ATE462011T1 - ENZYMATIC REMOVAL OF DIGLYCERIDES FROM COOKING OILS - Google Patents

ENZYMATIC REMOVAL OF DIGLYCERIDES FROM COOKING OILS

Info

Publication number
ATE462011T1
ATE462011T1 AT07017066T AT07017066T ATE462011T1 AT E462011 T1 ATE462011 T1 AT E462011T1 AT 07017066 T AT07017066 T AT 07017066T AT 07017066 T AT07017066 T AT 07017066T AT E462011 T1 ATE462011 T1 AT E462011T1
Authority
AT
Austria
Prior art keywords
enzyme
variant
amino acid
optionally
acyltransferase
Prior art date
Application number
AT07017066T
Other languages
German (de)
Inventor
Anna Cecilie Kristensen
Paul Wassell
Joern Dalgaard Mikkelsen
Joern Borch Soee
Original Assignee
Danisco
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/IB2004/000655 external-priority patent/WO2004064537A2/en
Priority claimed from US10/898,775 external-priority patent/US7718408B2/en
Application filed by Danisco filed Critical Danisco
Application granted granted Critical
Publication of ATE462011T1 publication Critical patent/ATE462011T1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6418—Fatty acids by hydrolysis of fatty acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6458—Glycerides by transesterification, e.g. interesterification, ester interchange, alcoholysis or acidolysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01073—Diacylglycerol-sterol O-acyltransferase (2.3.1.73)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01003—Triacylglycerol lipase (3.1.1.3)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Fats And Perfumes (AREA)

Abstract

The present invention relates to a method of producing a variant glycolipid acyltransferase enzyme comprising: (a) selecting a parent enzyme which is a glycolipid acyltransferase enzyme characterised in that the enzyme comprises the amino acid sequence motif GDSX, wherein X is one or more of the following amino acid residues L, A, V, I, F, Y, H, Q, T, N, M or S; (b) modifying one or more amino acids to produce a variant glycolipid acyltransferase; (c) testing the variant glycolipid acyltransferase for transferase activity, an optionally hydrolytic activity, on a galactolipid substrate, and optionally a phospholipid substrate and/or optionally a triglyceride substrate; (d) selecting a variant enzyme with an enhanced activity towards galactolipids compared with the parent enzyme; and optionally (e) preparing a quantity of the variant enzyme. The present invention further relates to variant lipid acyltransferase enzyme characterised in that the enzyme comprises the amino acid sequence motif GDSX, wherein X is one or more of the following amino acid residues L, A, V, I, F, Y, H, Q, T, N, M or S, and wherein the variant enzyme comprises one or more amino acid modifications compared with a parent sequence at any one or more of the amino acid residues defined in set 2, set 4, set 6 or set 7.
AT07017066T 2003-12-24 2004-12-23 ENZYMATIC REMOVAL OF DIGLYCERIDES FROM COOKING OILS ATE462011T1 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GBGB0330016.7A GB0330016D0 (en) 2003-12-24 2003-12-24 Method
PCT/IB2004/000655 WO2004064537A2 (en) 2003-01-17 2004-01-15 Method for the in situ production of an emulsifier in foodstuff
GBGB0416023.0A GB0416023D0 (en) 2003-12-24 2004-07-16 Method
US10/898,775 US7718408B2 (en) 2003-12-24 2004-07-26 Method

Publications (1)

Publication Number Publication Date
ATE462011T1 true ATE462011T1 (en) 2010-04-15

Family

ID=30776521

Family Applications (2)

Application Number Title Priority Date Filing Date
AT07017066T ATE462011T1 (en) 2003-12-24 2004-12-23 ENZYMATIC REMOVAL OF DIGLYCERIDES FROM COOKING OILS
AT04806534T ATE376593T1 (en) 2003-12-24 2004-12-23 ENZYMATIC TREATMENT OF OILS

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT04806534T ATE376593T1 (en) 2003-12-24 2004-12-23 ENZYMATIC TREATMENT OF OILS

Country Status (9)

Country Link
JP (1) JP2010142243A (en)
CN (2) CN103275944B (en)
AT (2) ATE462011T1 (en)
DE (1) DE602004026229D1 (en)
DK (1) DK1704236T3 (en)
GB (3) GB0330016D0 (en)
NZ (1) NZ547085A (en)
RU (2) RU2377307C2 (en)
ZA (1) ZA200605158B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114958800B (en) * 2022-06-24 2023-08-25 北京脉道生物药品制造有限公司 Taq DNA polymerase mutant capable of tolerating blood or blood product inhibition and application thereof

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6406723B1 (en) * 1997-04-09 2002-06-18 Danisco A/S Method for preparing flour doughs and products made from such doughs using glycerol oxidase and lipase
US5756328A (en) * 1995-12-20 1998-05-26 Cornell Research Foundation, Inc. Acyltransferase and cDNA encoding acyltransferase
ATE231186T1 (en) * 1998-07-21 2003-02-15 Danisco GROCERIES
RU2235775C2 (en) * 1998-11-27 2004-09-10 Новозимс А/С Method for preparing lipolytic enzyme variant and lipolytic enzyme (variants)
DE50115482D1 (en) * 2001-07-11 2010-06-24 Cognis Ip Man Gmbh Lipase / acyltransferase from Candida parapsilosis
MXPA06001719A (en) * 2003-08-11 2006-05-19 Codexis Inc Improved glucose dehydrogenase polypeptides and related polynucleotides.

Also Published As

Publication number Publication date
CN103275944A (en) 2013-09-04
DE602004026229D1 (en) 2010-05-06
CN103275944B (en) 2016-12-07
ATE376593T1 (en) 2007-11-15
RU2377307C2 (en) 2009-12-27
JP2010142243A (en) 2010-07-01
RU2006126654A (en) 2008-01-27
RU2377300C2 (en) 2009-12-27
HK1106953A1 (en) 2008-03-20
GB0415999D0 (en) 2004-08-18
DK1704236T3 (en) 2018-01-08
CN101676390A (en) 2010-03-24
NZ547085A (en) 2009-11-27
RU2006126715A (en) 2008-01-27
GB0330016D0 (en) 2004-01-28
ZA200605158B (en) 2008-04-30
GB0416023D0 (en) 2004-08-18

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