ATE453722T1 - Mittel zur detektion von enzymaktivitäten in biologischen proben und verfahren zu dessen gebrauch - Google Patents

Mittel zur detektion von enzymaktivitäten in biologischen proben und verfahren zu dessen gebrauch

Info

Publication number
ATE453722T1
ATE453722T1 AT98908564T AT98908564T ATE453722T1 AT E453722 T1 ATE453722 T1 AT E453722T1 AT 98908564 T AT98908564 T AT 98908564T AT 98908564 T AT98908564 T AT 98908564T AT E453722 T1 ATE453722 T1 AT E453722T1
Authority
AT
Austria
Prior art keywords
fluorophores
protease
biological samples
detecting
enzym
Prior art date
Application number
AT98908564T
Other languages
English (en)
Inventor
Akira Komoriya
Beverly Packard
Original Assignee
Oncoimmunin Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oncoimmunin Inc filed Critical Oncoimmunin Inc
Application granted granted Critical
Publication of ATE453722T1 publication Critical patent/ATE453722T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
    • G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
    • G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
    • G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
    • G01N2333/96466—Cysteine endopeptidases (3.4.22)
    • G01N2333/96469—Interleukin 1-beta convertase-like enzymes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
AT98908564T 1997-02-20 1998-02-20 Mittel zur detektion von enzymaktivitäten in biologischen proben und verfahren zu dessen gebrauch ATE453722T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/802,981 US6037137A (en) 1997-02-20 1997-02-20 Fluorogenic peptides for the detection of protease activity
PCT/US1998/003000 WO1998037226A1 (en) 1997-02-20 1998-02-20 Compositions for the detection of enzyme activity in biological samples and methods of use thereof

Publications (1)

Publication Number Publication Date
ATE453722T1 true ATE453722T1 (de) 2010-01-15

Family

ID=25185248

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98908564T ATE453722T1 (de) 1997-02-20 1998-02-20 Mittel zur detektion von enzymaktivitäten in biologischen proben und verfahren zu dessen gebrauch

Country Status (8)

Country Link
US (3) US6037137A (de)
EP (1) EP0988394B1 (de)
JP (2) JP4298796B2 (de)
AT (1) ATE453722T1 (de)
AU (1) AU745148B2 (de)
CA (1) CA2280811C (de)
DE (1) DE69841419D1 (de)
WO (1) WO1998037226A1 (de)

Families Citing this family (144)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6576419B1 (en) * 1993-07-23 2003-06-10 University Of Utah Research Foundation Assay procedure using fluorogenic tracers
US20020110834A1 (en) * 1994-11-04 2002-08-15 Benkovic Stephen J. Fluorescent assay for proteolysis
US7312302B2 (en) 1997-02-20 2007-12-25 Oncolmmunin, Inc. Compositions for the detection of enzyme activity in biological samples and methods of use thereof
US6037137A (en) 1997-02-20 2000-03-14 Oncoimmunin, Inc. Fluorogenic peptides for the detection of protease activity
WO2001018238A1 (en) * 1999-09-10 2001-03-15 Oncoimmunin, Inc. Compositions for the detection of enzyme activity in biological samples and methods of use thereof
US6893868B2 (en) * 1997-02-20 2005-05-17 Onco Immunin, Inc. Homo-doubly labeled compositions for the detection of enzyme activity in biological samples
US6756207B1 (en) 1997-02-27 2004-06-29 Cellomics, Inc. System for cell-based screening
US6342611B1 (en) * 1997-10-10 2002-01-29 Cytovia, Inc. Fluorogenic or fluorescent reporter molecules and their applications for whole-cell fluorescence screening assays for capsases and other enzymes and the use thereof
US20070166741A1 (en) * 1998-12-14 2007-07-19 Somalogic, Incorporated Multiplexed analyses of test samples
US20060057573A1 (en) * 2002-02-15 2006-03-16 Somalogic, Inc Methods and reagents for detecting target binding by nucleic acid ligands
US6242246B1 (en) * 1997-12-15 2001-06-05 Somalogic, Inc. Nucleic acid ligand diagnostic Biochip
DE19811618C1 (de) * 1998-03-17 2000-08-31 Andreas Jenne Ribozym codierende DNA und ein Oligonucleotidsubstrat enthaltende Zusammensetzung und Verfahren zur Messung von Transkriptionsraten
US6251583B1 (en) * 1998-04-27 2001-06-26 Schering Corporation Peptide substrates for HCV NS3 protease assays
US6908770B1 (en) * 1998-07-16 2005-06-21 Board Of Regents, The University Of Texas System Fluid based analysis of multiple analytes by a sensor array
US7456007B1 (en) 1998-12-31 2008-11-25 Elan Pharmaceuticals, Inc. β-secretase enzyme compositions and methods
US7115410B1 (en) 1999-02-10 2006-10-03 Elan Pharmaceuticals, Inc. β-secretase enzyme compositions and methods
GB2364059B (en) * 1999-02-10 2004-01-14 Elan Pharm Inc Beta-Secretase enzyme compositions and methods
AU1325201A (en) 1999-07-16 2001-02-05 Board Of Regents, The University Of Texas System Detection system based on an analyte reactive particle
US7022517B1 (en) 1999-07-16 2006-04-04 Board Of Regents, The University Of Texas System Method and apparatus for the delivery of samples to a chemical sensor array
US7514408B1 (en) 1999-12-02 2009-04-07 Elan Pharmaceuticals, Inc. β-secretase enzyme compositions and methods
ATE403145T1 (de) * 2000-01-31 2008-08-15 Univ Texas Tragbare vorrichtung mit einer sensor-array- anordnung
US20020076741A1 (en) * 2000-02-11 2002-06-20 Tencza Sarah Burroughs Peptide biosensors for anthrax protease
KR20030011777A (ko) * 2000-02-28 2003-02-11 다이이치 가가쿠 야쿠힝 가부시키가이샤 장수명 여기형 형광을 이용하는 측정방법
FR2805820B1 (fr) 2000-03-01 2009-09-11 Pasteur Institut Biocapteurs, leur procede d'obtention et leurs applications
AU2001265092A1 (en) * 2000-06-02 2001-12-17 Regents Of The University Of California Profiling of protease specificity using combinatorial fluorogenic substrate libraries
US6600057B2 (en) 2000-12-29 2003-07-29 Kimberly-Clark Worldwide, Inc. Matrix metalloproteinase inhibitors
US7041787B2 (en) * 2000-12-29 2006-05-09 Kimberly-Clark Worldwide, Inc. Design and use of advanced zinc chelating peptides to regulate matrix metalloproteinases
US20020197622A1 (en) * 2001-01-31 2002-12-26 Mcdevitt John T. Method and apparatus for the confinement of materials in a micromachined chemical sensor array
WO2002063270A2 (en) * 2001-02-05 2002-08-15 Board Of Regents, The University Of Texas System The use of mesoscale self-assembly and recognition to effect delivery of sensing reagent for arrayed sensors
WO2003089572A2 (en) * 2002-04-15 2003-10-30 Chiron Corporation Essential and important genes of pseudomonas aeroginosa and the use thereof to design or identify antibacterial agents
EP1402341A4 (de) * 2001-06-15 2004-09-15 Chiron Corp Essentielle und wichtige gene von pseudomonas aeruginosa und verwendung dafür zum entwerfen oder identifizieren von antibakteriellen mitteln
US7291698B2 (en) * 2001-09-04 2007-11-06 Stephen Eliot Zweig Synthetic substrate for high specificity enzymatic assays
US20030119073A1 (en) * 2001-12-21 2003-06-26 Stephen Quirk Sensors and methods of detection for proteinase enzymes
GB0200479D0 (en) * 2002-01-09 2002-02-27 Medical Res Council Fluorogenic protease substrates
US7927871B2 (en) * 2002-01-29 2011-04-19 Oncoimmunin, Inc. Visualization and quantitation of cellular cytotoxicity using cell-permeable fluorogenic protease substrates and caspase activity indicator markers
US20030211548A1 (en) * 2002-01-29 2003-11-13 Oncolmmunin, Inc. Visualization and quantitiation of cellular cytotoxicity using cell-permeable fluorogenic protease substrates and caspase activity indicator markers
US20030147810A1 (en) * 2002-02-01 2003-08-07 University Of Michigan Compositions and methods for reporting of protease activity within the secretory pathway
US8257967B2 (en) 2002-04-26 2012-09-04 Board Of Regents, The University Of Texas System Method and system for the detection of cardiac risk factors
US6942987B2 (en) * 2002-05-15 2005-09-13 Pharmacopeia Drug Discovery, Inc. Methods for measuring kinase activity
CN1671726A (zh) * 2002-05-31 2005-09-21 詹森药业有限公司 3－脱克拉定糖基－6－o－氨基甲酰基和6－o－烷氧基羰基大环内酯抗菌药
US7524974B2 (en) * 2002-07-08 2009-04-28 Tetsuo Nagano Fluorescent probe
CA2497991A1 (en) * 2002-09-09 2004-03-18 Applera Corporation Fluorescent enzyme assay methods and compositions
WO2004040296A1 (ja) * 2002-10-16 2004-05-13 Daiichi Pure Chemicals Co., Ltd. パーオキシナイトライト測定用試薬
WO2004072613A2 (en) * 2003-02-07 2004-08-26 Board Of Regents, The University Of Texas System Multi-shell microspheres with integrated chomatographic and detection layers for use in array sensors
US7696245B2 (en) * 2003-03-28 2010-04-13 Sekisui Medical Co., Ltd. Fluorescent probe for zinc
WO2004097371A2 (en) * 2003-04-25 2004-11-11 Board Of Regents, The University Of Texas System System and method for the detection of analytes
US9317922B2 (en) 2003-05-16 2016-04-19 Board Of Regents The University Of Texas System Image and part recognition technology
WO2004104922A2 (en) * 2003-05-16 2004-12-02 Board Of Regents, The University Of Texas System Image and part recognition technology
WO2005017191A2 (en) * 2003-06-23 2005-02-24 Nlcpharma, Inc. Enzymatic diagnostic test for sars and other viral diseases
PT1678209E (pt) 2003-10-07 2011-06-30 Yeda Res & Dev Anticorpos para a nik, a sua preparação e a sua utilização
CA2541765A1 (en) 2003-10-10 2004-10-01 Promega Corporation Luciferase biosensor
EP1689764B1 (de) * 2003-11-19 2013-01-02 AlleLogic Biosciences Corporation Mit mehreren fluorophoren markierte oligonukleotide
US20050244891A1 (en) * 2003-11-26 2005-11-03 Applera Corporation Ligand-containing micelles and uses thereof
US20050214890A1 (en) * 2003-11-26 2005-09-29 Zhiqun Tan Novel "Cleave-N-Read" system for protease activity assay and methods of use thereof
EP1690089A1 (de) * 2003-11-26 2006-08-16 Applera Corporation Fluorogene homogene bindungstestverfahren und zusammensetzungen
US20050124008A1 (en) * 2003-12-05 2005-06-09 Kauvar Lawrence M. Homogeneous competition assays
JP2005194244A (ja) * 2004-01-09 2005-07-21 Shigenobu Yano 亜鉛イオン蛍光センサー
EP1704257A2 (de) * 2004-01-16 2006-09-27 Applera Corporation Fluorogene kinase-assays sowie substrate für kinasen und phosphatasen
US8105849B2 (en) * 2004-02-27 2012-01-31 Board Of Regents, The University Of Texas System Integration of fluids and reagents into self-contained cartridges containing sensor elements
US8101431B2 (en) * 2004-02-27 2012-01-24 Board Of Regents, The University Of Texas System Integration of fluids and reagents into self-contained cartridges containing sensor elements and reagent delivery systems
EP1598428A1 (de) 2004-05-18 2005-11-23 Georg Dewald Verfahren und Kits zum Nachweis von erblichen angioedema typ III
US20060035302A1 (en) * 2004-06-21 2006-02-16 Applera Corporation Kinase substrates with multiple phosphorylation sites
US20060121553A1 (en) * 2004-10-29 2006-06-08 Applera Corporation Fluorogenic enzyme assay methods, kits and compositions using charge-balancers
US20060177918A1 (en) * 2004-12-30 2006-08-10 Applera Corporation Compositions, methods and kits for real-time enzyme assays using charged molecules
US20090023200A1 (en) * 2005-01-13 2009-01-22 Kyushu Institute Of Technology Particle for detecting enzyme activity, method for detecting the enzyme activity and enzyme activity detection device by the use thereof
US7601498B2 (en) * 2005-03-17 2009-10-13 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection and associated technology
US7776567B2 (en) * 2005-03-17 2010-08-17 Biotium, Inc. Dimeric and trimeric nucleic acid dyes, and associated systems and methods
WO2007053186A2 (en) 2005-05-31 2007-05-10 Labnow, Inc. Methods and compositions related to determination and use of white blood cell counts
EP1899450A4 (de) * 2005-06-24 2010-03-24 Univ Texas Systeme und verfahren einschliesslich in sich geschlossener kassetten mit nachweissystemen und flüssigkeitszufuhrsystemen
US8227621B2 (en) * 2005-06-30 2012-07-24 Li-Cor, Inc. Cyanine dyes and methods of use
WO2007005666A2 (en) * 2005-07-01 2007-01-11 Board Of Regents, The University Of Texas System System and method of analyte detection using differential receptors
US20070073039A1 (en) * 2005-09-29 2007-03-29 Chisari Francis V Peptides that inhibit viral infections
DE102005051978A1 (de) * 2005-10-31 2007-05-10 Forschungszentrum Borstel Zentrum für Medizin und Biowissenschaften Verfahren zur Bestimmung der Spaltbarkeit von Substraten
CA2634987C (en) * 2006-01-17 2017-01-03 Somalogic, Inc. Multiplexed analyses of test samples
EP2327768B1 (de) 2006-04-03 2015-09-09 Promega Corporation Permutierte und nichtpermutierte Luciferase-Biosensoren
US8077974B2 (en) 2006-07-28 2011-12-13 Hewlett-Packard Development Company, L.P. Compact stylus-based input technique for indic scripts
AT504443B1 (de) * 2006-10-19 2008-11-15 Apeiron Biolog Forschungs Und Verfahren zur bestimmung der aktivität von ace2
US7855054B2 (en) * 2007-01-16 2010-12-21 Somalogic, Inc. Multiplexed analyses of test samples
US20080300798A1 (en) * 2007-04-16 2008-12-04 Mcdevitt John T Cardibioindex/cardibioscore and utility of salivary proteome in cardiovascular diagnostics
US20080269065A1 (en) * 2007-04-30 2008-10-30 Syntrix Biosystems, Inc. Conformationally Constrained Analytical Probes
US8759019B2 (en) 2007-07-06 2014-06-24 Papst Licensing Gmbh & Co. Kg Method for measuring the activity of proteases
US8906700B2 (en) 2007-11-06 2014-12-09 Ambergen, Inc. Methods and compositions for phototransfer
US20090124875A1 (en) * 2007-11-14 2009-05-14 Cardiac Pacemakers, Inc. Implantable Creatinine Sensor and Related Methods
WO2009078970A1 (en) * 2007-12-14 2009-06-25 Biotium, Inc. Fluorescent compounds
EP2077119A1 (de) * 2007-12-21 2009-07-08 Apeiron Biologics Forschungs- und Entwicklungsgesellschaft M.B.H. Behandlung von Fibrosen und Lebererkrankungen
WO2009112791A1 (en) * 2008-03-14 2009-09-17 Assaymetrics Limited Fluorogenic peptides and their method of production
WO2009117539A2 (en) * 2008-03-19 2009-09-24 Abbott Laboratories Bioluminescent construct
WO2009142735A2 (en) 2008-05-19 2009-11-26 Promega Corporation LUCIFERASE BIOSENSORS FOR cAMP
WO2010006616A2 (en) * 2008-07-14 2010-01-21 Chemometec A/S Method and kit for examination of cells using n-(9-acridinyl)maleimide (nam) or using 7-diethylamino-3-((4'-(iodoacetyl)amino)phenyl)-4-methylcoumarin (cpi)
US9128097B2 (en) 2008-07-14 2015-09-08 Chemometec A/S Method and kit for assessing viable cells
US9186128B2 (en) 2008-10-01 2015-11-17 Covidien Lp Needle biopsy device
US8968210B2 (en) 2008-10-01 2015-03-03 Covidien LLP Device for needle biopsy with integrated needle protection
US9332973B2 (en) 2008-10-01 2016-05-10 Covidien Lp Needle biopsy device with exchangeable needle and integrated needle protection
US11298113B2 (en) 2008-10-01 2022-04-12 Covidien Lp Device for needle biopsy with integrated needle protection
US9782565B2 (en) 2008-10-01 2017-10-10 Covidien Lp Endoscopic ultrasound-guided biliary access system
WO2010058396A1 (en) 2008-11-19 2010-05-27 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. A cd44vra antibody and diagnostic and therapeutic methods using same
JP5798487B2 (ja) * 2008-11-20 2015-10-21 ネーデルランドセ・オルガニサティ・フォール・トゥーヘパスト−ナトゥールウェテンスハッペライク・オンデルズーク・テーエヌオー Ｆｒｅｔに基づく病原体の迅速診断
DE102009004371A1 (de) 2009-01-08 2010-07-15 Papst Licensing Gmbh & Co. Kg Vorrichtung und Verfahren zum Messen der Aktivität von Enzymen nach Inhibitorentzug
US8673267B2 (en) 2009-03-02 2014-03-18 Massachusetts Institute Of Technology Methods and products for in vivo enzyme profiling
EP2435072A2 (de) 2009-05-27 2012-04-04 Yeda Research and Development Co. Ltd. Proteasom-hemmer und ihre verwendungen
US8658434B2 (en) * 2009-10-28 2014-02-25 Biotium, Inc. Fluorescent pyrene compounds
MX2012006877A (es) 2009-12-18 2012-08-31 Idenix Pharmaceuticals Inc Inhibidores de virus de hepatitis c de arileno o heteroarileno 5, 5 - fusionado.
US8877437B1 (en) 2009-12-23 2014-11-04 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection
CA2787311C (en) 2010-01-27 2017-08-15 Yeda Research And Development Co. Ltd. Antibodies that inhibit metalloproteins
WO2011107994A1 (en) 2010-03-04 2011-09-09 Yeda Research And Development Co. Ltd. Methods of measuring protein stability
US8735559B2 (en) 2010-05-11 2014-05-27 Promega Corporation Mutant protease biosensors with enhanced detection characteristics
US9290794B2 (en) * 2010-05-11 2016-03-22 Promega Corporation Mutant protease biosensors with enhanced detection characteristics
WO2011151833A1 (en) 2010-06-03 2011-12-08 Ramot At Tel-Aviv University Ltd. Methods of treating diabetes and compositions capable of same
CA2805478A1 (en) 2010-07-15 2012-01-19 Technion Research & Development Foundation Ltd. Isolated high affinity entities with t-cell receptor like specificity towards native complexes of mhc class ii and diabetes-associated autoantigenic peptides
US9695410B2 (en) 2010-07-15 2017-07-04 Technion Research & Development Foundation Limited Isolated high affinity entities with T-cell receptor like specificity towards native complexes of MHC class II and glutamic acid decarboxylase (GAD) autoantigenic peptides
WO2012056455A1 (en) 2010-10-28 2012-05-03 Yeda Research And Development Co. Ltd. Methods of generating antibodies to metalloenzymes
IN2013MN01438A (de) 2011-03-17 2015-06-12 Univ Ramot
WO2013025261A2 (en) * 2011-04-06 2013-02-21 Georgia Tech Research Corporation Fluorogenic peptide probes and assays
US8858929B2 (en) 2012-01-04 2014-10-14 Technion Research & Development Foundation Limited Optically sensitive cell network
EP3483184A1 (de) 2012-03-07 2019-05-15 Yeda Research and Development Co. Ltd Zusammensetzungen zur hemmung der quiescinsulfhydryl (qsox1)-oxidase und verwendungen davon
EP2906954B1 (de) 2012-10-09 2018-04-11 Ramot at Tel-Aviv University Ltd. Verfahren und kits zur prognose von krebs unter verwendung von löslichem mortalin im blut
JP6847660B2 (ja) 2013-06-07 2021-03-24 マサチューセッツ インスティテュート オブ テクノロジー リガンドをコードする合成バイオマーカーのアフィニティベースの検出
WO2015037009A1 (en) 2013-09-16 2015-03-19 Plexicure Ltd. Isolated proteins capable of binding plexin-a4 and methods of producing and using same
CN106573976B (zh) 2014-02-06 2020-05-05 耶达研究及发展有限公司 抗cd84抗体、包含所述抗体的组合物及其用途
US10883994B2 (en) 2014-06-25 2021-01-05 Tel Hashomer Medical Research Infrastructure And Services Ltd. Identification of cancer stem cells markers and use of same for diagnosis and treatment
WO2016038609A1 (en) 2014-09-08 2016-03-17 Yeda Research And Development Co. Ltd. Anti-her3 antibodies and uses of same
WO2016164596A2 (en) * 2015-04-07 2016-10-13 Novozymes A/S Methods for selecting enzymes having lipase activity
US10716500B2 (en) 2015-06-29 2020-07-21 Cardiac Pacemakers, Inc. Systems and methods for normalization of chemical sensor data based on fluid state changes
WO2017072757A1 (en) 2015-10-25 2017-05-04 Yeda Research And Development Co. Ltd. Antibodies targeting quiescin sulfhydryl oxidase (qsox1) and uses of same
EP3403091B1 (de) 2016-01-11 2021-12-22 Technion Research & Development Foundation Limited Verfahren zur bestimmung der prognose von sepsis und behandlung davon
WO2017175228A1 (en) 2016-04-06 2017-10-12 Technion Research & Development Foundation Limited Infiltrating immune cell proportions predict anti-tnf response in colon biopsies
EP3440013A4 (de) 2016-04-08 2021-03-17 Massachusetts Institute of Technology Verfahren zur spezifischen profilierung der protease-aktivität an lymphknoten
WO2017193070A1 (en) 2016-05-05 2017-11-09 Massachusetts Institute Of Technology Methods and uses for remotely triggered protease activity measurements
CA3059358A1 (en) 2017-04-07 2018-10-11 Massachusetts Institute Of Technology Methods to spatially profile protease activity in tissue and sections
WO2019155465A1 (en) 2018-02-08 2019-08-15 Yeda Research And Development Co. Ltd. Methods of identifying and using agents for treating diseases associated with intestinal barrier dysfunction
EP3759493A1 (de) 2018-02-28 2021-01-06 The Medical Research, Infrastructure and Health Services Fund of the Tel Aviv Medical Center Verfahren zur diagnose und behandlung von blasenkrebs
WO2019173332A1 (en) 2018-03-05 2019-09-12 Massachusetts Institute Of Technology Inhalable nanosensors with volatile reporters and uses thereof
IL261156A (en) 2018-08-14 2020-02-27 Fass Deborah Chimeric quiescin sulfhydryl oxidase (qsox1) antibodies and uses of same
WO2020053808A1 (en) 2018-09-12 2020-03-19 Georg Dewald Method of diagnosing vasoregulatory disorders
US12173349B2 (en) 2018-09-25 2024-12-24 Massachusetts Institute Of Technology Lung protease nanosensors and uses thereof
JP2022515728A (ja) * 2018-12-20 2022-02-22 武田薬品工業株式会社 好中球エラスターゼの新規な活性ベースのプローブおよびその使用
US11835522B2 (en) 2019-01-17 2023-12-05 Massachusetts Institute Of Technology Sensors for detecting and imaging of cancer metastasis
IL264768A (en) 2019-02-10 2020-08-31 Sagi Irit Anti-matrix metalloproteinase 7 (mmp-7) inhibitory antibody and uses thereof
US12509512B2 (en) 2019-06-27 2025-12-30 Ramot At Tel-Aviv University Ltd. Semaphorin 3A antibodies and uses thereof
WO2021149040A1 (en) 2020-01-22 2021-07-29 Ramot At Tel-Aviv University Ltd. Anti-ide antibodies and uses of same
US11603552B2 (en) 2020-07-20 2023-03-14 Mesa Photonics, LLC Method for pathogen identification
CN113588768B (zh) * 2021-05-18 2022-07-05 国家卫生健康委科学技术研究所 一种以分子图像方式定量组织内内源性代谢物的质谱方法
EP4584297A1 (de) 2022-09-11 2025-07-16 Yeda Research and Development Co. Ltd Anti-klk4-antikörper und verwendungen davon
WO2026009227A1 (en) 2024-07-04 2026-01-08 Yeda Research And Development Co. Ltd. Compositions for downregulating zeb2 in macrophages and uses thereof

Family Cites Families (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4557862A (en) * 1983-10-28 1985-12-10 University Patents, Inc. Rhodamine derivatives as fluorogenic substrates for proteinases
US4708929A (en) * 1984-10-29 1987-11-24 Microgenics Corporation Methods for protein binding enzyme complementation assays
US5110801A (en) 1984-08-13 1992-05-05 Leveen Harry H Treatment of acne
FR2570364B1 (fr) * 1984-09-18 1991-09-20 Air Liquide Procede et dispositif de lubrification d'une empreinte de moulage, et leur application a une machine de fabrication de bouteilles en verre
US4897444A (en) * 1985-05-31 1990-01-30 The Research Foundation Of The State University Of New York Immobilized fluorogenic substrates for enzymes; and processes for their preparation
US4780421A (en) * 1986-04-03 1988-10-25 Sclavo Inc. Cleavable labels for use in binding assays
US5118801A (en) 1988-09-30 1992-06-02 The Public Health Research Institute Nucleic acid process containing improved molecular switch
US5212298A (en) * 1989-08-16 1993-05-18 Monsanto Company Method for producing synthetic N-linked glycoconjugates
US5011910A (en) 1989-12-28 1991-04-30 Washington University Reagent and method for determining activity of retroviral protease
US5539082A (en) 1993-04-26 1996-07-23 Nielsen; Peter E. Peptide nucleic acids
WO1994016106A1 (en) 1993-01-15 1994-07-21 The Public Health Research Institute Of The City Of New York, Inc. Diagnostic assays and kits for rna using rna binary probes and an rna-directed rna ligase
DE69430665T2 (de) 1993-01-15 2002-11-21 The Public Health Research Institute Of The City Of New York, Inc. Empfindlicher nukleinsäure-sandwichhybridisierungs-assay und kits
AU6088094A (en) 1993-01-15 1994-08-15 General Hospital Corporation, The Rna assays using rna binary probes and ribozyme ligase
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
JP2664878B2 (ja) 1994-01-31 1997-10-22 インターナショナル・ビジネス・マシーンズ・コーポレイション 半導体チップパッケージおよびその製造方法
US5506115A (en) 1994-04-29 1996-04-09 G. D. Searle & Co. Reagent and method for determining activity of herpes protease
US5723288A (en) 1994-05-06 1998-03-03 The University Of North Carolina At Chapel Hill Method of fluorescent detection of nucleic acids and cytoskeleton elements using bis-dicationic aryl furans, and kits useful therefor
US5681821A (en) * 1994-10-18 1997-10-28 Georgia Tech Research Corp. Fluorescent 1-peptidylaminoalkanephosphonate derivatives
US5605809A (en) * 1994-10-28 1997-02-25 Oncoimmunin, Inc. Compositions for the detection of proteases in biological samples and methods of use thereof
US5698411A (en) 1995-05-18 1997-12-16 Coulter Corporation Method for determining activity of enzymes in metabolically active whole cells
US5871946A (en) 1995-05-18 1999-02-16 Coulter Corporation Method for determining activity of enzymes in metabolically active whole cells
US5776720A (en) 1995-05-18 1998-07-07 Coulter Corporation Assay reagent
US5733719A (en) 1995-05-18 1998-03-31 Coulter Corporation Method of making an assay compound
US5804395A (en) 1995-12-01 1998-09-08 The United States Of America As Represented By The Secretary Of The Navy Fluorescence polarization assays of enzymes and substrates therefore
WO1998010096A1 (en) 1996-04-12 1998-03-12 The Public Health Research Institute Of The City Of New York, Inc. Detection probes, kits and assays
AU713667B2 (en) 1996-04-12 1999-12-09 Phri Properties, Inc. Detection probes, kits and assays
US5925558A (en) 1996-07-16 1999-07-20 The Regents Of The University Of California Assays for protein kinases using fluorescent protein substrates
US5912137A (en) 1996-07-16 1999-06-15 The Regents Of The University Of California Assays for protein kinases using fluorescent
US5714392A (en) * 1996-07-26 1998-02-03 Advanced Micro Devices, Inc. Rapid thermal anneal system and method including improved temperature sensing and monitoring
US6037103A (en) 1996-12-11 2000-03-14 Nitto Denko Corporation Method for forming hole in printed board
WO2001018238A1 (en) 1999-09-10 2001-03-15 Oncoimmunin, Inc. Compositions for the detection of enzyme activity in biological samples and methods of use thereof
US6893868B2 (en) * 1997-02-20 2005-05-17 Onco Immunin, Inc. Homo-doubly labeled compositions for the detection of enzyme activity in biological samples
US7312302B2 (en) * 1997-02-20 2007-12-25 Oncolmmunin, Inc. Compositions for the detection of enzyme activity in biological samples and methods of use thereof
US6037137A (en) 1997-02-20 2000-03-14 Oncoimmunin, Inc. Fluorogenic peptides for the detection of protease activity
US5998204A (en) 1997-03-14 1999-12-07 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
US6037130A (en) 1998-07-28 2000-03-14 The Public Health Institute Of The City Of New York, Inc. Wavelength-shifting probes and primers and their use in assays and kits
US6410255B1 (en) 1999-05-05 2002-06-25 Aurora Biosciences Corporation Optical probes and assays
WO2000071740A1 (en) 1999-05-24 2000-11-30 The Public Health Research Institute Of The City Of New York, Inc. High specificity hairpin antisense oligonucleotides
US6277607B1 (en) 1999-05-24 2001-08-21 Sanjay Tyagi High specificity primers, amplification methods and kits
DE60030145T2 (de) 1999-10-22 2008-01-10 PHRI Properties, Inc., Newark Nachweisverfahren für kurze sequenzvarianten

Also Published As

Publication number Publication date
JP2008167757A (ja) 2008-07-24
EP0988394B1 (de) 2009-12-30
EP0988394A1 (de) 2000-03-29
JP4298796B2 (ja) 2009-07-22
AU745148B2 (en) 2002-03-14
CA2280811A1 (en) 1998-08-27
US20080199898A1 (en) 2008-08-21
WO1998037226A1 (en) 1998-08-27
AU6656798A (en) 1998-09-09
EP0988394A4 (de) 2003-04-23
US6936687B1 (en) 2005-08-30
DE69841419D1 (de) 2010-02-11
CA2280811C (en) 2015-11-03
JP2001514492A (ja) 2001-09-11
US6037137A (en) 2000-03-14
US7879574B2 (en) 2011-02-01

Similar Documents

Publication Publication Date Title
ATE453722T1 (de) Mittel zur detektion von enzymaktivitäten in biologischen proben und verfahren zu dessen gebrauch
EP0873417A4 (de) Zusammensetzungen für den nachweis von proteasen in biologischen proben und verfahren zu deren verwendung
ATE393228T1 (de) Fusionsproteine und deren verwendung zur messung von protease-aktivität
EP0938320A4 (de) Methoden, die aus plasma oder serum extrahiertes extrazellulares rna zur diagnoseüberwachung und evulation von krebs
DE60331778D1 (de) Metalloproteaseaktivierung von myostatin und verfahren zur modulation der myostatinaktivität
DE69637047D1 (de) Unabhängiges gerät zur extraktion, amplifikation und nachweis von nukleinsäuren
DK1042664T3 (da) Fremgangsmåde til påvisning af reaktioner ved hjælp af koincidensanalyse
DK1198589T3 (da) Tenascin-C-nukleinsyre-ligander
ATE393237T1 (de) Mittel und verfahren zur in-vitro-bestimmung von camp
WO2003021226A3 (en) Peptide biomarker and method of identification
ATE411339T1 (de) Spezifischer nachweis von proteolytischen enzymen mittels eines hybrid-proteins
Sato et al. Novel fluorescent substrates for detection of trypsin activity and inhibitor screening by self-quenching
DE69630142D1 (de) Verfahren zur identifizierung metastatischer sequenzen
ATE513851T1 (de) Menschliches gen zur fruchtbarkeit entscheidend
WO2004033397A3 (en) Activity-based probes, and methods of their preparation and use
ATE451617T1 (de) Verfahren zur erkennung eines kolonkrebsmarkers
DE50107766D1 (de) Zweifarbiges fluorimetrisches proteaseassay
ATE290607T1 (de) Floureszenzintensitäts-test für eine protein- oder peptidbindung an nukleinsäuren
Carvalho et al. A highly selective assay for neutral endopeptidase based on the cleavage of a fluorogenic substrate related to leu-enkephalin
ES552374A0 (es) Un procedimiento para determinar la actividad de una hidrolasa macromolecular
da Silva Gomes et al. A fluorimetric method for the determination of pepsin activity
ATE315107T1 (de) Verfahren und kit zur früherkennung von krebs
Yan et al. Development of intramolecularly quenched fluorescent peptides as substrates of angiotensin-converting enzyme 2
Budič et al. A quantitative technique for determining proteases and their substrate specificities and pH optima in crude enzyme extracts
Katunuma et al. Double-layer fluorescent zymography for processing protease detection

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties