ATE456672T1 - Zusammensetzungen und verfahren zur verhinderung des mitschleppens einer kontamination in nukleinsäure-amplifikationsreaktionen - Google Patents
Zusammensetzungen und verfahren zur verhinderung des mitschleppens einer kontamination in nukleinsäure-amplifikationsreaktionenInfo
- Publication number
- ATE456672T1 ATE456672T1 AT05762161T AT05762161T ATE456672T1 AT E456672 T1 ATE456672 T1 AT E456672T1 AT 05762161 T AT05762161 T AT 05762161T AT 05762161 T AT05762161 T AT 05762161T AT E456672 T1 ATE456672 T1 AT E456672T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acid
- contaminating
- methods
- amplificate
- carry
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 5
- 230000003321 amplification Effects 0.000 title abstract 4
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 4
- 238000006243 chemical reaction Methods 0.000 title abstract 3
- 108020004707 nucleic acids Proteins 0.000 title abstract 3
- 150000007523 nucleic acids Chemical class 0.000 title abstract 3
- 102000039446 nucleic acids Human genes 0.000 title abstract 3
- 238000011109 contamination Methods 0.000 title 1
- 239000000203 mixture Substances 0.000 title 1
- 102000006382 Ribonucleases Human genes 0.000 abstract 2
- 108010083644 Ribonucleases Proteins 0.000 abstract 2
- 239000000356 contaminant Substances 0.000 abstract 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 abstract 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 108091028664 Ribonucleotide Proteins 0.000 abstract 1
- 230000015556 catabolic process Effects 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
- 238000006731 degradation reaction Methods 0.000 abstract 1
- 230000001419 dependent effect Effects 0.000 abstract 1
- 230000029087 digestion Effects 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 239000002336 ribonucleotide Substances 0.000 abstract 1
- 125000002652 ribonucleotide group Chemical group 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US58052904P | 2004-06-17 | 2004-06-17 | |
| US58063804P | 2004-06-17 | 2004-06-17 | |
| US58064404P | 2004-06-17 | 2004-06-17 | |
| PCT/US2005/021525 WO2006009870A2 (en) | 2004-06-17 | 2005-06-17 | Compositions and methods for preventing carry-over contamination in nucleic acid amplification reactions |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE456672T1 true ATE456672T1 (de) | 2010-02-15 |
Family
ID=34972771
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT05762161T ATE456672T1 (de) | 2004-06-17 | 2005-06-17 | Zusammensetzungen und verfahren zur verhinderung des mitschleppens einer kontamination in nukleinsäure-amplifikationsreaktionen |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20080311627A1 (de) |
| EP (1) | EP1781813B1 (de) |
| AT (1) | ATE456672T1 (de) |
| DE (1) | DE602005019174D1 (de) |
| WO (1) | WO2006009870A2 (de) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1632578A1 (de) * | 2004-09-03 | 2006-03-08 | Roche Diagnostics GmbH | Methode zur Dekontamination der DNA |
| WO2007141034A1 (en) * | 2006-06-08 | 2007-12-13 | Epigenomics Ag | Carry-over protection in enzyme-based dna amplification systems targeting methylation analysis |
| EP2480685B1 (de) | 2009-09-23 | 2013-08-28 | Koninklijke Philips Electronics N.V. | Verfahren zur überwachung der bisulfitvermittelten umwandlung von dna |
| ES2449665B2 (es) * | 2012-09-19 | 2014-07-17 | Servizo Galego De Saúde-Conselleria De Sanidade, Xunta De Galicia | Procedimiento para el diagnóstico clínico de una enfermedad infecciosa basada en el empleo de la PCR cuantitativa, oligonucleótidos marcados de 8 a 9 nucleótidos de longitud y la UNG del Bacalao del Atlántico (Gadus morhua). |
| CA2892043C (en) | 2012-11-21 | 2022-01-11 | Courtagen Life Sciences Inc. | A method of removing amplicons of a non-target nucleic acid having one or more methylated cytosines from a sample |
| WO2019200016A1 (en) | 2018-04-10 | 2019-10-17 | President And Fellows Of Harvard College | Aav vectors encoding clarin-1 or gjb2 and uses thereof |
| CN112301102B (zh) * | 2019-07-31 | 2024-06-25 | 华南师范大学 | 一种高特异性的环介导等温扩增方法及其应用 |
| EP4107264A4 (de) * | 2020-02-21 | 2024-06-05 | Mission Bio, Inc. | Verbesserter nachweis von zielnukleinsäuren durch entfernung von dna-rna-kreuzkontamination |
| MX2023002978A (es) * | 2020-09-14 | 2023-06-01 | Harvard College | Virus adenoasociado recombinante (raav) que codifica la union gap beta 2 (gjb2) y sus usos. |
| US12129287B2 (en) | 2020-09-14 | 2024-10-29 | President And Fellows Of Harvard College | Recombinant adeno associated virus encoding clarin-1 and uses thereof |
| CN114317799B (zh) * | 2021-12-21 | 2023-10-13 | 中国中医科学院中药研究所 | 一种鉴定当归及常见当归混伪品的特异性引物对及其应用 |
| CN116773291B (zh) * | 2023-03-31 | 2024-04-19 | 艾普拜生物科技(苏州)有限公司 | 一种石蜡切片dna预处理试剂及方法 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683195A (en) * | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| HU218095B (hu) * | 1990-05-01 | 2000-05-28 | Amgen Inc. | Eljárás átvitt szennyeződések csökkentésére, amplifikációs eljárásokban |
| ES2128323T3 (es) * | 1990-07-24 | 1999-05-16 | Hoffmann La Roche | Reduccion de la amplificacion no-especifica durante la amplificacion in vitro del acido nucleico empleando bases de acido nucleico modificadas. |
| US5786146A (en) * | 1996-06-03 | 1998-07-28 | The Johns Hopkins University School Of Medicine | Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids |
| CZ293215B6 (cs) * | 1996-08-06 | 2004-03-17 | F. Hoffmann-La Roche Ag | Rekombinantní tepelně stálá DNA polymeráza, způsob její přípravy a prostředek, který ji obsahuje |
| GB9716664D0 (en) * | 1997-08-06 | 1997-10-15 | Norwegian Inst Of Fisheries & | A method of removing nucleic acid contamination reactions |
| US6605432B1 (en) * | 1999-02-05 | 2003-08-12 | Curators Of The University Of Missouri | High-throughput methods for detecting DNA methylation |
| US7214485B2 (en) * | 2000-08-25 | 2007-05-08 | Lovelace Respiratory Research Institute | Nested methylation-specific polymerase chain reaction cancer detection method |
| EP1438428A4 (de) * | 2001-09-14 | 2005-11-16 | Univ Queensland | Nachweis der methylierung von dna |
| AUPS064202A0 (en) * | 2002-02-20 | 2002-03-14 | Biomolecular Research Solutions Pty Ltd | Improved method for the analysis of nucleic acid samples |
| DE102004002257B4 (de) * | 2004-01-09 | 2006-11-09 | Epigenomics Ag | Verfahren zur Untersuchung von Cytosin-Methylierungen in DNA mit Hilfe von DNA-Reparaturenzymen |
-
2005
- 2005-06-17 AT AT05762161T patent/ATE456672T1/de not_active IP Right Cessation
- 2005-06-17 WO PCT/US2005/021525 patent/WO2006009870A2/en not_active Ceased
- 2005-06-17 US US11/663,420 patent/US20080311627A1/en not_active Abandoned
- 2005-06-17 EP EP05762161A patent/EP1781813B1/de not_active Expired - Lifetime
- 2005-06-17 DE DE602005019174T patent/DE602005019174D1/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP1781813A2 (de) | 2007-05-09 |
| EP1781813B1 (de) | 2010-01-27 |
| US20080311627A1 (en) | 2008-12-18 |
| DE602005019174D1 (de) | 2010-03-18 |
| WO2006009870A2 (en) | 2006-01-26 |
| WO2006009870A3 (en) | 2006-06-22 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |