ATE452973T1 - Genetische veränderung von somatischen zellen und deren verwendungen - Google Patents

Genetische veränderung von somatischen zellen und deren verwendungen

Info

Publication number
ATE452973T1
ATE452973T1 AT00907792T AT00907792T ATE452973T1 AT E452973 T1 ATE452973 T1 AT E452973T1 AT 00907792 T AT00907792 T AT 00907792T AT 00907792 T AT00907792 T AT 00907792T AT E452973 T1 ATE452973 T1 AT E452973T1
Authority
AT
Austria
Prior art keywords
somatic cells
genetic modification
cells
genetically modified
loci
Prior art date
Application number
AT00907792T
Other languages
English (en)
Inventor
Alan Colman
Angelika Elisabeth Schnieke
Alexander Jarvis Kind
David Lee Ayares
Yifan Dai
Original Assignee
Revivicor Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9905033.8A external-priority patent/GB9905033D0/en
Priority claimed from GBGB9917023.5A external-priority patent/GB9917023D0/en
Priority claimed from US09/475,674 external-priority patent/US20020012660A1/en
Application filed by Revivicor Inc filed Critical Revivicor Inc
Application granted granted Critical
Publication of ATE452973T1 publication Critical patent/ATE452973T1/de

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04L—TRANSMISSION OF DIGITAL INFORMATION, e.g. TELEGRAPHIC COMMUNICATION
    • H04L41/00—Arrangements for maintenance, administration or management of data switching networks, e.g. of packet switching networks
    • H04L41/02—Standardisation; Integration
    • H04L41/0213—Standardised network management protocols, e.g. simple network management protocol [SNMP]
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04L—TRANSMISSION OF DIGITAL INFORMATION, e.g. TELEGRAPHIC COMMUNICATION
    • H04L41/00—Arrangements for maintenance, administration or management of data switching networks, e.g. of packet switching networks
    • H04L41/22—Arrangements for maintenance, administration or management of data switching networks, e.g. of packet switching networks comprising specially adapted graphical user interfaces [GUI]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/10—Conditioning of cells for in vitro fecondation or nuclear transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/60—Vectors containing traps for, e.g. exons, promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
    • C12N2830/85—Vector systems having a special element relevant for transcription from vertebrates mammalian
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Animal Husbandry (AREA)
  • Microbiology (AREA)
  • Veterinary Medicine (AREA)
  • Physics & Mathematics (AREA)
  • Signal Processing (AREA)
  • Biophysics (AREA)
  • Computer Networks & Wireless Communication (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Cell Biology (AREA)
  • Human Computer Interaction (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT00907792T 1999-03-04 2000-03-03 Genetische veränderung von somatischen zellen und deren verwendungen ATE452973T1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GBGB9905033.8A GB9905033D0 (en) 1999-03-04 1999-03-04 Methods
GBGB9917023.5A GB9917023D0 (en) 1999-07-20 1999-07-20 Methods
US09/475,674 US20020012660A1 (en) 1999-03-04 1999-12-30 Method of preparing a somatic cells for nuclear transfer
PCT/GB2000/000778 WO2000051424A2 (en) 1999-03-04 2000-03-03 Genetic modification of somatic cells and uses thereof

Publications (1)

Publication Number Publication Date
ATE452973T1 true ATE452973T1 (de) 2010-01-15

Family

ID=27269662

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00907792T ATE452973T1 (de) 1999-03-04 2000-03-03 Genetische veränderung von somatischen zellen und deren verwendungen

Country Status (11)

Country Link
EP (2) EP2199392A3 (de)
JP (2) JP2002537785A (de)
AT (1) ATE452973T1 (de)
AU (1) AU780754B2 (de)
CA (1) CA2361943C (de)
DE (1) DE60043578D1 (de)
DK (1) DK1159415T3 (de)
ES (1) ES2338631T3 (de)
NZ (1) NZ514620A (de)
PT (1) PT1159415E (de)
WO (1) WO2000051424A2 (de)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7414170B2 (en) 1999-11-19 2008-08-19 Kirin Beer Kabushiki Kaisha Transgenic bovines capable of human antibody production
US7074983B2 (en) 1999-11-19 2006-07-11 Kirin Beer Kabushiki Kaisha Transgenic bovine comprising human immunoglobulin loci and producing human immunoglobulin
US7491534B2 (en) 2000-12-22 2009-02-17 Kirin Holdings Kabushiki Kaisha Methods for altering cell fate to generate T-cells specific for an antigen of interest
MXPA03005624A (es) 2000-12-22 2004-12-03 Vaurox Llc Metodos para clonar mamiferos usando cromatina reprogramada del donador o celulas donadoras.
JP3842597B2 (ja) * 2001-08-14 2006-11-08 家畜受精卵移植技術研究組合 Ｇ１期細胞の収集方法及び該細胞を用いた核移植方法
WO2003075856A2 (en) 2002-03-07 2003-09-18 University Of Delaware Methods, compositions, and kits for enhancing oligonucleotide-mediated nucleic acid sequence alteration using compositions comprising a histone deacetylase inhibitor, lambda phage beta protein, or hydroxyurea
DK1534819T3 (da) 2002-08-21 2010-04-19 Revivicor Inc Griselignende dyr, som mangler enhver ekspression af funktionel alfa-1,3-galactosyltransferase
US7429690B2 (en) 2002-11-08 2008-09-30 Kirin Holdings Kabushiki Kaisha Transgenic bovines having reduced prion protein production
EP1609857B1 (de) * 2003-03-06 2010-10-20 Toudai Tlo, Ltd. Verfahren zur demethylierung einer methylierten zielregion genomischer dna
EP1639083A4 (de) 2003-06-06 2008-05-28 Univ Pittsburgh Gen der cmp-n-acetylneuraminsäure-hydroxylase aus schwein
WO2005047469A2 (en) 2003-11-05 2005-05-26 University Of Pittsburgh PORCINE ISOGLOBOSIDE 3 SYNTHASE PROTEIN, cDNA, GENOMIC ORGANIZATION, AND REGULATORY REGION
AU2005223617A1 (en) 2004-03-17 2005-09-29 Revivicor, Inc. Tissue products from animals lacking functional alpha 1,3 galactosyl transferase
AU2005237494B2 (en) 2004-04-22 2010-06-10 Kyowa Hakko Kirin Co., Ltd Transgenic animals and uses thereof
US7732180B2 (en) 2004-05-07 2010-06-08 University Of Pittsburgh Of The Commonwealth System Of Higher Education Porcine Forssman synthetase protein, cDNA, genomic organization, and regulatory region
US20080026457A1 (en) 2004-10-22 2008-01-31 Kevin Wells Ungulates with genetically modified immune systems
CA2958259C (en) 2004-10-22 2020-06-30 Revivicor, Inc. Ungulates with genetically modified immune systems
EP3138403A1 (de) 2005-08-09 2017-03-08 Revivicor, Inc. Ctla4-ig exprimierende transgene huftiere und verwendungen davon
WO2007117657A2 (en) 2006-04-07 2007-10-18 The Research Foundation Of State University Of New York Transcobalamin receptor polypeptides, nucleic acids, and modulators thereof, and related methods of use in modulating cell growth and treating cancer and cobalamin deficiency
WO2007147167A2 (en) 2006-06-16 2007-12-21 Porous Power Technologies, Llc Optimized microporous structure of electrochemical cells
JP5327733B2 (ja) * 2007-09-18 2013-10-30 国立大学法人名古屋大学 魚類胚の作製方法及びその利用
DK2348827T3 (en) 2008-10-27 2015-07-20 Revivicor Inc IMMUNICIPLY COMPROMATED PETS
JP2012527738A (ja) 2009-05-20 2012-11-08 ポーラス パワー テクノロジーズ，エルエルシー 微多孔膜の処理と接着剤
KR101236724B1 (ko) 2010-11-23 2013-02-26 대한민국 단백질 과발현 카세트를 포함하는 유전자 타겟팅 넉인 벡터, 이의 제조 방법 및 이 벡터가 도입된 이종간 이식용 형질전환 복제동물
ES2680636T3 (es) 2011-02-14 2018-09-10 Revivicor Inc. Cerdos genéticamente modificados para xenotrasplante de xenoinjertos vascularizados y derivados de los mismos
US20220211018A1 (en) 2020-11-20 2022-07-07 Revivicor, Inc. Multi-transgenic pigs with growth hormone receptor knockout for xenotransplantation
CA3232376A1 (en) 2021-09-20 2023-03-23 Maria KOKKINAKI Multitransgenic pigs comprising ten genetic modifications for xenotransplantation
JP2023131904A (ja) * 2022-03-10 2023-09-22 国立大学法人愛媛大学 細胞の移植方法
WO2025012119A1 (en) 2023-07-07 2025-01-16 Technische Universitaet Muenchen Transgenic pig comprising a transgene for xenotransplantation

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993010234A1 (en) * 1991-11-15 1993-05-27 The Regents Of The University Of California Therapy of central nervous system by genetically modified cells
KR960703170A (ko) * 1993-06-23 1996-06-19 알버트 디. 프리센. 안티센스 올리고뉴클레오티드 및 인간면역결핍바이러스감염에서 그것의 치료적이용(antisense oligonucleotides and therapeutic use thereof in human immunodeficiency virus infection)
GB9517779D0 (en) * 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
GB9517780D0 (en) * 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
US5994619A (en) 1996-04-01 1999-11-30 University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts, As Represented By Its Amherst Campus Production of chimeric bovine or porcine animals using cultured inner cell mass cells
US5905042A (en) 1996-04-01 1999-05-18 University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts, As Represented By Its Amherst Campus Cultured inner cell mass cell lines derived from bovine or porcine embryos
WO1997038710A1 (en) * 1996-04-12 1997-10-23 Fibrogen, Inc. Synthesis of human procollagens and collagens in recombinant dna systems
CA2262817A1 (en) 1996-08-19 1998-02-26 James Robl Embryonic or stem-like cell lines produced by cross species nuclear transplantation
US6225290B1 (en) * 1996-09-19 2001-05-01 The Regents Of The University Of California Systemic gene therapy by intestinal cell transformation
JPH10146193A (ja) * 1996-09-23 1998-06-02 Chihiro Koike 相同組換え用ｄｎａコンストラクト、このコンストラクトを用いた形質転換体の選別方法及び相同組換え法
US6235969B1 (en) 1997-01-10 2001-05-22 University Of Massachusetts Cloning pigs using donor nuclei from non-quiescent differentiated cells
US5945577A (en) * 1997-01-10 1999-08-31 University Of Massachusetts As Represented By Its Amherst Campus Cloning using donor nuclei from proliferating somatic cells
KR100570935B1 (ko) * 1997-01-17 2006-04-13 맥시겐, 인크. 반복적 서열 재조합에 의한 전세포 및 유기체의 개량 방법
GB9703550D0 (en) * 1997-02-20 1997-04-09 Ppl Therapeutics Scotland Ltd Cell mediated transgenesis
AU745334B2 (en) * 1997-03-06 2002-03-21 Advanced Cell Technology, Inc. Method of cloning animals
WO1998056903A1 (en) * 1997-06-13 1998-12-17 President And Fellows Of Harvard College Methods and uses for transposon-based gene targeting
IL133786A0 (en) 1997-07-03 2001-04-30 Univ Massachusetts Cloning using donor nuclei from non-serum starved, differentiated cells
GB9715064D0 (en) 1997-07-17 1997-09-24 Ppl Therapeutics Scotland Ltd Protein expression

Also Published As

Publication number Publication date
WO2000051424A3 (en) 2001-03-22
EP1159415A2 (de) 2001-12-05
DK1159415T3 (da) 2010-05-03
CA2361943C (en) 2011-05-17
JP2002537785A (ja) 2002-11-12
AU780754B2 (en) 2005-04-14
AU2926700A (en) 2000-09-21
DE60043578D1 (de) 2010-02-04
NZ514620A (en) 2004-04-30
ES2338631T3 (es) 2010-05-11
CA2361943A1 (en) 2000-09-08
PT1159415E (pt) 2010-03-31
WO2000051424A2 (en) 2000-09-08
EP2199392A3 (de) 2010-12-15
JP2011188873A (ja) 2011-09-29
EP2199392A2 (de) 2010-06-23
EP1159415B1 (de) 2009-12-23

Similar Documents

Publication Publication Date Title
ATE452973T1 (de) Genetische veränderung von somatischen zellen und deren verwendungen
ES2626025T3 (es) Meganucleasas diseñadas racionalmente con especificidad de secuencia y afinidad de unión a ADN alteradas
Zhou et al. Generation of CRISPR/Cas9-mediated gene-targeted pigs via somatic cell nuclear transfer
Zhou et al. Efficient generation of gene‐modified pigs harboring precise orthologous human mutation via CRISPR/Cas9‐induced homology‐directed repair in zygotes
Inoue et al. Effects of donor cell type and genotype on the efficiency of mouse somatic cell cloning
Fujihara et al. CRISPR/Cas9-based genome editing in mice by single plasmid injection
DE60236182D1 (de) Zufällige integration von polynukleotide nach in vivo linearisierung
GB2432846B (en) Medium and culture of embryonic stem cells
BR0209989A (pt) Plataformas baseadas em cromossomas
ATE426661T1 (de) Somatische pluripotente zellen
US20160208214A1 (en) Methods and compositions for generation of developmentally-incompetent eggs in recipients of nuclear genetic transfer
ATE381614T1 (de) Bildung von xenogenen antikörpern
EA200501774A1 (ru) Культуры e1-иммортализованных клеток и способы их культивирования с целью повышения выхода их продукции
CN104611368B (zh) 重组后不产生移码突变的载体、在爪蛙基因组中进行基因定点敲入的方法及应用
ATE416626T1 (de) Kombinierte verwendung von mittelkettige fettsäuren enthaltenden triglyceriden und exogenen lipolytischen enzymen als tierfutterzusatzstoffe
Kanatsu-Shinohara et al. Production of transgenic rats via lentiviral transduction and xenogeneic transplantation of spermatogonial stem cells
RU2006146795A (ru) Композиция для стимулирования роста и регенерации клеток, а также способы ее получения
DE69935907D1 (de) Hocheffizienzverfahren und zusammensetzungen zum integrieren von exogener dna in genomische sperma-dna
Huang et al. Opto-CRISPR: new prospects for gene editing and regulation
ATE243741T1 (de) Zufuhr von genprodukten mittels mesangium-zellen
Kouprina et al. Recent advances in chromosome engineering
ES2074560T3 (es) Procedimiento para la produccion de un animal transgenico no humano mediante la introduccion de adn exogeno en celulas animales somaticas y germinales.
Gim et al. Transgenic F2 bovine embryos show stable germline transmission and maintenance of transgene expression through two generations
GB2369828A (en) Nuclear transfer with selected donor cells
ATE268182T1 (de) Transplantations/implantatsvorrichtung und ihre herstellungsmethode

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1159415

Country of ref document: EP