ATE462785T1 - Methode zur trennung und aufreinigung von nukleinsäuren - Google Patents
Methode zur trennung und aufreinigung von nukleinsäurenInfo
- Publication number
- ATE462785T1 ATE462785T1 AT03008719T AT03008719T ATE462785T1 AT E462785 T1 ATE462785 T1 AT E462785T1 AT 03008719 T AT03008719 T AT 03008719T AT 03008719 T AT03008719 T AT 03008719T AT E462785 T1 ATE462785 T1 AT E462785T1
- Authority
- AT
- Austria
- Prior art keywords
- solid phase
- nucleic acids
- nucleic acid
- isolating
- purification
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title abstract 10
- 102000039446 nucleic acids Human genes 0.000 title abstract 10
- 108020004707 nucleic acids Proteins 0.000 title abstract 10
- 238000000034 method Methods 0.000 title abstract 5
- 238000000746 purification Methods 0.000 title abstract 3
- 238000000926 separation method Methods 0.000 title 1
- 239000007790 solid phase Substances 0.000 abstract 7
- 238000002955 isolation Methods 0.000 abstract 2
- 229920000642 polymer Polymers 0.000 abstract 2
- 238000005406 washing Methods 0.000 abstract 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical group O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6846—Common amplification features
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Saccharide Compounds (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2002201106A JP3580801B2 (ja) | 2001-08-01 | 2002-07-10 | 核酸の分離精製方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE462785T1 true ATE462785T1 (de) | 2010-04-15 |
Family
ID=29728454
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT03008719T ATE462785T1 (de) | 2002-07-10 | 2003-04-16 | Methode zur trennung und aufreinigung von nukleinsäuren |
Country Status (3)
| Country | Link |
|---|---|
| EP (1) | EP1380642B1 (de) |
| AT (1) | ATE462785T1 (de) |
| DE (1) | DE60331879D1 (de) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1727900A4 (de) * | 2004-03-26 | 2008-05-28 | Fujifilm Corp | Verfahren zur selektiven trennung und reinigungvon rna sowie verfahren zur trennung und reinigung von nukleinsäure |
| JP2006238816A (ja) * | 2005-03-04 | 2006-09-14 | Fuji Photo Film Co Ltd | 核酸の分離精製方法 |
| JP2006238822A (ja) * | 2005-03-04 | 2006-09-14 | Fuji Photo Film Co Ltd | 核酸の分離精製方法 |
| JP4956727B2 (ja) | 2005-08-30 | 2012-06-20 | 倉敷紡績株式会社 | Ｒｎａ分離精製方法 |
| JP5766178B2 (ja) | 2009-03-24 | 2015-08-19 | ザ・ユニバーシティ・オブ・シカゴＴｈｅ Ｕｎｉｖｅｒｓｉｔｙ Ｏｆ Ｃｈｉｃａｇｏ | ＳｌｉｐＣｈｉｐ装置および方法 |
| US10196700B2 (en) | 2009-03-24 | 2019-02-05 | University Of Chicago | Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes |
| US9464319B2 (en) | 2009-03-24 | 2016-10-11 | California Institute Of Technology | Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes |
| US9447461B2 (en) | 2009-03-24 | 2016-09-20 | California Institute Of Technology | Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes |
| JP6183471B2 (ja) * | 2014-01-31 | 2017-08-23 | 凸版印刷株式会社 | 生体分子解析キット及び生体分子解析方法 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS52117969A (en) * | 1976-03-31 | 1977-10-03 | Nippon Sheet Glass Co Ltd | Method of coating of plastic article belonging to cellulose series |
| CH653043A5 (en) * | 1982-10-05 | 1985-12-13 | Gurit Worbla Ag | Process for the finishing of the surfaces of mouldings made from cellulose esters |
| JPS614959A (ja) * | 1984-06-19 | 1986-01-10 | Fuji Photo Film Co Ltd | 一体型多層分析要素 |
| EP0630974A3 (de) * | 1993-06-25 | 1995-11-15 | Clinical Diagnostic Syst | Verfahren und Testkit zum Nachweis von anorganischen Orthophosphat bei der Amplifizierung von Zielnukleinsäuren. |
| US5804684A (en) * | 1995-08-24 | 1998-09-08 | The Theobald Smith Research Institute, Inc. | Method for isolating nucleic acids |
| JP3890556B2 (ja) * | 2001-11-28 | 2007-03-07 | 富士フイルム株式会社 | 核酸の分離精製方法及び核酸分離精製ユニット |
-
2003
- 2003-04-16 DE DE60331879T patent/DE60331879D1/de not_active Expired - Fee Related
- 2003-04-16 EP EP03008719A patent/EP1380642B1/de not_active Expired - Lifetime
- 2003-04-16 AT AT03008719T patent/ATE462785T1/de not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| EP1380642A1 (de) | 2004-01-14 |
| DE60331879D1 (de) | 2010-05-12 |
| EP1380642B1 (de) | 2010-03-31 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |