ATE460472T1 - Verfahren und zusammensetzungen zur synthese von nukleinsäuremolekülen unter verwendung multipler erkennungsstellen - Google Patents
Verfahren und zusammensetzungen zur synthese von nukleinsäuremolekülen unter verwendung multipler erkennungsstellenInfo
- Publication number
- ATE460472T1 ATE460472T1 AT01996076T AT01996076T ATE460472T1 AT E460472 T1 ATE460472 T1 AT E460472T1 AT 01996076 T AT01996076 T AT 01996076T AT 01996076 T AT01996076 T AT 01996076T AT E460472 T1 ATE460472 T1 AT E460472T1
- Authority
- AT
- Austria
- Prior art keywords
- methods
- nucleic acid
- acid molecules
- compositions
- molecules
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 5
- 102000039446 nucleic acids Human genes 0.000 title abstract 5
- 108020004707 nucleic acids Proteins 0.000 title abstract 5
- 150000007523 nucleic acids Chemical class 0.000 title abstract 5
- 239000000203 mixture Substances 0.000 title abstract 4
- 230000015572 biosynthetic process Effects 0.000 title 1
- 238000003786 synthesis reaction Methods 0.000 title 1
- 238000010367 cloning Methods 0.000 abstract 2
- 150000001875 compounds Chemical class 0.000 abstract 2
- 238000005215 recombination Methods 0.000 abstract 2
- 230000006798 recombination Effects 0.000 abstract 2
- 239000013598 vector Substances 0.000 abstract 2
- 101710183280 Topoisomerase Proteins 0.000 abstract 1
- 150000001720 carbohydrates Chemical class 0.000 abstract 1
- 235000014633 carbohydrates Nutrition 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 229940079593 drug Drugs 0.000 abstract 1
- 150000002632 lipids Chemical class 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/90—Isomerases (5.)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
- Hall/Mr Elements (AREA)
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US25451000P | 2000-12-08 | 2000-12-08 | |
| US09/732,914 US7393632B2 (en) | 1999-12-10 | 2000-12-11 | Use of multiple recombination sites with unique specificity in recombinational cloning |
| US31890201P | 2001-09-14 | 2001-09-14 | |
| US32609201P | 2001-09-28 | 2001-09-28 | |
| US33312401P | 2001-11-27 | 2001-11-27 | |
| PCT/US2001/045773 WO2002046372A1 (en) | 2000-12-08 | 2001-12-07 | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE460472T1 true ATE460472T1 (de) | 2010-03-15 |
Family
ID=27540352
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT01996076T ATE460472T1 (de) | 2000-12-08 | 2001-12-07 | Verfahren und zusammensetzungen zur synthese von nukleinsäuremolekülen unter verwendung multipler erkennungsstellen |
Country Status (9)
| Country | Link |
|---|---|
| EP (2) | EP1349917B1 (de) |
| JP (2) | JP4394878B2 (de) |
| CN (1) | CN100357427C (de) |
| AT (1) | ATE460472T1 (de) |
| AU (2) | AU2002227153B2 (de) |
| CA (1) | CA2430947A1 (de) |
| DE (1) | DE60141533D1 (de) |
| NZ (1) | NZ526803A (de) |
| WO (1) | WO2002046372A1 (de) |
Families Citing this family (33)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6720140B1 (en) | 1995-06-07 | 2004-04-13 | Invitrogen Corporation | Recombinational cloning using engineered recombination sites |
| US6143557A (en) | 1995-06-07 | 2000-11-07 | Life Technologies, Inc. | Recombination cloning using engineered recombination sites |
| US7351578B2 (en) | 1999-12-10 | 2008-04-01 | Invitrogen Corp. | Use of multiple recombination sites with unique specificity in recombinational cloning |
| EP2119788A1 (de) | 1999-03-02 | 2009-11-18 | Life Technologies Corporation | Zusammensetzungen und Verfahren zur Verwendung beim Rekombinationsklonen von Nukleinsäuren |
| EP2210948A3 (de) | 1999-12-10 | 2010-10-06 | Life Technologies Corporation | Verwendung von mehrfachen Rekombinationsstellen mit einzigartiger Spezifizität beim Rekombinationsklonen |
| US7198924B2 (en) | 2000-12-11 | 2007-04-03 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
| JP2005532829A (ja) * | 2002-07-18 | 2005-11-04 | インヴィトロジェン コーポレーション | 組換え部位を含むウイルスベクター |
| US7160702B2 (en) | 2002-07-26 | 2007-01-09 | Shuwei Yang | Methods and nucleic acid vectors for rapid expression and screening of CDNA clones |
| US7267950B2 (en) * | 2003-05-01 | 2007-09-11 | Veridex, Lcc | Rapid extraction of RNA from cells and tissues |
| WO2004101800A1 (en) * | 2003-05-15 | 2004-11-25 | Medigene Ag | Dna constructs and vectors for the inducible expression of nucleotide sequences |
| WO2005028615A2 (en) * | 2003-06-26 | 2005-03-31 | Invitrogen Corporation | Methods and compositions for detecting promoter activity and expressing fusion proteins |
| WO2005014796A2 (en) * | 2003-08-08 | 2005-02-17 | Invitrogen Corporation | Methods and compositions for seamless cloning of nucleic acid molecules |
| WO2005054427A2 (en) | 2003-10-22 | 2005-06-16 | Invitrogen Corporation | Target sequences for synthetic molecules |
| US8304189B2 (en) | 2003-12-01 | 2012-11-06 | Life Technologies Corporation | Nucleic acid molecules containing recombination sites and methods of using the same |
| US7622252B2 (en) | 2005-06-10 | 2009-11-24 | Baylor College Of Medicine | Generation of minicircle DNA with physiological supercoiling |
| EP2488641B1 (de) | 2009-10-16 | 2019-01-23 | Baylor College Of Medicine | Supergespulte minicircle-dna für gentherapieanwendungen |
| WO2012024351A2 (en) * | 2010-08-16 | 2012-02-23 | Salk Institute For Biological Studies | Adenoviral assembly method |
| ITMI20110377A1 (it) | 2011-03-10 | 2012-09-11 | Wilic Sarl | Macchina elettrica rotante per aerogeneratore |
| SG11201408736SA (en) * | 2012-06-29 | 2015-03-30 | Massachusetts Inst Technology | Massively parallel combinatorial genetics |
| IN2014DN11250A (de) * | 2012-07-26 | 2015-10-09 | Dow Agrosciences Llc | |
| US9801874B2 (en) * | 2012-11-20 | 2017-10-31 | Spectrum Pharmaceuticals | Method for the preparation of liposome encapsulated vincristine for therapeutic use |
| AU2014236207B2 (en) | 2013-03-14 | 2019-05-23 | Salk Institute For Biological Studies | Oncolytic adenovirus compositions |
| KR20250081944A (ko) | 2014-09-24 | 2025-06-05 | 솔크 인스티튜트 포 바이올로지칼 스터디즈 | 종양 살상 바이러스 및 이의 사용방법 |
| FR3028527A1 (fr) | 2014-11-13 | 2016-05-20 | Pivert | Identification de facteurs de transcription de yarrowia lipolytica |
| US10407676B2 (en) * | 2014-12-09 | 2019-09-10 | Life Technologies Corporation | High efficiency, small volume nucleic acid synthesis |
| CN105039375B (zh) * | 2015-03-11 | 2019-02-05 | 戚智青 | 一种利用蛋白ihf构建克隆载体的方法 |
| TWI678213B (zh) | 2015-07-22 | 2019-12-01 | 美商史倍壯製藥公司 | 用於長春新鹼硫酸鹽脂質體注射之即可使用的調配物 |
| EP4155411A1 (de) | 2016-02-23 | 2023-03-29 | Salk Institute for Biological Studies | Exogene genexpression eines therapeutischen adenovirus mit geringem einfluss auf die virale kinetik |
| WO2017147265A1 (en) | 2016-02-23 | 2017-08-31 | Salk Institute For Biological Studies | High throughput assay for measuring adenovirus replication kinetics |
| EP3532082A4 (de) | 2016-12-12 | 2020-08-26 | Salk Institute for Biological Studies | Auf tumoren gerichtete synthetische adenoviren und verwendungen davon |
| MX2020010499A (es) | 2018-04-09 | 2020-10-28 | Salk Inst For Biological Studi | Composiciones de adenovirus oncoliticas con propiedades aumentadas de replicacion. |
| US20230151391A1 (en) * | 2020-02-14 | 2023-05-18 | Public University Corporation Yokohama City University | Nucleic acid construct that can measure homologous recombination activity, and method for using same |
| CN117092084B (zh) * | 2023-10-20 | 2024-01-12 | 浙江迪福润丝生物科技有限公司 | Wnv蛋白酶抑制剂的筛选方法和抑制效果评价方法 |
Family Cites Families (57)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US5206347A (en) | 1985-08-06 | 1993-04-27 | La Jolla Cancer Research Foundation | Isolation and use of receptors binding to a peptide column |
| US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
| US5374553A (en) | 1986-08-22 | 1994-12-20 | Hoffmann-La Roche Inc. | DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima |
| US4889818A (en) | 1986-08-22 | 1989-12-26 | Cetus Corporation | Purified thermostable enzyme |
| WO1992006200A1 (en) | 1990-09-28 | 1992-04-16 | F. Hoffmann-La-Roche Ag | 5' to 3' exonuclease mutations of thermostable dna polymerases |
| US5079352A (en) | 1986-08-22 | 1992-01-07 | Cetus Corporation | Purified thermostable enzyme |
| US5244797B1 (en) | 1988-01-13 | 1998-08-25 | Life Technologies Inc | Cloned genes encoding reverse transcriptase lacking rnase h activity |
| CA1340807C (en) | 1988-02-24 | 1999-11-02 | Lawrence T. Malek | Nucleic acid amplification process |
| US5223409A (en) | 1988-09-02 | 1993-06-29 | Protein Engineering Corp. | Directed evolution of novel binding proteins |
| US5399346A (en) | 1989-06-14 | 1995-03-21 | The United States Of America As Represented By The Department Of Health And Human Services | Gene therapy |
| US5436146A (en) | 1989-09-07 | 1995-07-25 | The Trustees Of Princeton University | Helper-free stocks of recombinant adeno-associated virus vectors |
| US5225212A (en) | 1989-10-20 | 1993-07-06 | Liposome Technology, Inc. | Microreservoir liposome composition and method |
| US5283173A (en) | 1990-01-24 | 1994-02-01 | The Research Foundation Of State University Of New York | System to detect protein-protein interactions |
| DE69133389T2 (de) | 1990-09-27 | 2005-06-02 | Invitrogen Corp., Carlsbad | Direkte Klonierung von PCR amplifizierten Nukleinsäuren |
| DE553264T1 (de) | 1990-10-05 | 1994-04-28 | Wayne M Barnes | Thermostabile dna polymerase. |
| SE9003978D0 (sv) | 1990-12-13 | 1990-12-13 | Henrik Garoff | Dna expressionssystem baserade paa ett virus replikon |
| US5455166A (en) | 1991-01-31 | 1995-10-03 | Becton, Dickinson And Company | Strand displacement amplification |
| US5871907A (en) | 1991-05-15 | 1999-02-16 | Medical Research Council | Methods for producing members of specific binding pairs |
| US5858657A (en) | 1992-05-15 | 1999-01-12 | Medical Research Council | Methods for producing members of specific binding pairs |
| US6013638A (en) | 1991-10-02 | 2000-01-11 | The United States Of America As Represented By The Department Of Health And Human Services | Adenovirus comprising deletions on the E1A, E1B and E3 regions for transfer of genes to the lung |
| US5733743A (en) | 1992-03-24 | 1998-03-31 | Cambridge Antibody Technology Limited | Methods for producing members of specific binding pairs |
| AU680459B2 (en) | 1992-12-03 | 1997-07-31 | Genzyme Corporation | Gene therapy for cystic fibrosis |
| US5436149A (en) | 1993-02-19 | 1995-07-25 | Barnes; Wayne M. | Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension |
| US5395619A (en) | 1993-03-03 | 1995-03-07 | Liposome Technology, Inc. | Lipid-polymer conjugates and liposomes |
| US5789156A (en) | 1993-06-14 | 1998-08-04 | Basf Ag | Tetracycline-regulated transcriptional inhibitors |
| TW442569B (en) | 1993-10-25 | 2001-06-23 | Canji Inc | Recombinant adenoviral vector |
| US5556772A (en) | 1993-12-08 | 1996-09-17 | Stratagene | Polymerase compositions and uses thereof |
| US5512462A (en) | 1994-02-25 | 1996-04-30 | Hoffmann-La Roche Inc. | Methods and reagents for the polymerase chain reaction amplification of long DNA sequences |
| US5648211A (en) | 1994-04-18 | 1997-07-15 | Becton, Dickinson And Company | Strand displacement amplification using thermophilic enzymes |
| SE9401709D0 (sv) | 1994-05-18 | 1994-05-18 | Mathilda Sjoeberg | Improved alphavirus vectors for expression of heterologous DNA |
| US5912155A (en) | 1994-09-30 | 1999-06-15 | Life Technologies, Inc. | Cloned DNA polymerases from Thermotoga neapolitana |
| US5614365A (en) | 1994-10-17 | 1997-03-25 | President & Fellow Of Harvard College | DNA polymerase having modified nucleotide binding site for DNA sequencing |
| US5998205A (en) | 1994-11-28 | 1999-12-07 | Genetic Therapy, Inc. | Vectors for tissue-specific replication |
| ES2258265T3 (es) | 1994-11-28 | 2006-08-16 | Cell Genesys Inc. | Vectores de replicacion con especificidad tisular. |
| US5766891A (en) | 1994-12-19 | 1998-06-16 | Sloan-Kettering Institute For Cancer Research | Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase |
| US6143557A (en) | 1995-06-07 | 2000-11-07 | Life Technologies, Inc. | Recombination cloning using engineered recombination sites |
| DK0937098T3 (da) | 1995-06-07 | 2002-12-02 | Invitrogen Corp | Rekombinatorisk kloning under anvendelse af gensplejsede rekombinationssites |
| EP0871775A4 (de) | 1995-09-08 | 2002-07-31 | Life Technologies Inc | Klonierte dna-polymerasen von thermotoga und mutanten davon |
| US5622699A (en) | 1995-09-11 | 1997-04-22 | La Jolla Cancer Research Foundation | Method of identifying molecules that home to a selected organ in vivo |
| US5776689A (en) | 1996-07-19 | 1998-07-07 | The Regents Of The University Of California | Protein recruitment system |
| JP2000516468A (ja) | 1996-08-14 | 2000-12-12 | ライフ テクノロジーズ，インコーポレイテッド | 核酸増幅および配列決定のための安定な組成物 |
| US5962303A (en) | 1996-10-15 | 1999-10-05 | Smithkline Beecham Corporation | Topoisomerase III |
| US5827533A (en) | 1997-02-06 | 1998-10-27 | Duke University | Liposomes containing active agents aggregated with lipid surfactants |
| US5851808A (en) | 1997-02-28 | 1998-12-22 | Baylor College Of Medicine | Rapid subcloning using site-specific recombination |
| EP2184289A1 (de) | 1997-04-22 | 2010-05-12 | Life Technologies Corporation | Verfahren zur Herstellung von aus mehreren Untereinheiten zusammengesetzten ASLV-Umkehrtranskriptasen |
| US5885779A (en) | 1997-09-09 | 1999-03-23 | University Of British Columbia | Repressed trans-activator system for characterization of protein-protein interactions |
| EP1025217B1 (de) | 1997-10-24 | 2006-10-04 | Invitrogen Corporation | Rekombinatorisches klonieren unter verwendung von nukleinsaüren mit rekombinationsstellen |
| US6303362B1 (en) | 1998-11-19 | 2001-10-16 | The Board Of Trustees Of The Leland Stanford Junior University | Adenoviral vector and methods for making and using the same |
| NZ518503A (en) * | 1999-10-25 | 2004-02-27 | Invitrogen Corp | Methods of manipulating and sequencing nucleic acid molecules using transposition and recombination |
| WO2001032915A2 (en) | 1999-11-04 | 2001-05-10 | Genentech, Inc. | Reversed-phase hplc assay for plasminogen activators |
| EP2210948A3 (de) * | 1999-12-10 | 2010-10-06 | Life Technologies Corporation | Verwendung von mehrfachen Rekombinationsstellen mit einzigartiger Spezifizität beim Rekombinationsklonen |
| WO2002061034A2 (en) * | 2000-12-08 | 2002-08-08 | Invitrogen Corporation | Compositions and methods for rapidly generating recombinant nucleic acid molecules |
| US9516099B1 (en) | 2014-09-23 | 2016-12-06 | Amazon Technologies, Inc. | Controlling access to digital items based on weather conditions |
| KR102423818B1 (ko) | 2015-12-18 | 2022-07-21 | 삼성전자주식회사 | 정전척 어셈블리 및 그를 포함하는 반도체 제조장치, 그리고 정전척 온도 측정방법 |
-
2001
- 2001-12-07 AT AT01996076T patent/ATE460472T1/de not_active IP Right Cessation
- 2001-12-07 EP EP01996076A patent/EP1349917B1/de not_active Expired - Lifetime
- 2001-12-07 AU AU2002227153A patent/AU2002227153B2/en not_active Ceased
- 2001-12-07 DE DE60141533T patent/DE60141533D1/de not_active Expired - Lifetime
- 2001-12-07 NZ NZ526803A patent/NZ526803A/en unknown
- 2001-12-07 CN CNB01822539XA patent/CN100357427C/zh not_active Expired - Lifetime
- 2001-12-07 CA CA002430947A patent/CA2430947A1/en not_active Abandoned
- 2001-12-07 EP EP09156848A patent/EP2105496B1/de not_active Expired - Lifetime
- 2001-12-07 AU AU2715302A patent/AU2715302A/xx active Pending
- 2001-12-07 WO PCT/US2001/045773 patent/WO2002046372A1/en not_active Ceased
- 2001-12-07 JP JP2002548090A patent/JP4394878B2/ja not_active Expired - Lifetime
-
2009
- 2009-05-26 JP JP2009125918A patent/JP5191953B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| WO2002046372A9 (en) | 2004-04-29 |
| CN1489627A (zh) | 2004-04-14 |
| JP4394878B2 (ja) | 2010-01-06 |
| CN100357427C (zh) | 2007-12-26 |
| JP2009219497A (ja) | 2009-10-01 |
| EP1349917B1 (de) | 2010-03-10 |
| JP5191953B2 (ja) | 2013-05-08 |
| WO2002046372A1 (en) | 2002-06-13 |
| AU2002227153B2 (en) | 2008-01-24 |
| CA2430947A1 (en) | 2002-06-13 |
| DE60141533D1 (de) | 2010-04-22 |
| JP2004522428A (ja) | 2004-07-29 |
| EP1349917A4 (de) | 2004-09-08 |
| EP1349917A1 (de) | 2003-10-08 |
| EP2105496B1 (de) | 2013-02-20 |
| AU2715302A (en) | 2002-06-18 |
| EP2105496A1 (de) | 2009-09-30 |
| NZ526803A (en) | 2005-02-25 |
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