ATE450606T1 - MULTIPLE SUBSTITUTED PROTEASE VARIANTS - Google Patents

MULTIPLE SUBSTITUTED PROTEASE VARIANTS

Info

Publication number
ATE450606T1
ATE450606T1 AT03731960T AT03731960T ATE450606T1 AT E450606 T1 ATE450606 T1 AT E450606T1 AT 03731960 T AT03731960 T AT 03731960T AT 03731960 T AT03731960 T AT 03731960T AT E450606 T1 ATE450606 T1 AT E450606T1
Authority
AT
Austria
Prior art keywords
protease
amino acid
precursor
variants
naturally
Prior art date
Application number
AT03731960T
Other languages
German (de)
Inventor
Richard Bott
David Estell
Kellis, Jr
Ayrookaran Poulose
Original Assignee
Genencor Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Int filed Critical Genencor Int
Application granted granted Critical
Publication of ATE450606T1 publication Critical patent/ATE450606T1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38609—Protease or amylase in solid compositions only
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21062—Subtilisin (3.4.21.62)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Meat, Egg Or Seafood Products (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Novel enzyme variants including protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue correspond to positions 27, 45, 170, 181, 251 and 271 of Bacillus amyloliquefaciens subtilisin. Additional variants comprising at least one additional substitution at a position selected from 1, 14, 49, 61, 87, 100, 102, 118, 128, 204 and 258 of Bacillus amyloliquefaciens subtilisin are also described.
AT03731960T 2002-01-16 2003-01-16 MULTIPLE SUBSTITUTED PROTEASE VARIANTS ATE450606T1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US35022202P 2002-01-16 2002-01-16
PCT/US2003/001448 WO2003062381A2 (en) 2002-01-16 2003-01-16 Multiply-substituted protease variants

Publications (1)

Publication Number Publication Date
ATE450606T1 true ATE450606T1 (en) 2009-12-15

Family

ID=27613372

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03731960T ATE450606T1 (en) 2002-01-16 2003-01-16 MULTIPLE SUBSTITUTED PROTEASE VARIANTS

Country Status (13)

Country Link
US (8) US7306937B2 (en)
EP (1) EP1523553B1 (en)
JP (2) JP4703113B2 (en)
AT (1) ATE450606T1 (en)
AU (1) AU2003210552A1 (en)
BR (1) BRPI0306955B1 (en)
CA (2) CA2472725C (en)
DE (1) DE60330346D1 (en)
DK (1) DK1523553T3 (en)
ES (1) ES2336092T3 (en)
MX (1) MXPA04006809A (en)
PT (1) PT1523553E (en)
WO (1) WO2003062381A2 (en)

Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2367505T3 (en) * 1997-10-23 2011-11-04 Danisco Us Inc. PROTEASE VARIANTS WITH MULTIPLE SUBSTITUTIONS WITH ALTERED NET LOAD FOR USE IN DETERGENTS.
DK200101090A (en) * 2001-07-12 2001-08-16 Novozymes As Subtilase variants
DK1523553T3 (en) * 2002-01-16 2010-04-19 Genencor Int Multi-substituted protease variants
US7888093B2 (en) 2002-11-06 2011-02-15 Novozymes A/S Subtilase variants
US7294499B2 (en) * 2003-01-30 2007-11-13 Novozymes A/S Subtilases
EP1735433B1 (en) * 2004-04-02 2010-12-08 Novozymes A/S Subtilase variants having altered immunogenicity
WO2007061886A2 (en) * 2005-11-18 2007-05-31 Verenium Corporation Compositions and methods for ameliorating toxins and virulence factors
US9150626B2 (en) * 2006-06-02 2015-10-06 President And Fellows Of Harvard College Protein surface remodeling
PT2032698E (en) * 2006-06-23 2012-09-24 Danisco Us Inc Systematic evaluation of sequence and activity relationships using site evaluation libraries for engineering multiple properties
WO2008010925A2 (en) * 2006-07-18 2008-01-24 Danisco Us, Inc., Genencor Division Protease variants active over a broad temperature range
BRPI0812448A2 (en) * 2007-06-06 2014-10-14 Danisco Us Inc Genecor Division PROTEIN PROPERTY PROCESSING METHOD
JP2011523353A (en) * 2008-04-28 2011-08-11 プレジデント アンド フェロウズ オブ ハーバード カレッジ Overcharged protein for cell penetration
WO2009149144A2 (en) * 2008-06-06 2009-12-10 Danisco Us Inc. Compositions and methods comprising variant microbial proteases
US20100192985A1 (en) * 2008-11-11 2010-08-05 Wolfgang Aehle Compositions and methods comprising serine protease variants
EP2362896A2 (en) * 2008-11-11 2011-09-07 Danisco US Inc. Bacillus subtilisin comprising one or more combinable mutations
US9221886B2 (en) 2009-04-28 2015-12-29 President And Fellows Of Harvard College Supercharged proteins for cell penetration
DE102009027045A1 (en) * 2009-06-19 2010-12-30 Henkel Ag & Co. Kgaa New proteases and agents containing these proteases
JP5863666B2 (en) * 2009-12-09 2016-02-17 ダニスコ・ユーエス・インク Compositions and methods comprising protease variants
MX2012006616A (en) 2009-12-09 2012-06-21 Procter & Gamble Fabric and home care products.
PL3575389T3 (en) * 2010-05-06 2025-06-02 The Procter & Gamble Company Consumer products with protease variants
FI123942B (en) 2010-10-29 2013-12-31 Ab Enzymes Oy Variants of fungal-derived serine protease
DE102011007313A1 (en) 2011-04-13 2012-10-18 Henkel Ag & Co. Kgaa expression methods
MX357386B (en) * 2011-05-05 2018-07-06 Procter & Gamble Compositions and methods comprising serine protease variants.
CN104011204A (en) * 2011-12-20 2014-08-27 诺维信公司 Subtilase Variants And Polynucleotides Encoding Same
BR112018010475B1 (en) 2015-11-25 2023-02-14 Unilever Ip Holdings B.V. LIQUID DETERGENT COMPOSITION AND LAUNDRY WASHING METHOD USING A LIQUID WASHING DETERGENT COMPOSITION
EP3702452A1 (en) * 2019-03-01 2020-09-02 Novozymes A/S Detergent compositions comprising two proteases
CN110846299B (en) * 2019-11-22 2021-09-24 江南大学 A leader peptide mutant and its application in the production of keratinase
US20250311734A1 (en) 2022-05-14 2025-10-09 Novozymes A/S Compositions and Methods for Preventing, Treating, Suppressing and/or Eliminatting Phytopathogenic Infestations and Infections
WO2025202374A1 (en) 2024-03-27 2025-10-02 Basf Se Polypeptides having protease activity for use in detergent compositions
EP4624572A1 (en) 2024-03-27 2025-10-01 Basf Se Polypeptides having protease activity for use in detergent compositions
WO2025202382A1 (en) 2024-03-27 2025-10-02 Basf Se Polypeptides having protease activity for use in cleaning compositions
WO2025202370A1 (en) 2024-03-27 2025-10-02 Basf Se Polypeptides having protease activity for use in detergent compositions
WO2025202379A1 (en) 2024-03-27 2025-10-02 Basf Se Polypeptides having protease activity for use in detergent compositions
WO2025202372A1 (en) 2024-03-27 2025-10-02 Basf Se Polypeptides having protease activity for use in detergent compositions
WO2025202369A1 (en) 2024-03-27 2025-10-02 Basf Se Polypeptides having protease activity for use in detergent compositions

Family Cites Families (75)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3557002A (en) * 1967-11-15 1971-01-19 Procter & Gamble Stabilized aqueous enzyme preparation
US3558498A (en) * 1967-11-29 1971-01-26 Procter & Gamble Granular detergent composition containing enzymes and environmental control components
GB1240058A (en) * 1968-04-12 1971-07-21 Procter & Gamble Enzyme-containing detergent compositions
US3623957A (en) * 1970-01-21 1971-11-30 Baxter Laboratories Inc Preparation of microbial alkaline protease by fermentation with bacillus subtilis, variety licheniformis
BE785666A (en) * 1971-07-01 1973-01-02 Procter & Gamble PROCESS FOR THE PRODUCTION OF ENZYMATIC COMPOSITIONS IN GRANULES
BE792982A (en) * 1971-12-20 1973-06-19 Procter & Gamble Europ Proteolytic enzymes detergent - contg cationic and anionic surfactants
US4011169A (en) * 1973-06-29 1977-03-08 The Procter & Gamble Company Stabilization and enhancement of enzymatic activity
DE2709476A1 (en) * 1976-03-08 1977-09-15 Procter & Gamble Europ LIQUID, ENZYME-BASED DETERGENT AND DETERGENT
US4090973A (en) * 1976-06-24 1978-05-23 The Procter & Gamble Company Method for making stable detergent compositions
DE2633601A1 (en) * 1976-07-27 1978-02-02 Henkel Kgaa LIQUID, ENZYMATIC CONCENTRATE CAN BE USED AS A WASHING AGENT AND CLEANING AGENT
GB1603640A (en) * 1977-07-20 1981-11-25 Gist Brocades Nv Enzyme particles
US4261868A (en) * 1979-08-08 1981-04-14 Lever Brothers Company Stabilized enzymatic liquid detergent composition containing a polyalkanolamine and a boron compound
US4318818A (en) * 1979-11-09 1982-03-09 The Procter & Gamble Company Stabilized aqueous enzyme composition
JPS5950280B2 (en) * 1980-10-24 1984-12-07 花王株式会社 Enzyme bleach composition
US4404128A (en) * 1981-05-29 1983-09-13 The Procter & Gamble Company Enzyme detergent composition
EP0080748B1 (en) * 1981-11-13 1985-07-10 Unilever N.V. Enzymatic liquid cleaning composition
JPS58144105A (en) * 1982-02-12 1983-08-27 Kurabo Ind Ltd Production of descaled animal fiber
US5310675A (en) * 1983-06-24 1994-05-10 Genencor, Inc. Procaryotic carbonyl hydrolases
US4760025A (en) * 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US5763257A (en) * 1984-05-29 1998-06-09 Genencor International, Inc. Modified subtilisins having amino acid alterations
US5264366A (en) * 1984-05-29 1993-11-23 Genencor, Inc. Protease deficient bacillus
US5182204A (en) * 1984-05-29 1993-01-26 Genencor International, Inc. Non-human carbonyl hydrolase mutants, vectors encoding same and hosts transformed with said vectors
US5155033A (en) * 1989-01-06 1992-10-13 Genencor, Inc. Subtilisins modified at position 225 resulting in a shift in catalytic activity
US5185258A (en) * 1984-05-29 1993-02-09 Genencor International, Inc. Subtilisin mutants
US5204015A (en) * 1984-05-29 1993-04-20 Genencor International, Inc. Subtilisin mutants
US5972682A (en) * 1984-05-29 1999-10-26 Genencor International, Inc. Enzymatically active modified subtilisins
DE3750916T2 (en) * 1986-04-30 1995-06-01 Genencor Int Mutant of a non-human carbonyl hydrolase, DNA sequences and vectors coding therefor and hosts transformed by these vectors.
US5314692A (en) * 1987-08-24 1994-05-24 Cultor Ltd. Enzyme premix for feed and method
DK6488D0 (en) * 1988-01-07 1988-01-07 Novo Industri As ENZYMES
CN1056187C (en) * 1988-02-11 2000-09-06 金克克国际有限公司 Proteolytic enzymes and their use in detergents
US5324653A (en) * 1988-02-11 1994-06-28 Gist-Brocades N.V. Recombinant genetic means for the production of serine protease muteins
US6287841B1 (en) * 1988-02-11 2001-09-11 Genencor International, Inc. High alkaline serine protease
DE3841152A1 (en) * 1988-12-07 1990-06-13 Hoechst Ag USE OF BACTERIA-LYING ENZYME PRODUCT AS AN ADDITIVE TO IMPROVE THE FEED RECYCLING IN ANIMAL PRODUCTION
US5665587A (en) * 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
DK316989D0 (en) * 1989-06-26 1989-06-26 Novo Nordisk As ENZYMES
ES2227508T3 (en) * 1989-06-26 2005-04-01 Unilever N.V. ENZYMATIC DETERGENT COMPOSITIONS.
US5352603A (en) * 1989-08-31 1994-10-04 Kali-Chemie Ag Highly alkaline proteases
EP0995801A1 (en) * 1991-07-27 2000-04-26 Genencor International GmbH Process for increasing the stability of enzymes and stabilized enzymes
AU682314B2 (en) * 1992-07-17 1997-10-02 Genencor International, Inc. High alkaline serine proteases
US6436690B1 (en) * 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
DE69434962T2 (en) * 1993-10-14 2008-01-17 The Procter & Gamble Company, Cincinnati PROTEASE-CONTAINING DETERGENTS
MA23346A1 (en) * 1993-10-14 1995-04-01 Genencor Int VARIANTS OF THE SUB-USE
US6599730B1 (en) * 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
GB9416841D0 (en) * 1994-08-19 1994-10-12 Finnfeeds Int Ltd An enzyme feed additive and animal feed including it
US6682924B1 (en) * 1995-05-05 2004-01-27 Novozymes A/S Protease variants and compositions
US5837517A (en) * 1995-05-05 1998-11-17 Novo Nordisk A/S Protease variants and compositions
CN1530443A (en) * 1996-11-04 2004-09-22 ŵ����÷�����޹�˾ subtilase variants and compositions
WO1998055634A1 (en) * 1997-06-04 1998-12-10 The Procter & Gamble Company Protease enzymes for tough cleaning and/or spot and film reduction and compositions incorporating same
BR9811412A (en) * 1997-08-29 2000-08-22 Novo Nordisk As Subtilase enzyme variant having improved detergent washing performance, isolated DNA sequence, expression vector, microbial host cell, processes for producing a variant and for identifying a protease variant, composition, and, use of a subtilase variant
WO1999011768A1 (en) * 1997-08-29 1999-03-11 Novo Nordisk A/S Protease variants and compositions
EP0913458B1 (en) * 1997-10-22 2004-06-16 The Procter & Gamble Company Liquid hard-surface cleaning compositions
AR016969A1 (en) * 1997-10-23 2001-08-01 Procter & Gamble PROTEASE VARIANTE, ADN, EXPRESSION VECTOR, GUEST MICROORGANISM, CLEANING COMPOSITION, ANIMAL FOOD AND COMPOSITION TO TREAT A TEXTILE
US6773907B2 (en) * 1997-11-21 2004-08-10 Peter Kamp Hansen Subtilase enzymes
ATE298791T1 (en) * 1997-11-21 2005-07-15 Novozymes As PROTEASE VARIANTS AND COMPOSITIONS
US6780629B2 (en) * 1997-11-21 2004-08-24 Novozymes A/S Subtilase enzymes
IT1304768B1 (en) * 1998-10-05 2001-03-29 Esaote Spa TABLE FOR PATIENT HOLDER OR SIMILAR, AND MACHINE, IN PARTICULAR MACHINE FOR DETECTION OF IMAGES IN NUCLEAR MAGNETIC RESONANCE IN
US6831053B1 (en) * 1998-10-23 2004-12-14 The Procter & Gamble Company Bleaching compositions comprising multiply-substituted protease variants
US6376450B1 (en) * 1998-10-23 2002-04-23 Chanchal Kumar Ghosh Cleaning compositions containing multiply-substituted protease variants
AR020951A1 (en) * 1998-10-23 2002-06-05 Procter & Gamble METHODS TO MASK PROTEASE VARIANTS FOR USE IN DETERGENT COMPOSITIONS
CA2376045A1 (en) * 1999-07-22 2001-02-01 The Procter & Gamble Company Subtilisin protease variants having amino acid substitutions in defined epitope regions
US6727085B2 (en) * 1999-12-15 2004-04-27 Fanoe Tina Sejersgaard Subtilase variants having an improved wash performance on egg stains
US6777218B1 (en) * 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
US6902922B2 (en) * 2000-04-03 2005-06-07 Novozymes A/S Subtilisin variants
CA2406621A1 (en) * 2000-04-28 2001-11-08 Novozymes A/S Protein variants having modified immunogenicity
US7109016B2 (en) * 2000-08-21 2006-09-19 Novozymes A/S Subtilase enzymes
US6541235B1 (en) * 2000-09-29 2003-04-01 University Of Maryland Biotechnology Institute Calcium free subtilisin mutants
US6893855B2 (en) * 2000-10-13 2005-05-17 Novozymes A/S Subtilase variants
PL210859B1 (en) * 2001-03-23 2012-03-30 Genencor Int Proteins producing an altered immunogenic response and methods of making and using the same
US20030157645A1 (en) * 2001-12-21 2003-08-21 Direvo Bio Tech Ag. Subtilisin variants with improved characteristics
DK1523553T3 (en) * 2002-01-16 2010-04-19 Genencor Int Multi-substituted protease variants
US7985569B2 (en) * 2003-11-19 2011-07-26 Danisco Us Inc. Cellulomonas 69B4 serine protease variants
WO2008010925A2 (en) * 2006-07-18 2008-01-24 Danisco Us, Inc., Genencor Division Protease variants active over a broad temperature range
WO2009149144A2 (en) * 2008-06-06 2009-12-10 Danisco Us Inc. Compositions and methods comprising variant microbial proteases
EP2362896A2 (en) * 2008-11-11 2011-09-07 Danisco US Inc. Bacillus subtilisin comprising one or more combinable mutations
US20100192985A1 (en) * 2008-11-11 2010-08-05 Wolfgang Aehle Compositions and methods comprising serine protease variants

Also Published As

Publication number Publication date
MXPA04006809A (en) 2004-10-11
US20110091959A1 (en) 2011-04-21
US20050221461A1 (en) 2005-10-06
BRPI0306955B1 (en) 2016-03-22
JP2011041571A (en) 2011-03-03
WO2003062381A2 (en) 2003-07-31
US20080124783A1 (en) 2008-05-29
JP2005519592A (en) 2005-07-07
EP1523553A2 (en) 2005-04-20
EP1523553A4 (en) 2006-06-21
PT1523553E (en) 2010-03-03
US7306937B2 (en) 2007-12-11
EP1523553B1 (en) 2009-12-02
ES2336092T3 (en) 2010-04-08
JP4703113B2 (en) 2011-06-15
DK1523553T3 (en) 2010-04-19
US20130171717A1 (en) 2013-07-04
US20080176313A1 (en) 2008-07-24
US20110086412A1 (en) 2011-04-14
CA2894330A1 (en) 2003-07-31
CA2472725C (en) 2015-08-11
WO2003062381A3 (en) 2005-02-24
AU2003210552A1 (en) 2003-09-02
DE60330346D1 (en) 2010-01-14
US20110091958A1 (en) 2011-04-21
US20090011489A1 (en) 2009-01-08
CA2472725A1 (en) 2003-07-31
BR0306955A (en) 2006-12-19

Similar Documents

Publication Publication Date Title
BR0306955A (en) protease variants with multiple substitutions
WO1999020769A3 (en) Multiply-substituted protease variants
MA23346A1 (en) VARIANTS OF THE SUB-USE
BR9407833A (en) Whitening compositions comprising protease enzymes
AU2002302564A1 (en) Alkaline protease variants and detergents and cleaning agents containing said alkaline protease variants
MX2010004371A (en) Streptomyces protease.
HUP0500058A2 (en) Novel alkaline protease variants and detergents and cleansers containing these novel alkaline protease variants
EP1350843A3 (en) Alkaline cellulase variants
MXPA04006811A (en) Multiply-substituted protease variants.
DK1546318T3 (en) Microbial trypsin variants having chymotrypsin activity and nucleic acids encoding the same
WO2000001826A3 (en) Stabilized variants of streptomyces subtilisin inhibitor
CO4290456A1 (en) CLEANING COMPOSITIONS AND CLEANING METHODS INCLUDING A VARIANT OF CARBONYL HYDROLASE THAT HAS A SEQUENCE OF AMINO ACIDS NOT FOUND IN NATURE, DERIVED FROM A PRECURSING CARBONYL HYDROLASE

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1523553

Country of ref document: EP