en) * 2008-06-13 2013-01-22 Bio-Rad Laboratories, Inc. Cell lysis reagent for isolation of RNA
US20110229471A1 (en) 2008-11-26 2011-09-22 Cedars-Sinai Medical Center Methods of determining responsiveness to anti-tnf alpha therapy in inflammatory bowel disease
US9580752B2 (en) 2008-12-24 2017-02-28 Cedars-Sinai Medical Center Methods of predicting medically refractive ulcerative colitis (MR-UC) requiring colectomy
WO2011004517A1 (ja) * 2009-07-06 2011-01-13 オリンパス株式会社 標的核酸の検出方法、及び大腸癌の検査方法
WO2011156734A2 (en) * 2010-06-11 2011-12-15 Hitachi Chemical Co., Ltd. Method of characterizing vascular diseases
WO2011156763A1 (en) 2010-06-11 2011-12-15 Hitachi Chemical Co., Ltd. Methods for characterizing kidney function
US8721413B2 (en) * 2010-10-06 2014-05-13 Jameel Akhtab Mohammed Computer game with a target number of formations created from falling pieces in a triangular grid
JP5823031B2 (ja) 2011-06-10 2015-11-25 日立化成株式会社 小胞捕捉デバイスおよびそれを用いるための方法
EP2734233B1 (de) 2011-07-18 2017-11-01 Hitachi Chemical Co., Ltd. Verfahren zur vorhersage des ansprechens eines wirtes auf krebsimmuntherapien durch ex-vivo-induktion von mit leukozytenfunktionen assoziierten mrnas
WO2013101741A1 (en) 2011-12-30 2013-07-04 Abbott Molecular, Inc. Channels with cross-sectional thermal gradients
US20130172208A1 (en) * 2012-01-04 2013-07-04 Hitachi Chemical Research Center, Inc. ASSESSMENT OF BONE MARROW RECOVERY BY MEASURING PLASMA EXOSOME mRNAS
EP3494989B1 (de) 2012-01-26 2025-07-16 Longhorn Vaccines and Diagnostics, LLC Zusammengesetzte antigensequenzen und impfstoffe
KR20230109779A (ko) 2013-03-27 2023-07-20 세다르스-신나이 메디칼 센터 Tl1a 기능 및 관련된 신호전달 경로의 저해에 의한섬유증 및 염증의 완화 및 반전
WO2014182330A1 (en) * 2013-05-06 2014-11-13 Hitachi Chemical Company Ltd Devices and methods for capturing target molecules
EP3022295A4 (de) 2013-07-19 2017-03-01 Cedars-Sinai Medical Center Signatur des tl1a (tnfsf15)-signalweges
CN103645332B (zh) * 2013-12-19 2015-04-29 南京卡迪奥密生物技术有限公司 自动化高通量检测胆固醇以及其它分析物细胞流出的多功能工作站
JP6113674B2 (ja) * 2014-02-13 2017-04-12 株式会社神戸製鋼所 耐熱性に優れる表面被覆層付き銅合金板条
USD746467S1 (en) * 2014-04-04 2015-12-29 Hitachi Chemical Company, Ltd. Filter sheet for a cell collecting cartridge
USD748801S1 (en) * 2014-04-04 2016-02-02 Hitachi Chemical Company, Ltd. Filter sheet for a cell collecting cartridge
USD748267S1 (en) * 2014-04-04 2016-01-26 Hitachi Chemical Company, Ltd. Filter sheet for a cell collecting cartridge
USD750785S1 (en) * 2014-04-04 2016-03-01 Hitachi Chemical Company, Ltd. Filter sheet for a cell collecting cartridge
USD747487S1 (en) * 2014-04-04 2016-01-12 Hitachi Chemical Company, Ltd. Filter sheet for a cell collecting cartridge
US10266895B2 (en) 2014-11-05 2019-04-23 Hitachi Chemical Company Ltd. Exosomes and microvesicles in intestinal luminal fluids and stool and use of same for the assessment of inflammatory bowel disease
EP3218521B1 (de) 2014-11-12 2019-12-25 Hitachi Chemical Co., Ltd. Verfahren zur diagnose von organverletzung
JP6820865B2 (ja) * 2015-04-29 2021-01-27 ペルキネルマー ヘルス サイエンシーズ, インコーポレイテッド 試料収集および送達装置
EP3294448A4 (de) 2015-05-14 2018-12-12 Longhorn Vaccines and Diagnostics, LLC Schnellverfahren zur extraktion von nukleinsäuren aus biologischen proben
DE112016003948T5 (de) 2015-08-31 2018-05-09 City Of Sapporo Molekulare verfahren zum beurteilen einer urothelialen erkrankung
KR20240095481A (ko) 2016-03-17 2024-06-25 세다르스-신나이 메디칼 센터 Rnaset2를 통한 염증성 장 질환의 진단 방법
CN106190817B (zh) * 2016-07-19 2019-01-08 温州市高亿诺生物科技有限公司 基于多孔板的核酸裂解液分离方法和装置
KR20180099964A (ko) * 2017-02-27 2018-09-06 (주)오상헬스케어 혈액 분석용 스트립
US20190283022A1 (en) * 2018-03-15 2019-09-19 Behrad Vahidi Highly parallel microfluidic blood separation device
AU2020220357B2 (en) * 2019-02-11 2025-06-26 Ethris Gmbh mRNA purification by tangential flow filtration
EP3999624B1 (de) 2019-07-16 2026-03-25 Meliolabs Inc. Verfahren und vorrichtungen für einzellenbasierte digitale hochauflösende schmelzanalyse
CN110452998A (zh) * 2019-09-17 2019-11-15 浙江海洋大学 利用线粒体dna控制区进行黄鲫遗传学分析的方法
CN110724633A (zh) * 2019-11-11 2020-01-24 浙江汇泽医药科技有限公司 一种微量细胞核酸提取与扩增系统及工艺
KR102375663B1 (ko) * 2020-03-24 2022-03-16 재단법인대구경북과학기술원 무손실 세포 염색 방법 및 장치
CN116529389A (zh) * 2020-08-20 2023-08-01 贝克顿迪金森公司 用于从血培养物中分离细菌的血细胞裂解剂

Family Cites Families (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4193980A (en) 1978-01-05 1980-03-18 Corning Glass Works Dry preparation for reticulocyte staining
US4652525A (en) * 1978-04-19 1987-03-24 The Regents Of The University Of California Recombinant bacterial plasmids containing the coding sequences of insulin genes
US5175109A (en) 1986-09-10 1992-12-29 Toa Medical Electronics Co., Ltd. Reagent for classifying leukocytes by flow cytometry
US4925572A (en) 1987-10-20 1990-05-15 Pall Corporation Device and method for depletion of the leukocyte content of blood and blood components
US4923620A (en) * 1987-10-20 1990-05-08 Pall Corporation Device for depletion of the leukocyte content of blood and blood components
US4880548A (en) 1988-02-17 1989-11-14 Pall Corporation Device and method for separating leucocytes from platelet concentrate
US4958013A (en) * 1989-06-06 1990-09-18 Northwestern University Cholesteryl modified oligonucleotides
US5045449A (en) * 1989-06-14 1991-09-03 Thomas Jefferson University Methods of detecting a genetic predisposition for vascular aneurysms
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
GB9027055D0 (en) * 1990-12-13 1991-02-06 Sandoz Ltd Organic compounds
US6300058B1 (en) 1992-01-29 2001-10-09 Hitachi Chemical Research Center, Inc. Method for measuring messenger RNA
IL104670A (en) * 1993-02-09 1998-04-05 Travenol Lab Israel Ltd Leukocyte removal method and filter unit for same
GB9314249D0 (en) 1993-07-09 1993-08-18 Proofname Ltd Purification method and apparatus
NO180658C (no) * 1994-03-10 1997-05-21 Oeystein Fodstad Fremgangsmåte og anordning for deteksjon av spesifikke målceller i spesialiserte eller blandede cellepopulasjoner og opplösninger som inneholder blandede cellepopulasjoner
US5491063A (en) 1994-09-01 1996-02-13 Hoffmann-La Roche Inc. Methods for in-solution quenching of fluorescently labeled oligonucleotide probes
US5691132A (en) * 1994-11-14 1997-11-25 Cerus Corporation Method for inactivating pathogens in red cell compositions using quinacrine mustard
US5801064A (en) * 1995-12-04 1998-09-01 Foresman; Mark D. Assay methods and reagents for detecting autoantibodies
US6042859A (en) 1996-04-18 2000-03-28 Ramot University Authority For Applied Research And Industrial Development Ltd. Method for the long-term preservation of meat
JP3249919B2 (ja) 1996-07-30 2002-01-28 株式会社堀場製作所 免疫測定方法
US5976797A (en) 1996-12-20 1999-11-02 Hitachi Chemical Co., Ltd. Method for quantifying total mRNA with poly(A)-complementary oligonucleotide-immobilized microtiter plate
US5948666A (en) * 1997-08-06 1999-09-07 Diversa Corporation Isolation and identification of polymerases
EP1029918A4 (de) 1997-09-26 2003-01-02 Kyowa Hakko Kogyo Kk Rezeptor von tötenden t-zellen, die den menschlichen immunodefizienz virus erkennen
DE69839734D1 (de) 1997-12-22 2008-08-28 Hitachi Chemical Co Ltd Direkte rt-pcr auf mikrotiterplatten mit immobilisierten oligonukleotid
US6844158B1 (en) 1997-12-22 2005-01-18 Hitachi Chemical Co., Ltd. Direct RT-PCR on oligonucleotide-immobilized PCR microplates
EP1119576B1 (de) 1998-10-09 2003-06-11 Whatman Bioscience Limited Verfahren zur isolierung von nukleinsäure und vorrichtung hierfür
DE19914966A1 (de) * 1999-04-01 2000-10-05 Basf Ag Verfahren zur kontinuierlich betriebenen destillativen Abtrennung eines höherschmelzenden Stoffes
WO2000077253A1 (en) 1999-06-16 2000-12-21 Hitachi, Ltd. Apparatus and method for gene examination
JP3707303B2 (ja) 1999-06-30 2005-10-19 株式会社日立製作所 ヒスタミン計測方法およびヒスタミン計測装置
IL151872A0 (en) * 2000-03-24 2003-04-10 Micromet Ag mRNA AMPLIFICATION
EP1138780A1 (de) 2000-03-31 2001-10-04 Boehringer Mannheim Gmbh Detektion ausgesäter Tumorzellen mittels quantitativer RT-PCR auf Cytokeratin-20 mRNA
AU2001263836A1 (en) * 2000-04-11 2001-10-23 Bureco Ag Method of nucleic acid recovery
EP1337670A4 (de) 2000-08-16 2004-07-14 Whatman Inc Transfusionsmedizinfiltriermittel zur abreicherung von leukozyten als quelle für genetisches material für genotypisierungsstudien
AU2001288853A1 (en) * 2000-09-07 2002-03-22 The Brigham And Women's Hospital, Inc. Methods of detecting cancer based on prostasin
US6602718B1 (en) * 2000-11-08 2003-08-05 Becton, Dickinson And Company Method and device for collecting and stabilizing a biological sample
CA2429722A1 (en) 2000-11-28 2002-06-06 Wyeth Expression analysis of fkbp nucleic acids and polypeptides useful in the diagnosis and treatment of prostate cancer
US6638722B2 (en) 2001-06-13 2003-10-28 Invitrogen Corporation Method for rapid amplification of DNA
CN1617938A (zh) 2002-01-16 2005-05-18 戴诺生物技术有限公司 从单个样品中分离核酸和蛋白质的方法
US7745180B2 (en) 2002-04-24 2010-06-29 Hitachi Chemical Co., Ltd. Device and method for high-throughput quantification of mRNA from whole blood
CN1188529C (zh) * 2002-06-11 2005-02-09 刘全俊 一种基因芯片的纳米金标记银染检测法
US20050059024A1 (en) * 2003-07-25 2005-03-17 Ambion, Inc. Methods and compositions for isolating small RNA molecules

Also Published As

Publication number Publication date
EP2058405A1 (de) 2009-05-13
JP5160591B2 (ja) 2013-03-13
EP1953245B1 (de) 2009-09-09
CN101696448A (zh) 2010-04-21
DE602004023143D1 (de) 2009-10-22
US20070224632A1 (en) 2007-09-27
EP1682678A2 (de) 2006-07-26
US20060199212A1 (en) 2006-09-07
CN101696442B (zh) 2013-04-17
US8101344B2 (en) 2012-01-24
DE602004026947D1 (de) 2010-06-10
US8076105B2 (en) 2011-12-13
EP2058405B1 (de) 2011-07-20
US7745180B2 (en) 2010-06-29
CN101696409B (zh) 2012-06-27
CN101696409A (zh) 2010-04-21
EP1997912A2 (de) 2008-12-03
JP5160592B2 (ja) 2013-03-13
WO2005042784A3 (en) 2005-10-13
EP1997912B1 (de) 2010-04-28
EP1953245A1 (de) 2008-08-06
EP1997912A3 (de) 2008-12-10
DE602004030472D1 (de) 2011-01-20
ATE466110T1 (de) 2010-05-15
US7981608B2 (en) 2011-07-19
US7939300B2 (en) 2011-05-10
JP4594321B2 (ja) 2010-12-08
JP2010162050A (ja) 2010-07-29
JP2007509633A (ja) 2007-04-19
CN101696442A (zh) 2010-04-21
US20080009009A1 (en) 2008-01-10
ATE517193T1 (de) 2011-08-15
CN1997752A (zh) 2007-07-11
US20070224631A1 (en) 2007-09-27
JP2010220619A (ja) 2010-10-07
ATE491042T1 (de) 2010-12-15
US7968288B1 (en) 2011-06-28
US20040265864A1 (en) 2004-12-30
WO2005042784A2 (en) 2005-05-12
EP1682678B1 (de) 2010-12-08

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