ATE459728T1 - Verfahren zur amplifikation polymerer nukleinsäuren - Google Patents
Verfahren zur amplifikation polymerer nukleinsäurenInfo
- Publication number
- ATE459728T1 ATE459728T1 AT04779936T AT04779936T ATE459728T1 AT E459728 T1 ATE459728 T1 AT E459728T1 AT 04779936 T AT04779936 T AT 04779936T AT 04779936 T AT04779936 T AT 04779936T AT E459728 T1 ATE459728 T1 AT E459728T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acid
- acid polymer
- primer
- primers
- hybridizes
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title abstract 8
- 102000039446 nucleic acids Human genes 0.000 title abstract 8
- 108020004707 nucleic acids Proteins 0.000 title abstract 8
- 238000000034 method Methods 0.000 title abstract 3
- 230000003321 amplification Effects 0.000 title abstract 2
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 2
- 229920000642 polymer Polymers 0.000 abstract 6
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 abstract 3
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 abstract 3
- 239000000203 mixture Substances 0.000 abstract 3
- 230000006820 DNA synthesis Effects 0.000 abstract 2
- 230000001351 cycling effect Effects 0.000 abstract 2
- 239000011541 reaction mixture Substances 0.000 abstract 2
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 238000012986 modification Methods 0.000 abstract 1
- 230000004048 modification Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Macromolecular Compounds Obtained By Forming Nitrogen-Containing Linkages In General (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US49212003P | 2003-08-01 | 2003-08-01 | |
| PCT/US2004/025016 WO2005012499A2 (en) | 2003-08-01 | 2004-08-02 | Methods for amplifying polymeric nucleic acids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE459728T1 true ATE459728T1 (de) | 2010-03-15 |
Family
ID=34115599
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT04779936T ATE459728T1 (de) | 2003-08-01 | 2004-08-02 | Verfahren zur amplifikation polymerer nukleinsäuren |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP1649065B1 (de) |
| JP (1) | JP2007501005A (de) |
| AT (1) | ATE459728T1 (de) |
| AU (1) | AU2004262037A1 (de) |
| CA (1) | CA2533305A1 (de) |
| DE (1) | DE602004025815D1 (de) |
| WO (1) | WO2005012499A2 (de) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8252558B2 (en) | 2006-10-09 | 2012-08-28 | Oxitec Limited | Methods for amplifying and detecting nucleic acid sequences |
| ES2384681T3 (es) | 2008-08-12 | 2012-07-10 | F. Hoffmann-La Roche Ag | Extensión de cebadores correctora |
| WO2011063403A1 (en) | 2009-11-23 | 2011-05-26 | Swift Biosciences, Inc. | Devices to extend single stranded target molecules |
| ES2657988T3 (es) * | 2013-09-13 | 2018-03-07 | F. Hoffmann-La Roche Ag | Aplicación de moléculas de oligo-dT para evitar la generación de productos de PCR de alta masa molecular inducida por transportador poliA |
| GB2527115A (en) * | 2014-06-12 | 2015-12-16 | Vela Operations Pte Ltd | Improved NGS workflow |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6335184B1 (en) | 1993-07-23 | 2002-01-01 | Bio-Rad Laboratories, Inc. | Linked linear amplification of nucleic acids |
| US6093807A (en) * | 1999-03-19 | 2000-07-25 | Isis Pharmaceuticals, Inc. | Sugar-modified 7-deaza-7-substituted oligonucleotides |
| US7205129B1 (en) * | 2000-02-28 | 2007-04-17 | Qiagen Gmbh | Method for reducing artifacts in nucleic acid amplification |
| EP1481088B1 (de) * | 2002-03-01 | 2008-09-17 | Integrated Dna Technologies, Inc. | Polynomiale amplifikation von nukleinsäuren |
| US20030228596A1 (en) * | 2002-03-14 | 2003-12-11 | Qiang Liu | Template-driven nucleic acid amplifications |
-
2004
- 2004-08-02 JP JP2006522135A patent/JP2007501005A/ja active Pending
- 2004-08-02 AT AT04779936T patent/ATE459728T1/de not_active IP Right Cessation
- 2004-08-02 EP EP04779936A patent/EP1649065B1/de not_active Expired - Lifetime
- 2004-08-02 CA CA002533305A patent/CA2533305A1/en not_active Abandoned
- 2004-08-02 AU AU2004262037A patent/AU2004262037A1/en not_active Abandoned
- 2004-08-02 WO PCT/US2004/025016 patent/WO2005012499A2/en not_active Ceased
- 2004-08-02 DE DE602004025815T patent/DE602004025815D1/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| DE602004025815D1 (de) | 2010-04-15 |
| EP1649065B1 (de) | 2010-03-03 |
| EP1649065A4 (de) | 2007-10-10 |
| WO2005012499A2 (en) | 2005-02-10 |
| CA2533305A1 (en) | 2005-02-10 |
| AU2004262037A1 (en) | 2005-02-10 |
| JP2007501005A (ja) | 2007-01-25 |
| WO2005012499A3 (en) | 2005-09-15 |
| EP1649065A2 (de) | 2006-04-26 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |