ATE460490T1 - Erhöhte expression von transgenen durch co- exprimierung mit einen suppressor der post- transkriptionelle genabschaltung (ptgs) - Google Patents

Erhöhte expression von transgenen durch co- exprimierung mit einen suppressor der post- transkriptionelle genabschaltung (ptgs)

Info

Publication number
ATE460490T1
ATE460490T1 AT00977696T AT00977696T ATE460490T1 AT E460490 T1 ATE460490 T1 AT E460490T1 AT 00977696 T AT00977696 T AT 00977696T AT 00977696 T AT00977696 T AT 00977696T AT E460490 T1 ATE460490 T1 AT E460490T1
Authority
AT
Austria
Prior art keywords
protein
expression
plant
ptgs
nucleic acid
Prior art date
Application number
AT00977696T
Other languages
English (en)
Inventor
David Baulcombe
Olivier Voinnet
Andrew John Hamilton
Original Assignee
Plant Bioscience Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9927609.9A external-priority patent/GB9927609D0/en
Priority claimed from GB0022960A external-priority patent/GB0022960D0/en
Priority claimed from GB0023668A external-priority patent/GB0023668D0/en
Application filed by Plant Bioscience Ltd filed Critical Plant Bioscience Ltd
Application granted granted Critical
Publication of ATE460490T1 publication Critical patent/ATE460490T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
AT00977696T 1999-11-22 2000-11-22 Erhöhte expression von transgenen durch co- exprimierung mit einen suppressor der post- transkriptionelle genabschaltung (ptgs) ATE460490T1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GBGB9927609.9A GB9927609D0 (en) 1999-11-22 1999-11-22 Enhanced expression
GB0022960A GB0022960D0 (en) 2000-09-19 2000-09-19 Enhanced expression
GB0023668A GB0023668D0 (en) 2000-09-27 2000-09-27 Enhanced expression
PCT/GB2000/004454 WO2001038512A2 (en) 1999-11-22 2000-11-22 Enhanced transgene expression by co-expression with a suppressor of post-transcriptional gene silencing (ptgs)

Publications (1)

Publication Number Publication Date
ATE460490T1 true ATE460490T1 (de) 2010-03-15

Family

ID=27255894

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00977696T ATE460490T1 (de) 1999-11-22 2000-11-22 Erhöhte expression von transgenen durch co- exprimierung mit einen suppressor der post- transkriptionelle genabschaltung (ptgs)

Country Status (12)

Country Link
US (2) US7217854B1 (de)
EP (1) EP1232274B1 (de)
JP (1) JP4884628B2 (de)
KR (1) KR20030004305A (de)
AT (1) ATE460490T1 (de)
AU (1) AU782788B2 (de)
CA (1) CA2390152C (de)
DE (1) DE60043992D1 (de)
IL (1) IL149388A0 (de)
MX (1) MXPA02005089A (de)
NZ (1) NZ518947A (de)
WO (1) WO2001038512A2 (de)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6972349B1 (en) 1999-11-12 2005-12-06 University Of South Carolina Control of post-transcriptional gene silencing in plants
DE60029986T2 (de) * 1999-11-12 2007-09-06 University Of South Carolina Kontrolle der post-transkriptionellen Genabschaltung (gene silencing) in Pflanzen
GB0101513D0 (en) * 2001-01-19 2001-03-07 Scottish Crop Res Inst 'Gene silencing suppressor'
GB0101505D0 (en) * 2001-01-19 2001-03-07 Scottish Crop Res Inst Protection against gene silencing
ATE522130T1 (de) 2002-07-19 2011-09-15 Univ South Carolina Zusammensetzungen und verfahren zur modulation der genexpression in pflanzen
US7683238B2 (en) 2002-11-12 2010-03-23 iBio, Inc. and Fraunhofer USA, Inc. Production of pharmaceutically active proteins in sprouted seedlings
US7692063B2 (en) 2002-11-12 2010-04-06 Ibio, Inc. Production of foreign nucleic acids and polypeptides in sprout systems
ES2531125T3 (es) 2003-02-03 2015-03-10 Ibio Inc Sistema para la expresión de genes en plantas
CA2540668C (en) * 2003-10-01 2010-12-21 Japan Science And Technology Agency Dna fragment, method for producting transformant for producing transformant for protein production and utilization thereof
GB0327919D0 (en) * 2003-12-02 2004-01-07 Plant Bioscience Ltd Enhanced expression
ES2532609T3 (es) * 2004-02-20 2015-03-30 Ibio, Inc. Sistemas y métodos para expresión clonales en plantas
CA3056110C (en) 2004-04-22 2020-07-14 Surinder Pal Singh Synthesis of long-chain polyunsaturated fatty acids by recombinant cells
ES2529572T3 (es) 2004-04-22 2015-02-23 Commonwealth Scientific And Industrial Research Organisation Síntesis de ácidos grasos poliinsaturados de cadena larga por células recombinantes
EP1616959A1 (de) * 2004-07-07 2006-01-18 Icon Genetics AG Biologisch sichere transiente Proteinexpression in Pflanzen
FR2877014B1 (fr) * 2004-10-21 2009-07-10 Inst Rech Pour Le Dev I R D Et Systemes d'expression de proteines recombinantes et leurs applications
FR2880357B1 (fr) 2005-01-05 2010-12-10 Inst Rech Developpement Ird Vecteurs viraux pour la surexpression ou pour l'extinction de genes d'interet chez les plantes et leurs applications
US8222488B2 (en) 2006-02-03 2012-07-17 Texas A&M University System Enhancing expression of value-added genes by transgenic expression of tombusvirus-based P19 gene mutants
RU2009111266A (ru) 2006-08-29 2010-10-10 Коммонвелт Сайентифик энд Индастриал Рисерч Организейшн (AU) Синтез жирных кислот
EP1897953A1 (de) 2006-09-06 2008-03-12 Icon Genetics GmbH Replicon von Potexviren stammend
WO2008036424A2 (en) * 2006-09-22 2008-03-27 Regents Of The University Of California Chemically inducible cucumber mosaic virus protein expression system
EP2118274A4 (de) * 2007-01-29 2010-09-01 Univ Ohio State Res Found System zur genexpression in pflanzen mit einem virenbasierten expressionsvektor
WO2008138896A1 (en) * 2007-05-09 2008-11-20 Devgen N.V. A method for sustainable transgene transcription
WO2010025285A1 (en) * 2008-08-27 2010-03-04 Arizona Board Of Regents For And On Behalf Of Arizona State University A dna replicon system for high-level rapid production of vaccines and monoclonal antibody therapeutics in plants
AU2013204254B8 (en) * 2008-11-18 2015-10-08 Commonwealth Scientific And Industrial Research Organisation Enzymes and methods for producing omega-3 fatty acids
DK2358882T3 (da) 2008-11-18 2017-11-06 Commw Scient Ind Res Org Enzymer og fremgangsmåder til fremstilling af omega-3 fedtsyrer
US8344208B2 (en) * 2009-02-26 2013-01-01 Baylor University Highly efficient suppressor-dependent protein expression in plants with a viral vector
US20110197312A1 (en) * 2010-02-07 2011-08-11 University Of Kuwait Indigenous Viral Satellite RNA Associate with Cucumber Mosaic Virus used as a Biological Control Agent against Tomato Diseases caused by Virus Strains, Viroids, and Fungi
FR2960550A1 (fr) * 2010-05-26 2011-12-02 Inst Rech R Pour Le Dev Ird Moyens pour la production transitoire dans les plantes de proteines recombinantes utilisables notamment en prophylaxie et en therapeutique
FI20100248A0 (fi) 2010-06-11 2010-06-11 Kristiina Maekinen Menetelmä proteiinien tuottamiseksi
CA2824152C (en) 2011-01-17 2022-12-13 Philip Morris Products S.A. Method and system for producing a heterologous polypeptide in a nicotiana tabacum plant
WO2012098111A1 (en) 2011-01-17 2012-07-26 Philip Morris Products S.A. Vectors for nucleic acid expression in plants
KR101334846B1 (ko) * 2011-07-20 2013-11-29 대한민국 벼줄무늬잎마름병 바이러스 유래 ｎｓ3유전자의 신규 용도
EP2798065B1 (de) * 2011-12-27 2019-06-05 Commonwealth Scientific and Industrial Research Organisation Gleichzeitiges gen-silencing sowie unterdrückung von gen-silencing in derselben zelle
US8946460B2 (en) 2012-06-15 2015-02-03 Commonwealth Scientific And Industrial Research Organisation Process for producing polyunsaturated fatty acids in an esterified form
WO2015196250A1 (en) 2014-06-27 2015-12-30 Commonwealth Scientific And Industrial Research Organisation Lipid comprising docosapentaenoic acid
KR102386838B1 (ko) 2013-12-18 2022-04-15 커먼웰쓰 사이언티픽 앤 인더스트리알 리서치 오거니제이션 장쇄 다중불포화 지방산을 포함하는 지질
WO2015168733A1 (en) * 2014-05-05 2015-11-12 Queensland University Of Technology Decoy molecules
WO2019049111A1 (en) 2017-09-11 2019-03-14 R. J. Reynolds Tobacco Company METHODS AND COMPOSITIONS FOR INCREASING THE EXPRESSION OF GENES OF INTEREST IN A PLANT BY CO-EXPRESSION WITH P21
CN113302308A (zh) * 2018-11-13 2021-08-24 马里兰大学派克分院 植物载体、组合物和与其相关的用途
MX2022005644A (es) 2019-11-12 2022-12-13 Univ Maryland Vectores vegetales, composiciones y usos relacionados con los mismos.
CN115851820B (zh) * 2022-12-19 2024-06-18 内蒙古农业大学 一株烟草脉斑驳病毒的应用
CN119410670A (zh) * 2025-01-06 2025-02-11 中国热带农业科学院三亚研究院 斯里兰卡木薯花叶病毒转录激活因子ac2及其应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5939541A (en) * 1997-03-28 1999-08-17 University Of South Carolina Method for enhancing expression of a foreign or endogenous gene product in plants
DE60029986T2 (de) * 1999-11-12 2007-09-06 University Of South Carolina Kontrolle der post-transkriptionellen Genabschaltung (gene silencing) in Pflanzen
US6972349B1 (en) * 1999-11-12 2005-12-06 University Of South Carolina Control of post-transcriptional gene silencing in plants

Also Published As

Publication number Publication date
US7217854B1 (en) 2007-05-15
JP2003514585A (ja) 2003-04-22
KR20030004305A (ko) 2003-01-14
AU782788B2 (en) 2005-08-25
NZ518947A (en) 2004-05-28
IL149388A0 (en) 2002-11-10
US8871997B2 (en) 2014-10-28
CA2390152C (en) 2012-08-28
MXPA02005089A (es) 2003-01-28
WO2001038512A3 (en) 2002-03-28
EP1232274A2 (de) 2002-08-21
DE60043992D1 (de) 2010-04-22
US20070292862A1 (en) 2007-12-20
AU1533401A (en) 2001-06-04
CA2390152A1 (en) 2001-05-31
WO2001038512A2 (en) 2001-05-31
EP1232274B1 (de) 2010-03-10
JP4884628B2 (ja) 2012-02-29

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