ATE444355T1 - Mutagenese-screeningverfahren mit hohem durchsatz - Google Patents
Mutagenese-screeningverfahren mit hohem durchsatzInfo
- Publication number
- ATE444355T1 ATE444355T1 AT03744218T AT03744218T ATE444355T1 AT E444355 T1 ATE444355 T1 AT E444355T1 AT 03744218 T AT03744218 T AT 03744218T AT 03744218 T AT03744218 T AT 03744218T AT E444355 T1 ATE444355 T1 AT E444355T1
- Authority
- AT
- Austria
- Prior art keywords
- mutagenesis methods
- provides
- variants
- screening methods
- cutinase
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 4
- 238000002703 mutagenesis Methods 0.000 title abstract 4
- 231100000350 mutagenesis Toxicity 0.000 title abstract 4
- 238000012216 screening Methods 0.000 title abstract 3
- 108010005400 cutinase Proteins 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 238000010276 construction Methods 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 238000011156 evaluation Methods 0.000 abstract 1
- 230000009897 systematic effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/01—Carboxylic ester hydrolases (3.1.1)
- C12Y301/01074—Cutinase (3.1.1.74)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Analysing Materials By The Use Of Radiation (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US36237202P | 2002-03-05 | 2002-03-05 | |
| US10/091,912 US20030199068A1 (en) | 2002-03-05 | 2002-03-05 | High throughput mutagenesis screening method |
| US10/092,227 US20030171543A1 (en) | 2002-03-05 | 2002-03-05 | High throughput mutagenesis screening method |
| US40392102P | 2002-08-15 | 2002-08-15 | |
| PCT/US2003/006908 WO2003076580A2 (en) | 2002-03-05 | 2003-03-05 | High throughput mutagenesis screening method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE444355T1 true ATE444355T1 (de) | 2009-10-15 |
Family
ID=27808762
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT03744218T ATE444355T1 (de) | 2002-03-05 | 2003-03-05 | Mutagenese-screeningverfahren mit hohem durchsatz |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP1543117B1 (de) |
| AT (1) | ATE444355T1 (de) |
| AU (1) | AU2003216540A1 (de) |
| DE (1) | DE60329504D1 (de) |
| ES (1) | ES2333789T3 (de) |
| PT (1) | PT1543117E (de) |
| WO (1) | WO2003076580A2 (de) |
Families Citing this family (73)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BRPI0709978A2 (pt) | 2006-04-14 | 2011-08-02 | Genencor Int | tratamento em etapa única de produtos têxteis |
| RU2520808C2 (ru) * | 2007-06-06 | 2014-06-27 | ДАНИСКО ЮЭс, ИНК., ДЖЕНЕНКОР ДИВИЖН | Способ идентификации улучшенных вариантов белка |
| KR20100028031A (ko) | 2007-06-06 | 2010-03-11 | 다니스코 유에스 인크. | 단백질 특성의 개선 방법 |
| EP2537918A1 (de) | 2011-06-20 | 2012-12-26 | The Procter & Gamble Company | Verbraucherprodukte mit lipasenhaltigen beschichteten Partikeln |
| WO2012175401A2 (en) | 2011-06-20 | 2012-12-27 | Novozymes A/S | Particulate composition |
| WO2013171072A1 (en) * | 2012-05-14 | 2013-11-21 | Novozymes A/S | Cutinase variants and polynucleotides encoding same |
| EP2767582A1 (de) | 2013-02-19 | 2014-08-20 | The Procter and Gamble Company | Verfahren zum Waschen eines Stoffs |
| HUE052331T2 (hu) | 2013-02-19 | 2021-04-28 | Procter & Gamble | Textiltisztítási eljárás |
| EP2767579B1 (de) | 2013-02-19 | 2018-07-18 | The Procter and Gamble Company | Verfahren zum Waschen eines Stoffs |
| WO2015085920A1 (en) | 2013-12-11 | 2015-06-18 | Novozymes A/S | Cutinase variants and polynucleotides encoding same |
| EP2987849A1 (de) | 2014-08-19 | 2016-02-24 | The Procter and Gamble Company | Verfahren zum Waschen eines Stoffs |
| EP2987848A1 (de) | 2014-08-19 | 2016-02-24 | The Procter & Gamble Company | Verfahren zum Waschen eines Stoffs |
| BR112017027405B1 (pt) | 2015-06-26 | 2022-05-10 | Unilever Ip Holdings B.V. | Composição de detergente para lavagem de roupas e método de tratamento doméstico de um tecido |
| WO2019092191A1 (en) | 2017-11-13 | 2019-05-16 | Unilever Plc | Method of demonstrating sebum removal from laundered garments |
| WO2020058091A1 (en) | 2018-09-18 | 2020-03-26 | Unilever Plc | Method of chemical monitoring the fat removal from surfaces |
| US20220033736A1 (en) | 2018-09-18 | 2022-02-03 | Conopco, Inc., D/B/A Unilever | Detergent composition |
| WO2020193101A1 (en) | 2019-03-22 | 2020-10-01 | Unilever Plc | Method for washing a garment worn on the head |
| WO2020259949A1 (en) | 2019-06-28 | 2020-12-30 | Unilever Plc | Detergent composition |
| WO2022197810A1 (en) * | 2021-03-17 | 2022-09-22 | Danisco Us Inc | Variant enzymes and uses thereof |
| WO2023278297A1 (en) | 2021-06-30 | 2023-01-05 | Danisco Us Inc | Variant lipases and uses thereof |
| CN117580940A (zh) * | 2021-06-30 | 2024-02-20 | 汉高股份有限及两合公司 | 包含具有聚酯酶活性的脂肪分解酶的清洁组合物 |
| US20240240115A1 (en) * | 2021-06-30 | 2024-07-18 | Henkel Ag & Co. Kgaa | Composition with improved moisture management performance |
| EP4363544A1 (de) * | 2021-06-30 | 2024-05-08 | Henkel AG & Co. KGaA | Reinigungszusammensetzung mit verbesserter anti-grauungs- und/oder anti-tödungsleistung |
| US20240352499A1 (en) | 2021-08-20 | 2024-10-24 | Danisco Us Inc. | Polynucleotides encoding novel nucleases, compositions thereof and methods thereof for eliminating dna from protein preparations |
| CN116265485B (zh) * | 2021-12-17 | 2026-04-21 | 深圳先进技术研究院 | 结合单点饱和突变扫描和多点定向组合突变的蛋白质工程方法 |
| CN119301230A (zh) | 2022-05-27 | 2025-01-10 | 联合利华知识产权控股有限公司 | 包含表面活性剂、氨基羧酸盐、有机酸和香料的洗衣液体组合物 |
| WO2023227335A1 (en) | 2022-05-27 | 2023-11-30 | Unilever Ip Holdings B.V. | Liquid composition comprising linear alkyl benzene sulphonate, methyl ester ethoxylate and alkoxylated zwitterionic polyamine polymer |
| WO2023227331A1 (en) | 2022-05-27 | 2023-11-30 | Unilever Ip Holdings B.V. | Composition comprising a specific methyl ester ethoxylate surfactant and a lipase |
| CN119213107A (zh) | 2022-05-27 | 2024-12-27 | 联合利华知识产权控股有限公司 | 包含表面活性剂、烷氧基化两性离子聚胺聚合物和芳香剂的洗衣液体组合物 |
| WO2023227332A1 (en) | 2022-05-27 | 2023-11-30 | Unilever Ip Holdings B.V. | Laundry liquid composition comprising a surfactant, an alkoxylated zwitterionic polyamine polymer and a protease |
| WO2024056332A1 (en) | 2022-09-13 | 2024-03-21 | Unilever Ip Holdings B.V. | Washing machine and washing method |
| EP4587542A1 (de) | 2022-09-13 | 2025-07-23 | Unilever IP Holdings B.V. | Waschmaschine und waschverfahren |
| US20260078316A1 (en) | 2022-09-13 | 2026-03-19 | Conopco, Inc., D/B/A Unilever | Washing machine and washing method |
| WO2024056333A1 (en) | 2022-09-13 | 2024-03-21 | Unilever Ip Holdings B.V. | Washing machine and washing method |
| EP4349947A1 (de) | 2022-10-05 | 2024-04-10 | Unilever IP Holdings B.V. | Flüssigwaschmittelzusammensetzung |
| EP4349945A1 (de) | 2022-10-05 | 2024-04-10 | Unilever IP Holdings B.V. | Flüssigwaschmittelzusammensetzung |
| EP4349948A1 (de) | 2022-10-05 | 2024-04-10 | Unilever IP Holdings B.V. | Flüssigwaschmittelzusammensetzung |
| EP4349946A1 (de) | 2022-10-05 | 2024-04-10 | Unilever IP Holdings B.V. | Einheitsdosisprodukt zur behandlung von textilien |
| EP4349943A1 (de) | 2022-10-05 | 2024-04-10 | Unilever IP Holdings B.V. | Flüssigwaschmittelzusammensetzung |
| EP4349942A1 (de) | 2022-10-05 | 2024-04-10 | Unilever IP Holdings B.V. | Flüssigwaschmittelzusammensetzung |
| EP4349944A1 (de) | 2022-10-05 | 2024-04-10 | Unilever IP Holdings B.V. | Flüssigwaschmittelzusammensetzung |
| EP4361239A1 (de) | 2022-10-25 | 2024-05-01 | Unilever IP Holdings B.V. | Flüssigwaschmittelzusammensetzung |
| WO2024088706A1 (en) | 2022-10-25 | 2024-05-02 | Unilever Ip Holdings B.V. | Composition |
| EP4608955A1 (de) | 2022-10-25 | 2025-09-03 | Unilever IP Holdings B.V. | Zusammensetzung |
| EP4627028A1 (de) | 2022-11-29 | 2025-10-08 | Unilever IP Holdings B.V. | Zusammensetzung |
| WO2024194098A1 (en) | 2023-03-21 | 2024-09-26 | Unilever Ip Holdings B.V. | Detergent unit dose |
| WO2024213430A1 (en) | 2023-04-11 | 2024-10-17 | Unilever Ip Holdings B.V. | Composition |
| EP4695363A1 (de) | 2023-04-11 | 2026-02-18 | Unilever IP Holdings B.V. | Zusammensetzung |
| EP4695360A1 (de) | 2023-04-11 | 2026-02-18 | Unilever IP Holdings B.V. | Zusammensetzung |
| EP4695362A1 (de) | 2023-04-11 | 2026-02-18 | Unilever IP Holdings B.V. | Zusammensetzung |
| EP4695364A1 (de) | 2023-04-11 | 2026-02-18 | Unilever IP Holdings B.V. | Zusammensetzung |
| WO2024223218A1 (en) | 2023-04-25 | 2024-10-31 | Unilever Ip Holdings B.V. | Composition |
| EP4662299A1 (de) | 2023-07-11 | 2025-12-17 | Unilever IP Holdings B.V. | Verfahren zur behandlung von gewebe |
| PL4662298T1 (pl) | 2023-07-11 | 2026-01-12 | Unilever Ip Holdings B.V. | Sposób traktowania materiału włókienniczego |
| CN121488025A (zh) | 2023-07-13 | 2026-02-06 | 联合利华知识产权控股有限公司 | 洗衣机和方法 |
| WO2025016669A1 (en) | 2023-07-19 | 2025-01-23 | Unilever Ip Holdings B.V. | Laundry capsule |
| CN121773188A (zh) | 2023-08-02 | 2026-03-31 | 联合利华知识产权控股有限公司 | 组合物 |
| WO2025031752A1 (en) | 2023-08-04 | 2025-02-13 | Unilever Ip Holdings B.V. | Composition |
| CN121729476A (zh) | 2023-08-04 | 2026-03-24 | 联合利华知识产权控股有限公司 | 组合物 |
| EP4509589A1 (de) | 2023-08-16 | 2025-02-19 | Unilever IP Holdings B.V. | Einheitsdosisprodukt |
| WO2025124811A1 (en) | 2023-12-14 | 2025-06-19 | Unilever Ip Holdings B.V. | Composition |
| EP4570890A1 (de) | 2023-12-14 | 2025-06-18 | Unilever IP Holdings B.V. | Zusammensetzung |
| EP4574951A1 (de) | 2023-12-20 | 2025-06-25 | The Procter & Gamble Company | Verfahren zum waschen von stoffen bei niedrigen temperaturen |
| EP4574943A1 (de) | 2023-12-20 | 2025-06-25 | The Procter & Gamble Company | Verfahren zum waschen von stoffen bei niedrigen temperaturen |
| EP4574941A1 (de) | 2023-12-20 | 2025-06-25 | The Procter & Gamble Company | Verfahren zum waschen von stoffen bei niedrigen temperaturen |
| WO2025214659A1 (en) | 2024-04-11 | 2025-10-16 | Unilever Ip Holdings B.V. | Washing method |
| WO2025214720A1 (en) | 2024-04-11 | 2025-10-16 | Unilever Ip Holdings B.V. | Washing machine and washing method |
| EP4663737A1 (de) | 2024-06-13 | 2025-12-17 | Unilever IP Holdings B.V. | Wäscheeinheitsdosisprodukt |
| EP4663738A1 (de) | 2024-06-13 | 2025-12-17 | Unilever IP Holdings B.V. | Wäscheeinheitsdosisprodukt |
| EP4663728A1 (de) | 2024-06-13 | 2025-12-17 | Unilever IP Holdings B.V. | Verfahren zur behandlung von textilien |
| EP4663729A1 (de) | 2024-06-13 | 2025-12-17 | Unilever IP Holdings B.V. | Verfahren zur behandlung von textilien |
| WO2026012788A1 (en) | 2024-07-08 | 2026-01-15 | Unilever Ip Holdings B.V. | Composition |
| WO2026012789A1 (en) | 2024-07-08 | 2026-01-15 | Unilever Ip Holdings B.V. | Composition |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5352594A (en) * | 1984-05-29 | 1994-10-04 | Genecor, Inc. | Selection and method of making enzymes for perhydrolysis system and for altering substrate specificity, specific activity and catalytic efficiency |
| AU621813B2 (en) * | 1986-11-19 | 1992-03-26 | Genencor Inc. | Novel hydrolase and method |
-
2003
- 2003-03-05 AT AT03744218T patent/ATE444355T1/de active
- 2003-03-05 AU AU2003216540A patent/AU2003216540A1/en not_active Abandoned
- 2003-03-05 DE DE60329504T patent/DE60329504D1/de not_active Expired - Lifetime
- 2003-03-05 ES ES03744218T patent/ES2333789T3/es not_active Expired - Lifetime
- 2003-03-05 EP EP03744218A patent/EP1543117B1/de not_active Expired - Lifetime
- 2003-03-05 WO PCT/US2003/006908 patent/WO2003076580A2/en not_active Ceased
- 2003-03-05 PT PT03744218T patent/PT1543117E/pt unknown
Also Published As
| Publication number | Publication date |
|---|---|
| ES2333789T3 (es) | 2010-03-01 |
| EP1543117A4 (de) | 2007-06-20 |
| AU2003216540A8 (en) | 2003-09-22 |
| PT1543117E (pt) | 2010-01-07 |
| WO2003076580A2 (en) | 2003-09-18 |
| EP1543117B1 (de) | 2009-09-30 |
| EP1543117A2 (de) | 2005-06-22 |
| DE60329504D1 (de) | 2009-11-12 |
| AU2003216540A1 (en) | 2003-09-22 |
| WO2003076580A3 (en) | 2005-04-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |
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