ATE148925T1 - Methode zur amplification von ziel-gerichteten segmenten von nukleinsäure durch die ineinandergeschachtelte polymerase-kettenreaktion - Google Patents

Methode zur amplification von ziel-gerichteten segmenten von nukleinsäure durch die ineinandergeschachtelte polymerase-kettenreaktion

Info

Publication number
ATE148925T1
ATE148925T1 AT94903404T AT94903404T ATE148925T1 AT E148925 T1 ATE148925 T1 AT E148925T1 AT 94903404 T AT94903404 T AT 94903404T AT 94903404 T AT94903404 T AT 94903404T AT E148925 T1 ATE148925 T1 AT E148925T1
Authority
AT
Austria
Prior art keywords
amplification
polymerase chain
chain reaction
nucleic acid
nested polymerase
Prior art date
Application number
AT94903404T
Other languages
English (en)
Inventor
Ron Grosz
Mark Anton Jensen
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Application granted granted Critical
Publication of ATE148925T1 publication Critical patent/ATE148925T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Saccharide Compounds (AREA)
AT94903404T 1992-12-09 1993-12-08 Methode zur amplification von ziel-gerichteten segmenten von nukleinsäure durch die ineinandergeschachtelte polymerase-kettenreaktion ATE148925T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/990,297 US5340728A (en) 1992-12-09 1992-12-09 Method for amplification of targeted segments of nucleic acid using nested polymerase chain reaction

Publications (1)

Publication Number Publication Date
ATE148925T1 true ATE148925T1 (de) 1997-02-15

Family

ID=25535998

Family Applications (1)

Application Number Title Priority Date Filing Date
AT94903404T ATE148925T1 (de) 1992-12-09 1993-12-08 Methode zur amplification von ziel-gerichteten segmenten von nukleinsäure durch die ineinandergeschachtelte polymerase-kettenreaktion

Country Status (13)

Country Link
US (1) US5340728A (de)
EP (1) EP0675969B1 (de)
JP (1) JPH08504332A (de)
AT (1) ATE148925T1 (de)
AU (1) AU5736194A (de)
CA (1) CA2151036A1 (de)
DE (1) DE69308162T2 (de)
DK (1) DK0675969T3 (de)
ES (1) ES2097633T3 (de)
GR (1) GR3023325T3 (de)
IL (1) IL107935A0 (de)
MX (1) MX9307788A (de)
WO (1) WO1994013832A1 (de)

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US5705366A (en) * 1994-09-15 1998-01-06 Johnson & Johnson Clinical Diagnostics, Inc. Coamplification of target nucleic acids using volume exclusion agent in reaction composition, test kit and test device useful therefor
CA2204355C (en) 1994-11-02 2001-01-16 Gail Mandel Peripheral nervous system specific sodium channels, dna encoding therefor, crystallization, x-ray diffraction, computer molecular modeling, rational drug design, drug screening, and methods of making and using thereof
US6110665A (en) * 1995-02-14 2000-08-29 University Of Kentucky Research Foundation Sarcocystis neuronadiagnostic primer and its use in methods of equine protozoal myeloencephalitis diagnosis
GB9509112D0 (en) * 1995-05-04 1995-06-28 Scottish Crop Research Inst Polynucleotide
GB9509410D0 (en) * 1995-05-10 1995-07-05 Imperial College Molecular imaging
US5693467A (en) * 1995-05-19 1997-12-02 The American Type Culture Collection Mycoplasma polymerase chain reaction testing system using a set of mixed and single sequence primers
ATE215993T1 (de) * 1995-05-19 2002-04-15 Abbott Lab Nachweis von nukleinsäuren mit breitem dynamikbereich unter verwendung von aggregat- primer-reihen
CN1151269C (zh) * 1995-06-06 2004-05-26 扬·维吉 用于诊断性dna检测的方法
US5882857A (en) * 1995-06-07 1999-03-16 Behringwerke Ag Internal positive controls for nucleic acid amplification
CA2223050A1 (en) * 1995-06-07 1996-12-19 Abbott Laboratories Probe masking method of reducing background in an amplification reaction
AT402203B (de) 1995-06-13 1997-03-25 Himmler Gottfried Dipl Ing Dr Verfahren zur transkriptionsfreien amplifizierung von nucleinsäuren
CZ296930B6 (cs) * 1995-07-28 2006-07-12 Sapporo Breweries Ltd. Zpusob identifikace genetických druhu chmelu
US5747257A (en) * 1996-02-29 1998-05-05 E. I. Du Pont De Nemours And Company Genetic markers and methods for the detection of escherichia coli serotype-0157:H7
US6458530B1 (en) * 1996-04-04 2002-10-01 Affymetrix Inc. Selecting tag nucleic acids
JP4540754B2 (ja) * 1996-06-04 2010-09-08 ユニバーシティ オブ ユタ リサーチ ファウンデーション Ｐｃｒ中のハイブリダイゼーションのモニタリング
US5989873A (en) * 1996-09-24 1999-11-23 Vinayagamoorthy; Thuraiayah Method of detecting gene expression and/or of preventing such expression in cells
US5922538A (en) * 1996-11-08 1999-07-13 E.I. Du Pont De Nemours And Company Genetic markers and methods for the detection of Listeria monocytogenes and Listeria spp
IL120339A0 (en) * 1997-02-27 1997-06-10 Gesher Israel Advanced Biotecs Improved DNA assembly method
US6103468A (en) * 1997-10-07 2000-08-15 Labatt Brewing Company Limited Rapid two-stage polymerase chain reaction method for detection of lactic acid bacteria in beer
US6468743B1 (en) * 1998-05-18 2002-10-22 Conagra Grocery Products Company PCR techniques for detecting microbial contaminants in foodstuffs
US6232104B1 (en) 1999-08-17 2001-05-15 Dade Behring Inc. Detection of differences in nucleic acids by inhibition of spontaneous DNA branch migration
ES2189631B1 (es) * 2001-03-12 2005-01-01 BIOTOOLS BIOTECHNOLOGICAL & MEDICAL LABORATORIES, S.A. Metodo para la amplificacion de adn mediante semi-nested pcr en un solo paso y su empleo en la identificacion de especies de plasmodium.
JP3979024B2 (ja) * 2001-03-29 2007-09-19 日本製紙株式会社 Ｒａｐｄ法による個体識別方法
BR0210610A (pt) * 2001-06-22 2005-04-19 Marshfield Clinic Métodos e oligonucleotìdeos para detecção de salmonella sp., e.coli 0157:h7 e listeria monocitogenes
AU2003240795A1 (en) * 2002-05-24 2003-12-12 Invitrogen Corporation Nested pcr employing degradable primers
EP2031070B1 (de) 2002-12-04 2013-07-17 Life Technologies Corporation Multiplexverstärkung von Polynucleotiden
US20060024690A1 (en) * 2003-09-19 2006-02-02 Kao H P Normalization of data using controls
WO2005029041A2 (en) * 2003-09-19 2005-03-31 Applera Corporation High density sequence detection methods and apparatus
CN1898395B (zh) * 2003-10-13 2014-02-12 戴尔斯瑞克斯实验室 基于引物的核酸的多重扩增的方法和试剂盒
US20050255485A1 (en) * 2004-05-14 2005-11-17 Livak Kenneth J Detection of gene duplications
US20060003337A1 (en) * 2004-06-30 2006-01-05 John Brandis Detection of small RNAS
KR100671501B1 (ko) * 2005-02-28 2007-01-19 삼성에버랜드 주식회사 식중독 검출용 프라이머 및 이를 이용한 세균성 식중독 검출방법
US20060240442A1 (en) * 2005-04-20 2006-10-26 Vevea Dirk N Methods and oligonucleotides for the detection of Salmonella SP., E coli 0157:H7, and Listeria monocytogenes
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FR2926367B1 (fr) * 2008-01-10 2013-01-04 Novaleads Procede fluorimetrique pour evaluer l'influence d'une condition sur un echantillon biologique et ses applications.
EP2438184B1 (de) * 2009-06-02 2014-06-04 Monoquant PTY LTD Verfahren zur amplifikation von nukleinsäuren
CN103917665B (zh) * 2011-11-09 2016-11-16 纳幕尔杜邦公司 用于沙门氏菌检测的序列和它们的用法
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US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
JPH0638759B2 (ja) * 1988-07-29 1994-05-25 株式会社島津製作所 サルモネラ菌検査に用いるｄｎａプローブ及びそれを用いたサルモネラ菌の検出法
DE69031318D1 (de) * 1989-03-17 1997-10-02 Abbott Lab Verfahren und Vorrichtung zur Hybridisierung von Nukleinsäure mit verbesserter Reaktionskinetik
WO1990015881A1 (fr) * 1989-06-12 1990-12-27 Cis Bio International Procede de detection de sequences specifiques d'acides nucleiques et ses applications
EP0409159B1 (de) * 1989-07-18 1998-11-25 Shimadzu Corporation Verfahren zur Untersuchung von durch Mikroorganismen verursachten Lebensmittelvergiftungen und Reagens dafür
US5126239A (en) * 1990-03-14 1992-06-30 E. I. Du Pont De Nemours And Company Process for detecting polymorphisms on the basis of nucleotide differences
JPH0491790A (ja) * 1990-08-02 1992-03-25 Shionogi & Co Ltd ２段階ｐｃｒ法
GB9107124D0 (en) * 1991-04-05 1991-05-22 Dynal As Chemical process

Also Published As

Publication number Publication date
JPH08504332A (ja) 1996-05-14
DE69308162T2 (de) 1997-06-26
CA2151036A1 (en) 1994-06-23
WO1994013832A1 (en) 1994-06-23
ES2097633T3 (es) 1997-04-01
US5340728A (en) 1994-08-23
MX9307788A (es) 1994-07-29
AU5736194A (en) 1994-07-04
EP0675969A1 (de) 1995-10-11
DE69308162D1 (de) 1997-03-27
GR3023325T3 (en) 1997-08-29
IL107935A0 (en) 1994-04-12
EP0675969B1 (de) 1997-02-12
DK0675969T3 (da) 1997-04-28

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