ATE480618T1 - Methode für das sammeln und das verwenden von kern-mrna - Google Patents
Methode für das sammeln und das verwenden von kern-mrnaInfo
- Publication number
- ATE480618T1 ATE480618T1 AT01270141T AT01270141T ATE480618T1 AT E480618 T1 ATE480618 T1 AT E480618T1 AT 01270141 T AT01270141 T AT 01270141T AT 01270141 T AT01270141 T AT 01270141T AT E480618 T1 ATE480618 T1 AT E480618T1
- Authority
- AT
- Austria
- Prior art keywords
- mrna
- cell
- collecting
- solution
- novel method
- Prior art date
Links
- 108020004999 messenger RNA Proteins 0.000 title abstract 4
- 210000004027 cell Anatomy 0.000 abstract 2
- 210000000170 cell membrane Anatomy 0.000 abstract 2
- 210000003855 cell nucleus Anatomy 0.000 abstract 1
- 210000000805 cytoplasm Anatomy 0.000 abstract 1
- 239000012528 membrane Substances 0.000 abstract 1
- 210000004940 nucleus Anatomy 0.000 abstract 1
- 230000035699 permeability Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US24467200P | 2000-10-31 | 2000-10-31 | |
| US30803801P | 2001-07-26 | 2001-07-26 | |
| PCT/US2001/049498 WO2002066637A2 (en) | 2000-10-31 | 2001-10-25 | Method for collecting and using nuclear mrna |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE480618T1 true ATE480618T1 (de) | 2010-09-15 |
Family
ID=26936712
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT01270141T ATE480618T1 (de) | 2000-10-31 | 2001-10-25 | Methode für das sammeln und das verwenden von kern-mrna |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US7374881B2 (de) |
| EP (1) | EP1330518B1 (de) |
| JP (1) | JP5416326B2 (de) |
| CN (1) | CN1294262C (de) |
| AT (1) | ATE480618T1 (de) |
| DE (1) | DE60143046D1 (de) |
| WO (1) | WO2002066637A2 (de) |
Families Citing this family (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6844158B1 (en) * | 1997-12-22 | 2005-01-18 | Hitachi Chemical Co., Ltd. | Direct RT-PCR on oligonucleotide-immobilized PCR microplates |
| WO2002042500A2 (en) | 2000-10-31 | 2002-05-30 | Hitachi Chemical Research Center, Inc. | Apparatus and method for electrophoretic microspot concentration |
| US20050277121A1 (en) | 2004-06-11 | 2005-12-15 | Ambion, Inc. | Crude biological derivatives competent for nucleic acid detection |
| ATE512234T1 (de) * | 2005-04-28 | 2011-06-15 | Hitachi Chemical Res Ct Inc | Ex-vivo-genexpression in vollblut als modell für die beurteilung von individueller variation gegenüber nahrungszusätzen |
| GB0522193D0 (en) | 2005-10-31 | 2005-12-07 | Axis Shield Asa | Method |
| ATE518011T1 (de) * | 2006-04-07 | 2011-08-15 | Hitachi Chemical Co Ltd | Erhöhte expression von t- zellenrezeptorvermittelter tumornekrosefaktorsuperfamilie und chemokin-mrna in peripheren blut leukozyten bei patienten mit morbus& xa;crohn |
| JP2009536021A (ja) * | 2006-04-07 | 2009-10-08 | 日立化成工業株式会社 | 関節リウマチの患者の末梢血白血球におけるＦＣ受容体媒介性腫瘍壊死因子スーパーファミリ及びケモカインのｍＲＮＡ発現の増大 |
| JP2011502535A (ja) | 2007-11-14 | 2011-01-27 | 日立化成工業株式会社 | 末梢血白血球における、Ｆｃ受容体を介した腫瘍壊死因子スーパーファミリーｍＲＮＡ発現 |
| US8546127B2 (en) * | 2008-06-30 | 2013-10-01 | General Electric Company | Bacteria/RNA extraction device |
| US8304185B2 (en) | 2009-07-17 | 2012-11-06 | Canon U.S. Life Sciences, Inc. | Methods and systems for DNA isolation on a microfluidic device |
| US20100062946A1 (en) * | 2008-09-08 | 2010-03-11 | Lis John T | Genome-wide method for mapping of engaged rna polymerases quantitatively and at high resolution |
| CN101497925B (zh) * | 2008-12-17 | 2012-05-02 | 安徽医科大学 | Leber氏遗传性视神经病变相关mtDNA突变位点集成检测基因芯片及其制备与应用 |
| JP5729904B2 (ja) * | 2009-06-02 | 2015-06-03 | キヤノン株式会社 | 細胞から蛋白質、ｄｎａ、ｒｎａを調製する方法 |
| EP2454378B1 (de) * | 2009-07-17 | 2015-12-23 | Canon U.S. Life Sciences, Inc. | Verfahren und systeme für dna-isolation bei einer mikrofluidischen vorrichtung |
| MX2012001236A (es) * | 2009-08-04 | 2012-03-16 | Du Pont | Proceso y dispositivo para recolectar acidos nucleicos de microorganismos de una muestra en particulas. |
| WO2011068088A1 (ja) | 2009-12-04 | 2011-06-09 | 株式会社日立製作所 | ２次元cDNAライブラリを用いた遺伝子発現解析方法 |
| WO2011156763A1 (en) | 2010-06-11 | 2011-12-15 | Hitachi Chemical Co., Ltd. | Methods for characterizing kidney function |
| WO2011156734A2 (en) * | 2010-06-11 | 2011-12-15 | Hitachi Chemical Co., Ltd. | Method of characterizing vascular diseases |
| US20110312537A1 (en) * | 2010-06-17 | 2011-12-22 | Geneasys Pty Ltd | Loc device for amplifying and detecting target nucleic acid sequences using electrochemiluminescent resonant energy transfer, linear probes with covalently attached primers |
| JP5823031B2 (ja) | 2011-06-10 | 2015-11-25 | 日立化成株式会社 | 小胞捕捉デバイスおよびそれを用いるための方法 |
| WO2014182330A1 (en) | 2013-05-06 | 2014-11-13 | Hitachi Chemical Company Ltd | Devices and methods for capturing target molecules |
| US10266895B2 (en) | 2014-11-05 | 2019-04-23 | Hitachi Chemical Company Ltd. | Exosomes and microvesicles in intestinal luminal fluids and stool and use of same for the assessment of inflammatory bowel disease |
| EP3218521B1 (de) | 2014-11-12 | 2019-12-25 | Hitachi Chemical Co., Ltd. | Verfahren zur diagnose von organverletzung |
| DE112016003948T5 (de) | 2015-08-31 | 2018-05-09 | City Of Sapporo | Molekulare verfahren zum beurteilen einer urothelialen erkrankung |
| US11293049B2 (en) * | 2016-01-08 | 2022-04-05 | Pathoquest | Modulation of accessibility of host nucleic acids to nucleic acid digesting enzymes in acellular biological fluids |
| CN115247204A (zh) * | 2022-02-15 | 2022-10-28 | 温州医科大学 | 一种基于qRT-PCR技术检测lncRNA亚细胞定位的方法 |
| CN115508269A (zh) * | 2022-10-20 | 2022-12-23 | 上海华简检测技术有限公司 | 一种细胞处理剂定量取样对比分析法 |
Family Cites Families (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4139346A (en) * | 1977-11-28 | 1979-02-13 | Enzo Bio Chem Incorporated | Nucleic acid and protein binding paper |
| US4906561A (en) | 1981-09-14 | 1990-03-06 | Thornthwaite Jerry T | Nuclear isolation medium and procedure for separating cell nuclei |
| US4430278A (en) | 1982-02-01 | 1984-02-07 | Jones Sr John L | Process for increasing the permeability of plastic membrane |
| US5057438A (en) | 1986-09-24 | 1991-10-15 | Agency Of Industrial Science And Technology | Electrophoretic antigen-antibody determination with laminate of multiple membranes |
| DE3715856A1 (de) | 1987-05-12 | 1988-12-01 | Biometra Biomedizinische Analy | Vorrichtung zum konzentrieren von in einer fluessigkeit befindlichen, elektrisch geladenen - insbesondere aus einem gel eluierten - makromolekuelen |
| JPH01179090A (ja) * | 1988-01-06 | 1989-07-17 | Yamaha Corp | 自動伴奏装置 |
| US5158661A (en) | 1991-03-22 | 1992-10-27 | Fotodyne Incorporated | Electrophoresis temperature control apparatus |
| DE69223617T2 (de) * | 1992-09-18 | 1998-04-09 | Amersham Int Plc | Verfahren zum Auffangen von Zellkernen und dafür verwendete Vorrichtung |
| US5955272A (en) * | 1993-02-26 | 1999-09-21 | University Of Massachusetts | Detection of individual gene transcription and splicing |
| US6113763A (en) | 1996-11-04 | 2000-09-05 | Board Of Trustee Operating Michigan State University | Method for measuring cellular chemical profiles |
| EP0942969A1 (de) | 1996-11-12 | 1999-09-22 | Qbi Enterprises Ltd. | Verfahren zur identifizierung von genen welche auf der stufe der translation reguliert werden |
| JP2001506005A (ja) * | 1996-12-11 | 2001-05-08 | ナイコメッド・アマーシャム・ピーエルシー | 細胞の選択的溶解 |
| US5964997A (en) | 1997-03-21 | 1999-10-12 | Sarnoff Corporation | Balanced asymmetric electronic pulse patterns for operating electrode-based pumps |
| JP2003144149A (ja) * | 1997-03-31 | 2003-05-20 | Takara Holdings Inc | 分離装置及びその使用方法 |
| DE19725190A1 (de) | 1997-06-14 | 1998-12-17 | Innova Gmbh | Vorrichtungen mit integrierten Elektroden aus elektrisch leitfähigen Kunststoffen |
| US5990298A (en) * | 1997-09-12 | 1999-11-23 | The University Of Connecticut | CIS-acting cellular nucleic acid molecules |
| DE69839734D1 (de) | 1997-12-22 | 2008-08-28 | Hitachi Chemical Co Ltd | Direkte rt-pcr auf mikrotiterplatten mit immobilisierten oligonukleotid |
| US6844158B1 (en) | 1997-12-22 | 2005-01-18 | Hitachi Chemical Co., Ltd. | Direct RT-PCR on oligonucleotide-immobilized PCR microplates |
| EP1075543A2 (de) * | 1998-04-27 | 2001-02-14 | Sidney Kimmel Cancer Center | Zielnukleinsäure mit reduzierter komplexität und verfahren zu ihrer verwendung |
| EP1119576B1 (de) * | 1998-10-09 | 2003-06-11 | Whatman Bioscience Limited | Verfahren zur isolierung von nukleinsäure und vorrichtung hierfür |
| US6287440B1 (en) | 1999-06-18 | 2001-09-11 | Sandia Corporation | Method for eliminating gas blocking in electrokinetic pumping systems |
| US6878540B2 (en) * | 1999-06-25 | 2005-04-12 | Cepheid | Device for lysing cells, spores, or microorganisms |
| US6319379B1 (en) | 1999-08-23 | 2001-11-20 | The Regents Of The University Of California | Modified electrokinetic sample injection method in chromatography and electrophoresis analysis |
| US6432290B1 (en) | 1999-11-26 | 2002-08-13 | The Governors Of The University Of Alberta | Apparatus and method for trapping bead based reagents within microfluidic analysis systems |
| US7745180B2 (en) | 2002-04-24 | 2010-06-29 | Hitachi Chemical Co., Ltd. | Device and method for high-throughput quantification of mRNA from whole blood |
-
2001
- 2001-10-25 AT AT01270141T patent/ATE480618T1/de not_active IP Right Cessation
- 2001-10-25 DE DE60143046T patent/DE60143046D1/de not_active Expired - Lifetime
- 2001-10-25 EP EP01270141A patent/EP1330518B1/de not_active Expired - Lifetime
- 2001-10-25 WO PCT/US2001/049498 patent/WO2002066637A2/en not_active Ceased
- 2001-10-25 JP JP2002566343A patent/JP5416326B2/ja not_active Expired - Fee Related
- 2001-10-25 CN CNB018213944A patent/CN1294262C/zh not_active Expired - Fee Related
- 2001-10-25 US US10/399,798 patent/US7374881B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP1330518A2 (de) | 2003-07-30 |
| US7374881B2 (en) | 2008-05-20 |
| EP1330518B1 (de) | 2010-09-08 |
| JP2004519234A (ja) | 2004-07-02 |
| WO2002066637A2 (en) | 2002-08-29 |
| DE60143046D1 (de) | 2010-10-21 |
| JP5416326B2 (ja) | 2014-02-12 |
| CN1606621A (zh) | 2005-04-13 |
| WO2002066637A3 (en) | 2003-05-22 |
| CN1294262C (zh) | 2007-01-10 |
| WO2002066637A8 (en) | 2003-10-30 |
| US20040072193A1 (en) | 2004-04-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |