ATE449182T1 - Gen-silencing-vektor und diesen verwendendes gen- silencing-verfahren - Google Patents

Gen-silencing-vektor und diesen verwendendes gen- silencing-verfahren

Info

Publication number
ATE449182T1
ATE449182T1 AT03752935T AT03752935T ATE449182T1 AT E449182 T1 ATE449182 T1 AT E449182T1 AT 03752935 T AT03752935 T AT 03752935T AT 03752935 T AT03752935 T AT 03752935T AT E449182 T1 ATE449182 T1 AT E449182T1
Authority
AT
Austria
Prior art keywords
vector
gene
gen
silencing
same
Prior art date
Application number
AT03752935T
Other languages
English (en)
Inventor
Keisuke Kasaoka
Yasuhito Saito
Shigeru Kuwata
Original Assignee
Japan Tobacco Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Japan Tobacco Inc filed Critical Japan Tobacco Inc
Application granted granted Critical
Publication of ATE449182T1 publication Critical patent/ATE449182T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Compositions Of Oxide Ceramics (AREA)
  • Inorganic Insulating Materials (AREA)
  • Saccharide Compounds (AREA)
AT03752935T 2002-05-22 2003-05-21 Gen-silencing-vektor und diesen verwendendes gen- silencing-verfahren ATE449182T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2002147888 2002-05-22
PCT/JP2003/006328 WO2003097841A1 (fr) 2002-05-22 2003-05-21 Vecteur de silençage genique et procede de silençage genique utilisant ce vecteur

Publications (1)

Publication Number Publication Date
ATE449182T1 true ATE449182T1 (de) 2009-12-15

Family

ID=29545204

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03752935T ATE449182T1 (de) 2002-05-22 2003-05-21 Gen-silencing-vektor und diesen verwendendes gen- silencing-verfahren

Country Status (9)

Country Link
EP (1) EP1507009B1 (de)
JP (1) JP4361858B2 (de)
AT (1) ATE449182T1 (de)
AU (1) AU2003235377A1 (de)
DE (1) DE60330140D1 (de)
DK (1) DK1507009T3 (de)
ES (1) ES2335756T3 (de)
PT (1) PT1507009E (de)
WO (1) WO2003097841A1 (de)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110857438B (zh) * 2018-08-20 2022-05-17 中国烟草总公司黑龙江省公司牡丹江烟草科学研究所 一种高效产生siRNA的烟草花叶病毒基因片段、弱毒疫苗、制备方法及其应用
CN110885797B (zh) * 2018-08-20 2022-04-12 山东农业大学 一种抗黄瓜花叶病毒的弱毒疫苗、制备方法及其应用
CN110857439B (zh) * 2018-08-20 2022-05-17 中国烟草总公司黑龙江省公司牡丹江烟草科学研究所 一种高效产生siRNA的马铃薯Y病毒基因片段、弱毒疫苗、制备方法及其应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3452372B2 (ja) * 1992-10-21 2003-09-29 日本たばこ産業株式会社 組換えベクター及びそれを用いてジャガイモにpvy−tに対する免疫性を付与する方法並びにpvy−t免疫性ジャガイモ
GB9703146D0 (en) * 1997-02-14 1997-04-02 Innes John Centre Innov Ltd Methods and means for gene silencing in transgenic plants
EP1456389A1 (de) * 2001-12-12 2004-09-15 Plant Bioscience Limited Verfharen zur genabschaltung (gene silencing) in pflanzen

Also Published As

Publication number Publication date
ES2335756T3 (es) 2010-04-05
EP1507009B1 (de) 2009-11-18
DE60330140D1 (de) 2009-12-31
AU2003235377A1 (en) 2003-12-02
EP1507009A4 (de) 2006-03-22
PT1507009E (pt) 2010-01-25
EP1507009A1 (de) 2005-02-16
JP4361858B2 (ja) 2009-11-11
JPWO2003097841A1 (ja) 2005-09-15
DK1507009T3 (da) 2010-04-06
WO2003097841A1 (fr) 2003-11-27

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