ATE464560T1 - Atp-basierter assay zur bestimmung der proliferation hämatopoetischer stamm- und vorläuferzellen - Google Patents

Atp-basierter assay zur bestimmung der proliferation hämatopoetischer stamm- und vorläuferzellen

Info

Publication number
ATE464560T1
ATE464560T1 AT02770372T AT02770372T ATE464560T1 AT E464560 T1 ATE464560 T1 AT E464560T1 AT 02770372 T AT02770372 T AT 02770372T AT 02770372 T AT02770372 T AT 02770372T AT E464560 T1 ATE464560 T1 AT E464560T1
Authority
AT
Austria
Prior art keywords
cells
present
progressor
atp
based assay
Prior art date
Application number
AT02770372T
Other languages
English (en)
Inventor
Ivan N Rich
Original Assignee
Ivan N Rich
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ivan N Rich filed Critical Ivan N Rich
Application granted granted Critical
Publication of ATE464560T1 publication Critical patent/ATE464560T1/de

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5073—Stem cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/20—Screening for compounds of potential therapeutic value cell-free systems
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
    • Y10T436/108331—Preservative, buffer, anticoagulant or diluent

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Organic Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT02770372T 2001-01-29 2002-01-29 Atp-basierter assay zur bestimmung der proliferation hämatopoetischer stamm- und vorläuferzellen ATE464560T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US26479601P 2001-01-29 2001-01-29
PCT/US2002/002458 WO2003004995A2 (en) 2001-01-29 2002-01-29 High-throughput stem cell assay of hematopoietic stem and progenitor cell proliferation

Publications (1)

Publication Number Publication Date
ATE464560T1 true ATE464560T1 (de) 2010-04-15

Family

ID=23007644

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02770372T ATE464560T1 (de) 2001-01-29 2002-01-29 Atp-basierter assay zur bestimmung der proliferation hämatopoetischer stamm- und vorläuferzellen

Country Status (7)

Country Link
US (3) US7354729B2 (de)
EP (1) EP1364197B1 (de)
AT (1) ATE464560T1 (de)
AU (1) AU2002335610C1 (de)
CA (1) CA2437084C (de)
DE (1) DE60235967D1 (de)
WO (1) WO2003004995A2 (de)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1364197B1 (de) * 2001-01-29 2010-04-14 Ivan N. Rich ATP-basierter Assay zur Bestimmung der Proliferation hämatopoetischer Stamm- und Vorläuferzellen
US7989178B2 (en) * 2001-01-29 2011-08-02 Hemogenix, Inc. Colony assay miniaturization with enumeration output
US7354730B2 (en) * 2002-01-29 2008-04-08 Hemogenix, Inc. High-throughput assay of hematopoietic stem and progenitor cell proliferation
US7666615B2 (en) * 2001-01-29 2010-02-23 Hemogenix, Inc. High-throughput assay of hematopoietic stem and progenitor cell proliferation
AU2003258319B2 (en) * 2002-08-21 2009-09-17 Hemogenix, Inc. High-throughput assay of hematopoietic stem and progenitor cell proliferation
CA2548580A1 (en) * 2003-04-03 2004-10-21 Neostem, Inc. Elective collection and banking of autologous peripheral blood stem cells
JP5191731B2 (ja) 2004-08-12 2013-05-08 ニューロ プローブ インコーポレイテッド 点源拡散細胞活性分析装置および方法
WO2006031524A2 (en) * 2004-09-10 2006-03-23 The Regents Of The Unversity Of Colorado Early detection of hemangiosarcoma and angiosarcoma
WO2006052852A2 (en) * 2004-11-04 2006-05-18 Hemogenix, Inc. Kit for in process quality control to predict and determine engraftment and multilineage reconstitution potential of hematopoietic cells and cord blood storage
CN106434527A (zh) 2006-04-14 2017-02-22 安斯泰来再生医药协会 血管瘤集落形成细胞
US20080038231A1 (en) 2006-06-15 2008-02-14 Rodgerson Denis O Processing procedure for peripheral blood stem cells
US20090028834A1 (en) * 2007-07-27 2009-01-29 Hal Siegel Methods and compositions for stimulating the proliferation or differentiation of stem cells with substance P or an analog thereof
CA2743697A1 (en) * 2007-11-30 2009-06-11 New York Medical College Methods of isolating non-senescent cardiac stem cells and uses thereof
US8512696B2 (en) 2007-11-30 2013-08-20 Autologous, Llc Methods of isolating non-senescent cardiac stem cells and uses thereof
WO2009137836A2 (en) * 2008-05-09 2009-11-12 Hemogenix, Inc. Cell potency assay
US20100003674A1 (en) * 2008-07-03 2010-01-07 Cope Frederick O Adult stem cells, molecular signatures, and applications in the evaluation, diagnosis, and therapy of mammalian conditions
US9273285B1 (en) * 2008-08-25 2016-03-01 Stemcell Technologies Inc. Shorter colony-forming cell assay
KR20180086301A (ko) 2009-12-04 2018-07-30 스템 셀 앤드 리제너러티브 메디신 인터내셔날, 인크. 기질 없는 조건 하에서 인간 배아줄기세포로부터 기능적 거핵구 및 혈소판의 대규모 생성
US8961956B2 (en) 2011-11-30 2015-02-24 Ocata Therapeutics, Inc. Mesenchymal stromal cells and uses related thereto
SI2785359T1 (sl) 2011-11-30 2018-11-30 Astellas Institute For Regenerative Medicine Mezenhimske stromalne celice in uporabe povezane z njimi
JP6057574B2 (ja) * 2012-07-11 2017-01-11 キヤノン株式会社 造血幹細胞識別用プローブ
HK1208054A1 (en) 2012-07-12 2016-02-19 爱姆斯坦生物技术公司 Mesenchymal-like stem cells derived from human embryonic stem cells, methods and uses thereof
CA2896053A1 (en) 2012-12-21 2014-06-26 Ocata Therapeutics, Inc. Methods for production of platelets from pluripotent stem cells and compositions thereof
US10384207B2 (en) 2015-07-21 2019-08-20 Neuro Probe Incorporated Assay apparatus and methods
US11628190B2 (en) * 2015-08-28 2023-04-18 University Of South Florida Plasma derived from human umbilical cord blood for the treatment of neurodegenerative disorders
CN105670992A (zh) * 2016-04-20 2016-06-15 广东常青生命科学研究有限公司 一种体外人工分离淋巴细胞及冷冻保存的方法
CN109948553B (zh) * 2019-03-20 2020-07-24 北京航空航天大学 一种多尺度密集人群计数方法
CN111548993A (zh) * 2020-05-29 2020-08-18 广州市天河诺亚生物工程有限公司 一种脐血单个核细胞的分离方法及其应用
CO2020015994A1 (es) 2020-12-18 2022-06-21 Inst Distrital De Ciencia Biotecnologia E Innovacion En Salud Idcbis Método in vitro para evaluación funcional de progenitores hematopoyéticos

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5328844A (en) * 1990-05-09 1994-07-12 University Of Colorado Foundation, Inc. Culture media for mammalian cells
US5283179A (en) 1990-09-10 1994-02-01 Promega Corporation Luciferase assay method
AU1469292A (en) 1991-01-18 1992-08-27 Oncogene Science, Inc. Methods of transcriptionally modulating expression of growth factor genes and growth factor receptor genes
EP1361273A3 (de) * 1992-11-24 2004-02-11 G.D. Searle & Co. Interleukin-3 (iL-3) mit mehrfachen mutationen Polypeptide
AU6128794A (en) 1993-01-27 1994-08-15 Batle Luminetrics Enterprises, Inc. Methods and kits for determining effects of anti-cancer agents on cancer cells
US5580724A (en) * 1994-03-25 1996-12-03 Board Of Regents, The University Of Texas System Differential expansion of fetal stem cells in maternal circulation for use in prenatal genetic analysis
US5733541A (en) * 1995-04-21 1998-03-31 The Regent Of The University Of Michigan Hematopoietic cells: compositions and methods
AU733714B2 (en) * 1996-08-27 2001-05-24 Hemosol Inc. Enhanced stimulation of erythropoiesis
JPH10136978A (ja) 1996-11-08 1998-05-26 Otsuka Pharmaceut Co Ltd 造血幹細胞の培養方法
GB9626932D0 (en) * 1996-12-24 1997-02-12 Lumitech Limited Assay methods and kits therefor
US5854010A (en) * 1997-03-10 1998-12-29 Denison; Michael S. Bioassay for detecting 2,3,7,8-tetrachlorodibenzo-para-dioxin and TCDD-like compounds and novel recombinant cell line useful therefor
US6824973B2 (en) 2000-02-03 2004-11-30 Kirin Beer Kabushiki Kaisha Method of promoting stem cell proliferation or survival by contacting a cell with a stem cell factor-like polypeptide
US7354730B2 (en) 2002-01-29 2008-04-08 Hemogenix, Inc. High-throughput assay of hematopoietic stem and progenitor cell proliferation
EP1364197B1 (de) * 2001-01-29 2010-04-14 Ivan N. Rich ATP-basierter Assay zur Bestimmung der Proliferation hämatopoetischer Stamm- und Vorläuferzellen
AU2003258319B2 (en) 2002-08-21 2009-09-17 Hemogenix, Inc. High-throughput assay of hematopoietic stem and progenitor cell proliferation

Also Published As

Publication number Publication date
US7700354B2 (en) 2010-04-20
US7709258B2 (en) 2010-05-04
WO2003004995A3 (en) 2003-05-22
US20020146680A1 (en) 2002-10-10
DE60235967D1 (de) 2010-05-27
CA2437084C (en) 2010-12-21
AU2002335610C1 (en) 2008-09-25
US20080160563A1 (en) 2008-07-03
US20080160564A1 (en) 2008-07-03
AU2002335610B2 (en) 2008-04-17
EP1364197B1 (de) 2010-04-14
EP1364197A2 (de) 2003-11-26
EP1364197A4 (de) 2005-08-17
WO2003004995A2 (en) 2003-01-16
CA2437084A1 (en) 2003-01-16
US7354729B2 (en) 2008-04-08

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