ATE456669T1 - Verfahren zur quantifizierung von nukleinsäuren - Google Patents

Verfahren zur quantifizierung von nukleinsäuren

Info

Publication number
ATE456669T1
ATE456669T1 AT03028474T AT03028474T ATE456669T1 AT E456669 T1 ATE456669 T1 AT E456669T1 AT 03028474 T AT03028474 T AT 03028474T AT 03028474 T AT03028474 T AT 03028474T AT E456669 T1 ATE456669 T1 AT E456669T1
Authority
AT
Austria
Prior art keywords
nucleic acid
target nucleic
nucleic acids
quantifying
sequence
Prior art date
Application number
AT03028474T
Other languages
English (en)
Inventor
Yoshihide Iwaki
Yoshihiko Makino
Original Assignee
Fujifilm Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fujifilm Corp filed Critical Fujifilm Corp
Application granted granted Critical
Publication of ATE456669T1 publication Critical patent/ATE456669T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT03028474T 2002-12-12 2003-12-12 Verfahren zur quantifizierung von nukleinsäuren ATE456669T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP2002360914A JP4185763B2 (ja) 2002-12-12 2002-12-12 ターゲット核酸の定量方法

Publications (1)

Publication Number Publication Date
ATE456669T1 true ATE456669T1 (de) 2010-02-15

Family

ID=32501017

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03028474T ATE456669T1 (de) 2002-12-12 2003-12-12 Verfahren zur quantifizierung von nukleinsäuren

Country Status (5)

Country Link
US (1) US20050032080A1 (de)
EP (1) EP1435394B1 (de)
JP (1) JP4185763B2 (de)
AT (1) ATE456669T1 (de)
DE (1) DE60331132D1 (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2270203A1 (de) * 2009-06-29 2011-01-05 AIT Austrian Institute of Technology GmbH Oligonukleotid-Hybridisierungsverfahren
AU2012249531B2 (en) * 2011-04-29 2017-06-29 Sequenom, Inc. Quantification of a minority nucleic acid species
EP3736344A1 (de) 2014-03-13 2020-11-11 Sequenom, Inc. Verfahren und prozesse zur nichtinvasiven beurteilung genetischer variationen
WO2017173035A1 (en) * 2016-04-01 2017-10-05 Chromacode Inc. Competitive probes for engineering signal generation
EP4692778A1 (de) 2023-03-31 2026-02-11 Denka Company Limited Verfahren zur detektion von pyrophosphorsäure

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
JPH0833393B2 (ja) * 1988-03-14 1996-03-29 富士写真フイルム株式会社 乾式分析要素
FR2674254B1 (fr) * 1991-03-20 1995-10-06 Univ Reims Champagne Ardenne Detection non radioactive de la presence d'un acide nucleique determine dans un echantillon biologique.
JP2882170B2 (ja) * 1992-03-19 1999-04-12 日産自動車株式会社 能動型騒音制御装置
JP3167508B2 (ja) * 1993-06-15 2001-05-21 富士写真フイルム株式会社 無機燐定量用試薬及び乾式分析要素
EP0630974A3 (de) * 1993-06-25 1995-11-15 Clinical Diagnostic Syst Verfahren und Testkit zum Nachweis von anorganischen Orthophosphat bei der Amplifizierung von Zielnukleinsäuren.
WO1995025180A1 (en) * 1994-03-16 1995-09-21 Gen-Probe Incorporated Isothermal strand displacement nucleic acid amplification
US6057134A (en) * 1996-10-07 2000-05-02 Ambion, Inc. Modulating the efficiency of nucleic acid amplification reactions with 3' modified oligonucleotides
GB2324865B (en) * 1997-05-02 2001-05-02 William Joseph Harris Materials and methods for detecting amplified nucleic acids
AU7813698A (en) * 1997-06-06 1998-12-21 Nexstar Pharmaceuticals, Inc. Nucleic acid detection
WO2002018616A1 (en) 2000-09-01 2002-03-07 Hitachi Chemical Co., Ltd. Adjusting the efficiency of nucleic acid template amplification by attenuated pcr with template-mimic oligonucleotides

Also Published As

Publication number Publication date
EP1435394A1 (de) 2004-07-07
US20050032080A1 (en) 2005-02-10
DE60331132D1 (de) 2010-03-18
JP4185763B2 (ja) 2008-11-26
JP2004187603A (ja) 2004-07-08
EP1435394B1 (de) 2010-01-27

Similar Documents

Publication Publication Date Title
ATE480644T1 (de) Verfahren zur quantifizierung kleiner rna- moleküle
ATE550441T1 (de) Verfahren, zusammensetzungen und kits zur isothermischen amplifikation von nukleinsäuren
ATE360093T1 (de) Reaktionsgemisch und verfahren für rt-pcr mittels einer homopolymeren nukleinsäure
DE69837913D1 (de) Verfahren zur vervielfältigung von nukleinsäure
DE602004021235D1 (de) Verfahren, kits und reagenzien zur nukleinsäuresequenzierung
BR9801878B1 (pt) oligonucleotìdeos, método para amplificação de uma seqüência alvo de ácido nucléico e kit para realizar uma reação de amplificação de ácido nucléico.
ATE557083T1 (de) Thermostabiler oder thermoactiver dna-polymerase mit abgeschwächter 3'-5' exonukleaseaktivität
DK1427852T3 (da) Enkelnukleotidamplikation og -detektion med polymerase
DE602004022253D1 (de) Verfahren zur amplifikation und sequenzierung von nukleinsäuren
WO2005089508A3 (en) Dna sequence detection by limited primer extension
DK1026261T3 (da) Forögelse af specificiteten af nukleinsyreamplifikation med bærernukleinsyre
DE112006002611A5 (de) Rna-abhängige rna-polymerase, verfahren und kits zur amplifikation und / oder markierung von rna
DE60309817D1 (de) Dopplezweck -Primer und -Proben für verbesserte Hybridisierungsassays durch Zerstörung von Sekundärstrukturen
ATE458044T1 (de) Verfahren zur nukleinsäureamplifikation
ATE455866T1 (de) Verfahren zur unspezifischen amplifizierung nukleinsäuren mit niedriger komplexität
ATE497022T1 (de) Verfahren zur amplifikation von nukleinsäure
ATE456669T1 (de) Verfahren zur quantifizierung von nukleinsäuren
ATE526418T1 (de) Verbesserte zusammensetzungen zur in-vitro- amplifikation von nukleinsäuren
EP1772523A3 (de) Polymerase-Kettenreaktionsverfahren unter Einsatz einer DNA-Polymerase mit Proofreading-Eigenschaften
ATE530646T1 (de) Verfahren zum nachweis und zur quantifizierung von endogener weizen-dna-sequenz
ATE347618T1 (de) Verfahren zur amplifikation von nukleinsäuren, das mittel zur strangtrennung und universelle sequenzmarker bereitstellt
ATE420975T1 (de) Methode zur linearen nicht-selektiven amplifikation von nukleinsäuren
DE50214183D1 (de) Verfahren zum nachweis von in einer polymerase-kettenreaktion amplifizierten nukleinsäuremolekülen
WO2004020975A3 (en) Method for detecting microorganisms using pcr amplicons and microsphere agglutination

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties